Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,299 A→G 7.3% intergenic (‑34/+17) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,2990AG 7.3% 348.3 / 13.0 122intergenic (‑34/+17)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (60/53);  new base G (3/6);  total (63/59)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 123 total aligned reads displayed.

TGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGA  >  NC_000913/3918151‑3918448
                                                                                                                                                    |                                                                                                                                                     
tGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAg                                                                                                                                                      >  1:1589992/1‑150 (MQ=255)
  cGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                                                                    >  1:209685/1‑150 (MQ=255)
  cGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                                                                    <  2:1394324/150‑1 (MQ=255)
  cGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                                                                    <  2:223568/150‑1 (MQ=255)
    aTCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTAAGTGATCTTTTGCGGGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTAGGGCAGGCCGCAAGGCat                                                                                                                                                  <  1:1259258/150‑2 (MQ=255)
        tGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccg                                                                                                                                              <  2:928/150‑1 (MQ=255)
         gcgcTTTACGCATTTTGGAAGCGGCGACCTTCTCCCTCGCTGTACTGAGCTATTGCGTGTTCTTGACGCTTGCGATCTTACTACGTATCGCTTCTGCGCCGGCCATGAGCTGCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgc                                                                                                                                             <  1:313936/150‑1 (MQ=255)
         gCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCACTGCCTTGCGGCTTGCCTTAGGGCAGgccgc                                                                                                                                             <  1:995960/150‑1 (MQ=255)
            aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                                                                          <  1:1236971/150‑1 (MQ=255)
            aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTGCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAg                                                                                                                                          <  1:1336138/150‑1 (MQ=255)
             tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                                                                         <  2:503443/150‑2 (MQ=255)
             tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                                                                         <  1:1072653/150‑2 (MQ=255)
                    aTTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                                                                  >  2:285540/1‑150 (MQ=255)
                     ttttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCa                                                                                                                                 >  2:789098/1‑150 (MQ=255)
                        tGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGa                                                                                                                              >  2:1462423/1‑150 (MQ=255)
                          gAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAtt                                                                                                                            >  2:1566653/1‑150 (MQ=255)
                              cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                                                                        <  2:877859/150‑1 (MQ=255)
                               ggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                       >  2:1686791/1‑150 (MQ=255)
                                      aTCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                                                                                <  1:1613392/150‑1 (MQ=255)
                                        ctcCATCGCTTTAGTGATCTTTTGCGGGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                              <  2:782703/150‑1 (MQ=255)
                                         tcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                                                                             <  1:94629/150‑1 (MQ=255)
                                         tcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                                                                             >  1:1534166/1‑150 (MQ=255)
                                          ccATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                                                                            >  2:1104933/1‑150 (MQ=255)
                                            aTCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAt                                                                                                          >  1:773596/1‑150 (MQ=255)
                                             tCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATc                                                                                                         <  1:678693/150‑1 (MQ=255)
                                             tCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATc                                                                                                         <  1:672534/150‑1 (MQ=255)
                                               gCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGa                                                                                                       >  2:589860/1‑150 (MQ=255)
                                                cTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAg                                                                                                      >  2:1546939/1‑150 (MQ=255)
                                                   tAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGAt                                                                                                   >  1:799122/1‑150 (MQ=255)
                                                    aGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGACAGACGTTACCAGGAATGTGTTGCTTTTAATGAATCGatgatg                                                                                                  >  1:1299291/1‑150 (MQ=255)
                                                      tgctCTTTTGCGTGTTCTGGACGCTTGCGATATTACTACGTATCTATATTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGTAATCGAGGATGcc                                                                                                <  2:1609204/147‑1 (MQ=255)
                                                          cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTc                                                                                            <  2:1589992/150‑1 (MQ=255)
                                                          cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAACCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAACGAATCGAGCAAGCCttta                                                                                            >  1:1426857/1‑149 (MQ=255)
                                                             ttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGCGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                                                                         >  1:999616/1‑150 (MQ=255)
                                                             ttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                                                                         <  1:758442/150‑1 (MQ=255)
                                                              tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCt                                                                                        >  2:1034364/1‑150 (MQ=255)
                                                                   gtTCTGGACGCTTGCGATCTTACTTCGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCt                                                                                   <  2:302035/150‑1 (MQ=255)
                                                                   gtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAGCGAGGATGCCTTTCAGCTTGCCt                                                                                   >  2:679903/1‑150 (MQ=255)
                                                                    ttCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCtt                                                                                  <  1:1177773/150‑1 (MQ=255)
                                                                     gcTGGACGCTTGCGATCTTACTAAGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTc                                                                                 <  1:251879/149‑1 (MQ=255)
                                                                      cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCg                                                                                <  2:1389237/150‑1 (MQ=255)
                                                                      cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCg                                                                                <  2:1357052/150‑1 (MQ=255)
                                                                        ggACGCTTGCGATCTTACTACGTATCTCTTATGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCAGCCCAAAGGCACGCCCCCACACGTAACCAGGAATGGGTTGCTTTTAACGAATCGACGATGCCTTTCATCTAGCCTTCGAt                                                                              >  1:1492365/1‑150 (MQ=255)
                                                                             cTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgt                                                                         >  1:398431/1‑150 (MQ=255)
                                                                               tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                       <  2:718181/150‑1 (MQ=255)
                                                                                gCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTcgtcgt                                                                      >  1:1234649/1‑150 (MQ=255)
                                                                                  gATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTg                                                                    <  2:1534166/150‑1 (MQ=255)
                                                                                     cTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCTGGATTGGGTTGCTTTGAAGGAATCGAGGATGTCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg                                                                 <  1:225933/150‑1 (MQ=255)
                                                                                       tactacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGcc                                                               >  2:762154/1‑150 (MQ=255)
                                                                                          tacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAcc                                                            <  2:519024/150‑1 (MQ=255)
                                                                                              tATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                                                                              <  2:1220849/128‑1 (MQ=255)
                                                                                                 ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGTTCTgg                                                     >  2:220866/1‑150 (MQ=255)
                                                                                                 ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                     >  2:1612533/1‑150 (MQ=255)
                                                                                                  tctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                    <  1:1082210/150‑1 (MQ=255)
                                                                                                  tctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCCGCATGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCGTTAAGCGTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCGGGt                                                    >  2:1086232/1‑150 (MQ=255)
                                                                                                    ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAcc                                                            >  1:1483434/1‑140 (MQ=255)
                                                                                                    ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAcc                                                            <  2:1483434/140‑1 (MQ=255)
                                                                                                       tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAtc                                               <  2:430251/150‑1 (MQ=255)
                                                                                                           ccGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCtt                                           <  2:1087048/150‑1 (MQ=255)
                                                                                                            cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTg                                          <  2:1193838/150‑1 (MQ=255)
                                                                                                            cGGCCATGAGCATCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTGGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTg                                          <  1:1055464/150‑1 (MQ=255)
                                                                                                             ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                    <  1:512679/139‑1 (MQ=255)
                                                                                                             ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                    >  2:512679/1‑139 (MQ=255)
                                                                                                             ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGc                                         >  2:630884/1‑150 (MQ=255)
                                                                                                             ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCAAAGGCAAGCCGCCAGACGTTCCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTGATCTCTTGc                                         <  2:903346/150‑1 (MQ=255)
                                                                                                              gCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCa                                        <  1:195555/150‑1 (MQ=255)
                                                                                                                cATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATc                                      >  2:236489/1‑150 (MQ=255)
                                                                                                                  tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCaa                                    >  1:789045/1‑150 (MQ=255)
                                                                                                                  tGAGCTTCTCCTCAATGCCTGGCGGCCGGCCCTAAGGCAAGCCGCCCGACGGTACCAGGATTGGGTTGCTTTGAAGGATTCGAGGATGCCTTTCATCTTGCCTTCGATGTCGTCGTTGTAGCCCCCGGTAGTGTTGATCTCTTgaagcca                                    >  2:1311841/1‑144 (MQ=255)
                                                                                                                    aGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACg                                  >  2:355815/1‑150 (MQ=255)
                                                                                                                     gCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTGGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACgg                                 >  2:1632169/1‑150 (MQ=255)
                                                                                                                       ttCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAg                               >  2:509185/1‑150 (MQ=255)
                                                                                                                       ttCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAg                               >  2:1192344/1‑150 (MQ=255)
                                                                                                                          tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGAGGTTACCAGGATTGGGTTGCTTTGAAGGAAGCGAGGATGCCTTTCCGCTTGCCTTCGATTTCGTCGTTGTCGCCTCCGGTCTGGTTGATCTCTTGCATCAACGGAGCGt                            >  1:251953/1‑150 (MQ=255)
                                                                                                                           cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCAAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGAAGCCTTTAAGCTTCCCTTCGATTTAGTCGGTGTAGCCCCCCGTCTGGTTGATCTCTTGCATGTACGGAGCGTg                           >  2:820303/1‑150 (MQ=255)
                                                                                                                            ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                          <  1:1034364/150‑1 (MQ=255)
                                                                                                                            ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                          >  2:156395/1‑150 (MQ=255)
                                                                                                                            ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCGTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                          >  1:702941/1‑150 (MQ=255)
                                                                                                                             tcAATGCGTTGCGGCCTGCGCTAAGGCAAGCCGCCAGACGTTACGTGGTATGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAGCGGGCTGGTTGATCTCTTGCATCAACGGAGCGTGAt                         <  2:465507/150‑1 (MQ=255)
                                                                                                                              cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                        >  2:650215/1‑150 (MQ=255)
                                                                                                                              cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                        <  1:1462423/150‑1 (MQ=255)
                                                                                                                              cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                        <  2:1018081/150‑1 (MQ=255)
                                                                                                                               aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTTGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCa                       >  2:143279/1‑150 (MQ=255)
                                                                                                                               aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCa                       <  1:1248537/150‑1 (MQ=255)
                                                                                                                               aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCa                       <  2:1275886/150‑1 (MQ=255)
                                                                                                                                aTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCAc                      <  1:200560/150‑1 (MQ=255)
                                                                                                                                   ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAGCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                   <  1:1432671/150‑1 (MQ=255)
                                                                                                                                    ctggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTc                  >  1:556596/4‑150 (MQ=255)
                                                                                                                                    cTTGGGGCCTGACCGAAGGAAATACGCCAGAAGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGACTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACAGGTCTGGTTGATCTCTTGCATAAACGGAGCGTGATCACGGTc                  <  2:1492365/150‑1 (MQ=255)
                                                                                                                                    cTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTc                  <  2:773596/150‑1 (MQ=255)
                                                                                                                                      ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTCTAGCCACCGGTCTGGTTGCTCTCTTCCATCAACGGAGCGTGATCAGGGTCGa                >  2:1077038/2‑150 (MQ=255)
                                                                                                                                       gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTATGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGAc               >  1:476164/1‑150 (MQ=255)
                                                                                                                                        cGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg              >  1:1218476/1‑150 (MQ=255)
                                                                                                                                          gCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTa            >  1:1199610/1‑150 (MQ=255)
                                                                                                                                          gCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTa            >  1:1059959/1‑150 (MQ=255)
                                                                                                                                               cccTAAGGCAACCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTACGATTTCCTCGTTGAAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATAACGGTCGACGTAAGCca       >  1:1264762/1‑150 (MQ=255)
                                                                                                                                                ccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcag      >  1:1174327/1‑150 (MQ=255)
                                                                                                                                                 cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc     <  2:398431/150‑1 (MQ=255)
                                                                                                                                                 cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc     >  1:366732/1‑150 (MQ=255)
                                                                                                                                                 cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc     <  1:455317/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGATCCTGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGA  >  NC_000913/3918151‑3918448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: