Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,917 T→A 7.5% K314I (AAA→ATA) ‡ atpA ← F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,9170TA 7.5% 441.1 / 19.6 161K314I (AAA→ATA) ‡atpAF1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (72/77);  new base A (7/5);  total (79/82)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 167 total aligned reads displayed.

TACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTA  >  NC_000913/3918768‑3919066
                                                                                                                                                     |                                                                                                                                                     
 aCCGGCGTGGAACAGGTTGGATTCCAGGAAGATCTTACCATCGGTAATGGAGATTACGTTGGTCGGAACGATCGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGGCAGAGAACCGGTTTTCCCTTTCACTTCTCCttt                                                                                                                                                      <  2:187700/150‑1 (MQ=255)
   cGGCGTTGAACAGGTTGGTTTCCAGGAAGTTCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTgg                                                                                                                                                    <  2:346387/150‑1 (MQ=255)
    ggCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGt                                                                                                                                                   >  1:1181608/1‑150 (MQ=255)
      cGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGa                                                                                                                                                 <  1:810222/150‑1 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAgg                                                                                                                                              <  1:440125/150‑1 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAgg                                                                                                                                              <  1:994649/150‑1 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAgg                                                                                                                                              <  1:1325194/150‑1 (MQ=255)
           aaCAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCt                                                                                                                                            >  2:1269771/1‑150 (MQ=255)
           aaCAGGTTGGATTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACATCACCTTTGGTGAAGGCt                                                                                                                                            <  1:151606/150‑1 (MQ=255)
              aGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCa                                                                                                                                         <  2:578349/150‑1 (MQ=255)
              aGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgtgaaag                                                                                                                                         <  1:629504/150‑7 (MQ=255)
                gttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGTCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAAc                                                                                                                                       >  2:251941/1‑150 (MQ=255)
                 ttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACg                                                                                                                                      >  2:637015/1‑150 (MQ=255)
                    gttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTAt                                                                                                                                   >  2:200679/1‑150 (MQ=255)
                    gttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgtgaaa                                                                                                                                          >  2:1510614/1‑138 (MQ=255)
                    gttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgtgaaa                                                                                                                                          <  1:1510614/143‑6 (MQ=255)
                     tttCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGtttt                                                                                                                                  <  2:1517069/150‑1 (MQ=255)
                        ccAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCgg                                                                                                                               >  1:542809/1‑150 (MQ=255)
                         cAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGc                                                                                                                              >  1:647552/1‑150 (MQ=255)
                           ggAAGAGCTGCCCATCGGTAATGGAGATTACGTGGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGGGCGGGCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGt                                                                                                                            <  2:97633/150‑1 (MQ=255)
                             aaGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTa                                                                                                                          <  2:1235989/150‑1 (MQ=255)
                             aaGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTa                                                                                                                          <  2:441734/150‑1 (MQ=255)
                               gATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCAGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACAGGTTTTCCCTTTCACTTGACGTTTGGTGAAGGCTTCAACGTATTCGGCGTTAac                                                                                                                        >  1:1258984/1‑150 (MQ=255)
                               gATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGGCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAac                                                                                                                        <  2:720198/150‑1 (MQ=255)
                                  cTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACg                                                                                                                     >  1:213804/1‑150 (MQ=255)
                                   tGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGt                                                                                                                    <  1:1292016/150‑1 (MQ=255)
                                      ccATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcc                                                                                                                 >  1:1060522/1‑149 (MQ=255)
                                      ccATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgca                                                                                                                 <  2:1197538/150‑1 (MQ=255)
                                       cATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTACCACGTgcag                                                                                                                >  2:35305/1‑150 (MQ=255)
                                         tCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGACTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACAGGTTTTCCCATTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcagca                                                                                                              >  2:903365/1‑150 (MQ=255)
                                          cGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAACCGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCAc                                                                                                             <  1:1461711/150‑1 (MQ=255)
                                           ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTAAACACGTGCAGCACg                                                                                                            >  1:577284/1‑150 (MQ=255)
                                           ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGAGCGGGCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCAcc                                                                                                            >  1:738665/1‑149 (MQ=255)
                                              aaTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTc                                                                                                         >  2:1628530/1‑150 (MQ=255)
                                               aTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTcc                                                                                                        <  1:324727/150‑1 (MQ=255)
                                               aTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGGCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTcc                                                                                                        <  1:1283824/150‑1 (MQ=255)
                                                 ggAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcag                                                                                                      <  1:159863/150‑1 (MQ=255)
                                                  gagaTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagc                                                                                                     >  1:1598282/1‑150 (MQ=255)
                                                  ctgaTTACGTTGGTCGGAACGAACGCAGAAAGGTCACCCGGATGAGTTTCGATAATCGGCAGTGCGGGCATAGATCCGGTTTTCCCTTTCACTTCACCTTTGTTGAATGCTTAAACGTATTGGGCGTTTACACGTGCAGCACGCTCcagc                                                                                                     <  1:914442/148‑1 (MQ=255)
                                                         cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACgag                                                                                              <  1:1363674/150‑1 (MQ=255)
                                                         cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACgag                                                                                              <  1:1126178/150‑1 (MQ=255)
                                                          gTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCca                                                                                                       <  1:426906/140‑1 (MQ=255)
                                                          gTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCca                                                                                                       >  2:426906/1‑140 (MQ=255)
                                                              gTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGGTTTCCCTATCACTTCACCTTTGGAGCAGGCTTCAACGTATTCGGCCTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTgg                                                                                         >  2:1344338/1‑150 (MQ=255)
                                                               tCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGa                                                                                        >  1:1192226/1‑150 (MQ=255)
                                                               gcGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGa                                                                                        <  1:200679/149‑1 (MQ=255)
                                                                     cgaacgCAGAAACGTCCCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGa                                                                                  >  2:780411/1‑150 (MQ=255)
                                                                     cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGa                                                                                  >  2:1444578/1‑150 (MQ=255)
                                                                      gaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaa                                                                                 <  1:545927/150‑1 (MQ=255)
                                                                        acgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaaaa                                                                               >  1:1175172/1‑150 (MQ=255)
                                                                         cgCAGAAACGGCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAAc                                                                              <  2:647552/150‑1 (MQ=255)
                                                                           cAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGt                                                                            <  2:30941/150‑1 (MQ=255)
                                                                            aGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTc                                                                           <  2:482954/150‑1 (MQ=255)
                                                                             gAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCg                                                                          <  1:958191/150‑1 (MQ=255)
                                                                                aCGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGccc                                                                       <  2:66702/150‑1 (MQ=255)
                                                                                aCGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGccc                                                                       <  2:723088/150‑1 (MQ=255)
                                                                                    cACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGa                                                                   >  1:1690073/1‑150 (MQ=255)
                                                                                     aCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGaa                                                                  <  2:938513/150‑1 (MQ=255)
                                                                                      cccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAAt                                                                 <  1:1693232/150‑1 (MQ=255)
                                                                                      cccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAAt                                                                 >  1:799566/1‑150 (MQ=255)
                                                                                      cccGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCATCAACGTATTCGGCGTTAACACGTGCAGCAAGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAAt                                                                 >  1:901376/1‑150 (MQ=255)
                                                                                          ccTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTTGTGAAGGCTTCAACGTATTCGGCTTTAACACTTTCAGCACTCTCCAGCAGACGAGAGTGGATGTAGAAAACGTCGCCCGGGAATGCtt                                                             <  1:1067202/150‑1 (MQ=255)
                                                                                           cTGAGGTTCGATAATCGGCAGGGCGGTCAGCGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTc                                                            <  1:917442/150‑1 (MQ=255)
                                                                                               gTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                                        >  1:884297/1‑150 (MQ=255)
                                                                                                     aTAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGc                                                  >  2:868726/1‑150 (MQ=255)
                                                                                                     aTAATCGGCAGGGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGc                                                  <  1:1271271/150‑1 (MQ=255)
                                                                                                      tAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCg                                                 <  2:1175172/150‑1 (MQ=255)
                                                                                                           ggCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                                            >  1:1520912/1‑150 (MQ=255)
                                                                                                                      cAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGCCGGAGcagcag                                 >  2:1065612/1‑150 (MQ=255)
                                                                                                                       agagAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACTTCTCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAgg                                <  1:86973/150‑1 (MQ=255)
                                                                                                                       agagAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAgg                                >  1:114549/1‑150 (MQ=255)
                                                                                                                        gagaACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAggg                               >  1:490350/1‑150 (MQ=255)
                                                                                                                        gagaACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAggg                               <  1:588485/150‑1 (MQ=255)
                                                                                                                           aaCCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGgaga                            <  2:1258984/150‑1 (MQ=255)
                                                                                                                             ccGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATc                          >  2:568458/1‑150 (MQ=255)
                                                                                                                             ccGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATc                          >  1:170579/1‑150 (MQ=255)
                                                                                                                             ccGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATc                          <  1:1492758/150‑1 (MQ=255)
                                                                                                                              cgggtatccctttcccttCACCTTTGGTGAAGGCTTCACCGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCt                         <  1:331283/144‑1 (MQ=255)
                                                                                                                              cGGTTTTCCCTTTCCCTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGGGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCt                         <  2:817432/150‑1 (MQ=255)
                                                                                                                                  tttCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACCCGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACg                     <  1:35305/150‑1 (MQ=255)
                                                                                                                                    tCCCTTTCACTTCACCTTTGGTTAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGATCAGCAGGGAGATCTGACGGt                   <  1:1026106/150‑1 (MQ=255)
                                                                                                                                     cccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCCCGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTa                  >  1:1538225/1‑150 (MQ=255)
                                                                                                                                     cccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTG‑‑GTTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAg                >  2:1422830/1‑150 (MQ=255)
                                                                                                                                      ccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTaa                 >  1:702405/1‑150 (MQ=255)
                                                                                                                                       tgaagccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGCCGGAGCAGCAGGGAGATCTGACGGTAAg                >  2:1538469/7‑150 (MQ=255)
                                                                                                                                        attcacatcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGAAGGGAGATCTGACGGTAAGc               <  2:1055785/149‑1 (MQ=255)
                                                                                                                                         aagccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCa              >  2:589266/5‑150 (MQ=255)
                                                                                                                                          tcacttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTG‑‑GTTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACa           <  1:1422830/150‑1 (MQ=255)
                                                                                                                                           cacttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAAc            <  2:1192226/150‑1 (MQ=255)
                                                                                                                                            ccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAAc            <  1:846666/148‑1 (MQ=255)
                                                                                                                                            ccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACa           >  1:221402/2‑150 (MQ=255)
                                                                                                                                             cttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCACCAg          >  2:846240/1‑150 (MQ=255)
                                                                                                                                               tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc        <  2:1598282/150‑1 (MQ=255)
                                                                                                                                               tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc        >  1:76215/1‑150 (MQ=255)
                                                                                                                                               tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc        >  2:367234/1‑150 (MQ=255)
                                                                                                                                               tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc        <  2:1387600/150‑1 (MQ=255)
                                                                                                                                                cacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCt       >  2:1098244/1‑150 (MQ=255)
                                                                                                                                                   cTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGtt    >  1:1631141/1‑150 (MQ=255)
                                                                                                                                                     ttGGTGAAGGCTTAAACTTATTCGGCGTTAACTCGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTa  <  2:1060522/150‑1 (MQ=255)
                                                                                                                                                     ttGGTGAAGGCTTAAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACTGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTa  <  2:901376/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTTA  >  NC_000913/3918768‑3919066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: