Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,920,809 G→A 6.6% P27L (CCG→CTG)  atpF ← F0 sector of membrane‑bound ATP synthase, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,920,8090GA 6.6% 380.7 / 10.0 121P27L (CCG→CTG) atpFF0 sector of membrane‑bound ATP synthase, subunit b
Reads supporting (aligned to +/- strand):  ref base G (63/50);  new base A (5/3);  total (68/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

Only 100 of 129 total aligned reads displayed.

CGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCG  >  NC_000913/3920660‑3920956
                                                                                                                                                     |                                                                                                                                                   
cGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCGTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcg                                                                                                                                                     >  2:1543744/1‑150 (MQ=255)
cGATGATTACCTGGGCTTCCGCTTTCGCTTTTATCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGGCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcg                                                                                                                                                     <  2:438624/150‑1 (MQ=255)
cGAGGATTACCTGGGCGTCCGCTTTCGCTTTTTTCAGCTGGTCGGGCGCGCGGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTGCTGCGGAAGCAAGGCCGGCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcg                                                                                                                                                     <  2:688677/150‑1 (MQ=255)
 gatgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                                                                                    >  2:802986/1‑150 (MQ=255)
 gatgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGGCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCGAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcgg                                                                                                                                                    <  2:1264500/150‑1 (MQ=255)
  ctgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                                                                   <  2:433004/149‑1 (MQ=255)
  atgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATggcggc                                                                                                                                                   <  1:1368399/150‑1 (MQ=255)
   tgatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGcc                                                                                                                                                  >  2:1631782/1‑150 (MQ=255)
    gatTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCa                                                                                                                                                 <  1:550498/150‑1 (MQ=255)
    ccgatcttGGGCTTCCGCTTTCGCTTTTTTCAGCTGGGCGGTCGCTCTGGCCTTTTCAAGTTAAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCa                                                                                                                                                 <  2:157471/143‑1 (MQ=255)
     tatACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGACGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCGTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTAAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCAt                                                                                                                                                <  2:445584/148‑1 (MQ=255)
     atTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCAt                                                                                                                                                <  2:1176985/150‑1 (MQ=255)
          cTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                                                                                           >  2:969799/1‑150 (MQ=255)
          cTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTa                                                                                                                                           <  1:1143323/150‑1 (MQ=255)
           tGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTAc                                                                                                                                          >  1:1675848/1‑150 (MQ=255)
           tGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTAc                                                                                                                                          >  2:1424876/1‑150 (MQ=255)
           tGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCCGCCATTAATGGCAGCCaagatcgg                                                                                                                                          >  1:157471/1‑143 (MQ=255)
           tGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCCTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTAc                                                                                                                                          <  2:633429/150‑1 (MQ=255)
               cTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCa                                                                                                                                      <  1:542268/150‑1 (MQ=255)
                  ccGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGc                                                                                                                                   <  2:1262124/150‑1 (MQ=255)
                   cgctttcgctttTTTCAGCTGGACGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGca                                                                                                                                  >  1:624002/1‑150 (MQ=255)
                    gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                 >  1:1449547/1‑150 (MQ=255)
                    gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                 >  2:481693/1‑150 (MQ=255)
                    gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                 <  1:921341/150‑1 (MQ=255)
                    gctttcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcag                                                                                                                                 >  2:1351768/1‑150 (MQ=255)
                        tcgctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGcagaaca                                                                                                                             <  1:729510/150‑1 (MQ=255)
                          gctttTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGTCTGCCATTAATGGCGGCCATACGTACTTCATGcagaacaga                                                                                                                           >  1:143260/1‑150 (MQ=255)
                             tttttCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacg                                                                                                                        <  2:881729/150‑1 (MQ=255)
                                 tCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTTCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTTACGTATTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAgaacgaaaa                                                                                                                    >  2:307417/1‑148 (MQ=255)
                                  catcTGGTCGGGCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAg                                                                                                                   <  2:252869/147‑1 (MQ=255)
                                   aGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAgg                                                                                                                  <  2:1012260/150‑1 (MQ=255)
                                     cTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGAc                                                                                                                <  2:1510230/150‑1 (MQ=255)
                                      tGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACa                                                                                                               <  2:216894/150‑1 (MQ=255)
                                        gtcggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACaaa                                                                                                             >  2:207986/1‑150 (MQ=255)
                                          cggtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcg                                                                                                           >  1:1050339/1‑150 (MQ=255)
                                            gtcgCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcgcg                                                                                                         <  2:1170430/150‑1 (MQ=255)
                                               gcgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtg                                                                                                      <  1:1424876/150‑1 (MQ=255)
                                               gcgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtg                                                                                                      >  1:1469323/1‑150 (MQ=255)
                                                cgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtgg                                                                                                     <  2:356384/150‑1 (MQ=255)
                                                cgcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTCCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACCGAACGCACAGGACAAACGCGAtgg                                                                                                     >  2:1171294/1‑150 (MQ=255)
                                                 gcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                                                                                    >  1:1707313/1‑150 (MQ=255)
                                                 gcTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggc                                                                                                    >  1:750005/1‑150 (MQ=255)
                                                     gCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctgg                                                                                                <  2:343225/150‑1 (MQ=255)
                                                      ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAAcgc                                                                                                          <  2:825458/139‑1 (MQ=255)
                                                      ccTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAtggcctggc                                                                                               >  2:1493592/1‑150 (MQ=255)
                                                            caaggtcaacgtCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGa                                                                                         >  2:46707/1‑150 (MQ=255)
                                                                     ggtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGt                                                                                >  1:644268/1‑150 (MQ=255)
                                                                      gtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                                                                               >  2:1486690/1‑150 (MQ=255)
                                                                      gtcCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGtt                                                                               >  2:890470/1‑150 (MQ=255)
                                                                        ccTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTaa                                                                             >  1:537969/1‑150 (MQ=255)
                                                                         cTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCATTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGAGGGCCTGCCCGAGGATTGTTGCGTTAAg                                                                            >  2:656225/1‑150 (MQ=255)
                                                                          ttATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                                                                           <  1:207986/150‑1 (MQ=255)
                                                                          ttATGGGCTAGGTCTGTGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGTACAGGACAATCTCGATGGCCTGGCCGAGGATTGTTGCGTTAAGa                                                                           <  1:167845/150‑1 (MQ=255)
                                                                              gtgCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTca                                                                       >  2:1584235/1‑150 (MQ=255)
                                                                                gCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTcaca                                                                     <  1:727874/150‑1 (MQ=255)
                                                                                  tCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGc                                                                   <  2:1444865/150‑1 (MQ=255)
                                                                                       cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt                                                              <  1:1351768/150‑1 (MQ=255)
                                                                                       cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt                                                              <  2:537969/150‑1 (MQ=255)
                                                                                       cTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAt                                                              >  1:355409/1‑150 (MQ=255)
                                                                                         gCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGATGCCATTAATGCCGGCCATACGTACTTCATGCAGAACAGAACCAACAGGACAACCGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAAtcc                                                            >  2:524138/1‑148 (MQ=255)
                                                                                          cGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGcc                                                           <  2:1337758/150‑1 (MQ=255)
                                                                                           ggAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCct                                                          >  1:1716645/1‑150 (MQ=255)
                                                                                           ggAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCct                                                          >  1:1710193/1‑150 (MQ=255)
                                                                                            gAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCctc                                                         >  1:957600/1‑150 (MQ=255)
                                                                                              aGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa                                                       >  2:1427337/1‑150 (MQ=255)
                                                                                              aGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTa                                                       >  2:721562/1‑150 (MQ=255)
                                                                                                cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt                                                     >  2:737490/1‑150 (MQ=255)
                                                                                                cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTAtt                                                     >  1:1584038/1‑150 (MQ=255)
                                                                                                cAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTAAAGATTCACAGCACAATGCCTCTAtt                                                     >  1:944806/1‑150 (MQ=255)
                                                                                                  aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                                                                              >  1:1015262/1‑123 (MQ=255)
                                                                                                  aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTa                                                                              <  2:1015262/123‑1 (MQ=255)
                                                                                                  aGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTa                                                   <  2:1505288/150‑1 (MQ=255)
                                                                                                   ggCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCATGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAg                                                  >  1:383188/1‑150 (MQ=255)
                                                                                                    gCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGt                                                 <  1:1631782/150‑1 (MQ=255)
                                                                                                     ccGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGtt                                                >  1:644175/1‑150 (MQ=255)
                                                                                                       gTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCTTTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGGTAAGATTCACAGCACAATGCCTCTATTTAGTTaa                                              >  2:1046538/1‑150 (MQ=255)
                                                                                                               tttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGa                                      <  1:149536/150‑1 (MQ=255)
                                                                                                                ttCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGat                                     >  1:501031/1‑150 (MQ=255)
                                                                                                                        gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGctct                             >  2:1709280/1‑150 (MQ=255)
                                                                                                                        gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGctct                             >  1:777099/1‑150 (MQ=255)
                                                                                                                        gACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGctct                             <  2:355409/150‑1 (MQ=255)
                                                                                                                             ttttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCCCAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTGTTtaaa                        >  2:1453915/1‑150 (MQ=255)
                                                                                                                              tttCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGATAACGTTCTGATATTGCTCTTtaaat                       <  2:644268/150‑1 (MQ=255)
                                                                                                                              tacgtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaat                       >  1:1246116/6‑150 (MQ=255)
                                                                                                                                 gtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATTCCTCTATTTAGTTAACGTTCTGATATTGctct                             >  2:661654/3‑141 (MQ=255)
                                                                                                                                 gtaTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGctct                             <  1:661654/139‑1 (MQ=255)
                                                                                                                                 cGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTtaaataaa                    >  1:803139/1‑150 (MQ=255)
                                                                                                                                  taTGGCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATaaaa                   >  2:1313940/2‑150 (MQ=255)
                                                                                                                                  gATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATaaaa                   <  2:1707313/150‑1 (MQ=255)
                                                                                                                                  gATGGCTGCCATTAATGGCGGCCATACGTACTTAATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATaaaa                   <  2:1050339/150‑1 (MQ=255)
                                                                                                                                     ggCCGCCATTAATGGCAGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCa                >  1:1389785/1‑150 (MQ=255)
                                                                                                                                      gCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCaa               >  1:11199/1‑150 (MQ=255)
                                                                                                                                       cTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAc              <  2:1584038/150‑1 (MQ=255)
                                                                                                                                       cTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAAc              <  1:737490/150‑1 (MQ=255)
                                                                                                                                         gCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGc            >  2:1658992/1‑150 (MQ=255)
                                                                                                                                          ccATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCt           <  2:594501/150‑1 (MQ=255)
                                                                                                                                            aTTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTta         <  1:1486690/150‑1 (MQ=255)
                                                                                                                                             ttAATGGCGGCCTTACGTACTTCATGCATAACAGAACGAACAGGACAAACGAGATGGTCTGGCCGAGGATTTTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtac        <  1:296610/150‑1 (MQ=255)
                                                                                                                                              tAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTtact       <  1:1292170/150‑1 (MQ=255)
                                                                                                                                               aaTGGCGGCCATACGTACTACATGCAGAACAGAACGAACAGGACAAACTCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGATAAAGATAAGATATTGATCTTTAAATAAAACCAACGCTaacta      >  1:1225670/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGTGCTCGTTCTGCGGAAGCAAGGCCGTCAGCAATTTCTTTTTGACGTTTTTCGATGGCTGCCATTAATGGCGGCCATACGTACTTCATGCAGAACAGAACGAACAGGACAAACGCGATGGCCTGGCCGAGGATTGTTGCGTTAAGATTCACAGCACAATGCCTCTATTTAGTTAACGTTCTGATATTGCTCTTTAAATAAAAGCAACGCTTACTACGCG  >  NC_000913/3920660‑3920956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: