Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,953,436 C→T 5.9% intergenic (+23/‑42) ilvE → / → ilvD branched‑chain amino acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,953,4360CT 5.9% 430.0 / 9.1 135intergenic (+23/‑42)ilvE/ilvDbranched‑chain amino acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (60/67);  new base T (5/3);  total (65/70)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 146 total aligned reads displayed.

ACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATT  >  NC_000913/3953289‑3953585
                                                                                                                                                   |                                                                                                                                                     
aCGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgcac                                                                                                                                                     >  1:208585/1‑150 (MQ=255)
      ttCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACAGTccc                                                                                                                                               >  1:1202499/1‑150 (MQ=255)
       tCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCa                                                                                                                                              <  2:1541696/150‑1 (MQ=255)
            ttGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAttttt                                                                                                                                         <  2:121531/150‑3 (MQ=255)
            ttGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGTGCGTGCCGTCCCAttttt                                                                                                                                         <  1:1364199/150‑3 (MQ=255)
              gtcGAAGGCCGTTGTGGCCCGGTTACCAAACTCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGCCGAAACCGAAGATAAATGGGGCTGGCTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                                       <  1:1538718/148‑1 (MQ=255)
              ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                                       >  2:128243/1‑150 (MQ=255)
              ggCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACga                                                                                                                                       >  2:575386/1‑150 (MQ=255)
               gCGAAGGACGTTGTGGCCCGGAGACCAAACGCATTCAGCAAGCCTTCTTCGGACTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGCGACGGCACGCACCGTCCCATTTACgag                                                                                                                                      <  1:70863/150‑1 (MQ=255)
                cGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACgaga                                                                                                                                     <  1:1030038/150‑1 (MQ=255)
                    ggCCGGTGTTGCCCTGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGTGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGagacaga                                                                                                                                 <  2:469951/150‑1 (MQ=255)
                     gCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGagacagac                                                                                                                                >  2:733314/1‑150 (MQ=255)
                      ccGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAgacagaca                                                                                                                               >  2:1679076/1‑150 (MQ=255)
                      ccGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAgacagaca                                                                                                                               >  1:341692/1‑150 (MQ=255)
                       cGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGacac                                                                                                                              <  1:128243/150‑1 (MQ=255)
                          tgtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTgg                                                                                                                           >  2:209065/1‑150 (MQ=255)
                           gtgGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTggg                                                                                                                          <  1:997732/150‑1 (MQ=255)
                               cccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGta                                                                                                                      <  2:1557914/150‑1 (MQ=255)
                                ccGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGACTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                                     <  2:367777/150‑1 (MQ=255)
                                acGGTTACCAAACTCATTCAGCAAGACTTCTTCGTCCTCATAAATTGCGAAACCGAAGATAAATGGGGCTGGTGAGATCAAGTTAATCAATAAATACAAAAAATTGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaa                                                                                                                     <  1:272713/149‑1 (MQ=255)
                                   gTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaata                                                                                                                  <  1:488044/150‑1 (MQ=255)
                                     tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                                >  1:16584/1‑150 (MQ=255)
                                     tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                                >  2:563385/1‑150 (MQ=255)
                                     tACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGtaaataaa                                                                                                                >  1:22022/1‑150 (MQ=255)
                                        cAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTTCGAGACAGACACTGGGAGTAAATAAAGTa                                                                                                             >  1:906653/1‑150 (MQ=255)
                                         aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                            >  2:15788/1‑150 (MQ=255)
                                         aaaCGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTAt                                                                                                            >  2:237847/1‑150 (MQ=255)
                                          aaCGCATTAATCAAGAATTCTTCGGCGTCTTCACTGGCGAAACAGAAGATAAATTGTGGTGGTTAGTTCAAGTTACTCAATAAATACAAAAATTGGTACTGCACGCACCGTCCCATTTACTAGACAGACACTGGGAGTAAATAAAGTATg                                                                                                           <  1:143824/150‑1 (MQ=255)
                                            cGCATTCAGCAAGTCTTATTCGGCCTATTCACTGGCGAATCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                                         <  1:523780/150‑1 (MQ=255)
                                            cGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                                         <  2:878086/150‑1 (MQ=255)
                                            cGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACAGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGcc                                                                                                         <  2:1202499/150‑1 (MQ=255)
                                              cATTCAGCAAGCCTTCTTCTGCCTCTTCACTGGCGAAACCGAATATTAATGGGGGTGGTTAGATCAAGTTAATAAATTATTTATAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACCCTGGGTGTAATTAAAGTATGCCTa                                                                                                       <  2:1494880/150‑1 (MQ=255)
                                              cATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTa                                                                                                       >  1:646386/1‑150 (MQ=255)
                                              cATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTa                                                                                                       <  1:333169/150‑1 (MQ=255)
                                                   aGCAAGCCTTCTTCGGCCTCTTCACTGGCTAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                                  <  1:904219/150‑1 (MQ=255)
                                                   aGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                                                                  <  1:733314/150‑1 (MQ=255)
                                                     cAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCg                                                                                                >  1:606683/1‑150 (MQ=255)
                                                       aGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGtt                                                                                              >  2:1409737/1‑150 (MQ=255)
                                                           ttcttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGc                                                                                          <  1:923016/150‑1 (MQ=255)
                                                              ttcGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccac                                                                                       >  1:923276/1‑150 (MQ=255)
                                                                cGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacac                                                                                     >  2:915039/1‑148 (MQ=255)
                                                                  gCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacc                                                                                   >  2:717196/1‑150 (MQ=255)
                                                                    ctctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCcaccaccac                                                                                 <  1:209065/150‑1 (MQ=255)
                                                                     tctTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGCCGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                                                                <  1:143447/150‑1 (MQ=255)
                                                                     tctTAACTGTAGAAAACGAAGATAAATGGGGCTGGTTAGATCAATTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                                                                <  1:148683/150‑1 (MQ=255)
                                                                        tCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCAt                                                                             >  2:58392/1‑150 (MQ=255)
                                                                           cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                                          >  1:1018329/1‑150 (MQ=255)
                                                                           cTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                                                          >  2:953400/1‑150 (MQ=255)
                                                                           cTGGAGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCCCCACCACTCTTggg                                                                          >  2:817736/1‑149 (MQ=255)
                                                                             ggCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCg                                                                                           >  2:444903/1‑131 (MQ=255)
                                                                             ggCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCg                                                                                           <  1:444903/131‑1 (MQ=255)
                                                                             ggCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCg                                                                        >  1:1195833/1‑150 (MQ=255)
                                                                               cGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTa                                                                      >  1:1711184/1‑150 (MQ=255)
                                                                                     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                                                                >  2:842012/1‑150 (MQ=255)
                                                                                       caGATACACGGGGCTGGCTAGAACAAGATAATCAATAAAACCACAAAAATGGACGGCACTCCCCGTCCCATTTACGAGACAGCCACTTGCAGCAAATAAAGTAAGCCTAAGTACCGTTCCGCCACCACCACTCATGGGCGAAATATGGCg                                                              <  2:340958/149‑1 (MQ=255)
                                                                                       aaGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCg                                                              <  2:6211/150‑1 (MQ=255)
                                                                                        aGATAAATGGGGCTGGTTAGATCAAGTTAATCAATCAATACAAAAAATGGGACGGCACGCACCTTCCCATTTACGAGACAGACACTGTGAGTAAATAAAGTATGCCTAAGTTCCGTTCCGCCACCACCACTCATGGTCGTATATGGCggg                                                            <  2:1058527/150‑1 (MQ=255)
                                                                                        aGATAAATGGGGCTGGTTACATCAAGTTAAACAATAAATACAAAAAATGCGAAGTCACGCACCGTCCCATTTACTATACAGACACTGGGAGTAAAAAAAGTATGACTAAGTACCGTTCCGCCACCACCACTCATTGACGTAATATggatg                                                             >  2:548490/1‑147 (MQ=255)
                                                                                         taTAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                                                            <  2:923276/149‑1 (MQ=255)
                                                                                         gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                                                            <  2:752332/150‑1 (MQ=255)
                                                                                          aTAAATGGGGCTTGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGt                                                           <  2:1108524/150‑1 (MQ=255)
                                                                                           aaaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTg                                                          >  1:1590821/2‑150 (MQ=255)
                                                                                             aaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCt                                                        >  2:381293/1‑150 (MQ=255)
                                                                                                    cTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGGCGTAATATGGCGGGTGCTCGTgcgc                                                 <  1:222051/150‑1 (MQ=255)
                                                                                                     tGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCt                                                >  2:1664912/1‑150 (MQ=255)
                                                                                                      ggTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtg                                               <  2:545325/150‑1 (MQ=255)
                                                                                                       gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCtgt                                              <  1:9783/150‑1 (MQ=255)
                                                                                                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCTTGCGCtgtg                                             <  1:1716224/150‑1 (MQ=255)
                                                                                                          aGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgc                                           >  2:1303451/1‑150 (MQ=255)
                                                                                                           gATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCCCCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcg                                          >  1:1048651/1‑150 (MQ=255)
                                                                                                            aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgc                                         >  2:603211/1‑150 (MQ=255)
                                                                                                             tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcg                                        >  1:438045/1‑150 (MQ=255)
                                                                                                             tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGgcgcg                                        <  2:1018329/150‑1 (MQ=255)
                                                                                                                  atAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCAcc                                   <  1:1259538/149‑1 (MQ=255)
                                                                                                                    aaTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCgg                                 <  1:717196/150‑1 (MQ=255)
                                                                                                                       cAATAAATACAAAAAATGGGACGGCACGCACCGTCTCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAAt                              <  2:208585/150‑1 (MQ=255)
                                                                                                                        aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATg                             >  2:956744/1‑150 (MQ=255)
                                                                                                                        aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCGAAGTACCGTTCCGCCACAACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGAGAGCCACCGGGATg                             >  2:1027278/1‑150 (MQ=255)
                                                                                                                        aataaataCAAAAAATGGGACGGCACGCACAGTCCCATTTACTAGACAGAAAATTGGAGTAAATAAAGTATGCCTAAGTACAGTTACGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTTTGGCGCGCCCCCGGAATg                             >  2:444573/1‑150 (MQ=255)
                                                                                                                           aaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc                          <  1:237847/150‑1 (MQ=255)
                                                                                                                           aaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGAcc                          <  1:1514520/150‑1 (MQ=255)
                                                                                                                             ataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGa                        <  1:1679076/150‑1 (MQ=255)
                                                                                                                                cAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGc                     >  2:1441366/1‑150 (MQ=255)
                                                                                                                                 aaaaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGcc                    <  2:1711184/150‑1 (MQ=255)
                                                                                                                                 aaaaaaCGGGACGGCACGCACCGTCCCATTTACGAGACAGACACGGGGAGTAAATAATGTATGCCTAAGTACCGTTCCGCCCCCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGATTGACCGACGcc                    >  1:815761/1‑150 (MQ=255)
                                                                                                                                   taaaTGGGACGGTGCGTGCCGTCGCATTTACGAGACAGACACTGGGAGTAATTAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATCGTCGTAATTAGGCGAGTGCTCGTGCGCTGAGGCGCGCCACCGGAATGACCGCCGCCGa                  >  1:290656/2‑150 (MQ=255)
                                                                                                                                   taaaTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGa                  >  1:830534/2‑150 (MQ=255)
                                                                                                                                    aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACAACTCATGGTCGTAATATGGCGCGTGCTCGTGCGCTTTGGCGCGCCACCGGAATGACCGACGCCGAt                 >  2:288737/1‑150 (MQ=255)
                                                                                                                                      aTGGGACGGTGCGTGCCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAttt               >  2:1473927/1‑150 (MQ=255)
                                                                                                                                      aTGGGACGGCACGCACCGTCCCAGTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGAgtt               >  2:325698/1‑150 (MQ=255)
                                                                                                                                       tGGGACGGTGCGTGCCGTGCCATTTACGAGACGGACACTGGGAGTAAATAAAGTATGCCTAAGTACCTTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCGCCGGAATGCCCGCCGCCGATTTc              >  2:294536/1‑150 (MQ=255)
                                                                                                                                        gggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCg             <  2:241283/150‑1 (MQ=255)
                                                                                                                                        gggACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCg             <  2:490614/150‑1 (MQ=255)
                                                                                                                                               cacgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCCCCGGAATGACCGACGCCGATTTCGGTAAGcc      >  1:1377296/1‑150 (MQ=255)
                                                                                                                                               cacgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGcc      >  2:1042965/1‑150 (MQ=255)
                                                                                                                                               cacgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCCCCACCGGAATGACCGACGCCGATTTCGGTAAGcc      >  2:326706/1‑150 (MQ=255)
                                                                                                                                                 cgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGa    <  1:58392/150‑1 (MQ=255)
                                                                                                                                                 cgcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGa    <  2:16584/150‑1 (MQ=255)
                                                                                                                                                  gcacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGAt   <  1:1678324/150‑1 (MQ=255)
                                                                                                                                                   cacCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGAtt  <  1:575386/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGCGCGCCACCGGAATGACCGACGCCGATTTCGGTAAGCCGATT  >  NC_000913/3953289‑3953585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: