Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,926 A→T 8.6% intergenic (‑113/+86) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,9260AT 8.6% 365.5 / 19.4 140intergenic (‑113/+86)ppiC/yifNpeptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
Reads supporting (aligned to +/- strand):  ref base A (63/65);  new base T (8/4);  total (71/69)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 145 total aligned reads displayed.

TACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAAC  >  NC_000913/3959778‑3960075
                                                                                                                                                    |                                                                                                                                                     
taaaaGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAGTAATTGTCAGGCGGCGATTTTAGAATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAt                                                                                                                                                      >  1:527981/4‑150 (MQ=255)
 aCAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAACAGCCCGGAAAATc                                                                                                                                                     >  2:268630/1‑150 (MQ=255)
  cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                    >  2:865231/1‑150 (MQ=255)
  cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                    <  2:714804/150‑1 (MQ=255)
  cAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATcc                                                                                                                                                    >  1:938221/1‑150 (MQ=255)
   aaGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCg                                                                                                                                                   <  2:899975/150‑1 (MQ=255)
      gATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGc                                                                                                                                                <  2:370045/150‑1 (MQ=255)
           tcAGTGGGGCTGCTGTTTTTGCCATGATCGGTCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                                                                           <  2:158826/149‑4 (MQ=255)
           gCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGc                                                                                                                                           >  1:1101029/1‑150 (MQ=255)
           gCAGTGCGTCTGCTGTTTTTTCCATGATCGTGCCTTGAGTGAATATTTGTCAGGCTGATATTTTAGCATTATCCTCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                                                                           <  2:6537/150‑4 (MQ=255)
            cAGTGCTGCTGCTGTTTTTCCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGAGTTTAGCATGATCCGCAGATGCTAACGTATTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCa                                                                                                                                          <  1:439132/150‑1 (MQ=255)
              gTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTTTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAgg                                                                                                                                        <  1:313080/150‑1 (MQ=255)
               tgctgctgcTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGt                                                                                                                                       >  1:1294789/1‑150 (MQ=255)
                  tgctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATTATCCGCAGATGCTTACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTacc                                                                                                                                    >  2:908304/1‑150 (MQ=255)
                  tgctgctgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTccc                                                                                                                                    >  2:378391/1‑150 (MQ=255)
                        tgTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgg                                                                                                                              <  2:1499701/150‑1 (MQ=255)
                         gTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAggg                                                                                                                             <  2:1212363/150‑1 (MQ=255)
                           ttttGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGga                                                                                                                           >  1:977629/1‑150 (MQ=255)
                             ttGCCATGATCGTGCCTTGAGTGATTTATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAATGGgaga                                                                                                                         <  2:527981/150‑1 (MQ=255)
                              tGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaa                                                                                                                        >  1:460241/1‑150 (MQ=255)
                               gCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaa                                                                                                                       >  1:1107859/1‑150 (MQ=255)
                                ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  1:417604/1‑150 (MQ=255)
                                ccATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaaa                                                                                                                      >  1:1162310/1‑150 (MQ=255)
                                 cATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCATATGCTAACGTAATTGTCAGTCAGGGAGTGTCTGGTACATATATGGCAAAAGCCCGGAAAAACCGGGCTTCGCAGGTCCCTCAAGTGGAGaaaaa                                                                                                                     >  1:650456/1‑149 (MQ=255)
                                 cATGATCGGTCCTTTAGTGTATAATTGTCAGGAGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                                                                     <  2:1433126/150‑1 (MQ=255)
                                    gATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                                                                                  <  2:810547/150‑1 (MQ=255)
                                     aTCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAACCTTAg                                                                                                                 >  1:1520412/1‑150 (MQ=255)
                                       cGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAggg                                                                                                               <  2:1002449/150‑1 (MQ=255)
                                       cGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGGCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAggg                                                                                                               <  2:1015881/150‑1 (MQ=255)
                                        gTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGc                                                                                                              >  2:397913/1‑150 (MQ=255)
                                         tGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGATTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                                                             >  1:88045/1‑150 (MQ=255)
                                         tGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                                                                             <  2:498761/150‑1 (MQ=255)
                                           ccTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                                                                           >  1:849955/1‑150 (MQ=255)
                                                   gAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                                                                   <  1:1265224/150‑1 (MQ=255)
                                                      taatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                                                                <  1:1404620/150‑1 (MQ=255)
                                                       aatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                                                                               <  1:612850/150‑1 (MQ=255)
                                                       aatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCAGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                                                                               >  2:334892/1‑150 (MQ=255)
                                                          tGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGATTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                                                                            <  2:88045/150‑1 (MQ=255)
                                                                     gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                                                                 >  1:648664/1‑150 (MQ=255)
                                                                     gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                                                                 >  1:546286/1‑150 (MQ=255)
                                                                       ttttAGGATGATACTCAGATTCTTACGTTATTGTGAGTCTGGGATTGTATTGTACATATATGGCAAATGAACTGATAATCCGGGGTTCGCATGTCACTCAAGGGGAGAAAACTTAGGGCCTCTATGTGCTACAAAGTCTTTATCGTGGAt                                                                               <  2:650456/150‑1 (MQ=255)
                                                                          tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                                                                            >  1:1350585/1‑150 (MQ=255)
                                                                           aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtt                                                                           >  1:1456189/1‑150 (MQ=255)
                                                                            gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                                                                          >  1:1377060/1‑150 (MQ=255)
                                                                            gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                                                                          <  2:1395689/150‑1 (MQ=255)
                                                                               tGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                                                                       <  2:604790/150‑1 (MQ=255)
                                                                               tGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                                                                            >  2:1329311/1‑145 (MQ=255)
                                                                                gATCCGCAGATGCTAACGTAATTTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                                                                     >  2:1605128/1‑150 (MQ=255)
                                                                                            cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGTGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTa                                                          <  2:1234464/150‑1 (MQ=255)
                                                                                            cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTa                                                          <  2:21231/150‑1 (MQ=255)
                                                                                            cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTa                                                          <  1:1385327/150‑1 (MQ=255)
                                                                                            atAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCTGGGATGGTTATTTTATTGTCAATTTa                                                          <  2:1325080/149‑1 (MQ=255)
                                                                                              aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATg                                                        >  1:1021262/1‑150 (MQ=255)
                                                                                              aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATg                                                        <  2:417604/150‑1 (MQ=255)
                                                                                              aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATg                                                        <  2:648664/150‑1 (MQ=255)
                                                                                                cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg                                                      <  2:1477276/150‑1 (MQ=255)
                                                                                                 gTAATTTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg                                                    <  1:1605128/150‑1 (MQ=255)
                                                                                                  tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg                                                    >  2:809933/1‑150 (MQ=255)
                                                                                                     ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata                                                 <  2:1588981/150‑1 (MQ=255)
                                                                                                      tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat                                                <  1:1385924/150‑1 (MQ=255)
                                                                                                         cagtcagGGAGTGGCTGGTACATATATGTCAAAAGCCCGGAAAATCCGGGCTTCGCAGTACCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGc                                             <  1:908304/150‑1 (MQ=255)
                                                                                                         cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGc                                             >  1:766044/1‑150 (MQ=255)
                                                                                                         cagtcagGGAGTGGCGTGTACATATATCGCAAAAGCCCGGAAAATCAGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGc                                             <  2:1101029/150‑1 (MQ=255)
                                                                                                             cagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCattg                                         <  2:1559763/150‑1 (MQ=255)
                                                                                                                   gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGAc                                   <  2:113701/150‑1 (MQ=255)
                                                                                                                   gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGAc                                   <  1:814880/150‑1 (MQ=255)
                                                                                                                   gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGAc                                   <  2:76798/150‑1 (MQ=255)
                                                                                                                    tGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACt                                  >  1:1712056/1‑150 (MQ=255)
                                                                                                                       cTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga                               >  2:1253558/1‑150 (MQ=255)
                                                                                                                       cTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga                               >  1:500032/1‑150 (MQ=255)
                                                                                                                       cTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGGCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtga                               <  1:617263/150‑1 (MQ=255)
                                                                                                                         ggTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTtgatg                             <  2:1587257/150‑1 (MQ=255)
                                                                                                                         ggTACATAAATCGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGACTCTATGGGCAACAAAGTCATTATCGGGGTTGATTATTTTTTTGTAAATTTATGAGCGATATGTCAATCATTGACTtgatg                             >  2:142134/1‑150 (MQ=255)
                                                                                                                           tACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGAc                           >  1:161730/1‑150 (MQ=255)
                                                                                                                            aCATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGAcc                          >  1:1488615/1‑150 (MQ=255)
                                                                                                                             cATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCg                         <  2:1517117/150‑1 (MQ=255)
                                                                                                                             cATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAAGGGCTACAAAGGCATAATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCg                         >  1:977639/1‑150 (MQ=255)
                                                                                                                               tatatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGTAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCTcc                       >  1:458700/1‑150 (MQ=255)
                                                                                                                               tatatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGcc                       >  2:1153663/1‑150 (MQ=255)
                                                                                                                                 tatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCATCTATGGGCTACAAAGTCATTATCGGGGATGGTTAGTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGccat                     >  1:938787/1‑148 (MQ=255)
                                                                                                                                   tGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTAAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCctct                   <  1:1502290/150‑1 (MQ=255)
                                                                                                                                   cctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATTTGGCATTGATTGACTTGATGACCGCCctct                   >  2:1348540/7‑150 (MQ=255)
                                                                                                                                   cctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCctct                   >  1:1537047/7‑150 (MQ=255)
                                                                                                                                    ctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCTCCCTCTg                  >  1:259664/6‑150 (MQ=255)
                                                                                                                                    ctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTg                  >  2:1197187/6‑150 (MQ=255)
                                                                                                                                      gcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa                <  2:259664/147‑1 (MQ=255)
                                                                                                                                      cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCCTTATCGGGGATGCTTATGTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa                >  1:890253/1‑150 (MQ=255)
                                                                                                                                      cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTa                >  1:1307830/1‑150 (MQ=255)
                                                                                                                                      cAAAAGCCCGGAAAATCCGGGCTACGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATCGGCTACAAAGCCATGATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCGCTGtt                >  1:75343/1‑149 (MQ=255)
                                                                                                                                       cgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTAt               >  2:355831/3‑150 (MQ=255)
                                                                                                                                        aaaGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATg              >  2:177478/1‑150 (MQ=255)
                                                                                                                                         aaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCTTCTGTATGt             >  2:371799/1‑150 (MQ=255)
                                                                                                                                         aaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGt             >  1:290930/1‑150 (MQ=255)
                                                                                                                                          aGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTc            <  2:1162310/150‑1 (MQ=255)
                                                                                                                                            cccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGGCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTg          <  2:59798/150‑1 (MQ=255)
                                                                                                                                            cccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTTTATGTCTg          >  1:1429176/1‑150 (MQ=255)
                                                                                                                                             ccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGc         >  2:1632761/1‑150 (MQ=255)
                                                                                                                                             ccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGc         <  2:1456189/150‑1 (MQ=255)
                                                                                                                                                 aaaaTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaacc     <  1:378391/150‑1 (MQ=255)
                                                                                                                                                  aaaTCCGGGCTTCGCAGGTCCCTCAAGGTGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGcaacca    <  2:1104264/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGATTGACTTGATGACCGCCCTCTGTATGTCTGCAACCAAC  >  NC_000913/3959778‑3960075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: