Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,008,408 A→G 7.3% intergenic (+31/+31) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4080AG 7.3% 354.8 / 14.4 123intergenic (+31/+31)rhtC/rhtBthreonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
Reads supporting (aligned to +/- strand):  ref base A (60/54);  new base G (5/4);  total (65/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

Only 100 of 124 total aligned reads displayed.

CCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCA  >  NC_000913/4008259‑4008556
                                                                                                                                                     |                                                                                                                                                    
ccAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATa                                                                                                                                                      <  1:1404099/150‑1 (MQ=255)
   gCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAgg                                                                                                                                                   <  1:551663/150‑1 (MQ=255)
    ccTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGc                                                                                                                                                  >  2:1115462/1‑150 (MQ=255)
     cTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCg                                                                                                                                                 <  1:764206/150‑1 (MQ=255)
      tGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGAAAGTCGGATAAGGctt                                                                                                                                                >  1:1054783/1‑148 (MQ=255)
       gTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGtt                                                                                                                                               >  2:1338733/1‑150 (MQ=255)
        ttttCCCTGCGGCAAATGGGCCGTGGTTATCAACGTCTTGCGAATTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCAGTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGttt                                                                                                                                              <  1:1511504/150‑1 (MQ=255)
          tGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAc                                                                                                                                            >  2:509934/1‑150 (MQ=255)
           gCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAcg                                                                                                                                           >  2:1605905/1‑150 (MQ=255)
            cccTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAcgc                                                                                                                                          <  1:411389/150‑1 (MQ=255)
            cccTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAcgc                                                                                                                                          >  2:140433/1‑150 (MQ=255)
            cccTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAcgc                                                                                                                                          >  1:893836/1‑150 (MQ=255)
             ccTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTAcgcc                                                                                                                                         >  2:805781/1‑150 (MQ=255)
                gCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCa                                                                                                                                      >  1:373000/1‑150 (MQ=255)
                  cGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAAcgccttat                                                                                                                                     <  2:651719/149‑5 (MQ=255)
                  cGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATc                                                                                                                                    >  2:12177/1‑150 (MQ=255)
                   gCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                   <  1:1128233/150‑1 (MQ=255)
                    cAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                  >  1:569688/1‑150 (MQ=255)
                     aaaTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGa                                                                                                                                 >  1:1366292/1‑150 (MQ=255)
                      aaTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                <  2:965058/150‑1 (MQ=255)
                       aTGCGCCGTGGTTTTCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATGCGGCGTAAACGCCTTATCCGAct                                                                                                                               <  1:847153/150‑2 (MQ=255)
                       aTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACa                                                                                                                               <  1:290613/150‑1 (MQ=255)
                          cgcCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACatta                                                                                                                            >  2:199291/1‑150 (MQ=255)
                          cgcCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACatta                                                                                                                            >  1:1358656/1‑150 (MQ=255)
                           gcCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACattat                                                                                                                           <  2:250403/150‑1 (MQ=255)
                              gTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTAtttt                                                                                                                        <  1:1277670/150‑1 (MQ=255)
                                ggTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTc                                                                                                                      >  1:1260416/1‑150 (MQ=255)
                                   tATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGCAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACg                                                                                                                   <  1:1098695/150‑1 (MQ=255)
                                     tCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCa                                                                                                                 <  2:1242403/150‑1 (MQ=255)
                                            cTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCg                                                                                                          <  2:367741/150‑1 (MQ=255)
                                              ggCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGcc                                                                                                        >  2:617584/1‑150 (MQ=255)
                                               gCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCg                                                                                                       >  2:1511445/1‑150 (MQ=255)
                                                  aaGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATg                                                                                                    <  1:1605905/150‑1 (MQ=255)
                                                   aGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGc                                                                                                   >  1:1360116/1‑150 (MQ=255)
                                                     tGGTTTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTa                                                                                                 <  1:530152/150‑1 (MQ=255)
                                                       gATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAAc                                                                                               <  1:1371074/150‑1 (MQ=255)
                                                         ttGATGGTTTTGCCGTTGCGTTATTTTCCGGATTTGGCATTGATTTGATTTTTTCGCGGTGATGCCAGACGCGTCTTCTGAGTAAGTCGGATAAGGGGTTTACGCCGCATACGACATTATTTTTCTCGCATGCCTCGCCGATGCTAACAg                                                                                             <  2:1096683/150‑1 (MQ=255)
                                                          tGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgc                                                                                            <  1:509934/150‑1 (MQ=255)
                                                           gATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTAGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGTCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAgcg                                                                                           >  1:217457/1‑150 (MQ=255)
                                                              ggTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTc                                                                                        <  2:935845/150‑1 (MQ=255)
                                                                 tttGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCcca                                                                                     >  2:121366/1‑150 (MQ=255)
                                                                   tGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGGCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCccacc                                                                                   <  2:764821/150‑1 (MQ=255)
                                                                      cGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGc                                                                                <  1:160538/150‑1 (MQ=255)
                                                                         ggCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATa                                                                             <  1:810739/150‑1 (MQ=255)
                                                                          gCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATaa                                                                            >  1:540979/1‑150 (MQ=255)
                                                                           cGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATaaa                                                                           >  2:1672764/1‑150 (MQ=255)
                                                                            gTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaac                                                                          >  1:493634/1‑150 (MQ=255)
                                                                            gTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaac                                                                          <  1:1329505/150‑1 (MQ=255)
                                                                             ttATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaaca                                                                         <  2:1358656/150‑1 (MQ=255)
                                                                              tATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaa                                                                        <  1:418908/150‑1 (MQ=255)
                                                                               aTTTTCCGGATTGTGCATTCATTTGTTTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGTATACGGCGTTTACGCCGCATCCGACATTATTTTTCAAGCATGCCTCGCCGATGCTAACAGCGCTCCCACAAGCATAaacaac                                                                       <  2:1617300/150‑1 (MQ=255)
                                                                               aTTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAaacaac                                                                       <  2:541141/150‑1 (MQ=255)
                                                                                   gCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGc                                                                   <  2:1260416/150‑1 (MQ=255)
                                                                                   gCCGGATTTGGCATTCATTTGATTATTTCGCGGGGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGc                                                                   <  2:552824/150‑1 (MQ=255)
                                                                                    ccGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGcc                                                                  >  1:827212/1‑150 (MQ=255)
                                                                                     cGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCg                                                                 >  1:368817/1‑150 (MQ=255)
                                                                                          ttGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAAt                                                            >  2:818278/1‑150 (MQ=255)
                                                                                           tGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATc                                                           >  2:1544712/1‑150 (MQ=255)
                                                                                             gCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGTCATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCtt                                                         >  1:1471884/1‑150 (MQ=255)
                                                                                             gCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCtt                                                         >  1:1219959/1‑150 (MQ=255)
                                                                                             gCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCtt                                                         >  1:535185/1‑150 (MQ=255)
                                                                                              cattcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                        >  1:786025/1‑150 (MQ=255)
                                                                                              cattcattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGAATCCGACATTATTTTTCACGCTTGCCTCGCCGCTGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTa                                                        >  1:829720/1‑150 (MQ=255)
                                                                                                   ttttGATTCTTTCTGGGGTATGCCAGACGCGTCTACATAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGAGATTATTTTTCACGCATTCCGCGCCGCTGCTAACAGCGCGCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                   <  1:1154383/149‑1 (MQ=255)
                                                                                                   attTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                   >  1:1065445/1‑150 (MQ=255)
                                                                                                   attTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                   <  1:140433/150‑1 (MQ=255)
                                                                                                   attTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGACGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAg                                                   <  2:893836/150‑1 (MQ=255)
                                                                                                     ttGATTATTTCGCTGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAATGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAACCGAAAATCTTATTCAgcg                                                 <  1:944575/150‑1 (MQ=255)
                                                                                                      tGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGACGAAAATCTTATTCAgcgc                                                >  2:298700/1‑150 (MQ=255)
                                                                                                       gATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCTCCATCATAAACAACGAGCCGAAAATCTTATTCAgcgac                                               >  1:89117/1‑148 (MQ=255)
                                                                                                         ttattTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCtt                                             >  2:705125/1‑150 (MQ=255)
                                                                                                          tattTCGCGGTGATGCCAGACGAGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAacgcatcc                                            <  1:199291/150‑5 (MQ=255)
                                                                                                              tCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCt                                        <  1:1511445/150‑1 (MQ=255)
                                                                                                                    ggATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTg                                  <  2:103647/149‑1 (MQ=255)
                                                                                                                      aTGCCAGACGCTTCTTCAGAGTAAGTAGGATAAGGCGTTTACGCCGCAGCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGt                                <  2:1054783/150‑1 (MQ=255)
                                                                                                                      aTGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGt                                >  1:291175/1‑150 (MQ=255)
                                                                                                                        gCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTcc                              >  2:1602882/1‑150 (MQ=255)
                                                                                                                           aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCttt                           >  1:832867/1‑150 (MQ=255)
                                                                                                                           aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCttt                           <  2:368817/150‑1 (MQ=255)
                                                                                                                            gACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTa                          <  1:1648694/150‑1 (MQ=255)
                                                                                                                             aCGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTaa                         <  2:1366292/150‑1 (MQ=255)
                                                                                                                               gcgTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATc                       <  1:774892/150‑1 (MQ=255)
                                                                                                                               gcgGCTTCAGAGTAAGTCGGATAATGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACATCGCTCCCACCATCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTTGTCCTTTAATc                       <  2:829720/150‑1 (MQ=255)
                                                                                                                                cgTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACACTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATcc                      >  2:456122/1‑150 (MQ=255)
                                                                                                                                  tCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCAt                    >  2:820829/1‑150 (MQ=255)
                                                                                                                                  gcTTCAGAGTAAGTCGGATACGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCAt                    <  2:1631988/149‑1 (MQ=255)
                                                                                                                                   cTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGACTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCGTCATCTGCTTTGGTCGTTTAATCCATa                   >  2:300077/1‑150 (MQ=255)
                                                                                                                                     tCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATaga                 >  2:150485/1‑150 (MQ=255)
                                                                                                                                       agagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGc               <  1:1672764/150‑1 (MQ=255)
                                                                                                                                        gagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCa              >  1:484042/1‑150 (MQ=255)
                                                                                                                                        gagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCa              >  1:906717/1‑150 (MQ=255)
                                                                                                                                        aataatgTCGGATGCGGCGTAAACGCCTTATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCa              >  2:177037/7‑150 (MQ=38)
                                                                                                                                            aaGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATcc          >  1:158674/1‑150 (MQ=255)
                                                                                                                                              gTCGGATGCGGCGTAAACGCCTTATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGt        >  1:914135/1‑150 (MQ=255)
                                                                                                                                               tCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGtt       >  1:295024/1‑150 (MQ=255)
                                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTg      >  2:1544680/1‑150 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGa     <  1:1434063/150‑1 (MQ=255)
                                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAg    >  2:891512/1‑150 (MQ=255)
                                                                                                                                                    tAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCa  <  1:1544712/150‑1 (MQ=255)
                                                                                                                                                    tAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCa  >  2:185260/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCA  >  NC_000913/4008259‑4008556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: