Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,028,482 T→G 5.1% A97A (GCA→GCC fadA ← 3‑ketoacyl‑CoA thiolase (thiolase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,028,4820TG 5.1% 316.1 / 2.4 104A97A (GCA→GCCfadA3‑ketoacyl‑CoA thiolase (thiolase I)
Reads supporting (aligned to +/- strand):  ref base T (47/50);  new base G (4/2);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAG  >  NC_000913/4028334‑4028625
                                                                                                                                                    |                                                                                                                                               
tcatGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTg                                                                                                                                                >  1:1172952/1‑150 (MQ=255)
tcatGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGATGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTg                                                                                                                                                <  1:782471/150‑1 (MQ=255)
  ctGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGcc                                                                                                                                              >  1:1160810/2‑150 (MQ=255)
  atGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGcc                                                                                                                                              <  2:1377944/150‑1 (MQ=255)
   tGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGCTCATTCGTGCTGCGTCATGCAGTGCCt                                                                                                                                             >  2:328577/1‑150 (MQ=255)
     cccGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGc                                                                                                                                           >  1:432722/1‑150 (MQ=255)
     cccGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGc                                                                                                                                           >  1:1577205/1‑150 (MQ=255)
     cccGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGc                                                                                                                                           >  2:1330918/1‑150 (MQ=255)
      ccgccgCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCa                                                                                                                                          <  1:842412/150‑1 (MQ=255)
      ccgccgCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCa                                                                                                                                          >  1:714581/1‑150 (MQ=255)
      ccgccgCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCAGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCa                                                                                                                                          >  2:1677672/1‑150 (MQ=255)
        gccgcTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATg                                                                                                                                        >  1:729404/1‑150 (MQ=255)
          cgcTTTGGCGACATTGCGGCTCAGGCCGGGGGGAAACTCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGGCATGCAGGCACTGCATgac                                                                                                                                      <  1:1072771/150‑3 (MQ=255)
           gcTTTGGCGACATTGCGGCTCAGGCCGGGGGGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATgacg                                                                                                                                     <  2:42166/150‑4 (MQ=255)
            cTTTGTCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACTTGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCTCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATg                                                                                                                                    <  1:1211366/150‑1 (MQ=255)
            cTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATg                                                                                                                                    <  2:787485/150‑1 (MQ=255)
                ggCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAAcc                                                                                                                                <  1:826243/150‑1 (MQ=255)
                     cATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGTCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACcacaca                                                                                                                           <  2:48471/150‑1 (MQ=255)
                     cATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACcacaca                                                                                                                           >  2:1439713/1‑150 (MQ=255)
                     cATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACcacaca                                                                                                                           <  2:468414/150‑1 (MQ=255)
                        tGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGc                                                                                                                        >  2:1031130/1‑150 (MQ=255)
                        tGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGc                                                                                                                        >  2:404694/1‑150 (MQ=255)
                           ggCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGAt                                                                                                                     <  2:730707/150‑1 (MQ=255)
                             cTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTa                                                                                                                   <  2:1172952/150‑1 (MQ=255)
                              tCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTaa                                                                                                                  <  1:480242/150‑1 (MQ=255)
                               cAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAAc                                                                                                                 <  2:81187/150‑1 (MQ=255)
                                aGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                <  2:1203655/150‑1 (MQ=255)
                                  gCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCAGGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACTGt                                                                                                              >  2:1492795/1‑150 (MQ=255)
                                     ggggTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAc                                                                                                           <  2:107079/150‑1 (MQ=255)
                                       ggTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAccg                                                                                                         >  1:1118687/1‑150 (MQ=255)
                                       ggTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAccg                                                                                                         <  1:1439713/150‑1 (MQ=255)
                                         tGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGACCCACACCAGCGATTAACGCTAAccggc                                                                                                       >  2:1169388/1‑148 (MQ=255)
                                         tGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAccgcc                                                                                                       >  1:1661964/1‑150 (MQ=255)
                                            aaaTCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCggg                                                                                                    <  2:1661964/150‑1 (MQ=255)
                                              aTCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAc                                                                                                  >  1:528673/1‑150 (MQ=255)
                                              aTCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAc                                                                                                  <  1:1478262/150‑1 (MQ=255)
                                                  aCGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACagag                                                                                              <  2:140838/150‑1 (MQ=255)
                                                    gCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtg                                                                                            >  1:531138/1‑150 (MQ=255)
                                                    gCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtg                                                                                            >  2:1292675/1‑150 (MQ=255)
                                                     ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtgt                                                                                           >  1:1656951/1‑150 (MQ=255)
                                                     ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtgt                                                                                           >  1:1612144/1‑150 (MQ=255)
                                                     ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtgt                                                                                           <  2:714581/150‑1 (MQ=255)
                                                     ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATAATTCCTGCTGCGTCATGCAGTGCCTGCATGCATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgagt                                                                                           >  2:206668/1‑150 (MQ=255)
                                                        ggACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGt                                                                                        <  2:1226803/149‑1 (MQ=255)
                                                           cTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTTCt                                                                                     >  1:403150/1‑150 (MQ=255)
                                                           cTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACt                                                                                     >  2:1345255/1‑150 (MQ=255)
                                                           cTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACt                                                                                     >  2:1400218/1‑150 (MQ=255)
                                                              aTCGGCACATTGCCCATATGCTACACGCCGCCAACGAGACATGCCTGGGCATCGCGAGTCATGATCATTCGTGCTGCGTCAAGCAGAGCCTGCATGGATGAACCACACAAGCGATTAACGGTTACCGCCGGTACAGAGTGTGGTACTTCt                                                                                  <  1:664051/150‑1 (MQ=255)
                                                              aTCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                  >  1:790603/1‑150 (MQ=255)
                                                               tCGGCACATGGCCCATATGCTCCTCGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGTTACTTCTg                                                                                 <  1:1197875/150‑1 (MQ=255)
                                                                 ggCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGGATCGCCAGTCATGAGCATTCGTGCTGCGTCATTCAGGGCATGCATGGATGAACCACACAAGCGAGTCACGGTCACCGCCGGGACAGAGTGTGGTCCTTCTGcc                                                                               >  2:84582/1‑150 (MQ=255)
                                                                  gCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCca                                                                              <  2:629865/150‑1 (MQ=255)
                                                                          cccATATGCTCCACGCCGCCACCCAGACATACCAGCGCGTCGCGAGTCATGATCATTAGTGCTGCGTCATGCAGTGCCTGCATGGATGCCCCACCCACGCGATGAACGGTAACCGACGGGCCAGAGTGTGGTACTTCTGCGAGCCGcgcc                                                                      >  2:120772/1‑150 (MQ=255)
                                                                               atGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGtt                                                                 <  1:541931/150‑1 (MQ=255)
                                                                                tGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTa                                                                <  2:432722/150‑1 (MQ=255)
                                                                                tGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTa                                                                >  2:1365607/1‑150 (MQ=255)
                                                                                     cACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCTTTCGTGCTGCGTCATGCAGTGCCTGCATGGTTGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                           <  1:206668/150‑1 (MQ=255)
                                                                                     cACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTACCGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                           <  1:307490/150‑1 (MQ=255)
                                                                                     cACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                           >  2:585085/1‑150 (MQ=255)
                                                                                     cACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                           >  2:1653981/1‑150 (MQ=255)
                                                                                     cACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                           <  1:200698/150‑1 (MQ=255)
                                                                                      aCGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCg                                                          <  1:1549238/150‑1 (MQ=255)
                                                                                         ccgccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGGCCTGCATGGATGAACCCCACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGTTACTTCTGCCAGCAGCGCCGCGTTACGGGCGata                                                       >  1:102158/1‑150 (MQ=255)
                                                                                          cgccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGatat                                                      >  1:624024/1‑150 (MQ=255)
                                                                                           gccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATAtt                                                     <  2:729404/150‑1 (MQ=255)
                                                                                            ccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCGCACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                  >  2:857293/1‑120 (MQ=255)
                                                                                                  aGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAccc                                              >  1:3330/1‑150 (MQ=255)
                                                                                                  aGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAccc                                              >  2:753901/1‑150 (MQ=255)
                                                                                                   gACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCt                                             <  2:1577205/150‑1 (MQ=255)
                                                                                                     cATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGc                                           <  2:1160810/150‑1 (MQ=255)
                                                                                                       tGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTc                                         <  1:739330/150‑1 (MQ=255)
                                                                                                         ccTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCa                                       <  1:1031130/150‑1 (MQ=255)
                                                                                                         ccTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCa                                       <  1:404694/150‑1 (MQ=255)
                                                                                                         ccTGCGCATCACCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCTCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACAGGCGATATTAAAACCCTGCTCCa                                       >  2:350652/1‑150 (MQ=255)
                                                                                                          cTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAg                                      >  1:1261549/1‑150 (MQ=255)
                                                                                                                  cGCCAGTCATGATCATTCGTTCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGTCAGAGTGTGGGACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgct                              <  1:217434/150‑1 (MQ=255)
                                                                                                                  cGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgct                              <  1:1677672/150‑1 (MQ=255)
                                                                                                                   gCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgctg                             >  1:145936/1‑150 (MQ=255)
                                                                                                                   gCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgctg                             <  1:351440/150‑1 (MQ=255)
                                                                                                                    ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                              <  2:1483534/116‑1 (MQ=255)
                                                                                                                    ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                              >  1:1483534/1‑116 (MQ=255)
                                                                                                                    ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCtgctgc                            <  2:1507948/150‑1 (MQ=255)
                                                                                                                    ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCtgctgc                            <  1:1481736/150‑1 (MQ=255)
                                                                                                                      aGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGcac                          >  2:785898/1‑150 (MQ=255)
                                                                                                                           tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGTCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                     >  2:1179308/1‑150 (MQ=255)
                                                                                                                           tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                     >  1:204384/1‑150 (MQ=255)
                                                                                                                           tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                     >  1:1673001/1‑150 (MQ=255)
                                                                                                                            gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcc                    >  1:687177/1‑150 (MQ=255)
                                                                                                                              tCATTCGTGCTGCGGCATGCAGTGCCTGCATGGATGAACCACACAAGCTATTACCGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcccc                  <  2:102158/150‑1 (MQ=255)
                                                                                                                               cATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCa                 >  2:698103/1‑150 (MQ=255)
                                                                                                                                     tgctgcGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAat           <  2:1118687/150‑1 (MQ=255)
                                                                                                                                         catccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcg       >  1:157434/5‑150 (MQ=255)
                                                                                                                                          atccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgt      >  2:1529258/4‑150 (MQ=255)
                                                                                                                                           gTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACggg                                                            >  2:1587836/1‑95 (MQ=255)
                                                                                                                                           gTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACggg                                                            <  1:1587836/95‑1 (MQ=255)
                                                                                                                                           gTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGCTATTAAACCCCTGCTCCAGCGTCTGCTGCACTCACGCCCAGTAATTAtcgtc     >  2:1660177/1‑150 (MQ=255)
                                                                                                                                           gTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgtc     <  1:1400218/150‑1 (MQ=255)
                                                                                                                                              aTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGTCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAg  <  1:1179308/150‑1 (MQ=255)
                                                                                                                                              aTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAg  <  2:687177/150‑1 (MQ=255)
                                                                                                                                              aTGCAGTGCCTGCATGGATGAACCAAACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACCACCCCAGTAAATATCTTCGAg  >  1:1063903/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAG  >  NC_000913/4028334‑4028625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: