Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,553 T→A 7.7% Y17N (TAT→AAT) ‡ secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,5530TA 7.7% 381.2 / 14.2 136Y17N (TAT→AAT) ‡secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (60/65);  new base A (6/5);  total (66/70)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 138 total aligned reads displayed.

GGCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCT  >  NC_000913/429404‑429702
                                                                                                                                                     |                                                                                                                                                      
ggCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATTCTGTTGAACAACTAAACCACGGCCGTAAAGTCt                                                                                                                                                        >  2:953721/1‑150 (MQ=255)
  cGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTAt                                                                                                                                                      <  2:349621/150‑1 (MQ=255)
  cGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTAt                                                                                                                                                      >  1:805734/1‑150 (MQ=255)
   gACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCCCGGCCGTAAAGTCTATg                                                                                                                                                     >  2:632285/1‑150 (MQ=255)
   gACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATg                                                                                                                                                     >  2:732608/1‑150 (MQ=255)
   gACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATg                                                                                                                                                     >  2:1481037/1‑150 (MQ=255)
     cGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGAc                                                                                                                                                   >  2:420418/1‑150 (MQ=255)
     cGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCAGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATCTACTGTTGCACAACTAAACCACGGCCGTAAAGTCTATGAc                                                                                                                                                   >  1:350794/1‑150 (MQ=255)
       tCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACtt                                                                                                                                                 >  2:484830/1‑150 (MQ=255)
       tCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACtt                                                                                                                                                 <  2:1347696/150‑1 (MQ=255)
        cGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACttt                                                                                                                                                >  1:1086797/1‑150 (MQ=255)
         gATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTa                                                                                                                                               <  1:486320/150‑1 (MQ=255)
           tGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATg                                                                                                                                             <  2:386121/150‑1 (MQ=255)
            gTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacgg                                                                                                                                            <  1:1384153/150‑4 (MQ=255)
             tttACCGCGATTTTAGGTAAGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacggc                                                                                                                                           <  2:1304986/150‑5 (MQ=255)
             tttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATgcg                                                                                                                                           <  1:341236/150‑1 (MQ=255)
             tttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacggc                                                                                                                                           <  2:1696669/150‑5 (MQ=255)
              ttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATgcgc                                                                                                                                          <  2:796448/150‑1 (MQ=255)
              ttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATgcgc                                                                                                                                          <  1:1356659/150‑1 (MQ=255)
                aCCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCATAAAGTCATAGACTTTacggccgt                                                                                                                                        <  1:1690670/150‑8 (MQ=255)
                 ccGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCAGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACAACGGCCGTAAAGTCTATGACTTTATGCGCTgg                                                                                                                                       >  2:1104125/1‑150 (MQ=255)
                  cgcgATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTggg                                                                                                                                      >  2:1630705/1‑150 (MQ=255)
                           cGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTggg                                                                                                                             >  1:702155/1‑150 (MQ=255)
                            ggTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGc                                                                                                                            <  2:1277495/150‑1 (MQ=255)
                            ggTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGc                                                                                                                            <  2:1277497/150‑1 (MQ=255)
                                cgcgTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTc                                                                                                                        >  1:909116/1‑150 (MQ=255)
                                 gcgTGCCATCGTAAACCTGCTATATGGCAGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATTCTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCg                                                                                                                       >  1:235468/1‑150 (MQ=255)
                                  cgTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTTTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCgg                                                                                                                      <  1:1429540/150‑1 (MQ=255)
                                  cgTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCgg                                                                                                                      <  1:440809/150‑1 (MQ=255)
                                    tGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACCGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCa                                                                                                                    <  1:1449498/150‑1 (MQ=255)
                                       cATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCATTCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTAATGGGCTTTCGGCAtca                                                                                                                 >  1:1278133/1‑149 (MQ=255)
                                           gTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTgg                                                                                                             <  1:1646591/150‑1 (MQ=255)
                                              aaCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTct                                                                                                          >  2:1204101/1‑150 (MQ=255)
                                               aCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctg                                                                                                         >  1:42055/1‑150 (MQ=255)
                                               aCCTGCTATAAGGCGGCAAGCGCGTCAAGAAGCTGGCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctg                                                                                                         <  1:119120/150‑1 (MQ=255)
                                                  tGCTTTATGGGGGCAAGCGCGGCAAGAAGCTGTCAATCTGATGAGTGCGATGTGGCACAGGAATATACTGTTTAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctgctg                                                                                                      <  2:350794/150‑1 (MQ=255)
                                                  tGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctgctg                                                                                                      >  2:900129/1‑150 (MQ=255)
                                                   gCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGt                                                                                                     >  2:1009554/1‑150 (MQ=255)
                                                    cTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGtt                                                                                                    <  2:169369/150‑1 (MQ=255)
                                                    cTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGtt                                                                                                    <  1:676277/150‑1 (MQ=255)
                                                            gggcAAGCGCGGCATGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctg                                                                                            <  1:870831/149‑1 (MQ=255)
                                                            cggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctg                                                                                            <  1:1309317/150‑1 (MQ=255)
                                                            cggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctg                                                                                            >  1:649915/1‑150 (MQ=255)
                                                             ggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgc                                                                                           <  1:828528/150‑1 (MQ=255)
                                                             ggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgc                                                                                           <  2:205157/150‑1 (MQ=255)
                                                             ggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACCAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgc                                                                                           <  2:194021/150‑1 (MQ=255)
                                                              gcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgct                                                                                          >  2:1266057/1‑150 (MQ=255)
                                                              gcAAGCGAGTCAAGAAGATGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgct                                                                                          <  2:946955/150‑1 (MQ=255)
                                                                    gcgTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGtt                                                                                    <  1:1204101/150‑1 (MQ=255)
                                                                     cgTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGtga                                                                                   >  1:42653/1‑148 (MQ=255)
                                                                     cgTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGATGTTAATCGCTGCTATCGtta                                                                                   >  1:229192/1‑150 (MQ=255)
                                                                        cAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGttatta                                                                                >  1:1121685/1‑150 (MQ=255)
                                                                        cAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGttatta                                                                                <  1:964653/150‑1 (MQ=255)
                                                                         aagaagCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTtattat                                                                               >  2:1705085/1‑150 (MQ=255)
                                                                          agaagCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATg                                                                              >  2:363718/1‑150 (MQ=255)
                                                                           gaagCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATgg                                                                             >  2:1566931/1‑150 (MQ=255)
                                                                              gCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTTGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCg                                                                          >  2:1418250/1‑150 (MQ=255)
                                                                              gCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCg                                                                          >  1:1134969/1‑150 (MQ=255)
                                                                               cTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGt                                                                         >  2:1108919/1‑150 (MQ=255)
                                                                                tGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTg                                                                        >  2:520022/1‑150 (MQ=255)
                                                                                 gTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTgc                                                                       <  1:1481037/150‑1 (MQ=255)
                                                                                 gTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTgc                                                                       >  1:466988/1‑150 (MQ=255)
                                                                                   cAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTgcgc                                                                     >  1:551488/1‑150 (MQ=255)
                                                                                     aTCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCgg                                                                   <  1:994685/150‑1 (MQ=255)
                                                                                         gaggagTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCttt                                                               >  2:1173925/1‑150 (MQ=255)
                                                                                            gagTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCTGCTTTAAc                                                            <  2:649915/150‑1 (MQ=255)
                                                                                                gCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTggg                                                        <  2:1085050/150‑1 (MQ=255)
                                                                                                    tgtgGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCt                                                   <  1:632285/150‑1 (MQ=255)
                                                                                                     ggggCACAGGAATATACTGTTGAACAACTACACCACGGCCGTAAAGTCACTGACTTTATGCGCTGGGACTAGTGTGCTTTCGGCTTCTCTGGTCTGCTGTTTCTCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTg                                                  <  1:249140/148‑1 (MQ=255)
                                                                                                       ggCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGa                                                <  1:1115859/150‑1 (MQ=255)
                                                                                                           caGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTc                                            <  2:1134969/150‑1 (MQ=255)
                                                                                                           caGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTc                                            >  2:174997/1‑150 (MQ=255)
                                                                                                             gtaaTATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGTCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATgggg                                                                           <  1:822512/116‑2 (MQ=255)
                                                                                                             ggAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGc                                                                           >  2:822512/1‑118 (MQ=255)
                                                                                                             ggAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAc                                          >  1:510338/1‑150 (MQ=255)
                                                                                                              gAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAcc                                         <  1:544126/150‑1 (MQ=255)
                                                                                                                   taCTGTTGAACAACTAAACCCCGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTAGTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCggtgg                                    >  2:821292/1‑150 (MQ=255)
                                                                                                                   taCTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCggtgg                                    <  2:51601/150‑1 (MQ=255)
                                                                                                                       gTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACg                                <  1:953721/150‑1 (MQ=255)
                                                                                                                           aacaacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTa                            >  2:45364/1‑150 (MQ=255)
                                                                                                                              aacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTg                         >  1:960658/1‑150 (MQ=255)
                                                                                                                              aacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTg                         <  2:43590/150‑1 (MQ=255)
                                                                                                                                cTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGaa                       <  1:1009554/150‑1 (MQ=255)
                                                                                                                                  aaaCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGTTGTTAGGGGTATTGAAAt                     >  2:306453/1‑150 (MQ=255)
                                                                                                                                  aaaCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAAt                     <  2:235468/150‑1 (MQ=255)
                                                                                                                                   aaCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAAtt                    <  1:199598/150‑1 (MQ=255)
                                                                                                                                   aaCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCAGCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAAtt                    <  2:702155/150‑1 (MQ=255)
                                                                                                                                   aaCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGAGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTa                   >  1:328024/1‑150 (MQ=255)
                                                                                                                                     ccACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTAc                  <  1:1705085/150‑1 (MQ=255)
                                                                                                                                       aCGGGCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGGCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGc                <  1:1104125/150‑1 (MQ=255)
                                                                                                                                        cGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCt               >  1:207266/1‑150 (MQ=255)
                                                                                                                                          cgcatAAAGTCATAGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCTTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCg             >  2:12927/5‑150 (MQ=255)
                                                                                                                                          cgcatAAAGTCATAGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCg             >  1:660996/5‑150 (MQ=255)
                                                                                                                                           ccGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGATGGTTTACACCGGTGGTACGGTATTTAAAATTCCGcacaa            >  2:1216073/1‑146 (MQ=255)
                                                                                                                                            cGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTTTTGAAATTACGCTCGaa           >  1:1512752/1‑150 (MQ=255)
                                                                                                                                              tccaggcTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTTATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGTGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGaaaa         <  2:601753/144‑1 (MQ=255)
                                                                                                                                                  gTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGTTGTGGTTTTAAACGCTGATTGCGTAATAATGGCCGTGCGCGGATTAACAGGGGGCGTGGATTTCACCGTTGGTACGGTTATTAAATTTCCCATCTAAAAACCg     >  2:796591/1‑150 (MQ=255)
                                                                                                                                                    cTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGc   >  2:1182577/1‑150 (MQ=255)
                                                                                                                                                    cTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTAGCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGc   >  2:1403362/1‑150 (MQ=255)
                                                                                                                                                     tATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCt  <  2:743052/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GGCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCT  >  NC_000913/429404‑429702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: