Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,814 A→C 8.1% N190H (AAT→CAT) ‡ yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,8140AC 8.1% 398.9 / 21.5 135N190H (AAT→CAT) ‡yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (64/60);  new base C (4/7);  total (68/67)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

Only 100 of 136 total aligned reads displayed.

CAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGAC  >  NC_000913/4062665‑4062962
                                                                                                                                                     |                                                                                                                                                     
     cGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGAc                                                                                                                                                  >  2:1342688/1‑150 (MQ=255)
     cGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTAAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGAc                                                                                                                                                  >  1:526625/1‑150 (MQ=255)
      gTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACa                                                                                                                                                 >  2:1717430/1‑150 (MQ=255)
       ttgccttGTCGCTCCCGGGGTGGCATTTTCTGGCTTGGCAGTCTGTCCCTGGTGTTTCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATACCCTTATTGTGATTGCCGTAGGCATTATTAATATCTTTGTCATGAATGACaa                                                                                                                                                <  1:280571/145‑1 (MQ=255)
         gCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaag                                                                                                                                              <  1:1211295/150‑1 (MQ=255)
          cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTTCCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaaga                                                                                                                                             >  2:1001037/1‑150 (MQ=255)
          cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaaga                                                                                                                                             <  2:1447001/150‑1 (MQ=255)
          cATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaaga                                                                                                                                             >  1:1505722/1‑150 (MQ=255)
            ttGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAaagaag                                                                                                                                           <  2:647424/150‑1 (MQ=255)
            ttGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaaa                                                                                                                                           <  1:439119/150‑2 (MQ=255)
              ggcGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaaaat                                                                                                                                         <  2:876206/148‑4 (MQ=255)
              gTCGATACCGTGGTGGCATTTTCTGCGTTGACAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCATAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaaaat                                                                                                                                         <  1:592606/150‑4 (MQ=255)
                 gATACCGTGGGGGGATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCa                                                                                                                                      <  2:281165/150‑1 (MQ=255)
                  aTACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAc                                                                                                                                     >  2:1359719/1‑150 (MQ=255)
                   tACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAcc                                                                                                                                    >  2:866769/1‑150 (MQ=255)
                   tACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAcc                                                                                                                                    <  2:1386476/150‑1 (MQ=255)
                     ccGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgt                                                                                                                                  >  2:1712992/1‑150 (MQ=255)
                      cGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtc                                                                                                                                 >  1:19443/1‑150 (MQ=255)
                          gtggCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGtt                                                                                                                             >  2:1202140/1‑150 (MQ=255)
                            ggCTTTTTCTGAGTTGGCAGTATTTAGCTGGTTTGGGTGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCGCTTATTGTGATTGCCGTAGGCTTTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGttta                                                                                                                           <  2:428655/150‑3 (MQ=255)
                             gCATTTTCTGCGTTGGCAGTATTTACCAGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAg                                                                                                                          <  2:870555/150‑1 (MQ=255)
                              cATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAgg                                                                                                                         <  2:1509170/150‑1 (MQ=255)
                              cATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaa                                                                                                                                            <  1:1517276/131‑1 (MQ=255)
                              cATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCATTCAGGACAaagaa                                                                                                                                            >  2:1517276/1‑131 (MQ=255)
                                 tttCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGa                                                                                                                     <  2:215999/150‑1 (MQ=255)
                                    cTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCAccgtc                                                                                                                                 <  1:463900/136‑1 (MQ=255)
                                    cTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGaaaa                                                                                                                  >  1:1446461/1‑150 (MQ=255)
                                     tGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGaaaaa                                                                                                                 >  1:680980/1‑150 (MQ=255)
                                       cGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTCTGTTTTAAAGCAGGCATCTGGTTCTATTACCTTATTGTGATTGCCGTAGGAATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaag                                                                                                               <  2:661015/150‑1 (MQ=255)
                                        gTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaaga                                                                                                              <  2:1290228/150‑1 (MQ=255)
                                        gTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAaaga                                                                                                              <  1:1235678/150‑1 (MQ=255)
                                           ggCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAagaaga                                                                                                           >  2:549584/1‑150 (MQ=255)
                                            gCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGAc                                                                                                          <  1:1342688/150‑1 (MQ=255)
                                            gCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGAc                                                                                                          <  2:1419008/150‑1 (MQ=255)
                                            gCAGGATTTACCTGGGTTGGCAGTGGCTTATTAGGGTTTAAAGCAGGCAGCTGGTTCTATTCCCGATTGTGGTTTGCCTCCGCATTTTTTTTTTCTTTGTCGTGAATGCCCAAGAAGAGGCAACCGCCGGTGGGGGGAAATatgaattcg                                                                                                         >  2:212464/1‑146 (MQ=255)
                                                tATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAg                                                                                                      <  2:969935/150‑1 (MQ=255)
                                                 aTTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGc                                                                                                     >  2:781240/1‑150 (MQ=255)
                                                  tttACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTAGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCa                                                                                                    >  2:1069927/1‑150 (MQ=255)
                                                     aCCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCg                                                                                                 >  1:625291/1‑150 (MQ=255)
                                                       cTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGaa                                                                                               >  2:944970/1‑150 (MQ=255)
                                                        tGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGaaa                                                                                              >  1:958479/1‑150 (MQ=255)
                                                        tGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGAACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGaaa                                                                                              >  2:824321/1‑150 (MQ=255)
                                                        tGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGACGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAACAAGCCGGAGCTTCGaaa                                                                                              >  2:240358/1‑150 (MQ=255)
                                                         ggTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGaaaa                                                                                             >  2:786538/1‑150 (MQ=255)
                                                          gTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGaaaaa                                                                                            >  2:943536/1‑150 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           <  1:549584/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           <  1:723128/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           <  2:625291/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           >  2:1135974/1‑150 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           >  2:339416/1‑150 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGGGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           <  2:1505722/150‑1 (MQ=255)
                                                           tttGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACAGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAac                                                                                           >  1:846929/1‑150 (MQ=255)
                                                              ggCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACAcc                                                                                        >  1:792146/1‑150 (MQ=255)
                                                                cAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAg                                                                                      <  1:1295374/150‑1 (MQ=255)
                                                                  gTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTa                                                                                    <  2:758403/150‑1 (MQ=255)
                                                                     gCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGa                                                                                 <  2:1383192/150‑1 (MQ=255)
                                                                      cTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTGTGAc                                                                                <  2:711001/150‑1 (MQ=255)
                                                                       ttattaGGTTTTAAAGCTTGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCTCCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGAcc                                                                               <  2:1577617/150‑1 (MQ=255)
                                                                       ttattaGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGAcc                                                                               >  1:1570447/1‑150 (MQ=255)
                                                                         attaGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTc                                                                             <  2:846929/150‑1 (MQ=255)
                                                                           tacgTTTTAAAGCAGGCATCTGGTTCTATACCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                           >  2:795594/4‑150 (MQ=255)
                                                                           taGGTTTTTAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                           >  2:1607572/1‑150 (MQ=255)
                                                                           taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                           >  1:1388464/1‑150 (MQ=255)
                                                                           taGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCgg                                                                           <  1:1001037/150‑1 (MQ=255)
                                                                             gggtttAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTg                                                                         <  2:1225633/147‑1 (MQ=255)
                                                                             ggTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTg                                                                         >  1:368112/1‑150 (MQ=255)
                                                                              gTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAATGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGc                                                                        >  1:1097888/1‑150 (MQ=255)
                                                                                tttAAAGCAGGCATCTGGTTCTATTCTCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTg                                                                      >  2:1448180/1‑150 (MQ=255)
                                                                                 ttAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGa                                                                     <  2:19443/150‑1 (MQ=255)
                                                                                  tAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGa                                                                     <  2:673548/150‑1 (MQ=255)
                                                                                  tAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGaa                                                                    >  2:1267432/1‑150 (MQ=255)
                                                                                    aaGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAg                                                                  <  2:973584/150‑1 (MQ=255)
                                                                                         ggCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACaaa                                                             <  2:463959/150‑1 (MQ=255)
                                                                                           cATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAAc                                                           >  1:426498/1‑150 (MQ=255)
                                                                                            aTCTGGTTCTATTACCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACATCGGTGCTGAAAGACAAAACt                                                          >  2:1081234/1‑150 (MQ=255)
                                                                                               tGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTATCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGATAGACAAAACTAta                                                       >  2:705174/1‑149 (MQ=255)
                                                                                                 gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                     <  1:1369465/150‑1 (MQ=255)
                                                                                                 gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                     >  1:1588387/1‑150 (MQ=255)
                                                                                                 gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                     >  1:1667167/1‑150 (MQ=255)
                                                                                                 gTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTg                                                     <  1:1016819/150‑1 (MQ=255)
                                                                                                        tCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATc                                              <  1:1615805/150‑1 (MQ=255)
                                                                                                         cccTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCg                                             >  2:1686363/1‑150 (MQ=255)
                                                                                                             tATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCttt                                         <  1:1359719/150‑1 (MQ=255)
                                                                                                              aTTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCtttt                                        >  2:1631137/1‑150 (MQ=255)
                                                                                                                   gATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGTCAAAACTATCTGGCTTATCGCTTTTAACGt                                   <  2:1364806/150‑1 (MQ=255)
                                                                                                                    aTTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGATGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGtc                                  >  1:224182/1‑150 (MQ=255)
                                                                                                                          gTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCttcttc                            <  2:1627563/150‑1 (MQ=255)
                                                                                                                           tAGTCATTTTTTTTTTATTTGTCCTGAATGACATAGAAGTGGCACCGTCGTTTGAGGTGAAAATAGTAGACGGAGCATCGTAAAACACCAGTATGTCCTCGGTGCTGATAGACAAAACTATCTGGCTTATCTCTTTTAACGTCTTCTTCg                           <  2:908732/150‑1 (MQ=255)
                                                                                                                             ggCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGtt                         <  1:795594/150‑1 (MQ=255)
                                                                                                                               cATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTa                       >  1:893116/1‑150 (MQ=255)
                                                                                                                                attattattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAc                      >  1:1697546/1‑150 (MQ=255)
                                                                                                                                  tattattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTAcgc                    >  1:823778/1‑150 (MQ=255)
                                                                                                                                     tattTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGt                 <  1:1202140/150‑1 (MQ=255)
                                                                                                                                     cctcttCTTTGTCATTCAGGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGt                 >  1:1715750/5‑150 (MQ=255)
                                                                                                                                            tttGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACtga          >  1:9244/1‑149 (MQ=255)
                                                                                                                                              tGTCCTGAATGACAAAGAAGATGCACCTTCCTTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTgg        <  1:705067/150‑1 (MQ=255)
                                                                                                                                                tgcTGAATGACAAAGAAGAGGATCCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGcc      <  2:1655881/148‑1 (MQ=255)
                                                                                                                                                 ccTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCt     <  2:1446461/150‑1 (MQ=255)
                                                                                                                                                    gAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTCCGCGGTTTACTGTGGCCTGAc  >  1:79048/1‑150 (MQ=255)
                                                                                                                                                    gAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGAc  >  2:459332/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTTTGTCCTGAATGACAAAGAAGAGGCACCGTCCGTTGAGGTGAAAAAAGAAGACGGAGCATCGAAAAACACCAGTATGACCTCGGTGCTGAAAGACAAAACTATCTGGCTTATCGCTTTTAACGTCTTCTTCGTTTACGCGGTTTACTGTGGCCTGAC  >  NC_000913/4062665‑4062962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: