Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,066,845 C→A 5.5% G117V (GGT→GTT)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,066,8450CA 5.5% 420.8 / 7.8 138G117V (GGT→GTT) yihPputative 2,3‑dihydroxypropane‑1‑sulphonate exporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (63/66);  new base A (2/7);  total (65/73)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

GCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTAAA  >  NC_000913/4066696‑4066993
                                                                                                                                                     |                                                                                                                                                    
    aGCGCCGCCCTGACACCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGt                                                                                                                                                  <  2:1626041/150‑1 (MQ=255)
      cgccgcCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGa                                                                                                                                                <  1:640284/150‑1 (MQ=255)
        ccgccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGGTAAAACGGTGatg                                                                                                                                              <  2:819100/150‑3 (MQ=255)
         cgccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACAt                                                                                                                                             <  2:1607731/150‑1 (MQ=255)
         cgccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGGTAAAACGGTGatgg                                                                                                                                             <  1:450517/150‑4 (MQ=255)
          gccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATc                                                                                                                                            >  2:599739/1‑150 (MQ=255)
          gccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGGTAAAACGGTGatggc                                                                                                                                            <  2:1078355/150‑5 (MQ=255)
          gccCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGGTAAAACGGTGatggc                                                                                                                                            <  2:1196746/150‑5 (MQ=255)
           cccTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCa                                                                                                                                           <  1:333101/150‑1 (MQ=255)
              tGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAAc                                                                                                                                        >  2:127186/1‑150 (MQ=255)
                aCGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACgg                                                                                                                                      <  1:668518/150‑1 (MQ=255)
                  gCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgtg                                                                                                                                    <  2:499763/150‑1 (MQ=255)
                   ccATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGgtgt                                                                                                                                   >  1:905515/1‑150 (MQ=255)
                     aTGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGc                                                                                                                                 >  1:955580/1‑150 (MQ=255)
                       gccgccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCg                                                                                                                               <  1:1005500/150‑1 (MQ=255)
                       gccgccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCg                                                                                                                               >  2:1070345/1‑150 (MQ=255)
                        ccgccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGtgtg                                                                                                                                  <  2:386741/146‑1 (MQ=255)
                        ccgccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGtgtg                                                                                                                                  >  1:386741/1‑146 (MQ=255)
                        ccgccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGa                                                                                                                              >  2:1682953/1‑150 (MQ=255)
                          gccAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACa                                                                                                                            >  2:744254/1‑150 (MQ=255)
                            cAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACaaa                                                                                                                          >  1:53344/1‑150 (MQ=255)
                               tGAGGCGCGTTCGTTGGGGTTTTTGGTTATAGCGTGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAATCATAAAGTGAATAGTGGCCATCACCGTTTTACCGGTGTCATCAAACGGTGTGCCGACAAAGtt                                                                                                                       <  1:958392/150‑1 (MQ=255)
                                   gcgcGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCg                                                                                                                   <  1:1398980/150‑1 (MQ=255)
                                    cgcgTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGa                                                                                                                  >  1:1234099/1‑150 (MQ=255)
                                     gcgTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGAt                                                                                                                 >  1:169447/1‑150 (MQ=255)
                                     gagttcgttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGAt                                                                                                                 <  1:698600/148‑1 (MQ=255)
                                       gttcgttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCg                                                                                                               >  1:307801/1‑150 (MQ=255)
                                       gttcgttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACACTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACAGCCAACGGTGTGCAGCCAAAGTTGTCGATCg                                                                                                               >  2:1065966/1‑150 (MQ=255)
                                           gttGGGGTTTTTGCTGATAGCGGGAACCATTGCTCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCTTAAAGCATAAACAGAATAGTGGCCATCACCGATTTACCGGTGACATCAAACGGTGTGCCCACAAAGTTGGCGATCGCAAg                                                                                                           >  1:106087/1‑150 (MQ=255)
                                            ttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGt                                                                                                          >  1:767606/1‑150 (MQ=255)
                                            ttGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTAACGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGt                                                                                                          >  2:736583/1‑150 (MQ=255)
                                               gggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAg                                                                                                       >  1:914101/1‑150 (MQ=255)
                                                ggTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTAACGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAgg                                                                                                      >  1:329860/1‑150 (MQ=255)
                                                 gTTTTTGGTGATAGCGCGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGt                                                                                                     >  2:1351016/1‑150 (MQ=255)
                                                 ctttttGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGAGGCCATCACCCTTTTACCGGTGACATCAAACGGTGTGCCTACAAAGTTGGCGATCGCCAGTAAGGt                                                                                                     >  2:692650/2‑150 (MQ=255)
                                                   ttttGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGa                                                                                                   >  1:373077/1‑150 (MQ=255)
                                                        gTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCggg                                                                                              >  1:1673377/1‑150 (MQ=255)
                                                        gTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCggg                                                                                              <  1:1682953/150‑1 (MQ=255)
                                                         tGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGa                                                                                             <  2:1149578/150‑1 (MQ=255)
                                                           aTAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACAGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGCCCGGGatt                                                                                           >  2:298042/1‑148 (MQ=255)
                                                             aGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGa                                                                                         <  1:1504687/150‑1 (MQ=255)
                                                             aGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGa                                                                                         <  2:924568/150‑1 (MQ=255)
                                                               cGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACg                                                                                       <  1:205373/150‑1 (MQ=255)
                                                               cGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACg                                                                                       >  1:1534480/1‑150 (MQ=255)
                                                                 ggAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCa                                                                                     >  1:416048/1‑150 (MQ=255)
                                                                 ggAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATAACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCa                                                                                     >  2:1519258/1‑150 (MQ=255)
                                                                   aaCCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATa                                                                                   <  2:656846/150‑1 (MQ=255)
                                                                      cATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAg                                                                                <  2:1133965/150‑1 (MQ=255)
                                                                      cATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAg                                                                                <  2:955580/150‑1 (MQ=255)
                                                                      cATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAg                                                                                >  1:1296471/1‑150 (MQ=255)
                                                                       aTTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGa                                                                               >  1:1627643/1‑150 (MQ=255)
                                                                         tGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCTCCAGTAAGGTGACCGGGAATGACGCATACAGAAt                                                                             <  2:495324/150‑1 (MQ=255)
                                                                         tGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAAt                                                                             <  2:1382381/150‑1 (MQ=255)
                                                                         tGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAAAAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAAt                                                                             <  1:444808/150‑1 (MQ=255)
                                                                          gcgcCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATa                                                                            <  2:373077/150‑1 (MQ=255)
                                                                            gcCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACGAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaa                                                                          <  2:1569536/150‑1 (MQ=255)
                                                                             ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATATTGGCCATCACCGTTTTCCCGGTGACAAAAAACGGTGTGCCGCCAAAGTTGGCGATCGCCAGTAAGGTTACCGGGAATTACGCATACATAATaaaa                                                                         >  2:279236/1‑150 (MQ=255)
                                                                             ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaaa                                                                         >  2:643659/1‑150 (MQ=255)
                                                                             ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaaa                                                                         <  1:1110546/150‑1 (MQ=255)
                                                                             ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATaaaa                                                                         >  1:194809/1‑150 (MQ=255)
                                                                             ccGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGTGAATGACGCATACAGAATAAAAg                                                                        <  2:227211/150‑1 (MQ=255)
                                                                               gTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAgg                                                                       >  1:167345/1‑150 (MQ=255)
                                                                                 aGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGAc                                                                     <  2:414953/150‑1 (MQ=255)
                                                                                      cAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAAc                                                                <  1:110633/150‑1 (MQ=255)
                                                                                      cAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAAc                                                                >  1:1612082/1‑150 (MQ=255)
                                                                                       aGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACt                                                               <  1:606338/150‑1 (MQ=255)
                                                                                        gTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACtt                                                              >  1:838039/1‑150 (MQ=255)
                                                                                           catcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTAcc                                                           >  2:1552865/1‑150 (MQ=255)
                                                                                           catcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTAcc                                                           >  1:1364306/1‑150 (MQ=255)
                                                                                            atcatGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCt                                                          <  2:234584/150‑1 (MQ=255)
                                                                                               atGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGCGAATGATGCATACAGAATAAAAGGACGGAACTTACCtttt                                                       >  1:189476/1‑150 (MQ=255)
                                                                                                  cTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGa                                                    >  1:160604/1‑150 (MQ=255)
                                                                                                   tGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGAc                                                   <  1:599739/150‑1 (MQ=255)
                                                                                                         aCAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCt                                             <  1:643659/150‑1 (MQ=255)
                                                                                                                    aGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGaa                                  >  2:891023/1‑150 (MQ=255)
                                                                                                                     ccATAAACAGATTAGTGGCCATCACCGTTTTACCGCTGACATCAAACGGTGTGCCGACAAAGTTGGCGAGCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACTGAACTTACCTTTTGGACCGATCTTGCGTCGGGAAt                                 <  2:120060/149‑1 (MQ=255)
                                                                                                                      cATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGCGTGCCGACAAAGTTGGCGATCTCCAGTAAGGTGACCGGGAATGACGCATACAGAATATAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAAtg                                <  1:1248775/150‑2 (MQ=255)
                                                                                                                       aTAAACAGAATAGTGGCCATCACCGTTTTACCGGTGCCATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACCGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATcc                               >  2:884476/1‑150 (MQ=255)
                                                                                                                        tAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCa                              <  2:1234099/150‑1 (MQ=255)
                                                                                                                          aaCAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAAc                            <  1:1391396/150‑1 (MQ=255)
                                                                                                                          aaCAGAATAGTGGCCATCACCGTTATACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAAc                            <  1:723909/150‑1 (MQ=255)
                                                                                                                            cAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACAt                          >  1:422892/1‑150 (MQ=255)
                                                                                                                              gAATAGTGTCCATCACCGTTTTACCGTTGACATCAAACGGTGTGCCGACAAAGATGGCGATCGCCAGTAAGGTGACCGGTAATGACGCATACAGAATAAATGTACGTAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATaa                        <  1:62503/150‑1 (MQ=255)
                                                                                                                              gAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCATTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATaa                        <  2:169447/150‑1 (MQ=255)
                                                                                                                                       ccATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACTGAATAAAAGGACGGAACTTACCTTTTGGAACGATCTTGCGTCGGGAATCCAACATAATGCCGGTAc               >  2:760595/1‑150 (MQ=255)
                                                                                                                                        cATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTAcc              >  1:794869/1‑150 (MQ=255)
                                                                                                                                         aTCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTAccc             <  2:307801/150‑1 (MQ=255)
                                                                                                                                          tCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCa            <  1:1034021/150‑1 (MQ=255)
                                                                                                                                           cACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCat           <  2:416048/150‑1 (MQ=255)
                                                                                                                                              cGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATc        <  2:838039/150‑1 (MQ=255)
                                                                                                                                              cGTTTTACCGGTGACATCAAACGGCGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATc        <  2:1690791/150‑1 (MQ=255)
                                                                                                                                               gTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCg       >  2:714863/1‑150 (MQ=255)
                                                                                                                                               gTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCg       <  1:891023/150‑1 (MQ=255)
                                                                                                                                                   tACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGCCGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTaa   >  1:817704/1‑150 (MQ=255)
                                                                                                                                                   tACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACa                           >  1:864488/1‑126 (MQ=255)
                                                                                                                                                    aCCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTaaa  <  1:127186/150‑1 (MQ=255)
                                                                                                                                                    aCCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTaaa  >  1:1197372/1‑150 (MQ=255)
                                                                                                                                                    aCCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACATTTTGGACCGATCTTGAGTCGGGATTCCAACATAATGCCGGTACCCATATCGTTaaa  >  2:1623625/1‑150 (MQ=255)
                                                                                                                                                     ccGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAAt                                 >  2:689600/1‑118 (MQ=255)
                                                                                                                                                     ccGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAAt                                 <  1:689600/118‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGTAGCGCCGCCCTGACGCCATGCCGCCAGTGAGGCGCGTTCGTTGGGGTTTTTGGTGATAGCGGGAACCATTGCGCCGTAGGAGCAGTTCATCATGCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTAAA  >  NC_000913/4066696‑4066993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: