Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,074,842 C→T 8.9% H58H (CAC→CAT) ‡ yihW → putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,074,8420CT 8.9% 303.4 / 12.2 104H58H (CAC→CAT) ‡yihWputative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base C (42/51);  new base T (3/6);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

AACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGT  >  NC_000913/4074693‑4074991
                                                                                                                                                     |                                                                                                                                                     
aaCCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCAc                                                                                                                                                       <  1:984875/150‑1 (MQ=255)
  cccGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCAGCACgg                                                                                                                                                     >  2:611721/1‑150 (MQ=255)
    cgcgGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTg                                                                                                                                                   <  1:973259/150‑1 (MQ=255)
    cgcgGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTg                                                                                                                                                   >  2:1443121/1‑150 (MQ=255)
     gcgGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTgg                                                                                                                                                  <  1:1139525/150‑1 (MQ=255)
      cgGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGgc                                                                                                                                                 <  1:1282348/150‑1 (MQ=255)
         cACGACCAACTCCTCATGCTGATCGCCGATCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGcgcg                                                                                                                                              <  2:763430/150‑1 (MQ=255)
          aCGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAACTTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgt                                                                                                                                             <  1:1088793/150‑2 (MQ=255)
          aCGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgt                                                                                                                                             <  1:787732/150‑2 (MQ=255)
           cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCggg                                                                                                                                            <  1:1127946/150‑1 (MQ=255)
           cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgta                                                                                                                                            <  2:1659138/150‑3 (MQ=255)
            gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGt                                                                                                                                           >  1:1342710/1‑150 (MQ=255)
            gACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgtaa                                                                                                                                           <  1:1377094/150‑4 (MQ=255)
                aaCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCggg                                                                                                                                       <  2:1452306/150‑1 (MQ=255)
                aaCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCggg                                                                                                                                       <  1:607969/150‑1 (MQ=255)
                aaCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgtaatcag                                                                                                                                       <  1:1293588/150‑8 (MQ=255)
                 aCTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGc                                                                                                                                      <  1:1160180/150‑1 (MQ=255)
                    cctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTc                                                                                                                                   <  2:358766/150‑1 (MQ=255)
                     ctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGCTGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTcc                                                                                                                                  <  1:14590/150‑1 (MQ=255)
                       cATGCTTATCGCCGAGCGGGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGACTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAg                                                                                                                                <  1:117392/150‑1 (MQ=255)
                       cATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAg                                                                                                                                <  1:495159/150‑1 (MQ=255)
                         tGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcg                                                                                                                              <  1:293691/150‑1 (MQ=255)
                         tGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgtaatcag                                                                                                                                       <  2:171665/141‑8 (MQ=255)
                         tGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCACCGTGATGGcgcgtaatcag                                                                                                                                       >  1:171665/1‑134 (MQ=255)
                           cTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcgtc                                                                                                                            >  1:1630471/1‑150 (MQ=255)
                            tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcgtcg                                                                                                                           >  2:252671/1‑150 (MQ=255)
                            tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcgtcg                                                                                                                           <  1:1161043/150‑1 (MQ=255)
                            tGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGcgtcg                                                                                                                           >  2:359600/1‑150 (MQ=255)
                                 gCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCCGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCTAGGCCTGAGTACGCGCCCTCACGGTGGCGCGGGTCGGGCTTCCAGCGTAGTTAAt                                                                                                                      >  2:1230968/1‑150 (MQ=255)
                                 gCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAAt                                                                                                                      >  2:1013964/1‑150 (MQ=255)
                                   cGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATAc                                                                                                                    >  2:1531933/1‑150 (MQ=255)
                                    gAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACg                                                                                                                   <  2:762830/150‑1 (MQ=255)
                                     aGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCCCGCAGACGGACCGCCGGGATATTCGTAAATTAAGCGATCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGGCGGGCTTCCAGCGTCGTTATTACgg                                                                                                                  >  2:1467294/1‑150 (MQ=255)
                                         tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                              >  2:208126/1‑150 (MQ=255)
                                         tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGAAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                              >  1:1303855/1‑150 (MQ=255)
                                          gggTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTc                                                                                                             <  1:1013964/150‑1 (MQ=255)
                                            gTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGCCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGa                                                                                                           >  2:1396748/1‑150 (MQ=255)
                                             tataTGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGag                                                                                                          >  2:745902/1‑150 (MQ=255)
                                             taaatGAATATTGACGAGCTGGCAAAACTGCTGGATGGCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCCCGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGag                                                                                                          <  1:1578442/147‑1 (MQ=255)
                                               tatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagca                                                                                                        >  2:637897/1‑150 (MQ=255)
                                                 tGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagcagc                                                                                                      >  1:457126/1‑150 (MQ=255)
                                                  gAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCg                                                                                                     <  1:745902/150‑1 (MQ=255)
                                                    atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGGTAATACGGCGTTCGAGCAGCGTg                                                                                                   >  2:153512/1‑150 (MQ=255)
                                                     tatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCgaga                                                                                                  >  2:770864/1‑150 (MQ=255)
                                                     tatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGa                                                                                                  >  2:605262/1‑150 (MQ=255)
                                                     tatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGa                                                                                                  >  2:1187110/1‑150 (MQ=255)
                                                      tttGATTAGCTGGCAAATATGCTGGAGGTCTCCAGGCAGACGGTCCTCCGGGATACTCGTAAATTAAGCGAGCAAGGCCTGTTTACGCTCCAGCAGTGTTGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAg                                                                                                 <  1:544455/149‑1 (MQ=255)
                                                        tgatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCAGCACGGGGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGt                                                                                               <  1:1581662/150‑1 (MQ=255)
                                                        tgatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGCGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCCCGGTGGCGCGGGTCGGGCTTCCAGAGTCGTTAAAACGGCGTTCGAGCCGCGTGAGGt                                                                                               >  1:298705/1‑150 (MQ=255)
                                                         gatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGtt                                                                                              >  1:609743/1‑150 (MQ=255)
                                                           tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGTAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                            >  1:836378/1‑150 (MQ=255)
                                                            gaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCg                                                                                           >  2:297936/1‑150 (MQ=255)
                                                            gaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCg                                                                                           <  2:69552/150‑1 (MQ=255)
                                                             cgCTGGCAACTGTGCTGGCTCTCTCCCCGCAGACGGTCCGCCGGGATATTCGTAACTTACGCGAGCAATGCCTGATTCCGCGCCATCACGGCGGCGCGTGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGc                                                                                          <  2:289583/149‑1 (MQ=255)
                                                             aGCTGGCAAATCTGCTGGATGGCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTACGCGAGCAAGGCCTGATTACGCGCCATCACGGGGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGc                                                                                          <  1:1292811/150‑1 (MQ=255)
                                                              gCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCa                                                                                         <  2:1472050/150‑1 (MQ=255)
                                                                 ggCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCAATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAAc                                                                                      >  2:307333/1‑150 (MQ=255)
                                                                       tCTGCGGGATGGCGCCACGCAGACGCTCCTCCGGGATATTCGTAAAATCAGCGAGCCAGGCCTGATTACGCGCCATCTCGGTGGCGCGGGTCGGGCTTCCAGCGTCGTAAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGa                                                                                <  1:431882/150‑1 (MQ=255)
                                                                         tgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaa                                                                              <  1:917368/150‑1 (MQ=255)
                                                                               aTGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAGAAAAAAg                                                                        <  1:1696323/150‑1 (MQ=255)
                                                                                     ccACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTg                                                                  <  2:1342710/150‑1 (MQ=255)
                                                                                       aCGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGcc                                                                <  1:1458310/150‑1 (MQ=255)
                                                                                         gCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGa                                                              <  2:530097/150‑1 (MQ=255)
                                                                                         gCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGa                                                              <  1:208126/150‑1 (MQ=255)
                                                                                         gCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGACCAAGGCCTGAGTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGGCGTTAATACGGCGTGCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGa                                                              <  2:134248/150‑1 (MQ=255)
                                                                                            gACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGc                                                           <  1:1443121/150‑1 (MQ=255)
                                                                                               ggTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGt                                                        <  1:297936/150‑1 (MQ=255)
                                                                                                 tCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTgg                                                      <  1:1531933/150‑1 (MQ=255)
                                                                                                   cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                    <  2:1630471/150‑1 (MQ=255)
                                                                                                      ctggATTTTCTTAAATTAAGCGATCAAGGCCTGTTTACGCGCCATCACTGTGGCGCGGGTCGGGCTTCCAGCGTGGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACGGAGGAATAAAAATCGTTTGCCGAAGCGGTGGCAGAc                                                 <  2:694515/148‑1 (MQ=255)
                                                                                                         gATATTCGTAAATAAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                              <  2:867962/150‑1 (MQ=255)
                                                                                                            atTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGGCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATAtt                                           <  2:363007/150‑1 (MQ=255)
                                                                                                                gTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTg                                       <  2:425374/150‑1 (MQ=255)
                                                                                                                 tAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGa                                      >  1:1582219/1‑150 (MQ=255)
                                                                                                                 tAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGa                                      >  1:274686/1‑150 (MQ=255)
                                                                                                                  aaaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGAt                                     >  1:607138/1‑150 (MQ=255)
                                                                                                                   aaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATg                                    >  2:1205585/1‑150 (MQ=255)
                                                                                                                        tgCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTca                               <  1:1638065/149‑1 (MQ=255)
                                                                                                                          cGAGCAAGGCCTGATTACGCGCCATCAGGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                             <  2:1640708/150‑1 (MQ=255)
                                                                                                                          cGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                             <  1:829732/150‑1 (MQ=255)
                                                                                                                          cGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                             >  1:525212/1‑150 (MQ=255)
                                                                                                                           gAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                            >  1:701742/1‑150 (MQ=255)
                                                                                                                            aGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaacaa                           >  2:195958/1‑150 (MQ=255)
                                                                                                                              cAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                         <  1:252671/150‑1 (MQ=255)
                                                                                                                              cAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAata                         <  1:359600/150‑1 (MQ=255)
                                                                                                                                 ggCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCACACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAttt                      >  2:162225/1‑150 (MQ=255)
                                                                                                                                 ggCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAttt                      >  2:941250/1‑150 (MQ=255)
                                                                                                                                  cccTGATTACGCGCCATCACGGGGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTa                     <  1:1533384/149‑1 (MQ=255)
                                                                                                                                   ccTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTAt                    >  1:1303390/1‑150 (MQ=255)
                                                                                                                                    cTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATc                   >  2:760177/1‑150 (MQ=255)
                                                                                                                                     tGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                  >  2:483368/1‑150 (MQ=255)
                                                                                                                                     ccgacccgcgCCACCGTGATGGCGCGGGTCGGGCTTCCAGCGACGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                  >  1:1355332/7‑150 (MQ=255)
                                                                                                                                          aCGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCAtt             <  1:143186/150‑1 (MQ=255)
                                                                                                                                           cgcgCCACCGTGATGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTg            >  1:1672749/1‑150 (MQ=255)
                                                                                                                                            gcgcCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTgg           <  2:701742/150‑1 (MQ=255)
                                                                                                                                            gcgcCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTgg           <  2:1699937/150‑1 (MQ=255)
                                                                                                                                                 aTCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCCATCCGAGGGAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGa      >  1:279495/1‑150 (MQ=255)
                                                                                                                                                  tCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGAc     <  1:637897/150‑1 (MQ=255)
                                                                                                                                                     cGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGt  <  1:1689231/150‑1 (MQ=255)
                                                                                                                                                     cGGTGGCGCGGGTCGCGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGt  >  2:663438/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCCGCGGCACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGT  >  NC_000913/4074693‑4074991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: