Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,075,521 G→A 5.1% intergenic (+67/‑32) yihW → / → yihX putative transcriptional regulator for sulphoquinovose utilization/alpha‑D‑glucose‑1‑phosphate phosphatase, anomer‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,075,5210GA 5.1% 435.3 / 5.1 134intergenic (+67/‑32)yihW/yihXputative transcriptional regulator for sulphoquinovose utilization/alpha‑D‑glucose‑1‑phosphate phosphatase, anomer‑specific
Reads supporting (aligned to +/- strand):  ref base G (58/69);  new base A (5/2);  total (63/71)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTAAGAAGAGTTTTCAT  >  NC_000913/4075372‑4075669
                                                                                                                                                     |                                                                                                                                                    
ccGACGAGCTGCCGACCGCTGCGATAAAATCACGCTTACAAGACATCCAAATTGAAATCATTCTTCCCCAGGAAGAAGATTAGATTTTTGTGACCGTTAACCTTTCTTCATACCTTGCCACATAGCCAACCACATACTTTACCGCTACAg                                                                                                                                                      >  1:1513497/1‑150 (MQ=255)
  gacgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGCCGCGTAGATTTTTGTGACCGTTAACCTGGCTACATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGtt                                                                                                                                                    >  1:1487309/1‑150 (MQ=255)
  gacgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGtt                                                                                                                                                    >  2:710328/1‑150 (MQ=255)
    cgaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTaa                                                                                                                                                  >  1:433728/1‑150 (MQ=255)
     gaGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAAt                                                                                                                                                 <  1:442844/150‑1 (MQ=255)
       gCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAAttt                                                                                                                                               >  1:119761/1‑150 (MQ=255)
        cTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTc                                                                                                                                              >  2:1489022/1‑150 (MQ=255)
          gccgccCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCtt                                                                                                                                            <  2:431116/150‑1 (MQ=255)
            cgcccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                                                                                                                          <  2:1001458/150‑1 (MQ=255)
               ccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGgc                                                                                                                                       >  2:1582494/1‑150 (MQ=255)
               ccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGgc                                                                                                                                       >  2:1456691/1‑150 (MQ=255)
                cgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGgcg                                                                                                                                      >  2:317732/1‑150 (MQ=255)
                     cgcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGaaa                                                                                                                                 >  1:1377550/1‑150 (MQ=255)
                      gcTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAag                                                                                                                                >  1:128102/1‑150 (MQ=255)
                          aaaTCACGCTTACAAGACAGCCAAATTGATATCATTCTTCCCCAGGAAGACGCGGAGATTTTTGTGAACGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                                                                                                            <  2:904913/150‑1 (MQ=255)
                          aaaTCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                                                                                                            >  2:1367657/1‑150 (MQ=255)
                          aaaTCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGAATTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAaggagg                                                                                                                            <  1:317881/150‑1 (MQ=255)
                           aaTCACGCTTACAAGACGGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCGCGCCACAAGAAATTAACTGTAGCGGGAAaggatgg                                                                                                                           <  1:986929/150‑4 (MQ=37)
                           aaTCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCGCGCCACAAGAAATTAACTGTAGCGGGAAaggatgg                                                                                                                           <  1:1614404/150‑4 (MQ=37)
                           aaTCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGc                                                                                                                           <  2:1487309/150‑1 (MQ=255)
                             tCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaa                                                                                                                         >  1:1672967/1‑150 (MQ=255)
                                cGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                                                                                                                      >  2:1050600/1‑150 (MQ=255)
                                  cTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATg                                                                                                                    >  2:58759/1‑150 (MQ=255)
                                      cAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGctct                                                                                                                <  2:1377550/150‑1 (MQ=255)
                                        aGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                                                                                                              >  1:19904/1‑150 (MQ=255)
                                        aGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat                                                                                                              <  2:20757/150‑1 (MQ=255)
                                        aGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCACGCTACAGTTAATTTCTTGTGGAGCGAAAGGAGGCAAAAATGCTCtat                                                                                                              >  2:1428149/1‑150 (MQ=255)
                                           cAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATc                                                                                                           >  1:502599/1‑150 (MQ=255)
                                             gCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCCTACCTTGCCCCATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCtt                                                                                                         >  1:1258606/1‑150 (MQ=255)
                                             gCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCtt                                                                                                         >  1:425375/1‑150 (MQ=255)
                                               cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTg                                                                                                       <  2:839919/150‑1 (MQ=255)
                                               aaaaTTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCAAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTg                                                                                                       <  2:563956/149‑1 (MQ=255)
                                                aaaTTGAAATCATTCTTCCCCAGGCAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCCTCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGa                                                                                                      <  1:138502/150‑1 (MQ=255)
                                                 aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGTAAAAAATGCTCTATATCTTTGAt                                                                                                     >  2:609527/1‑150 (MQ=255)
                                                 aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAt                                                                                                     <  2:1372325/150‑1 (MQ=255)
                                                   atGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAttt                                                                                                   <  1:40410/149‑1 (MQ=255)
                                                      aaaTCATTCTTCCCCAGGAAGACTCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAgg                                                                                                <  1:757332/150‑1 (MQ=255)
                                                      aaaTCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAgg                                                                                                <  1:1482369/150‑1 (MQ=255)
                                                       aaTCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGt                                                                                               <  2:502599/150‑1 (MQ=255)
                                                         tCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTaa                                                                                             >  2:240387/1‑150 (MQ=255)
                                                         tCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTaa                                                                                             <  1:719164/150‑1 (MQ=255)
                                                             tcttcCCCAGGAAGACGCGTAGATTTTAGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAAtgtg                                                                                         <  2:432322/150‑1 (MQ=255)
                                                                 ccccAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAACCCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTg                                                                                     >  2:1604264/1‑150 (MQ=255)
                                                                   ccAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTc                                                                                   >  1:1432842/1‑150 (MQ=255)
                                                                    cAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCg                                                                                  <  2:69234/150‑1 (MQ=255)
                                                                      ctaaGACGAGTAGATTTTTCTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGat                                                                                <  2:322543/148‑1 (MQ=255)
                                                                         aGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATc                                                                             <  1:525282/150‑1 (MQ=255)
                                                                         aGACGCGTAGATTTTTGTGACCGTAAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATACTTTCCCGCTACAATTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGataac                                                                             >  2:276421/1‑148 (MQ=255)
                                                                           aCGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGa                                                                           <  2:1231557/150‑1 (MQ=255)
                                                                            cgcgTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGAc                                                                          <  1:95155/150‑1 (MQ=255)
                                                                             gcgTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACt                                                                         <  1:1427937/150‑1 (MQ=255)
                                                                             gcgTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCGACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACt                                                                         >  2:1140451/1‑150 (MQ=255)
                                                                               gTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACttt                                                                       <  2:792773/150‑1 (MQ=255)
                                                                                  gATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAAc                                                                    >  2:409432/1‑150 (MQ=255)
                                                                                    tttttGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAATCCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCg                                                                  <  1:1604264/150‑1 (MQ=255)
                                                                                    tttttGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCg                                                                  >  1:1346755/1‑150 (MQ=255)
                                                                                      tttGTGACCGTTAACCTGGCTTCATACCTTGCCACCTAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCgtg                                                                <  2:1258606/150‑1 (MQ=255)
                                                                                      tttGTGACCGTTAACCTGGCGTCATACCTTGCCACATAGCCAAACCCATCCTTTCCAGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTCATTGTCGATATCGACTTTAACCgtg                                                                >  1:291569/1‑150 (MQ=255)
                                                                                        tgtgACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGtgtg                                                              <  1:1700050/150‑1 (MQ=255)
                                                                                          tgACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCt                                                            >  2:499004/1‑150 (MQ=255)
                                                                                           gACCGTTAACCTGCATTCATACCTTGCCACATAGCCAAACCCATCCTTTCACGCTACAGTTAATTTCTTGTGGCGCGAAATGAAGCAAAATGTCTCTATATGTTTGATGTAGGGAATGTGATTGTCGATATCGACTGTACCCGTGTCCTg                                                           >  2:253321/1‑150 (MQ=255)
                                                                                             ccGTTAACCTGGCTTCATACCTTGCCACAAAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTggg                                                         <  2:612464/150‑1 (MQ=255)
                                                                                              cGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGa                                                        <  2:119761/150‑1 (MQ=255)
                                                                                                    ccTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTgg                                                  >  2:580114/1‑150 (MQ=255)
                                                                                                    ccTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTgg                                                  >  1:768854/1‑150 (MQ=255)
                                                                                                    ccTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTgg                                                  >  1:124044/1‑150 (MQ=255)
                                                                                                    ccTGGATTAATACCTTGCCACATATCCAAACCCATCCTTTTCCGCTACAGTTAATTTCTTGTGGCTAGAAAGGAGGCAAAAATGCTCTATATATTTGTTTTATGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTgg                                                  <  2:1513497/150‑1 (MQ=255)
                                                                                                      tGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAg                                                <  1:262246/150‑1 (MQ=255)
                                                                                                       ggCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGc                                               <  2:1292611/150‑1 (MQ=255)
                                                                                                       ggCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGc                                               <  1:1030946/150‑1 (MQ=255)
                                                                                                           tCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGAtt                                           <  1:710328/150‑1 (MQ=255)
                                                                                                            cATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGAttt                                          <  1:552735/150‑1 (MQ=255)
                                                                                                              tACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTaa                                        <  2:1222672/150‑1 (MQ=255)
                                                                                                               aCCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAc                                       >  2:473203/1‑150 (MQ=255)
                                                                                                               aCCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAc                                       >  1:428427/1‑150 (MQ=255)
                                                                                                                ccTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATTCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcg                                      <  1:1050600/150‑1 (MQ=255)
                                                                                                                 cTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcgc                                     <  1:1565466/150‑1 (MQ=255)
                                                                                                                  ttGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcgcg                                    >  1:809241/1‑150 (MQ=255)
                                                                                                                  ttGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcgcg                                    <  2:1672967/150‑1 (MQ=255)
                                                                                                                  ttGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcgcg                                    <  2:1432842/150‑1 (MQ=255)
                                                                                                                  ttGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAAcgcg                                    <  1:942169/150‑1 (MQ=255)
                                                                                                                       acaTAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTc                               >  1:1204785/1‑150 (MQ=255)
                                                                                                                           aGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCt                           >  2:1152026/1‑150 (MQ=255)
                                                                                                                            gCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTTTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGAGATTTAACGAGTATTcagatt                          >  2:832286/1‑145 (MQ=255)
                                                                                                                            gCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTg                          >  2:625944/1‑150 (MQ=255)
                                                                                                                            gCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTg                          >  2:679277/1‑150 (MQ=255)
                                                                                                                              cAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGc                        <  2:428427/150‑1 (MQ=255)
                                                                                                                               aaaCCCATCCTTTACCTCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCa                       <  1:1130861/150‑1 (MQ=255)
                                                                                                                                  cccATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTTGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCg                    >  1:92050/1‑150 (MQ=255)
                                                                                                                                  cccATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCg                    >  2:423099/1‑150 (MQ=255)
                                                                                                                                  cccATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCg                    >  2:1393800/1‑150 (MQ=255)
                                                                                                                                   ccATCCTTTCCCGCTACAGTTAATTTGTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCgt                   <  1:1428149/150‑2 (MQ=255)
                                                                                                                                    cctCCTTTCGCGCCACAAGAAATTAACTGTAGCGGGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCt                  >  1:631053/3‑150 (MQ=37)
                                                                                                                                    cctCCTTTCGCGCCACAAGAAATTAACTGTAGCGGGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGATATGTGATTGTCGATATCGACTTTAACCGTGAGCTGGGAGCATGGAGCGATTTTAAGCGTATTCCTCTCGCATCGCt                  >  2:687739/3‑150 (MQ=21)
                                                                                                                                      tCCTTTCGCGCCACAAGAAATTAACTGTAGCGGGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTa                >  1:1368050/1‑150 (MQ=38)
                                                                                                                                      tCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTa                <  1:1456691/150‑1 (MQ=255)
                                                                                                                                      tCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGAGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTa                <  1:1582494/150‑1 (MQ=255)
                                                                                                                                        cTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTaag              >  1:604498/1‑150 (MQ=255)
                                                                                                                                                 tACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTAAGAAGAGtttt     >  1:294327/1‑150 (MQ=255)
                                                                                                                                                   cAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTAAGAAGAGTTTTCa   <  1:499004/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTTAGGTAATGTGATTGTCGATATCGACTTTAACCGTGTGCTGGGAGCCTGGAGCGATTTAACGCGTATTCCGCTGGCATCGCTTAAGAAGAGTTTTCAT  >  NC_000913/4075372‑4075669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: