Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 430,506 G→A 12.5% intergenic (+30/‑99) secF → / → yajD SecYEG protein translocase auxillary subunit/HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913430,5060GA12.5% 237.6 / 16.2 89intergenic (+30/‑99)secF/yajDSecYEG protein translocase auxillary subunit/HNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (42/35);  new base A (6/5);  total (49/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCG  >  NC_000913/430357‑430651
                                                                                                                                                     |                                                                                                                                                  
aCTTCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAACCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGTGCTGATCAGCAGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCgg                                                                                                                                                    <  1:464448/150‑1 (MQ=255)
  tGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTc                                                                                                                                                  <  2:979339/150‑1 (MQ=255)
  tGCATCTTCCATCTATGTGGCATCTGCGGTGGCTCTGAAACTGGGTATGAAGCGCGCACACATGTTGCAGCATAAAGTGAAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGCTc                                                                                                                                                  >  2:1333216/1‑150 (MQ=255)
      gcTTCCATCTATGTGGCATCTGCGTTGGCGCTGAAACTGGGTATGAAGCGCGATCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaa                                                                                                                                              <  1:1455515/149‑1 (MQ=255)
           cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGCAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCCGCCGTCAATTCTGCCGTAATCAAGTTACCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                         >  1:95552/1‑150 (MQ=255)
           cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                         >  2:1280649/1‑150 (MQ=255)
            aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                        >  2:1702642/1‑150 (MQ=255)
            aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                        <  1:1548820/150‑1 (MQ=255)
             tCTATGTGGCATCTGCGCTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCggg                                                                                                                                       <  2:164162/150‑1 (MQ=255)
               tATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAt                                                                                                                                    >  2:984674/1‑150 (MQ=255)
                  gtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttt                                                                                                                                 >  1:503699/1‑150 (MQ=255)
                    ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                                                                                               >  1:593712/1‑150 (MQ=255)
                    ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                               <  2:1648435/150‑3 (MQ=255)
                    ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                               <  2:1710169/150‑3 (MQ=255)
                     gCATGTGCGTTGGCTCTGAACCTGGGAATGAAGCGCGAACACATTTTGCAGCAGAAAGTGGAAAAAGAAGTGGCGGATCAGCCGTCATTTCTGCCGTATTCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                              <  2:1673434/150‑4 (MQ=255)
                     gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                              <  2:1188811/150‑1 (MQ=255)
                     gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                              <  2:657523/150‑4 (MQ=255)
                     gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGCTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                              <  1:1596409/150‑1 (MQ=255)
                      cATCTTCGTTTGCTCTGATACTGGGTATGAAGCGCTAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTTATGTTGAAAATCCCGGTCAGAAGATCGTGAtttttttt                                                                                                                             <  1:590666/150‑1 (MQ=255)
                      cATCTGCGTTGGCTCTGAAACTGGGTATGATGCGCTAACACATGTTGCAGCAGAACGCGGAAAAAGAAGTGTCGTATCAGCCGTCAACTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttttt                                                                                                                             <  1:266173/150‑1 (MQ=255)
                      cATCTGCGTTGGCTCTGAAACTGGGTATGAACCGCGAAGAAATGTTGCAGGAGAAACTGGAAAAAGAACTGGCGGATCAGCCGGCAATTCTTCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaac                                                                                                                             <  2:776071/150‑5 (MQ=255)
                       aTCTGCGTTGGCTCTGAAACTGTGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTg                                                                                                                            <  1:1553515/150‑1 (MQ=255)
                                    ctGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATCTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGAtt                                                                                                               >  2:450089/1‑150 (MQ=255)
                                      taaaCTGGGTATTAAGCGCGAACACATGTTTCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCAGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                             <  2:321371/149‑1 (MQ=255)
                                      gAAACTGGGTATGAAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGGCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                             <  1:1203356/150‑1 (MQ=255)
                                       aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATATTTTTTGATTTATGGAGATTGCg                                                                                                            >  2:687231/1‑150 (MQ=255)
                                       aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGGTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCg                                                                                                            <  2:1636866/150‑1 (MQ=255)
                                          cTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGaaa                                                                                                         >  1:1083918/1‑150 (MQ=255)
                                                aTGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATcccc                                                                                                   <  2:577605/150‑1 (MQ=255)
                                                 tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGTGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTATTATTGATGTATGGAGATTGCGAAAATCCCCg                                                                                                  >  1:1167675/1‑150 (MQ=255)
                                                 tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTGATGTATGGAGATTGCGAAAATCCCCGc                                                                                                 <  1:1596794/150‑1 (MQ=255)
                                                  gAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCACGc                                                                                                 >  1:95204/1‑150 (MQ=255)
                                                   aaGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCACCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCa                                                                                                <  2:1690147/150‑1 (MQ=255)
                                                     gcgcGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATc                                                                                              >  1:835007/1‑150 (MQ=255)
                                                         gcacacCTGTGGCCGCAGAAAGGGCCAAAAGAAGGGGCGGAGCAGCCGTCAAGGCTGCCGTAATCAAGTGCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                          <  1:1333216/148‑1 (MQ=255)
                                                          aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                          <  2:439891/150‑1 (MQ=255)
                                                          aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTggg                                                                                         >  1:1139812/1‑150 (MQ=255)
                                                           acacATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGa                                                                                        <  1:1175635/150‑1 (MQ=255)
                                                              caTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAAc                                                                                     <  1:519590/150‑1 (MQ=255)
                                                               aTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACt                                                                                    >  2:852320/1‑150 (MQ=255)
                                                                 gTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgc                                                                                  <  1:669501/150‑1 (MQ=255)
                                                                  ttGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgcg                                                                                 <  2:593712/150‑1 (MQ=255)
                                                                    gcagcaGAAAGTGGAAAAAGAAGGGGCGGATCAGCCATCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGcgcg                                                                               >  2:188755/1‑150 (MQ=255)
                                                                      agcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTa                                                                             >  1:675321/1‑150 (MQ=255)
                                                                       gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTaa                                                                            >  2:1417053/1‑150 (MQ=255)
                                                                       gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTATGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGGCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTTCGAAAATCCCCGCATCTTGTGAAACTGCGAGTaa                                                                            >  1:528527/1‑150 (MQ=255)
                                                                                ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                   >  1:936780/1‑150 (MQ=255)
                                                                                ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCAGTAATCAAGTTCACGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                   >  1:1253463/1‑150 (MQ=255)
                                                                                  aaaaaGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc                                                                 >  2:508392/1‑150 (MQ=255)
                                                                                     aagaagGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATc                                                              <  1:1702642/150‑1 (MQ=255)
                                                                                        aagGGGCGGATCAGCCGTAAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAg                                                           <  1:322699/150‑1 (MQ=255)
                                                                                         agGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAgg                                                          >  1:427005/1‑150 (MQ=255)
                                                                                            ggCGGATCAGCCGTCAATTCAGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTaa                                                       >  2:787942/1‑150 (MQ=255)
                                                                                               ggATCAGCCGTCAATTCTGCCGTAATCATGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGTTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACtt                                                    <  1:1476920/150‑1 (MQ=255)
                                                                                                  tCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTc                                                 <  2:388632/150‑1 (MQ=255)
                                                                                                     gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATg                                              >  1:1579882/1‑150 (MQ=255)
                                                                                                     gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATg                                              >  2:1524638/1‑150 (MQ=255)
                                                                                                      ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                             >  1:1286392/1‑150 (MQ=255)
                                                                                                      ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                             >  2:654788/1‑150 (MQ=255)
                                                                                                          cAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTat                                         >  1:470401/1‑150 (MQ=255)
                                                                                                           aaTTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatc                                        >  1:531799/1‑150 (MQ=255)
                                                                                                             ttCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                      <  2:675321/150‑1 (MQ=255)
                                                                                                                tGCCGTAATCTAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTACCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATccc                                   >  2:1373432/1‑150 (MQ=255)
                                                                                                                   cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                      >  2:1254560/1‑144 (MQ=255)
                                                                                                                   cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                      <  1:1254560/144‑1 (MQ=255)
                                                                                                                   cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaa                                >  1:1211704/1‑150 (MQ=255)
                                                                                                                      aaTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaaaaa                             >  2:568612/1‑150 (MQ=255)
                                                                                                                       ttCAAGTTCCCGTTGATGTTGAAAATCCCGGTCTGAAGATCGTGATTTTTTTTGATGTATGGAGATTGCGAAAATCGCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                            <  1:450089/149‑1 (MQ=255)
                                                                                                                       aTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                            >  1:1590166/1‑150 (MQ=255)
                                                                                                                        tCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACt                           >  2:1622576/1‑150 (MQ=255)
                                                                                                                          aaGTTCCCGTTGATGTTGAAAATCCCTGTAAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAAGCCTACATTTCATCCTGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                         <  2:322852/150‑1 (MQ=255)
                                                                                                                          aaGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                         <  1:1524638/150‑1 (MQ=255)
                                                                                                                           aGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATg                        <  1:984674/150‑1 (MQ=255)
                                                                                                                             ttCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcg                      >  2:514000/1‑150 (MQ=255)
                                                                                                                                 cGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt                  >  2:1691629/1‑150 (MQ=255)
                                                                                                                                    tGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg               <  2:471635/150‑1 (MQ=255)
                                                                                                                                      tcaaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa             >  1:1244313/7‑150 (MQ=255)
                                                                                                                                       caaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaaa            >  1:1171360/6‑150 (MQ=255)
                                                                                                                                        gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg           >  1:87970/1‑150 (MQ=255)
                                                                                                                                        gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg           >  2:451728/1‑150 (MQ=255)
                                                                                                                                        gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg           >  1:721853/1‑150 (MQ=255)
                                                                                                                                        aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg           >  2:1235901/5‑150 (MQ=255)
                                                                                                                                        aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg           >  2:1353396/5‑150 (MQ=255)
                                                                                                                                         aaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGCAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCGATCATCCCAAAAAACTATGCGCGGTTAGAAAGc          >  1:987442/4‑150 (MQ=255)
                                                                                                                                         aaaaaaaTCCCGATCTTCGGACCGGGATTTTTTGTGATGTATGGAGATTGCGAAAATCCCCGCATCGTGGGAAACTGCGCGTAACCCAACATTTCATCCAGGTAACTTTTCATGGCTATCCTCCCAACAAACTATGCGCGGGTAGAAAGc          >  1:329641/4‑150 (MQ=255)
                                                                                                                                             aaaTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCt      <  1:852320/150‑1 (MQ=255)
                                                                                                                                               aTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGTGGTTAGAAAGCGGCTAt    <  2:95552/150‑1 (MQ=255)
                                                                                                                                                 cccGGTCAGAAGATCGGTATTTTTTTTTATGTATGGAGATTGCGAAAATCCCAGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAATAACTATGCGCGGTTAGAAAGCGGCTATcg  <  1:687231/150‑1 (MQ=255)
                                                                                                                                                 cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg  >  1:993227/1‑150 (MQ=255)
                                                                                                                                                 cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg  >  1:751071/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ACTGCATCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCG  >  NC_000913/430357‑430651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: