Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,082,696 | G→C | 6.9% | Q22E (CAG→GAG) | fdoH ← | formate dehydrogenase‑O, Fe‑S subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,082,696 | 0 | G | C | 6.9% | 396.5 / 15.5 | 128 | Q22E (CAG→GAG) | fdoH | formate dehydrogenase‑O, Fe‑S subunit |
Reads supporting (aligned to +/- strand): ref base G (50/69); new base C (5/4); total (55/73) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-02 |
Only 100 of 132 total aligned reads displayed.
CATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCG > NC_000913/4082549‑4082845 | ctttGGGGTTGTCGTACACCCAAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 2:793757/148‑1 (MQ=255) cATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGGCTGAg > 1:150015/1‑150 (MQ=255) ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc > 2:822534/1‑150 (MQ=255) ttGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc < 2:146614/150‑1 (MQ=255) ttGGGGTTGTAGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGCCAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc < 1:228903/150‑1 (MQ=255) tGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcg > 1:1133031/1‑150 (MQ=255) tGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCAGAGgcg > 1:554220/1‑150 (MQ=255) gggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg < 2:21340/150‑1 (MQ=255) ggTTGTCGTACACCCCATTGTTTTTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg < 2:631068/150‑1 (MQ=255) ggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGACCACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCgg < 1:828991/150‑1 (MQ=255) ggTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCTGAGGAGCgg > 1:170089/1‑150 (MQ=255) gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggg < 2:758737/150‑1 (MQ=255) gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCggg < 1:1396146/150‑1 (MQ=255) tGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg > 1:263064/1‑150 (MQ=255) tGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg > 2:67426/1‑150 (MQ=255) gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGt > 2:418473/1‑150 (MQ=255) gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCggg > 1:615201/1‑147 (MQ=255) gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCggg < 2:615201/147‑1 (MQ=255) ccccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGt < 1:55298/150‑1 (MQ=255) ccccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCCCGGAGGCGCGGGGGTCAGAcccg > 2:369380/1‑148 (MQ=255) ccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCGGGGGTCAGACCGTTa > 1:204493/1‑150 (MQ=255) cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGACCGtttt > 2:359139/1‑148 (MQ=255) aattGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg < 2:699348/147‑1 (MQ=255) aTGTTATTGCCGACGGTATCGCGAATGTCGGTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTGCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑c < 1:97817/150‑1 (MQ=255) gTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTATTACTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGA‑cgc > 2:1033135/1‑150 (MQ=255) tATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCgg > 2:578304/1‑150 (MQ=255) tATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCG‑CACTTCTTCCTGGAAGTCCCGCGCCGGCGGCGCGGGGGTCAGAACGTTAGT‑CGCggc > 1:654054/1‑149 (MQ=37) gCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAacg > 2:919338/1‑150 (MQ=255) ccGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAacgc > 2:465885/1‑149 (MQ=255) gACGGCATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCCCGGGGGCGGGGGGGGCAGGCCGGTTGT‑CGCGGCacgccg > 1:1100230/1‑150 (MQ=21) gTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGCCCGTTAGT‑CGCGGCACGACGAATg > 2:496200/1‑150 (MQ=255) tATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGa < 1:962638/150‑1 (MQ=255) tCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑TGCGGAACGACGAATGata < 1:629132/150‑1 (MQ=255) tCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGata < 2:744549/150‑1 (MQ=255) cgcgAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGatat > 2:783917/1‑150 (MQ=255) gcgAATGTCGTTCCACTCTGAACACGCCACCTGACTGGCTTTACAGCCGATTCAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATTATATc < 2:1353599/150‑1 (MQ=255) aaTGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTTACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGTGGCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTg > 1:1089907/1‑150 (MQ=255) gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGGGCGGGGGTCAGACCGGTAGT‑CGCGGAACGACGAATGATATCTTgaga > 1:1362057/1‑150 (MQ=255) tCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGCCCGTTAGT‑CGCGGAACGACGAATGATATATTgagaa > 1:1041630/1‑149 (MQ=255) gTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTg < 1:919338/150‑1 (MQ=255) gTTCCACTCTCAACACCCCACCTGACAGGCTTTACACCCGATACAGGTGGTAACGTCGATGAGTATCGACACTTCTTCCTGTAAGTCCCGCGCCTGAGGCGCGGGGGTCGGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTg < 1:1045810/150‑1 (MQ=255) ttCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGa < 2:1133031/150‑1 (MQ=255) ccACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATa < 2:230739/150‑1 (MQ=255) aCTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGCCCGGTAGT‑CGCGGAACCACGAATGATATCTTGAGCTTGATAAg > 1:402379/1‑150 (MQ=37) ctctGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:57723/150‑1 (MQ=255) gAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata > 2:1030141/1‑150 (MQ=255) acacGCCACCTGACAGGCTTTACATCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATg < 2:1636756/150‑1 (MQ=255) gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgt > 2:70599/1‑150 (MQ=255) gCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCGCCGGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgt > 2:400639/1‑150 (MQ=255) ccACCTGTCAGGCTTTACAGCCGATACAGGTGGTTACGTCGATGAGTTTCGCCAGTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGTTTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTTAGATTGATAATCCATATgtcgtc < 2:304012/150‑1 (MQ=38) ccccTGACACGCTTTCCAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtct < 1:316145/148‑1 (MQ=255) ccTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTcc > 1:331809/1‑150 (MQ=255) cTGACAGGCGTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGACGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCg < 2:452923/150‑1 (MQ=255) cAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac < 1:999490/150‑1 (MQ=255) ggCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGGGGGGGTCAGACCGTTAGT‑CGCGTAACGACGAATTATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTacac < 2:1708990/150‑1 (MQ=255) gCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACAcc < 2:509276/150‑1 (MQ=255) gtttACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCt < 2:1249489/149‑1 (MQ=255) cTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCt < 1:418473/150‑1 (MQ=255) cTTTACAGCCGATACAGGTGGTAACGGCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGTGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCt < 2:81887/150‑1 (MQ=255) tccAGCCGATACAGGTGGTAACGTCGATGAGGTTCGCCACATCTACCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCtttt < 2:628523/148‑1 (MQ=255) cAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCCCCCCGGAGGCGCGGGGGGCAGACCGTTAGT‑AGCGGGACCACGAAATATATATTGAGATAGATAAGCCAAATGTCGTATACGTTAAACCatttaa > 2:602078/1‑148 (MQ=255) ccGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcaca < 2:49549/150‑1 (MQ=255) gatctcAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACAt < 1:438386/145‑1 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACAt > 2:1234733/1‑150 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTCCCACAt < 2:331809/150‑1 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAAAGATATATTGAGATTGATAAGCCATATGTAGTCTACGTTACACCTTTTAcacaaagata > 2:438386/1‑144 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCTCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaag < 2:554220/150‑1 (MQ=255) gtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagg < 1:822534/150‑1 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaagg < 1:304098/150‑1 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaagg < 1:359139/150‑1 (MQ=255) tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTTCCCCTTTTCCACATTGACAAGGAAGGACTTa > 1:716651/1‑150 (MQ=255) tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTa < 2:1700846/150‑1 (MQ=255) ggttGAGTTTCGGCACTTCTTCGTGGAAGTCCGGCGCCTGATGCGCGGGGGTCAGACCGTTAGT‑CGCGGATCGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTaa < 2:1509823/147‑1 (MQ=255) cGATGAGTTTCGCCTCTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTaa < 2:277832/150‑1 (MQ=255) cGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAAGGCTATCTTGAGAGTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTaa > 1:1033417/1‑150 (MQ=255) aTGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAAc > 1:896518/1‑150 (MQ=255) gAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTc < 2:231736/150‑1 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGTGGCGCGGGGGTCAGCCCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGGCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCgg < 1:1251819/150‑1 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGc < 1:793631/150‑1 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGc < 2:1100230/150‑1 (MQ=255) tCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCg < 2:1693136/150‑1 (MQ=255) cGCCACTTCTTCCTGGAAGTCCCCCCCCTGAGGCGCGGGGGTCAGACCGTTAGT‑AGCGGAACGACCAATGATATCTTTAGATTGATAAGCCCAAAGTCGTCACCGTTACACCTTTTACACAATAACAAAGAAGGACTTTAACTACCGCGt > 2:1387804/1‑150 (MQ=255) gCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTc < 2:1719684/150‑1 (MQ=255) ccACTTCTTCCTGGAAGTCCCGCGCCGGGGGCGCGGGGGTGAGAACGGTTGTAGGGGGAACG‑GGAATTATATTGTTTTATTTATTAAGCATATGTTGTATCCCTTTAACCGTTTGCACATTAACAAGGAAGGGCGTTAACTcccgggggt > 2:1303417/1‑143 (MQ=255) cttcttCCTGGAAGTCCCGCGCCTGCGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCg < 1:1420601/150‑1 (MQ=255) cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCg < 1:67426/150‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgt < 1:1535974/150‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtg < 1:1129615/150‑1 (MQ=255) ttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCCGACCGTTATT‑CGCGGAACGCCGAATGATATCTTGAGATTGATAAGCCATATTTCGTCTCCGTTACACCGTTTCCACATTAACAAGGAAGGACTTAAAAAACGGCGTAAGCGTGtt > 2:841243/1‑150 (MQ=255) ccTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCg < 2:1421560/150‑1 (MQ=255) gacccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCa > 2:1131394/5‑150 (MQ=255) cccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 2:803221/3‑150 (MQ=255) cccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 1:1313609/3‑150 (MQ=255) cccGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa > 1:401531/1‑150 (MQ=255) cgcCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa < 1:1387804/150‑1 (MQ=255) ccggAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATg < 1:1342051/147‑1 (MQ=255) ccTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATg > 1:332437/1‑150 (MQ=255) ccTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATg > 1:853056/1‑150 (MQ=255) gAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCg < 1:70599/150‑1 (MQ=255) gAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCg < 2:1712877/150‑1 (MQ=255) | CATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGT‑CGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCG > NC_000913/4082549‑4082845 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |