Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,093,463 A→G 8.2% S270S (AGT→AGC rhaD ← rhamnulose‑1‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,093,4630AG 8.2% 304.3 / 15.3 112S270S (AGT→AGCrhaDrhamnulose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (56/45);  new base G (3/6);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 113 total aligned reads displayed.

CGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCAG  >  NC_000913/4093315‑4093612
                                                                                                                                                    |                                                                                                                                                     
cGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCACTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCAc                                                                                                                                                      >  1:967758/1‑150 (MQ=255)
       aTGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGTTGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGGTTACCTGCATGACAAAGGCTTTGCGGATAATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAg                                                                                                                                               <  2:1497133/150‑1 (MQ=255)
       aTGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgct                                                                                                                                               <  1:1118342/150‑3 (MQ=255)
       aTGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgct                                                                                                                                               <  1:1360426/150‑3 (MQ=255)
        tGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTTGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgctg                                                                                                                                              <  1:988153/150‑4 (MQ=255)
        tGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgctg                                                                                                                                              <  1:1017403/150‑4 (MQ=255)
        tGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgctg                                                                                                                                              <  2:1541826/150‑4 (MQ=255)
         gcgcACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGTGCGCTGGCgctgt                                                                                                                                             <  1:513380/150‑5 (MQ=255)
            cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGTGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCg                                                                                                                                          >  2:1130198/1‑150 (MQ=255)
            cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCg                                                                                                                                          >  1:1648906/1‑150 (MQ=255)
            cACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGGTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCg                                                                                                                                          <  2:1152326/150‑1 (MQ=255)
                   agaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGcc                                                                                                                                   <  1:1580552/150‑1 (MQ=255)
                     aTTTCCGCACTGCTTCCAGTTCTGGCCACATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGa                                                                                                                                 <  2:128124/150‑1 (MQ=255)
                       ttCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGaaa                                                                                                                               >  2:1469598/1‑150 (MQ=255)
                            cACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTCCCTGCAGGACAAAGGCTTTGCGGATCCTGTTTGCTCCTTACAGCTCCCGAGCACTGGCGAGTGGCGTAACGCCGAAACGCtt                                                                                                                          >  1:433570/1‑150 (MQ=255)
                             acggCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATCCTCTTCGGGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTg                                                                                                                         <  1:1029723/147‑1 (MQ=255)
                                gCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCg                                                                                                                      <  2:51980/150‑1 (MQ=255)
                                 cTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGa                                                                                                                     >  2:64520/1‑150 (MQ=255)
                                  ttCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAg                                                                                                                    >  1:701104/1‑150 (MQ=255)
                                  ttCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAg                                                                                                                    <  2:1363017/150‑1 (MQ=255)
                                   tCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAgc                                                                                                                   >  1:1649407/1‑150 (MQ=255)
                                    ccAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAgcg                                                                                                                  <  2:1637043/150‑1 (MQ=255)
                                      aGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCt                                                                                                                >  2:838378/1‑150 (MQ=255)
                                         tCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATc                                                                                                             >  1:380618/1‑150 (MQ=255)
                                              ccAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAActc                                                                                                        <  1:1645838/150‑1 (MQ=255)
                                               cAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAActct                                                                                                       >  2:739567/1‑150 (MQ=255)
                                                aGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCtt                                                                                                      <  2:1590295/150‑1 (MQ=255)
                                                 gATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTc                                                                                                     <  1:1624982/150‑1 (MQ=255)
                                                    gggATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCATCACg                                                                                                  >  1:1688033/1‑150 (MQ=255)
                                                     ggATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACgg                                                                                                 >  1:65898/1‑150 (MQ=255)
                                                     ggATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACgg                                                                                                 >  2:237167/1‑150 (MQ=255)
                                                      gATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGc                                                                                                >  1:746928/1‑150 (MQ=255)
                                                        ttATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTg                                                                                              >  1:1099463/1‑150 (MQ=255)
                                                              cGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTc                                                                                        >  2:1240572/1‑150 (MQ=255)
                                                                  gCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGATACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGtt                                                                                    <  1:966974/150‑1 (MQ=255)
                                                                  gCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGtt                                                                                    <  1:1704729/150‑1 (MQ=255)
                                                                    tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTc                                                                                  >  2:29439/1‑150 (MQ=255)
                                                                    tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTc                                                                                  >  1:1430919/1‑150 (MQ=255)
                                                                    tGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTc                                                                                  >  1:1424212/1‑150 (MQ=255)
                                                                     gATACTCTTCGTGGGCGTCGGGGGTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCa                                                                                 <  1:1076535/150‑1 (MQ=255)
                                                                        aCTCTTCGTTGGCGTGGGGGTTTACCTGGAGGACATAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATGAACTCTTCACGGCTGATGGTCTGTTTCATgc                                                                              <  2:132391/150‑1 (MQ=255)
                                                                         ctctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCAGgcc                                                                             >  1:1039589/1‑150 (MQ=255)
                                                                           ctTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATgccgc                                                                           >  2:1693625/1‑150 (MQ=255)
                                                                              cGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCa                                                                        >  2:1102392/1‑150 (MQ=255)
                                                                               gTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCAt                                                                       >  2:426908/1‑150 (MQ=255)
                                                                               gTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCAt                                                                       <  2:701104/150‑1 (MQ=255)
                                                                               gTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTGGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCAt                                                                       >  2:1469396/1‑150 (MQ=255)
                                                                                tGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATc                                                                      <  1:237167/150‑1 (MQ=255)
                                                                                   gCGTCGGGGTTACCTGCATGACAAAGGCTTTGCGGATCAAGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAAt                                                                  >  1:1482502/1‑150 (MQ=255)
                                                                                       cGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATaaa                                                               >  2:1654052/1‑150 (MQ=255)
                                                                                         gggTTTACCTGCATGAGAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAAcc                                                             <  1:291533/150‑1 (MQ=255)
                                                                                         gggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAAcc                                                             >  2:1059968/1‑150 (MQ=255)
                                                                                         gggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAAcc                                                             <  1:430229/150‑1 (MQ=255)
                                                                                          ggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCt                                                            >  2:872060/1‑150 (MQ=255)
                                                                                          ggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCt                                                            >  1:702207/1‑150 (MQ=255)
                                                                                          ggTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCAGCGAATAAACCt                                                            >  1:263323/1‑150 (MQ=255)
                                                                                             ttACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                         <  1:728431/150‑1 (MQ=255)
                                                                                             ttACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCa                                                         >  2:1312824/1‑150 (MQ=255)
                                                                                              tACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCAc                                                        <  1:745720/150‑1 (MQ=255)
                                                                                               aCCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACt                                                       <  2:1430919/150‑1 (MQ=255)
                                                                                               aCCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACt                                                       <  2:1424212/150‑1 (MQ=255)
                                                                                                ccTGCATGACAAAGGCATTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGACGAGCGATATCAACTCTACACGGCTGAAGGTCTGATTCATGCAGCCCATCGAATAAACCTTCACTa                                                      >  1:239741/1‑150 (MQ=255)
                                                                                                  tGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAAt                                                    <  2:1649407/150‑1 (MQ=255)
                                                                                                   gCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATa                                                   >  1:1147824/1‑150 (MQ=255)
                                                                                                     aTGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACt                                                 <  2:309274/150‑1 (MQ=255)
                                                                                                        aCAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgt                                              <  2:167197/150‑1 (MQ=255)
                                                                                                        aCAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgt                                              >  2:703032/1‑150 (MQ=255)
                                                                                                        aCAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTtgt                                              <  1:1588621/150‑1 (MQ=255)
                                                                                                           aaGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCt                                           <  1:686110/150‑1 (MQ=255)
                                                                                                               cTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGCGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATTCCGCCAATCGAAAAAACCTTCACTAATACTTGTGCTGAtt                                       >  2:1119653/1‑150 (MQ=255)
                                                                                                               cTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGAtt                                       <  1:167403/150‑1 (MQ=255)
                                                                                                                tttGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGAttt                                      <  2:1648906/150‑1 (MQ=255)
                                                                                                                  tGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGAttttt                                    >  2:1444391/1‑150 (MQ=255)
                                                                                                                  tGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGGGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTACACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATTAACCTTCACTAATACTTGTGCTGAttttt                                    <  2:969887/150‑1 (MQ=255)
                                                                                                                  tGCGGATCATGTATGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGAttttt                                    <  2:409192/150‑1 (MQ=255)
                                                                                                                     ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTTGCGAGTGGCGTAAAGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATACACCTTCACTAATACTTGTGATGATTTTTCTg                                 >  2:314232/1‑150 (MQ=255)
                                                                                                                     ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGGCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGGTTCATGCCGCCAATCGAATAAACCTGAACTAATACTTGTGCTGATTTTTCTg                                 >  2:309530/1‑150 (MQ=255)
                                                                                                                     ggATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTg                                 >  1:840238/1‑150 (MQ=255)
                                                                                                                      gATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGc                                <  1:1469598/150‑1 (MQ=255)
                                                                                                                         cATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGgt                             <  2:670028/150‑1 (MQ=255)
                                                                                                                              ttGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGa                        <  2:33922/150‑1 (MQ=255)
                                                                                                                               tGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAt                       <  2:380618/150‑1 (MQ=255)
                                                                                                                                gCTCCTTACATCGCCTGCGCTCTGGCGAGTTGCGTATCGCCGAAACGCTTGCCTAGCGATATCAACTCTTCACGGCTGATGGTCTGTTTCTTGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAtt                      <  1:51080/150‑1 (MQ=255)
                                                                                                                                gCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAtt                      <  2:967758/150‑1 (MQ=255)
                                                                                                                                gCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGAtt                      <  1:1654052/150‑1 (MQ=255)
                                                                                                                                   ccTTACAGCGCCAGCGCACTGGCGAGTGGCGTATCGCCGAAACGCTTGCCGAGCGCTATCAACTCATCACGGCTGAGGGTCTGTTACCGTCCGCCCATCGAATAATCCTACACTAATACTTGTGCTGAATTATCAGCGGTGTCGATTaaa                   >  1:1101910/1‑150 (MQ=255)
                                                                                                                                     ttACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAAcc                 <  2:882467/150‑1 (MQ=255)
                                                                                                                                      tACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCg                >  2:1550040/1‑150 (MQ=255)
                                                                                                                                       cactcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGa               >  1:424430/5‑150 (MQ=255)
                                                                                                                                         ctcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAg             >  1:1451638/3‑150 (MQ=255)
                                                                                                                                         ctcgccagtgcGCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAg             >  1:614880/3‑150 (MQ=255)
                                                                                                                                         agcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAg             >  1:1434098/1‑150 (MQ=255)
                                                                                                                                         agcgccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAg             <  1:872060/150‑1 (MQ=255)
                                                                                                                                            gccagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGtt          >  2:471151/1‑150 (MQ=255)
                                                                                                                                              cagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTc        <  2:1231828/150‑1 (MQ=255)
                                                                                                                                              cagcgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCGGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTc        >  1:1623239/1‑150 (MQ=255)
                                                                                                                                              cagcgcACTGGCGAGTGGCGGAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCAAGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTc        <  2:89591/150‑1 (MQ=255)
                                                                                                                                                 cgcACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATc     >  2:959184/1‑150 (MQ=255)
                                                                                                                                                   cACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCa   >  2:1302765/1‑150 (MQ=255)
                                                                                                                                                    aCTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCTTCGGTTTAATcgac  <  1:1240572/150‑4 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCCAGCGCACTGGCGAGTGGCGTAACGCCGAAACGCTTGCCGAGCGCTATCAACTCTTCACGGCTGATGGTCTGTTTCATGCCGCCCATCGAATAAACCTTCACTAATACTTGTGCTGATTTTTCTGCGGTGTCGATTAAACCGAAGGTTTCATCCAG  >  NC_000913/4093315‑4093612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: