Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,527 T→A 5.1% intergenic (+31/+75) yiiM → / ← cpxA 6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5270TA 5.1% 362.0 / 5.8 118intergenic (+31/+75)yiiM/cpxA6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base T (60/52);  new base A (2/4);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 120 total aligned reads displayed.

ATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCC  >  NC_000913/4103378‑4103675
                                                                                                                                                     |                                                                                                                                                    
aTCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTAt                                                                                                                                                      >  2:1691307/1‑150 (MQ=255)
 tCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACACTATc                                                                                                                                                     <  2:445492/150‑1 (MQ=255)
     tatCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCg                                                                                                                                                 >  1:1598729/1‑150 (MQ=255)
          ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTGATAGCGTctta                                                                                                                                            <  1:1603534/150‑4 (MQ=255)
            gTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTGATAGCGTcttatc                                                                                                                                          <  2:1330223/150‑6 (MQ=255)
              ttACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAg                                                                                                                                        <  2:603003/150‑1 (MQ=255)
               tACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAgg                                                                                                                                       <  2:742476/150‑1 (MQ=255)
               tACTCTCCGCGGCCGGGGTATCGAAAAGCTGGACGCGGACGAGGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATGCGACGCTGATAGCGTCTTATCAgg                                                                                                                                       <  1:692076/150‑1 (MQ=255)
                aCTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGc                                                                                                                                      >  1:1057632/1‑150 (MQ=255)
                aCTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGACGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCTCATCAGGc                                                                                                                                      >  2:1557940/1‑150 (MQ=255)
                 ctctCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCa                                                                                                                                     >  2:827767/1‑150 (MQ=255)
                   gtcCGCTGCCGGGTTATCGAAATGGTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                                   <  2:620215/149‑1 (MQ=255)
                    tcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGGAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATcc                                                                                                                                  <  2:558888/150‑1 (MQ=255)
                    tcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATcc                                                                                                                                  >  1:174422/1‑150 (MQ=255)
                     ccGCTGCCGGGTTAGCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCt                                                                                                                                 <  1:536699/150‑1 (MQ=255)
                        cTGCCGGGGAATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCt                                                                                                                              <  2:224374/150‑1 (MQ=255)
                          gCCGGGTTAGCGACCTGCTGGACGCGGAGGATGCACAAGCGCCGCCTGAGCGGCAAGTTGGATGATTTTTCCCGCCGGGGCTGGGGATAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCa                                                                                                                            <  2:302485/150‑1 (MQ=255)
                            cGGGTTATCGATAAGCTGTACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCaaa                                                                                                                          <  1:1694365/150‑1 (MQ=255)
                               gTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGc                                                                                                                       <  1:285874/150‑1 (MQ=255)
                                 tATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                     <  1:1211340/150‑1 (MQ=255)
                                 tATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCg                                                                                                                     <  1:1226272/150‑1 (MQ=255)
                                    cGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGAt                                                                                                                  >  2:154289/1‑150 (MQ=255)
                                     gAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATg                                                                                                                 <  2:1441449/150‑1 (MQ=255)
                                        aaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcgg                                                                                                              <  2:1407758/150‑1 (MQ=255)
                                         aGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGAAAGATCGACGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcggc                                                                                                             <  2:1171827/150‑1 (MQ=255)
                                          gCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgcggcg                                                                                                            <  1:432134/150‑1 (MQ=255)
                                           cTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGt                                                                                                           >  1:1469678/1‑150 (MQ=255)
                                             ggACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaa                                                                                                         >  1:1710120/1‑150 (MQ=255)
                                                  cgGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGcc                                                                                                    <  2:579678/150‑1 (MQ=255)
                                                     aCGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTa                                                                                                 <  1:154289/150‑1 (MQ=255)
                                                      cGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCCTCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTAt                                                                                                >  2:1100241/1‑150 (MQ=255)
                                                      cGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTAt                                                                                                <  2:1320026/150‑1 (MQ=255)
                                                      cGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTCTGGGGAAAATAACCCCCGGAGTGTAGGCCAGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTAt                                                                                                >  2:101345/1‑150 (MQ=255)
                                                       gATGAAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATATGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATc                                                                                               <  1:1076418/150‑1 (MQ=255)
                                                        aTGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcc                                                                                              <  2:480138/150‑1 (MQ=255)
                                                        aTGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcc                                                                                              >  1:1121442/1‑150 (MQ=255)
                                                         tGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAACTGCCGGATGCGGCGTAAACGCCTTATcct                                                                                             >  1:1484941/1‑150 (MQ=255)
                                                           cAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCCTCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgc                                                                                           >  1:684870/1‑150 (MQ=255)
                                                            aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGTGGGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcc                                                                                          <  2:970393/150‑1 (MQ=255)
                                                             aaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcct                                                                                         >  1:45184/1‑150 (MQ=255)
                                                               aGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCctgcctgc                                                                                       <  1:827767/150‑1 (MQ=255)
                                                                  gcCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCaaa                                                                                    >  1:1583887/1‑150 (MQ=255)
                                                                        tGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                              >  1:911848/1‑150 (MQ=255)
                                                                             ggCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTa                                                                         <  2:1583887/150‑1 (MQ=255)
                                                                               cAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAAc                                                                       <  2:1150882/150‑1 (MQ=255)
                                                                                 aGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                              <  2:510627/141‑1 (MQ=255)
                                                                                 aGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                              >  1:510627/1‑141 (MQ=255)
                                                                                 aGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTc                                                                     >  1:1594102/1‑150 (MQ=255)
                                                                                   aTCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCg                                                                   >  1:1351075/1‑150 (MQ=255)
                                                                                    tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCTGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                                  <  1:252451/150‑1 (MQ=255)
                                                                                     cGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTTAACGCCTTATCCTGCCTGCAAATGCGATGTTTAACTCCGCt                                                                 >  1:1630476/1‑150 (MQ=255)
                                                                                     cGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCt                                                                 >  1:1608197/1‑150 (MQ=255)
                                                                                      gAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCtt                                                                >  1:1039738/1‑150 (MQ=255)
                                                                                      gAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCtt                                                                >  2:414505/1‑150 (MQ=255)
                                                                                       aaGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTta                                                               >  1:655515/1‑150 (MQ=255)
                                                                                         gATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTtata                                                             >  1:1687845/1‑150 (MQ=255)
                                                                                          aTTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATAc                                                            >  2:273773/1‑150 (MQ=255)
                                                                                           tttttCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACa                                                           <  1:602656/150‑1 (MQ=255)
                                                                                              ttCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCg                                                        >  2:183541/1‑150 (MQ=255)
                                                                                               tCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCgg                                                       <  2:666288/150‑1 (MQ=255)
                                                                                               tCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCgg                                                       >  1:367754/1‑150 (MQ=255)
                                                                                                 ccgccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCa                                                     >  1:1416718/1‑150 (MQ=255)
                                                                                                 ccgccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCa                                                     <  1:567971/150‑1 (MQ=255)
                                                                                                 ccgccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCa                                                     >  1:1409822/1‑150 (MQ=255)
                                                                                                     cgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCa                                                 <  2:520991/150‑1 (MQ=255)
                                                                                                      ggTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCaa                                                <  2:367754/150‑1 (MQ=255)
                                                                                                       gTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCaaa                                               >  1:525334/1‑150 (MQ=255)
                                                                                                         ggtgGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTATCTGCGCTTATACAGCGGCAACCAAATc                                             <  2:1563100/149‑1 (MQ=255)
                                                                                                          gtgGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATcc                                            >  2:1041179/1‑149 (MQ=255)
                                                                                                          gtgGGGAAAAAACCCCCCGGAGTGTCGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTAACCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATCCGTCGGCAACCAAATCa                                            >  2:1041183/1‑150 (MQ=37)
                                                                                                           tgGGGAAAATAACACCCGGAGTGTAGGCATGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCTGCAACCAAATCAc                                           >  2:15917/1‑150 (MQ=255)
                                                                                                            ggggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCAcc                                          <  2:162439/150‑1 (MQ=255)
                                                                                                             gggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCa                                         >  2:1596091/1‑150 (MQ=255)
                                                                                                              ggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAg                                        >  1:1690975/1‑150 (MQ=255)
                                                                                                              ggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAg                                        >  1:1019608/1‑150 (MQ=255)
                                                                                                               gAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGc                                       <  2:1710120/150‑1 (MQ=255)
                                                                                                                 aaaTAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCg                                     >  2:339052/1‑150 (MQ=255)
                                                                                                                       cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAcc                               >  1:257134/1‑150 (MQ=255)
                                                                                                                       cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAcc                               >  1:965332/1‑150 (MQ=255)
                                                                                                                          ccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGAGTAAACGGCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGcc                            <  2:252706/150‑1 (MQ=255)
                                                                                                                            ggAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCa                          >  1:999160/1‑150 (MQ=255)
                                                                                                                                tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGTgg                      >  1:386642/1‑150 (MQ=255)
                                                                                                                                tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg                      >  2:985350/1‑150 (MQ=255)
                                                                                                                                tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg                      <  1:414505/150‑1 (MQ=255)
                                                                                                                                tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg                      >  2:489154/1‑150 (MQ=255)
                                                                                                                                 ggatgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggg                     >  1:704331/5‑150 (MQ=38)
                                                                                                                                 ggatgCCTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCggg                     <  2:704331/146‑1 (MQ=38)
                                                                                                                                    ggCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCACTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTg                  >  2:1634254/1‑150 (MQ=255)
                                                                                                                                      ccTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc                >  2:907039/1‑150 (MQ=38)
                                                                                                                                      ccTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc                >  1:886892/1‑150 (MQ=255)
                                                                                                                                       cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct               <  2:1594102/150‑1 (MQ=255)
                                                                                                                                       cTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct               >  2:865815/1‑150 (MQ=255)
                                                                                                                                         gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc             <  2:1416718/150‑1 (MQ=255)
                                                                                                                                         gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc             >  1:289456/1‑150 (MQ=255)
                                                                                                                                         gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc             <  2:676851/150‑1 (MQ=255)
                                                                                                                                         gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc             <  1:1451005/150‑1 (MQ=255)
                                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttct            >  1:29086/1‑150 (MQ=255)
                                                                                                                                           tAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTg           <  2:1598729/150‑1 (MQ=255)
                                                                                                                                              gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCt        >  2:923155/1‑150 (MQ=255)
                                                                                                                                              gACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCt        <  2:971775/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGCCTTCACCC  >  NC_000913/4103378‑4103675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: