Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 430,509 A→T 13.4% intergenic (+33/‑96) secF → / → yajD SecYEG protein translocase auxillary subunit/HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913430,5090AT13.4% 206.4 / 17.3 86intergenic (+33/‑96)secF/yajDSecYEG protein translocase auxillary subunit/HNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base A (42/32);  new base T (6/6);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAA  >  NC_000913/430363‑430658
                                                                                                                                                  |                                                                                                                                                      
gcTTCCATCTATGTGGCATCTGCGTTGGCGCTGAAACTGGGTATGAAGCGCGATCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCagaa                                                                                                                                                     <  1:1455515/149‑1 (MQ=255)
     cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGCAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCCGCCGTCAATTCTGCCGTAATCAAGTTACCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                                >  1:95552/1‑150 (MQ=255)
     cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                                >  2:1280649/1‑150 (MQ=255)
      aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                               >  2:1702642/1‑150 (MQ=255)
      aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                               <  1:1548820/150‑1 (MQ=255)
       tCTATGTGGCATCTGCGCTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCggg                                                                                                                                              <  2:164162/150‑1 (MQ=255)
         tATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAt                                                                                                                                           >  2:984674/1‑150 (MQ=255)
            gtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttt                                                                                                                                        >  1:503699/1‑150 (MQ=255)
              ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                                                                                                      >  1:593712/1‑150 (MQ=255)
              ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                                      <  2:1710169/150‑3 (MQ=255)
              ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                                      <  2:1648435/150‑3 (MQ=255)
               gCATGTGCGTTGGCTCTGAACCTGGGAATGAAGCGCGAACACATTTTGCAGCAGAAAGTGGAAAAAGAAGTGGCGGATCAGCCGTCATTTCTGCCGTATTCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                                     <  2:1673434/150‑4 (MQ=255)
               gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                                     <  2:1188811/150‑1 (MQ=255)
               gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                                     <  2:657523/150‑4 (MQ=255)
               gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGCTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                                     <  1:1596409/150‑1 (MQ=255)
                cATCTTCGTTTGCTCTGATACTGGGTATGAAGCGCTAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTTATGTTGAAAATCCCGGTCAGAAGATCGTGAtttttttt                                                                                                                                    <  1:590666/150‑1 (MQ=255)
                cATCTGCGTTGGCTCTGAAACTGGGTATGATGCGCTAACACATGTTGCAGCAGAACGCGGAAAAAGAAGTGTCGTATCAGCCGTCAACTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttttt                                                                                                                                    <  1:266173/150‑1 (MQ=255)
                cATCTGCGTTGGCTCTGAAACTGGGTATGAACCGCGAAGAAATGTTGCAGGAGAAACTGGAAAAAGAACTGGCGGATCAGCCGGCAATTCTTCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaac                                                                                                                                    <  2:776071/150‑5 (MQ=255)
                 aTCTGCGTTGGCTCTGAAACTGTGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTg                                                                                                                                   <  1:1553515/150‑1 (MQ=255)
                              ctGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATCTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGAtt                                                                                                                      >  2:450089/1‑150 (MQ=255)
                                taaaCTGGGTATTAAGCGCGAACACATGTTTCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCAGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                                    <  2:321371/149‑1 (MQ=255)
                                gAAACTGGGTATGAAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGGCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                                    <  1:1203356/150‑1 (MQ=255)
                                 aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATATTTTTTGATTTATGGAGATTGCg                                                                                                                   >  2:687231/1‑150 (MQ=255)
                                 aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGGTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCg                                                                                                                   <  2:1636866/150‑1 (MQ=255)
                                    cTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGaaa                                                                                                                >  1:1083918/1‑150 (MQ=255)
                                          aTGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATcccc                                                                                                          <  2:577605/150‑1 (MQ=255)
                                           tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGTGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTATTATTGATGTATGGAGATTGCGAAAATCCCCg                                                                                                         >  1:1167675/1‑150 (MQ=255)
                                           tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTGATGTATGGAGATTGCGAAAATCCCCGc                                                                                                        <  1:1596794/150‑1 (MQ=255)
                                            gAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCACGc                                                                                                        >  1:95204/1‑150 (MQ=255)
                                             aaGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCACCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCa                                                                                                       <  2:1690147/150‑1 (MQ=255)
                                               gcgcGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATc                                                                                                     >  1:835007/1‑150 (MQ=255)
                                                   gcacacCTGTGGCCGCAGAAAGGGCCAAAAGAAGGGGCGGAGCAGCCGTCAAGGCTGCCGTAATCAAGTGCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                                 <  1:1333216/148‑1 (MQ=255)
                                                    aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                                 <  2:439891/150‑1 (MQ=255)
                                                    aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTggg                                                                                                >  1:1139812/1‑150 (MQ=255)
                                                     acacATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGa                                                                                               <  1:1175635/150‑1 (MQ=255)
                                                        caTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAAc                                                                                            <  1:519590/150‑1 (MQ=255)
                                                         aTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACt                                                                                           >  2:852320/1‑150 (MQ=255)
                                                           gTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgc                                                                                         <  1:669501/150‑1 (MQ=255)
                                                            ttGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgcg                                                                                        <  2:593712/150‑1 (MQ=255)
                                                              gcagcaGAAAGTGGAAAAAGAAGGGGCGGATCAGCCATCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGcgcg                                                                                      >  2:188755/1‑150 (MQ=255)
                                                                agcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTa                                                                                    >  1:675321/1‑150 (MQ=255)
                                                                 gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTaa                                                                                   >  2:1417053/1‑150 (MQ=255)
                                                                 gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTATGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGGCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTTCGAAAATCCCCGCATCTTGTGAAACTGCGAGTaa                                                                                   >  1:528527/1‑150 (MQ=255)
                                                                          ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                          >  1:936780/1‑150 (MQ=255)
                                                                          ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCAGTAATCAAGTTCACGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                          >  1:1253463/1‑150 (MQ=255)
                                                                            aaaaaGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc                                                                        >  2:508392/1‑150 (MQ=255)
                                                                               aagaagGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATc                                                                     <  1:1702642/150‑1 (MQ=255)
                                                                                  aagGGGCGGATCAGCCGTAAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAg                                                                  <  1:322699/150‑1 (MQ=255)
                                                                                   agGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAgg                                                                 >  1:427005/1‑150 (MQ=255)
                                                                                      ggCGGATCAGCCGTCAATTCAGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTaa                                                              >  2:787942/1‑150 (MQ=255)
                                                                                         ggATCAGCCGTCAATTCTGCCGTAATCATGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGTTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACtt                                                           <  1:1476920/150‑1 (MQ=255)
                                                                                            tCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTc                                                        <  2:388632/150‑1 (MQ=255)
                                                                                               gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATg                                                     >  1:1579882/1‑150 (MQ=255)
                                                                                               gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATg                                                     >  2:1524638/1‑150 (MQ=255)
                                                                                                ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                                    >  2:654788/1‑150 (MQ=255)
                                                                                                ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                                    >  1:1286392/1‑150 (MQ=255)
                                                                                                    cAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTat                                                >  1:470401/1‑150 (MQ=255)
                                                                                                     aaTTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatc                                               >  1:531799/1‑150 (MQ=255)
                                                                                                       ttCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                             <  2:675321/150‑1 (MQ=255)
                                                                                                          tGCCGTAATCTAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTACCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATccc                                          >  2:1373432/1‑150 (MQ=255)
                                                                                                             cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                             >  2:1254560/1‑144 (MQ=255)
                                                                                                             cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                             <  1:1254560/144‑1 (MQ=255)
                                                                                                             cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaa                                       >  1:1211704/1‑150 (MQ=255)
                                                                                                                aaTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaaaaa                                    >  2:568612/1‑150 (MQ=255)
                                                                                                                 ttCAAGTTCCCGTTGATGTTGAAAATCCCGGTCTGAAGATCGTGATTTTTTTTGATGTATGGAGATTGCGAAAATCGCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                   <  1:450089/149‑1 (MQ=255)
                                                                                                                 aTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                   >  1:1590166/1‑150 (MQ=255)
                                                                                                                  tCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACt                                  >  2:1622576/1‑150 (MQ=255)
                                                                                                                    aaGTTCCCGTTGATGTTGAAAATCCCTGTAAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAAGCCTACATTTCATCCTGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                                <  2:322852/150‑1 (MQ=255)
                                                                                                                    aaGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                                <  1:1524638/150‑1 (MQ=255)
                                                                                                                     aGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATg                               <  1:984674/150‑1 (MQ=255)
                                                                                                                       ttCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcg                             >  2:514000/1‑150 (MQ=255)
                                                                                                                           cGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt                         >  2:1691629/1‑150 (MQ=255)
                                                                                                                              tGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg                      <  2:471635/150‑1 (MQ=255)
                                                                                                                                tcaaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa                    >  1:1244313/7‑150 (MQ=255)
                                                                                                                                 caaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaaa                   >  1:1171360/6‑150 (MQ=255)
                                                                                                                                  gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                  >  1:721853/1‑150 (MQ=255)
                                                                                                                                  gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                  >  2:451728/1‑150 (MQ=255)
                                                                                                                                  gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                  >  1:87970/1‑150 (MQ=255)
                                                                                                                                  aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                  >  2:1353396/5‑150 (MQ=255)
                                                                                                                                  aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                  >  2:1235901/5‑150 (MQ=255)
                                                                                                                                   aaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGCAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCGATCATCCCAAAAAACTATGCGCGGTTAGAAAGc                 >  1:987442/4‑150 (MQ=255)
                                                                                                                                   aaaaaaaTCCCGATCTTCGGACCGGGATTTTTTGTGATGTATGGAGATTGCGAAAATCCCCGCATCGTGGGAAACTGCGCGTAACCCAACATTTCATCCAGGTAACTTTTCATGGCTATCCTCCCAACAAACTATGCGCGGGTAGAAAGc                 >  1:329641/4‑150 (MQ=255)
                                                                                                                                       aaaTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCt             <  1:852320/150‑1 (MQ=255)
                                                                                                                                         aTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGTGGTTAGAAAGCGGCTAt           <  2:95552/150‑1 (MQ=255)
                                                                                                                                           cccGGTCAGAAGATCGGTATTTTTTTTTATGTATGGAGATTGCGAAAATCCCAGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAATAACTATGCGCGGTTAGAAAGCGGCTATcg         <  1:687231/150‑1 (MQ=255)
                                                                                                                                           cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg         >  1:751071/1‑150 (MQ=255)
                                                                                                                                           cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg         >  1:993227/1‑150 (MQ=255)
                                                                                                                                                  agaagaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTGAGAAAGCGGCTATCGCGaaaaa  >  2:1483652/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TCTTCCATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAA  >  NC_000913/430363‑430658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: