Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,110,721 A→T 9.3% intergenic (+36/+19) cdh → / ← tpiA CDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,110,7210AT 9.3% 309.6 / 14.4 116intergenic (+36/+19)cdh/tpiACDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (53/52);  new base T (9/2);  total (62/54)
Fisher's exact test for biased strand distribution p-value = 5.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTT  >  NC_000913/4110575‑4110868
                                                                                                                                                  |                                                                                                                                                   
cGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATc                                                                                                                                                  <  1:1346163/150‑1 (MQ=255)
  gCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTCAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCg                                                                                                                                                <  1:254011/150‑1 (MQ=255)
    agagTGATAAATCTTTTGTATTGATGGCGACACAAGTAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGTTTCATCAGTGTGAGATTTTGCGTTAATGCGAAGAGTTAATGATAGTAAGTGCCGGATATGAAATCAGGc                                                                                                                                              <  2:312692/150‑1 (MQ=255)
      agTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATCCGGCAc                                                                                                                                            <  1:1087509/150‑1 (MQ=255)
      agTGATAACTCATTTGTATTGCTGGCGACACAAAGATATCTACTGACGCTTAACCGTGCTTCAGCCGAGTATATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCAc                                                                                                                                            <  1:198230/150‑1 (MQ=255)
        tGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCTAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCt                                                                                                                                          >  1:1354343/1‑150 (MQ=255)
        tGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCt                                                                                                                                          <  1:1280152/150‑1 (MQ=255)
         gATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTg                                                                                                                                         >  2:704875/1‑150 (MQ=255)
         gATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTg                                                                                                                                         >  2:134575/1‑150 (MQ=255)
           tAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTc                                                                                                                                       >  2:1526235/1‑150 (MQ=255)
             aCTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAg                                                                                                                                     >  2:408641/1‑150 (MQ=255)
                cATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACt                                                                                                                                  <  2:1193314/150‑1 (MQ=255)
                cATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGATTAAGCCGAGGAAATTCAGGATCATCAGTGTGAGAATTTGCGATAAGGCGAAGAGTTAAGGAAAGAAAGTGCCGCATATGAAATCCGGCACCTCTCAGACt                                                                                                                                  >  1:1655348/1‑150 (MQ=255)
                 aTTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACtt                                                                                                                                 >  2:515518/1‑150 (MQ=255)
                  tttGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTa                                                                                                                                >  1:544092/1‑150 (MQ=255)
                     gTATTGCTGGCGACACAACGAAATCTACTGCCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGc                                                                                                                             >  2:448214/1‑150 (MQ=255)
                     gTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGc                                                                                                                             >  1:968829/1‑150 (MQ=255)
                        ttGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTg                                                                                                                          <  2:454659/150‑1 (MQ=255)
                         tGCTGGCGACCCAACGAAATCTACTGACGCTTAACCGTTCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGt                                                                                                                         >  2:1159586/1‑150 (MQ=255)
                         tGCTGGCGACACAACTAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGt                                                                                                                         <  1:613119/150‑1 (MQ=255)
                         tGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGt                                                                                                                         <  1:744217/150‑1 (MQ=255)
                          gCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGtt                                                                                                                        >  2:321063/1‑150 (MQ=255)
                          gCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGtt                                                                                                                        <  2:622216/150‑1 (MQ=255)
                              gCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGc                                                                                                                    <  1:788373/150‑1 (MQ=255)
                               cGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGcc                                                                                                                   <  2:618035/150‑1 (MQ=255)
                               cGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGcc                                                                                                                   >  1:7204/1‑150 (MQ=255)
                                gACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCg                                                                                                                  >  1:1018775/1‑150 (MQ=255)
                                gACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCg                                                                                                                  <  1:1501148/150‑1 (MQ=255)
                                       cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGc                                                                                                           <  1:1526235/150‑1 (MQ=255)
                                       cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGGTAAGGAAAGTAAGGGCCGGATAGGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGc                                                                                                           <  1:131227/150‑1 (MQ=255)
                                        gAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAATTTCAGGATCATCAGTGTGAGAATTTGCGTTAAGGCGAAGAGTTAATGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCa                                                                                                          <  1:995456/150‑1 (MQ=255)
                                                  gACGCTTAACCGTGCTTCCGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGa                                                                                                >  1:1047481/1‑150 (MQ=255)
                                                  gACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGa                                                                                                >  2:2667/1‑150 (MQ=255)
                                                    cGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCAGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTTTTTAGCCGCTTCTTCAGCTTTAACGAtt                                                                                              >  1:297414/1‑150 (MQ=255)
                                                       ttAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACt                                                                                           <  1:408641/150‑1 (MQ=255)
                                                        tAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTg                                                                                          <  1:1082041/150‑1 (MQ=255)
                                                        tAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCATTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTg                                                                                          >  2:939672/1‑150 (MQ=255)
                                                         aaCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGc                                                                                         >  1:787366/1‑150 (MQ=255)
                                                             gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGTATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAg                                                                                     <  2:623817/150‑1 (MQ=255)
                                                             gTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAg                                                                                     >  1:1450312/1‑150 (MQ=255)
                                                              tGCTTCCGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAAGATTACTGCGAAgg                                                                                    >  1:547971/1‑150 (MQ=255)
                                                              tGCTTCAGCCGAGGAAATTCAGGATCCTCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAgg                                                                                    <  2:1529903/150‑1 (MQ=255)
                                                              tGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAgg                                                                                    >  1:647490/1‑150 (MQ=255)
                                                                  tCAGCCGAGGAAATTCAGGATCATCAGTGGGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTc                                                                                <  1:1533844/150‑1 (MQ=255)
                                                                   cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCa                                                                               <  2:623242/150‑1 (MQ=255)
                                                                      ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCt                                                                            <  1:617795/150‑1 (MQ=255)
                                                                      ccGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCATTAACGATTACTGCGAAGGCGTCAggt                                                                            >  2:234742/1‑148 (MQ=255)
                                                                             aaTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCagag                                                                     <  2:1018775/150‑1 (MQ=255)
                                                                              aTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAgaga                                                                    >  2:441842/1‑150 (MQ=255)
                                                                              aTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACATGTCAGACTTATGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAgaga                                                                    >  2:871880/1‑150 (MQ=255)
                                                                               ttCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGaa                                                                   >  1:109679/1‑150 (MQ=255)
                                                                                 cAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGc                                                                 >  1:1102329/1‑150 (MQ=255)
                                                                                 cAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGc                                                                 <  2:576244/150‑1 (MQ=255)
                                                                                   ggATCATCAGTGTGAGATTTTGCGTTACGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcac                                                               <  2:193256/150‑1 (MQ=255)
                                                                                   ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcac                                                               >  2:1600252/1‑150 (MQ=255)
                                                                                   ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcac                                                               <  1:999747/150‑1 (MQ=255)
                                                                                     atcatcAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcacca                                                             >  2:548325/1‑150 (MQ=255)
                                                                                       catcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCaccacc                                                           <  1:1154151/150‑1 (MQ=255)
                                                                                           aGTGTGAGATTTTGCGTTAAGGCGATGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCAGGCACCTGTCAGACTTAAGCCTGTATAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCaccaacc                                                       >  1:1451891/1‑150 (MQ=255)
                                                                                           aGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCaccaacc                                                       <  1:1590806/150‑1 (MQ=255)
                                                                                             tgtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAg                                                     >  1:1326495/1‑150 (MQ=255)
                                                                                              gtgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgc                                                    >  2:1103406/1‑150 (MQ=255)
                                                                                              gtgAGATTATGCGTTAAGGCGAAGCGTTACGGACAGTACGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgc                                                    <  2:180015/150‑1 (MQ=255)
                                                                                               tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcg                                                   <  2:1225380/150‑1 (MQ=255)
                                                                                               tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcg                                                   >  1:352538/1‑150 (MQ=255)
                                                                                                 agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcgcg                                                 <  1:1289179/150‑1 (MQ=255)
                                                                                                 agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcgcg                                                 >  2:385657/1‑150 (MQ=255)
                                                                                                 agaTTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAATACGGCACCTGTCAGACTTAAGCCGGTTTATCCGCATAAGCAGCTTTTACGATTAAGGCGAAAGCGTCAGCGTTAAGAGAAGCCCCACCAACCAgcgcgc                                                >  2:795211/1‑150 (MQ=255)
                                                                                                    ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTCTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCg                                              >  2:959936/1‑150 (MQ=255)
                                                                                                      ttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTc                                            >  2:589135/1‑150 (MQ=255)
                                                                                                          gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGata                                        <  2:691367/150‑1 (MQ=255)
                                                                                                          gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGata                                        >  1:599667/1‑150 (MQ=255)
                                                                                                          gTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGata                                        >  1:605800/1‑150 (MQ=255)
                                                                                                           ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGatat                                       <  1:2667/150‑1 (MQ=255)
                                                                                                           ttAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGatat                                       <  1:748138/150‑1 (MQ=255)
                                                                                                            tAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATc                                      >  1:1263331/1‑150 (MQ=255)
                                                                                                               tgctaaGAGTTAAGGAATGTAAGTGCCTTTTATGAAATGCGGCACCTGTCAGACTTAAGCCTTTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGTAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCGGTCGATATCCgg                                   <  2:1655348/146‑1 (MQ=255)
                                                                                                                gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGc                                  <  1:515518/150‑1 (MQ=255)
                                                                                                                  gAAGAGGTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTg                                <  1:321063/150‑1 (MQ=255)
                                                                                                                    agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAg                              <  1:939672/150‑1 (MQ=255)
                                                                                                                     gagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGc                             <  2:599667/150‑1 (MQ=255)
                                                                                                                     gagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGc                             <  2:605800/150‑1 (MQ=255)
                                                                                                                          aaGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACa                        >  2:921221/1‑150 (MQ=255)
                                                                                                                            ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGt                      <  2:1047481/150‑1 (MQ=255)
                                                                                                                              ctgacaggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTc                    >  2:986114/8‑150 (MQ=255)
                                                                                                                              ctgacaggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTc                    >  2:154606/8‑150 (MQ=255)
                                                                                                                               aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCt                   <  1:468110/150‑1 (MQ=255)
                                                                                                                                 gtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGCAGCACCACCAACCCGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGc                 >  1:496804/1‑150 (MQ=255)
                                                                                                                                  taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTGCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCa                <  1:1213047/150‑1 (MQ=255)
                                                                                                                                    ggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGc              >  2:1282541/2‑150 (MQ=255)
                                                                                                                                     gtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGGACCGCCATCCAGCGCGCCGTCGATATCCGGCTGAGCAAAGAGTTCTGCAGCg             >  1:293654/1‑150 (MQ=255)
                                                                                                                                     gtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg             >  1:455958/1‑150 (MQ=255)
                                                                                                                                     gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCCCCCACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg             >  1:731446/1‑150 (MQ=255)
                                                                                                                                      tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGt            >  2:403789/1‑150 (MQ=255)
                                                                                                                                      tGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCTAACAGTTCTGCCGCGt            >  2:396958/1‑150 (MQ=255)
                                                                                                                                       gCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGtt           >  1:1168048/1‑150 (MQ=255)
                                                                                                                                       gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGtt           >  1:1007970/1‑150 (MQ=255)
                                                                                                                                          ggATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGa        <  2:1263331/150‑1 (MQ=255)
                                                                                                                                               tGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGt   >  1:643119/1‑150 (MQ=255)
                                                                                                                                                gAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGtt  <  2:1450312/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCGTT  >  NC_000913/4110575‑4110868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: