Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,114,537 G→T 6.6% intergenic (‑65/+32) fpr ← / ← glpX ferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,5370GT 6.6% 435.5 / 11.5 143intergenic (‑65/+32)fpr/glpXferredoxin‑NADP reductase; flavodoxin reductase/fructose 1,6‑bisphosphatase II
Reads supporting (aligned to +/- strand):  ref base G (71/61);  new base T (7/3);  total (79/64)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATAT  >  NC_000913/4114391‑4114684
                                                                                                                                                  |                                                                                                                                                   
cGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCATTTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAg                                                                                                                                                  <  2:256545/150‑1 (MQ=255)
      cgggAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTgg                                                                                                                                            <  2:723309/147‑1 (MQ=255)
      cGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTgg                                                                                                                                            <  2:1689034/150‑1 (MQ=255)
        tGAACGGTGAGACTAAACAGGGCGGCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGGTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGaa                                                                                                                                          <  1:1289328/150‑1 (MQ=255)
         gAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAg                                                                                                                                         <  1:1268887/150‑1 (MQ=255)
         gAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAg                                                                                                                                         >  1:760919/1‑150 (MQ=255)
          aaCGGTGAGACTAAACAGGGCGTCTGTCCAGTTCTGCACTTTAGTGACTATTCCTGTTACCCAATCAGCCATCTTTATCTCCTGTATAGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaatt                                                                                                                                        <  1:282192/150‑3 (MQ=255)
          aaCGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaatt                                                                                                                                        <  1:1209076/150‑3 (MQ=255)
          aaCGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAgg                                                                                                                                        <  1:1159519/150‑1 (MQ=255)
           aCGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAATGACTGGAAGGc                                                                                                                                       >  2:1578714/1‑150 (MQ=255)
             ggTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTc                                                                                                                                     <  1:976825/150‑1 (MQ=255)
             ggTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCCTCGTTCTCCGAACAAAAAAATTCCAGTCCTTCGGGACTGGaattttt                                                                                                                                     <  1:1119755/150‑6 (MQ=255)
                 agaCTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATc                                                                                                                                 >  1:1426258/1‑150 (MQ=255)
                  gaCTAATCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCg                                                                                                                                <  1:583126/150‑1 (MQ=255)
                    cTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGat                                                                                                                              >  2:20871/1‑150 (MQ=255)
                     tAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTACGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatc                                                                                                                             >  2:1053618/1‑150 (MQ=255)
                      aaaCAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGatca                                                                                                                            <  1:691299/150‑1 (MQ=255)
                              cggcGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAaatcaatc                                                                                                                    <  2:1426156/147‑1 (MQ=255)
                              cGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAaatcaatc                                                                                                                    <  1:20871/150‑1 (MQ=255)
                                  ggTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCagag                                                                                                                <  1:201958/150‑1 (MQ=255)
                                   gTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAgg                                                                                                               >  1:475758/1‑150 (MQ=255)
                                   gTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAgg                                                                                                               >  2:651464/1‑150 (MQ=255)
                                     ccAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGAt                                                                                                             >  1:10483/1‑150 (MQ=255)
                                      cAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGAtg                                                                                                            <  2:1469967/150‑1 (MQ=255)
                                        gTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGAtgtg                                                                                                          <  2:713986/150‑1 (MQ=255)
                                         ttCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGc                                                                                                         <  2:548959/150‑1 (MQ=255)
                                           cTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCAc                                                                                                       <  2:112334/150‑1 (MQ=255)
                                                 tttAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCAt                                                                                                 >  1:786501/1‑150 (MQ=255)
                                                   tAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCAttt                                                                                               >  1:428178/1‑150 (MQ=255)
                                                     gTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTcc                                                                                             >  1:1235837/1‑150 (MQ=255)
                                                      tGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCg                                                                                            <  2:1695126/150‑1 (MQ=255)
                                                        aCTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCggg                                                                                          <  2:220642/150‑1 (MQ=255)
                                                        aCTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCggg                                                                                          <  2:308518/150‑1 (MQ=255)
                                                        aCTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCggg                                                                                          <  2:349411/150‑1 (MQ=255)
                                                        aCTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCggg                                                                                          >  1:886817/1‑150 (MQ=255)
                                                        aCTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCggg                                                                                          <  2:1424721/150‑1 (MQ=255)
                                                         cTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGt                                                                                         >  2:648289/1‑150 (MQ=255)
                                                           ttGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCtt                                                                                      >  1:133688/1‑150 (MQ=255)
                                                             gCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCttt                                                                                     <  2:1514259/150‑1 (MQ=255)
                                                             gCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCttt                                                                                     <  2:50481/150‑1 (MQ=255)
                                                              ccTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTg                                                                                    >  1:1658799/1‑150 (MQ=255)
                                                              ccTGTTACCCAATCAGCCATGCTTTTCACCTGTTTTGAATGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGGGCACCTGCATTTCCGGGTCTTTg                                                                                    <  2:1215717/150‑1 (MQ=255)
                                                                 gTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGa                                                                                 <  1:1175673/150‑1 (MQ=255)
                                                                  ttACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGAt                                                                                <  2:760919/150‑1 (MQ=255)
                                                                   tACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATc                                                                               <  2:848275/150‑1 (MQ=255)
                                                                     cccAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGATGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGTGATCCa                                                                             <  1:860934/150‑1 (MQ=255)
                                                                             gCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtgg                                                                     >  2:134761/1‑150 (MQ=255)
                                                                             gCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtgg                                                                     >  2:745127/1‑150 (MQ=255)
                                                                              ccATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtgga                                                                    >  2:380148/1‑150 (MQ=255)
                                                                               cATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGATCAAAAAAATTCCAGTCCCTAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtggat                                                                   >  1:1149278/1‑150 (MQ=255)
                                                                                aTGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtggatg                                                                  <  2:465461/150‑1 (MQ=255)
                                                                                  gTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGtggatgga                                                                >  1:910083/1‑150 (MQ=255)
                                                                                        ctcCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAAt                                                          <  2:1476864/150‑1 (MQ=255)
                                                                                         tcCTGTTTTGATTTACTTGCCTTATCTTCGTTCTCCGAACAAAAATATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATg                                                         <  2:549262/150‑1 (MQ=255)
                                                                                             gTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATgcggc                                                     <  1:144812/150‑1 (MQ=255)
                                                                                                 tgattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAAt                                                 >  2:398224/1‑150 (MQ=255)
                                                                                                  gattgaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATg                                                <  2:10483/150‑1 (MQ=255)
                                                                                                      gaCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCCAAGGACAGGAAGGCTAAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTACGGGTCTTTGCGATACAGATAGTGGATGGACTGAATGCGGCGAATGGTgg                                            >  2:1353748/1‑149 (MQ=255)
                                                                                                       aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATgcggc                                                     >  1:689365/1‑140 (MQ=255)
                                                                                                       aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCg                                           <  2:267028/150‑1 (MQ=255)
                                                                                                       aCTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCg                                           <  1:1512586/150‑1 (MQ=255)
                                                                                                        cTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGt                                          >  1:1069640/1‑150 (MQ=255)
                                                                                                          tGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGa                                        <  1:1330148/150‑1 (MQ=255)
                                                                                                           gCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGAc                                       >  2:1528211/1‑150 (MQ=255)
                                                                                                            ccTTATCTTCGTTCTCCGAACAAAAAAATTCAAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGTTGGAATGAATGCGGCGAATGGTGCGTGACt                                      >  1:1609750/1‑150 (MQ=255)
                                                                                                                aTCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGcc                                  >  2:455523/1‑150 (MQ=255)
                                                                                                                 tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGGATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcg                                 >  2:846195/1‑150 (MQ=255)
                                                                                                                 tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcg                                 >  2:70590/1‑150 (MQ=255)
                                                                                                                 tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcg                                 <  1:1053618/150‑1 (MQ=255)
                                                                                                                 tCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCAGCGAATGGTGCGTGACTTGCcg                                 <  2:927133/150‑1 (MQ=255)
                                                                                                                  cTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                >  1:348919/1‑150 (MQ=255)
                                                                                                                  cTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGGGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCcgc                                >  2:314419/1‑150 (MQ=255)
                                                                                                                      gTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGAt                            >  1:1375849/1‑150 (MQ=255)
                                                                                                                      gTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGAGTGACTTGCCGCGGAt                            >  2:101556/1‑150 (MQ=255)
                                                                                                                       ttCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATgcgg                                                      >  1:373381/1‑123 (MQ=255)
                                                                                                                        tctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATca                          >  2:1277023/1‑150 (MQ=255)
                                                                                                                         ctcCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcag                         >  2:145704/1‑150 (MQ=255)
                                                                                                                           ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagca                       <  1:1277023/150‑1 (MQ=255)
                                                                                                                           ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagca                       <  1:395605/150‑1 (MQ=255)
                                                                                                                           ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagca                       <  1:318790/150‑1 (MQ=255)
                                                                                                                           ccGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagca                       <  2:891516/150‑1 (MQ=255)
                                                                                                                            cGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATcagcag                      <  2:1224393/150‑1 (MQ=255)
                                                                                                                             gAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGCATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCagcagc                     >  2:508711/1‑150 (MQ=255)
                                                                                                                             caaCAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAAACAAACAGAGGATGTGCACCTGCATTTACGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCagcagc                     >  1:123601/2‑150 (MQ=255)
                                                                                                                                cAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGtt                  >  1:867493/1‑150 (MQ=255)
                                                                                                                                cAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGtt                  >  2:1162501/1‑150 (MQ=255)
                                                                                                                                    aaaaTTCCAGTCCCGAATGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATTGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCgg              <  2:1239379/150‑1 (MQ=255)
                                                                                                                                    aaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCgg              <  1:1571680/150‑1 (MQ=255)
                                                                                                                                    aaaaTTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCgg              >  2:1607585/1‑150 (MQ=255)
                                                                                                                                      ccttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTa            >  1:1451221/3‑150 (MQ=255)
                                                                                                                                      ccttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTa            >  2:1595470/3‑150 (MQ=255)
                                                                                                                                       cttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg           >  1:851539/2‑150 (MQ=255)
                                                                                                                                       cttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg           >  2:45109/2‑150 (MQ=255)
                                                                                                                                       cttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg           >  1:966175/2‑150 (MQ=255)
                                                                                                                                       cttCCAGTCCTTCGGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGAATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg           >  2:1080267/2‑150 (MQ=255)
                                                                                                                                       aTTACAGTACCGAAGGACTGGAAGGCTCAATCGATCAAATCATTCAGAGTATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATTGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAg           <  2:1115873/150‑1 (MQ=255)
                                                                                                                                          ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTcg        >  1:1549528/1‑150 (MQ=255)
                                                                                                                                          ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGAcg        >  1:1212985/1‑150 (MQ=255)
                                                                                                                                          ccAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATAAGAGGATGTGCACCTGCATTTCCGGGTATTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCAGCGGATCAGCAGCGTTTCGGTAGTcg        >  1:948327/1‑150 (MQ=255)
                                                                                                                                               cccGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGAGata   >  1:110897/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGGGGCGTGAACGGTGAGACTAAACAGGGCGTCGGTCCAGTTCTGCACTTTAGTGACTTTGCCTGTTACCCAATCAGCCATGTTTTTCTCCTGTTTTGATTGACTTGCCTTATCTTCGTTCTCCGAACAAAAAAATTCCAGTCCCGAAGGACTGGAAGGCTCAATCGATCAAATCAATCAGAGGATGTGCACCTGCATTTCCGGGTCTTTGCGATCCAGATAGTGGATGGACTGAATGCGGCGAATGGTGCGTGACTTGCCGCGGATCAGCAGCGTTTCGGTAGTCGCGATAT  >  NC_000913/4114391‑4114684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: