Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,118,828 T→G 7.4% intergenic (+68/+17) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,118,8280TG 7.4% 455.1 / 20.4 158intergenic (+68/+17)zapB/rraAFtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
Reads supporting (aligned to +/- strand):  ref base T (65/81);  new base G (4/8);  total (69/89)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 159 total aligned reads displayed.

GAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGG  >  NC_000913/4118680‑4118975
                                                                                                                                                    |                                                                                                                                                   
gagaACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAAATTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCtt                                                                                                                                                    >  2:530497/1‑150 (MQ=255)
 agaACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTg                                                                                                                                                   >  1:311091/1‑150 (MQ=255)
  gaACAACCATCTGAAAGAACATCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGTAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCATAAAAAAGGCACCTTGc                                                                                                                                                  <  1:394743/150‑1 (MQ=255)
    acaacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCgg                                                                                                                                                >  1:693226/1‑150 (MQ=255)
     caacCAAATGAAAGAACAGCAGAACGGCAGCCAGGAACGTCTGCAGGCCCTCCAGGGACGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAAATTCTTAAATCTGTAGTCCAACAGTTAACGAAGAAAAAAGGCACCTTGCGGt                                                                                                                                               >  2:1049360/1‑150 (MQ=255)
     aaacCATCTGAAAGAACAGCAGAACGGCTGTCAGGAACGTCTGCAGGCCCTTCTGGGTCGCATGGACGAGGTCTGATTACGCTTCAGCTTCCGCGTAATTTCTTTCCTGAGTAGGCCAACAGCTAACGCAGAAAAAAGGCACCTTGCGGt                                                                                                                                               <  1:270052/149‑1 (MQ=255)
      aacCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACTTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTg                                                                                                                                              <  2:719133/150‑1 (MQ=255)
       acCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCTTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                             <  1:350269/150‑1 (MQ=255)
       acCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCTTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGc                                                                                                                                             <  1:965014/150‑1 (MQ=255)
        ccATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                                                                                                                            <  1:1084464/150‑1 (MQ=255)
        ccATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                                                                                                                            <  2:1037175/150‑1 (MQ=255)
          aTCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCtt                                                                                                                                          <  1:686545/150‑1 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                                                                                                                       >  2:432598/1‑150 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                                                                                                                       >  2:1076141/1‑150 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCCTAATTTCATTACTCTGAAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGccatact                                                                                                                                       >  2:292860/1‑145 (MQ=255)
             tGAAAGAACAGCAGAACGGCTGGCACGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCttttt                                                                                                                                       <  1:1100570/150‑3 (MQ=255)
              gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                                                                                                      >  1:47123/1‑150 (MQ=255)
              gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                                                                                                      <  1:530497/150‑1 (MQ=255)
              gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                                                                                                      >  1:1698466/1‑150 (MQ=255)
              gAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                                                                                                                      <  2:888811/150‑1 (MQ=255)
                aaGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAt                                                                                                                                    >  1:1580093/1‑150 (MQ=255)
                 aGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttctg                                                                                                                                   <  2:20795/150‑4 (MQ=255)
                 aGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACACTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATc                                                                                                                                   <  2:467220/150‑1 (MQ=255)
                  gAACAGCATAACGGCTGGCAGTAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                                                                                                  <  2:115915/150‑1 (MQ=255)
                  gAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                                                                                                                  >  2:839157/1‑150 (MQ=255)
                     cagcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTc                                                                                                                               >  2:551537/1‑150 (MQ=255)
                      agcagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCa                                                                                                                              <  2:1249318/150‑1 (MQ=255)
                        cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                                                                                                                            <  2:1253193/150‑1 (MQ=255)
                         agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                                                                                                                           <  2:311091/150‑1 (MQ=255)
                          gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAatat                                                                                                                          >  2:1668753/1‑150 (MQ=255)
                            aCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                                                                                                                        <  2:1108039/150‑1 (MQ=255)
                            aCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                                                                                                                        <  2:923801/150‑1 (MQ=255)
                                cTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                                                                                                                    <  1:161933/150‑1 (MQ=255)
                                cTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                                                                                                                    <  2:450804/150‑1 (MQ=255)
                                 tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGGTTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                                                                                                                   <  1:1600984/150‑1 (MQ=255)
                                 tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                                                                                                                   <  1:1040135/150‑1 (MQ=255)
                                   gCAGGAACGTCTACAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                                                                                                                 >  1:702066/1‑150 (MQ=255)
                                    cAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                                                                                                                <  2:876482/150‑1 (MQ=255)
                                      ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTATGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttc                                                                                                                                     >  2:1511063/1‑126 (MQ=255)
                                      ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                                                                                                              >  2:369808/1‑150 (MQ=255)
                                      ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCGCAAGGTGCCttttttc                                                                                                                                     <  1:1511066/127‑2 (MQ=255)
                                             cTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCGTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg                                                                                                       >  2:154547/1‑150 (MQ=255)
                                               gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGACACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa                                                                                                     >  2:1441801/1‑150 (MQ=255)
                                                cAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaat                                                                                                    >  1:118691/1‑150 (MQ=255)
                                                 aGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata                                                                                                   <  1:587823/150‑1 (MQ=255)
                                                  ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                  >  2:75240/1‑150 (MQ=255)
                                                  ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa                                                                                                  <  2:801004/150‑1 (MQ=255)
                                                   gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat                                                                                                 >  1:1239155/1‑150 (MQ=255)
                                                   gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat                                                                                                 >  1:787146/1‑150 (MQ=255)
                                                       ggctgGGTCGCATGGAATAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGTATCTTCTGAAAGAATAATCCCg                                                                                             <  2:231206/149‑1 (MQ=255)
                                                        gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCgg                                                                                            >  1:413166/1‑150 (MQ=255)
                                                            ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAtt                                                                                        >  1:1293879/1‑150 (MQ=255)
                                                            ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAtt                                                                                        >  1:1380257/1‑150 (MQ=255)
                                                               cGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTc                                                                                     >  2:1194178/1‑150 (MQ=255)
                                                               cGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTc                                                                                     >  2:1187900/1‑150 (MQ=255)
                                                                 cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCgg                                                                                   <  1:468788/150‑1 (MQ=255)
                                                                 cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCgg                                                                                   <  1:1027899/150‑1 (MQ=255)
                                                                   tGGTAGAGGTATGATTACGCTTCATCTTCCGCTTTATTTCTTTACTTTGTAGGCCAACAGTTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAGCATTCAATATCCAGCGTATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                                 <  1:292860/150‑1 (MQ=255)
                                                                   tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCa                                                                                 <  1:432598/150‑1 (MQ=255)
                                                                     gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATa                                                                               >  1:724738/1‑150 (MQ=255)
                                                                     gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATa                                                                               >  2:1665976/1‑150 (MQ=255)
                                                                       agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATaaa                                                                             <  2:705543/150‑1 (MQ=255)
                                                                              tGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTc                                                                      >  2:1290155/1‑150 (MQ=255)
                                                                                  tACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCg                                                                  <  1:1589540/150‑1 (MQ=255)
                                                                                   aCGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCgg                                                                 <  1:974722/150‑1 (MQ=255)
                                                                                     gCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCATAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATTGTCGCCGgag                                                               <  2:1142004/150‑1 (MQ=255)
                                                                                           tCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGaagaag                                                         >  2:1714701/1‑150 (MQ=255)
                                                                                            cTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAgg                                                        <  1:839157/150‑1 (MQ=255)
                                                                                              tCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTg                                                      >  2:1558227/1‑150 (MQ=255)
                                                                                                 gcgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGTTGaca                                                   <  2:489935/150‑1 (MQ=255)
                                                                                                  cgTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGacac                                                  >  1:1132990/1‑150 (MQ=255)
                                                                                                     aaTTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGc                                               >  1:619877/1‑150 (MQ=255)
                                                                                                        ttCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCaa                                            >  1:1581454/1‑150 (MQ=255)
                                                                                                          cTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCaaaa                                          <  1:347308/150‑1 (MQ=255)
                                                                                                          cTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGACATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCaaaa                                          >  1:275483/1‑150 (MQ=255)
                                                                                                           tttACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAt                                         >  1:1625461/1‑150 (MQ=255)
                                                                                                                     aGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGAc                               <  1:196034/150‑1 (MQ=255)
                                                                                                                        ccAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATc                            <  2:1625461/150‑1 (MQ=255)
                                                                                                                         cAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCg                           <  2:164180/150‑1 (MQ=255)
                                                                                                                          aaCAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGc                          >  1:1586967/1‑150 (MQ=255)
                                                                                                                            cAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCtt                        <  1:1076141/150‑1 (MQ=255)
                                                                                                                             aGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCttt                       <  1:781879/150‑1 (MQ=255)
                                                                                                                              ggTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                      <  1:229047/150‑1 (MQ=255)
                                                                                                                              ggTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                      <  2:1380257/150‑1 (MQ=255)
                                                                                                                              ggTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                      >  2:578762/1‑150 (MQ=255)
                                                                                                                              ggTAACGCAGAAAAAAGACACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTc                      <  1:1441801/150‑1 (MQ=255)
                                                                                                                               gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                     >  2:1290807/1‑150 (MQ=255)
                                                                                                                               gTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                     <  1:154547/150‑1 (MQ=255)
                                                                                                                                tAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGc                    <  2:1609061/150‑1 (MQ=255)
                                                                                                                                 aaCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGTATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                   <  1:986819/150‑1 (MQ=255)
                                                                                                                                 aaCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                   <  2:118691/150‑1 (MQ=255)
                                                                                                                                 aaCGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGcc                   <  1:60559/150‑1 (MQ=255)
                                                                                                                                   cGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCaa                 >  2:1589217/1‑150 (MQ=255)
                                                                                                                                     cAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgcg                                  >  1:111845/1‑131 (MQ=255)
                                                                                                                                     cAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGAcgcg                                  <  2:111845/131‑1 (MQ=255)
                                                                                                                                      ataagaaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGc              >  1:1427338/6‑150 (MQ=255)
                                                                                                                                           aaaGGCACCGCAAGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCg         >  1:1652476/1‑150 (MQ=255)
                                                                                                                                             aGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGcc       >  1:765539/1‑150 (MQ=255)
                                                                                                                                               gCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAg     >  2:253470/1‑150 (MQ=255)
                                                                                                                                                  ccTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCgg  >  1:1272344/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGG  >  NC_000913/4118680‑4118975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: