Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,121,627 T→A 7.4% N41Y (AAC→TAC) ‡ hslU ← molecular chaperone and ATPase component of HslUV protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,121,6270TA 7.4% 347.7 / 13.9 130N41Y (AAC→TAC) ‡hslUmolecular chaperone and ATPase component of HslUV protease
Reads supporting (aligned to +/- strand):  ref base T (59/60);  new base A (6/4);  total (65/65)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 133 total aligned reads displayed.

GCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTAAT  >  NC_000913/4121478‑4121775
                                                                                                                                                     |                                                                                                                                                    
gCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                                                                                                                      >  1:688474/1‑150 (MQ=255)
gCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                                                                                                                      >  2:408590/1‑150 (MQ=255)
 cccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                     <  2:456360/150‑1 (MQ=255)
 cccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                     >  1:1581725/1‑150 (MQ=255)
 cccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGGCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                     <  2:59858/150‑1 (MQ=255)
  ccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGa                                                                                                                                                    <  2:436117/150‑1 (MQ=255)
     ctgcGGTGAATTTGGGCGCGGCTCCTTTGATCCACGGCGCCTTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGTTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGTTCTTCGTTGAGCt                                                                                                                                                 <  1:67996/147‑1 (MQ=255)
      ttCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTg                                                                                                                                                <  1:102399/150‑1 (MQ=255)
         ggTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCAt                                                                                                                                             >  1:1376565/1‑150 (MQ=255)
          gTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCAACGAAGAGCTGcgcc                                                                                                                                            <  1:512754/150‑4 (MQ=255)
           tGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCGTGGCGCAGCTCTTCGTTGAGCTGCATGc                                                                                                                                           >  2:323954/1‑150 (MQ=255)
                 tGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGcc                                                                                                                                     <  2:113002/150‑1 (MQ=255)
                   gTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAg                                                                                                                                   <  1:1172948/150‑1 (MQ=255)
                       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCATACGACTGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTAACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCCGCGGt                                                                                                                               >  1:1026089/1‑150 (MQ=255)
                       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGt                                                                                                                               >  1:324913/1‑150 (MQ=255)
                       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGt                                                                                                                               >  1:219790/1‑150 (MQ=255)
                       cTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGt                                                                                                                               <  1:1335233/150‑1 (MQ=255)
                          cAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGCCACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTAACATCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTAc                                                                                                                            >  2:251539/1‑150 (MQ=255)
                          cAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTAc                                                                                                                            <  1:342537/150‑1 (MQ=255)
                           aaCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACg                                                                                                                           <  2:1581725/150‑1 (MQ=255)
                           aaCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGAATTCAGTTTTACCGACACCGGTCGGGCCGATCATCCGGATATTTTTCGGGGTCTCTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGGTACg                                                                                                                           >  2:253812/1‑150 (MQ=255)
                            aCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                                                                                                          >  1:787747/1‑150 (MQ=255)
                               ttGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCCGCGCTTAGTTGAGCTGCATGCGACGCCAGCGGTTACGCaga                                                                                                                       >  1:1033572/1‑150 (MQ=255)
                               ttGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCaga                                                                                                                       >  2:290205/1‑150 (MQ=255)
                                tgatgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                                                                                                                      >  2:737983/1‑150 (MQ=255)
                                 gctgaACGGCGCAGGCGCCAGCGTAGCCAGACGCCGGGCGATTTCAGTTTGACCGACACCGGTCGGGCCGAGCATCAGGATATTTATCGGGGGCACTTCATGGCGCAGCTCTGCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGc                                                                                                                     <  1:882608/148‑1 (MQ=255)
                                     aagggCGCATTCGGTAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGAAATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATc                                                                                                                 <  2:594412/147‑1 (MQ=255)
                                      aCGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                                                                                                                <  2:688474/150‑1 (MQ=255)
                                      aCGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                                                                                                                <  1:494102/150‑1 (MQ=255)
                                       cGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGc                                                                                                               <  1:657326/150‑1 (MQ=255)
                                         gcgcATTCGCCAGCTTAGCCAGACGACGGGGGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGAAATTATTCGGGGTCCCTTCATGGCGCCGCTCTTCGGTGAGCTGCATGCGACGCCAGCTGTAACGCAGAGCAATCGccc                                                                                                             >  1:332049/1‑149 (MQ=255)
                                          cgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcac                                                                                                            >  2:715932/1‑150 (MQ=255)
                                               tCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAAc                                                                                                       >  1:1635721/1‑150 (MQ=255)
                                                cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACg                                                                                                      >  2:1327753/1‑150 (MQ=255)
                                                  ccAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATAATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCt                                                                                                    >  1:1507402/1‑150 (MQ=255)
                                                        tAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGt                                                                                              >  2:1145621/1‑150 (MQ=255)
                                                        tAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGt                                                                                              >  2:1085248/1‑150 (MQ=255)
                                                          gCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg                                                                                            >  2:7030/1‑150 (MQ=255)
                                                            cAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTc                                                                                          <  1:290205/150‑1 (MQ=255)
                                                            cAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTc                                                                                          <  1:375556/150‑1 (MQ=255)
                                                             aGACGACGGGCGATTTCAGTGTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGTGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTcc                                                                                         <  2:671673/150‑1 (MQ=255)
                                                               acgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGCCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTg                                                                                       <  1:301679/150‑1 (MQ=255)
                                                                cgacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATACTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTgg                                                                                      >  2:1635907/1‑150 (MQ=255)
                                                                  ccgGGCGATTTCAGTTTTACCGACACCGGGCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGcc                                                                                    <  1:1493418/149‑1 (MQ=255)
                                                                  acgGGCGATTTCAGTTTTACCGACTCCGGTAGGGCCGAGCATCAGGATATTTTTCGGGGTCACTTCATGGCGCTGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGcc                                                                                    <  1:288602/150‑1 (MQ=255)
                                                                   cgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCg                                                                                   >  2:621802/1‑150 (MQ=255)
                                                                   cgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCg                                                                                   <  1:7704/150‑1 (MQ=255)
                                                                     ggCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgat                                                                                 >  2:417075/1‑150 (MQ=255)
                                                                     ggCGATTTCAGGTTTACCGACACCGGTCGGGCCAATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgat                                                                                 <  2:273901/150‑1 (MQ=255)
                                                                      gCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgatg                                                                                >  2:988344/1‑150 (MQ=255)
                                                                      gCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGGTGTCCTGGCCgatg                                                                                >  1:1286679/1‑150 (MQ=255)
                                                                          tttCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGAtgt                                                                            >  2:859653/1‑150 (MQ=255)
                                                                               tttttACCGAGACCGGTCGGGCCGATCATCAGTATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTa                                                                       <  2:1378911/149‑1 (MQ=255)
                                                                                  ttACCGACACCGGTCGGGTCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATcc                                                                    <  1:1386158/150‑1 (MQ=255)
                                                                                     ccGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGt                                                                 <  1:996339/150‑1 (MQ=255)
                                                                                          acCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCt                                                            <  2:1633477/150‑1 (MQ=255)
                                                                                             ggTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGAc                                                         <  1:641558/150‑1 (MQ=255)
                                                                                              gTCGGGCCGATCATCATGATATTTTTCTGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACg                                                        <  2:1171672/150‑1 (MQ=255)
                                                                                              gTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACg                                                        <  2:1600570/150‑1 (MQ=255)
                                                                                                 gggCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGAtt                                                     >  1:1128727/1‑150 (MQ=255)
                                                                                                   gCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGGCGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGCTGTGCTTATCCAGTTCGCTGAAGAttta                                                   >  1:1055579/1‑149 (MQ=255)
                                                                                                    ccGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcg                                                  >  2:771204/1‑150 (MQ=255)
                                                                                                    ccGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcg                                                  <  1:540590/150‑1 (MQ=255)
                                                                                                    ccGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcg                                                  >  1:860045/1‑150 (MQ=255)
                                                                                                    ccGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCGTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcg                                                  >  2:132327/1‑150 (MQ=255)
                                                                                                     cGATCATCAGGATATTTTTCGGGGTCACTTCATGGTGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcgc                                                 >  2:1043123/1‑150 (MQ=255)
                                                                                                       atcatcAGGATATTTTTCGGGGCCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGACCGCTTGGCGGTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGt                                               <  1:1502589/150‑1 (MQ=255)
                                                                                                         catcaGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTgg                                             <  2:736114/150‑1 (MQ=255)
                                                                                                              ggATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCa                                        >  2:1293835/1‑150 (MQ=255)
                                                                                                              ggATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTGAGCCAGTTCGCTGCCGATGTCGCGTGGGGTCa                                        >  2:1047190/1‑150 (MQ=255)
                                                                                                                 tatTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCAttt                                     >  1:1483557/1‑150 (MQ=255)
                                                                                                                  atTTTTCGGGGTCTCTTCATGGCGCATGTCTTCGTTGAGTTGCATGCGACGCGAGCGGTTACGCAGAGCAATCGCCACAGAACTCTTGGAGTTGTCCTGGCCTATTATTTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTc                                    <  2:992346/150‑1 (MQ=255)
                                                                                                                   tttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCa                                   <  1:432082/150‑1 (MQ=255)
                                                                                                                    ttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGCACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAg                                  >  2:827535/1‑150 (MQ=255)
                                                                                                                    ttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAg                                  >  1:444676/1‑150 (MQ=255)
                                                                                                                        cGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGGTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGCCGATTTCGCGTGGGGTCATTTCAGACAt                              >  1:868912/1‑150 (MQ=255)
                                                                                                                          gggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATgg                            >  2:1698112/1‑150 (MQ=255)
                                                                                                                          gggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATgg                            <  2:324913/150‑1 (MQ=255)
                                                                                                                           ggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATggg                           <  1:1364159/150‑1 (MQ=255)
                                                                                                                           ggTCACTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATggg                           <  1:716515/150‑1 (MQ=255)
                                                                                                                            gTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGga                          >  2:1359348/1‑150 (MQ=255)
                                                                                                                             gcACTTCCTGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCATTCGCCACAGAACGCTTGGCGTTGTCTTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGgag                         <  1:253812/149‑1 (MQ=255)
                                                                                                                                cTTCGTGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATc                      <  1:323954/150‑1 (MQ=255)
                                                                                                                                cTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGTCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATc                      <  1:1008266/150‑1 (MQ=255)
                                                                                                                                 ttCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATcc                     <  2:423239/150‑1 (MQ=255)
                                                                                                                                 ttCATGGCGCAGCTCTTCGTGGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATcc                     <  2:1507402/150‑1 (MQ=255)
                                                                                                                                  tCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCt                    >  2:1384266/1‑150 (MQ=255)
                                                                                                                                   cATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCtt                   >  1:415003/1‑150 (MQ=255)
                                                                                                                                   cATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCtt                   >  1:742353/1‑150 (MQ=255)
                                                                                                                                      catgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTTGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACg                >  2:962768/4‑150 (MQ=255)
                                                                                                                                      catgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACg                >  2:509196/4‑150 (MQ=255)
                                                                                                                                      catgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACg                >  1:406477/4‑150 (MQ=255)
                                                                                                                                      catgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACg                >  1:1679981/4‑150 (MQ=255)
                                                                                                                                       gcgcAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGc               <  1:1151136/150‑1 (MQ=255)
                                                                                                                                         gcAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCtt             <  1:621802/150‑1 (MQ=255)
                                                                                                                                         gcAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCtt             <  1:1406749/150‑1 (MQ=255)
                                                                                                                                             ctctTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTa         <  1:1327753/150‑1 (MQ=255)
                                                                                                                                                  cGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTa    <  2:1373213/150‑1 (MQ=255)
                                                                                                                                                   gTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTaa   <  1:1293835/150‑1 (MQ=255)
                                                                                                                                                    ttGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTAAt  <  2:1152816/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTAAT  >  NC_000913/4121478‑4121775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: