Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,127,251 C→T 6.7% intergenic (+26/+35) rpmE → / ← yiiX 50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,127,2510CT 6.7% 320.7 / 5.1 102intergenic (+26/+35)rpmE/yiiX50S ribosomal subunit protein L31/putative lipid binding hydrolase, DUF830 family protein
Reads supporting (aligned to +/- strand):  ref base C (44/51);  new base T (3/4);  total (47/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTC  >  NC_000913/4127102‑4127399
                                                                                                                                                      |                                                                                                                                                    
 gACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCa                                                                                                                                                     <  2:1618091/150‑1 (MQ=255)
  aCCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCAc                                                                                                                                                    >  2:199629/1‑150 (MQ=255)
    cTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACgg                                                                                                                                                  <  2:1454957/150‑1 (MQ=255)
     ggAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgc                                                                                                                                                 <  2:1512775/149‑1 (MQ=255)
      gAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgcg                                                                                                                                                <  1:1578793/150‑1 (MQ=255)
      gAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgcg                                                                                                                                                <  1:449962/150‑1 (MQ=255)
      gAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgcg                                                                                                                                                <  2:33548/150‑1 (MQ=255)
      gAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgcg                                                                                                                                                <  1:107878/150‑1 (MQ=255)
         ccTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCtt                                                                                                                                             >  1:1632129/1‑150 (MQ=255)
         ccTCGACGTGTGCAGCAAGTGCCACCCGTTCATCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTACAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGgcgcat                                                                                                                                             >  2:1036579/1‑148 (MQ=255)
          cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttt                                                                                                                                            <  1:1686924/150‑1 (MQ=255)
          cTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCttt                                                                                                                                            <  1:904765/150‑1 (MQ=255)
               cGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGTGCGGCGCttttttcg                                                                                                                                       <  1:1346930/150‑3 (MQ=255)
               cGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTtg                                                                                                                                       >  2:1266727/1‑150 (MQ=255)
                gtgtGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGGTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAAGCGCCGTGCGGCGCttttttcg                                                                                                                                       <  1:1079539/150‑3 (MQ=255)
                gtgtGCAGCAAGGGCCACCCGTGCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCAAAAAAAGCGCCGTGCGGCGCttttttcgg                                                                                                                                      <  1:1211105/150‑4 (MQ=255)
                   tgCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGcc                                                                                                                                   >  1:1387443/1‑150 (MQ=255)
                    gcagcaAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGGGGCCGGGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGAATTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCt                                                                                                                                  <  1:103885/150‑1 (MQ=255)
                     cagcaAGTGCCACCCGTTCTTCACTGGCACACAGCGTGATGTTGCTCCCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAAAAAGACCGGATTTCCGAAAAAAGCGCCTCACGGCGCTTTTTTTGTGCCtc                                                                                                                                 >  2:1002242/1‑149 (MQ=255)
                        caAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAt                                                                                                                              >  1:636457/1‑150 (MQ=255)
                          aGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAttt                                                                                                                            >  2:471817/1‑150 (MQ=255)
                           gTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAtttt                                                                                                                           <  2:365006/150‑1 (MQ=255)
                             gCCACCCGTTCTTCACTGGCATACAGCGTGATGTTGCTACCGGTGGACGTGTTGACCGCTTCAACAAGCGTTTCATCATCCCGGGCAGCAAATAAGACCGGATTTCCGATAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTgg                                                                                                                         <  1:277828/150‑1 (MQ=255)
                                   cGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCGTTTATTGTGCCTGAATTTTGGTCGTAt                                                                                                                   >  1:956529/1‑150 (MQ=255)
                                     ttcttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTa                                                                                                                 <  1:508228/150‑1 (MQ=255)
                                       cttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTAcc                                                                                                               <  1:186484/150‑1 (MQ=255)
                                        ttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              <  2:636457/150‑1 (MQ=255)
                                        ttcACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCa                                                                                                              >  1:1102728/1‑150 (MQ=255)
                                            cTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGCCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaa                                                                                                          >  2:620587/1‑150 (MQ=255)
                                              ggCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGaaaa                                                                                                        >  2:794373/1‑150 (MQ=255)
                                               gCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAAc                                                                                                       <  2:599947/150‑1 (MQ=255)
                                                cAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAAAAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATATCCTAAAAAAGCGCCCCACGGCGCTTTTTTAGTGACAGAATTTTGGTCGTATTACCACGaaaaaa                                                                                                      >  1:589007/1‑148 (MQ=255)
                                                    cAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCgg                                                                                                  <  1:994621/150‑1 (MQ=255)
                                                     aGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGc                                                                                                 <  2:520844/150‑1 (MQ=255)
                                                      gCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGcc                                                                                                >  2:665327/1‑150 (MQ=255)
                                                              ttGCTACCGGGGGCCGTGTTGACCGCTTCAACTAGCGATTCAACATCCCGGGCAGCAAATAAGACCGGAGTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTg                                                                                        <  2:1004426/150‑1 (MQ=255)
                                                               tGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTgg                                                                                       <  2:37133/150‑1 (MQ=255)
                                                               tGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTgg                                                                                       <  1:1288232/150‑1 (MQ=255)
                                                               tGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTgg                                                                                       >  2:1140541/1‑150 (MQ=255)
                                                                gCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGc                                                                                      <  1:1140541/150‑1 (MQ=255)
                                                                 cTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  1:1603537/1‑150 (MQ=255)
                                                                 cTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCg                                                                                     >  1:622363/1‑150 (MQ=255)
                                                                    ccGGTGGCCGTGTTGACCGCTTCTACAAGCGTTTCAACATCCCGGGCAGCAAATAAGTCCGGATTTCCGAAAATAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGAcc                                                                                  <  2:1669236/150‑1 (MQ=255)
                                                                        tGGCCGTTTTGACCGCTTCAACAAGCGTTTCATCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGTCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtgg                                                                              <  1:1011646/150‑1 (MQ=255)
                                                                        tGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtgg                                                                              <  1:199629/150‑1 (MQ=255)
                                                                          gCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtggtg                                                                            <  1:1422998/150‑1 (MQ=255)
                                                                          gCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCgtggtg                                                                            <  2:1239749/150‑1 (MQ=255)
                                                                          gCCGTGTTGACCGCTTCAACAAGCGTTGCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCatggtg                                                                            <  2:6520/150‑1 (MQ=255)
                                                                             gtgtTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGt                                                                         <  1:1036579/150‑1 (MQ=255)
                                                                             gtgtTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGt                                                                         >  2:262005/1‑150 (MQ=255)
                                                                                 tGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCAGTCTTTGGCGACCGTGGTGAGTtgtg                                                                     >  1:1193276/1‑150 (MQ=255)
                                                                                  gACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTgg                                                                    >  2:1545773/1‑150 (MQ=255)
                                                                                  gACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTgg                                                                    >  2:175392/1‑150 (MQ=255)
                                                                                   aCCGCTTCAACAAGCGTTTCATCATGCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgc                                                                   <  2:853182/150‑1 (MQ=255)
                                                                                    ccGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGgcg                                                                  <  2:225486/150‑1 (MQ=255)
                                                                                      gCTGCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCa                                                                <  2:1210923/150‑1 (MQ=255)
                                                                                       cTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCaa                                                               >  1:480617/1‑149 (MQ=255)
                                                                                        ttCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATc                                                              <  2:768524/150‑1 (MQ=255)
                                                                                         tCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCg                                                             <  2:773292/150‑1 (MQ=255)
                                                                                         tCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTACGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTTCCACGAAAACATCGTCCATTCTTTGGCGACCTTGGTGAGTGGTCGCGAATCg                                                             >  2:324843/1‑150 (MQ=255)
                                                                                            acaaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGACGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaaa                                                          >  1:574266/1‑150 (MQ=255)
                                                                                             caaGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGaaaa                                                         >  1:732724/1‑150 (MQ=255)
                                                                                               aGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAAGTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACg                                                       >  1:89494/1‑150 (MQ=255)
                                                                                                 cGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGc                                                     >  1:939273/1‑150 (MQ=255)
                                                                                                   tttCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGGATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCtt                                                   >  2:151343/1‑150 (MQ=255)
                                                                                                       aaCATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCTCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTgg                                               <  1:1546395/150‑1 (MQ=255)
                                                                                                         cATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCg                                             >  1:946725/1‑150 (MQ=255)
                                                                                                          aTCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCga                                            <  1:638003/150‑1 (MQ=255)
                                                                                                             ccGGTCAGCAAATAAGACCTGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGac                                         <  1:400951/150‑1 (MQ=255)
                                                                                                             ccGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGac                                         >  1:1476653/1‑150 (MQ=255)
                                                                                                                   agcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTTGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGt                                   <  2:1168721/150‑1 (MQ=255)
                                                                                                                      aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTc                                <  1:175392/150‑1 (MQ=255)
                                                                                                                       aaTAAGACCTGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGCCCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTcc                               <  2:249943/150‑1 (MQ=255)
                                                                                                                       aaTAAGAACGGATTTCCGAAAAAAGCGCCGCGCGGGGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGCCAACAGCGGCGACTGTTTGGCGCCCGTGGGGCGGAGTGGCGCATCGAAACCGCCTTGTGGCGAGACGCCCGTTTcc                               >  1:1067434/1‑150 (MQ=255)
                                                                                                                        aTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTGTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAACACGGCTTGTGGCGAGACCCCCGTTTccg                              >  1:1511496/1‑149 (MQ=255)
                                                                                                                         tAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTc                             >  1:1540784/1‑150 (MQ=255)
                                                                                                                            gACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGGCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAg                          <  1:858648/150‑1 (MQ=255)
                                                                                                                            gACAGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAg                          >  2:1117195/1‑150 (MQ=255)
                                                                                                                             aCCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc                         >  2:664717/1‑150 (MQ=255)
                                                                                                                             aCCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGc                         >  2:492578/1‑150 (MQ=255)
                                                                                                                              ccGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCg                        <  2:797301/150‑1 (MQ=255)
                                                                                                                               cGGATTTCCGAAAACAGCGCCGCACGGCGCTTTTTTTGTGCCTTAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCgg                       <  1:1027830/150‑1 (MQ=255)
                                                                                                                                ggATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGa                      <  2:1387443/150‑1 (MQ=255)
                                                                                                                                ggATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGa                      >  1:1660742/1‑150 (MQ=255)
                                                                                                                                  aTTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAat                    >  1:476106/1‑150 (MQ=255)
                                                                                                                                       cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCt               <  2:326142/150‑1 (MQ=255)
                                                                                                                                       cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCt               >  1:1356134/1‑150 (MQ=255)
                                                                                                                                       cGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCt               >  1:1610668/1‑150 (MQ=255)
                                                                                                                                       acaaaaaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTc                >  1:1164593/4‑150 (MQ=255)
                                                                                                                                          aaaaaaGCGCCGTGCGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTg             >  2:1626102/1‑150 (MQ=255)
                                                                                                                                           aaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGc            <  1:404911/150‑1 (MQ=255)
                                                                                                                                           aaaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGc            >  1:1045223/1‑150 (MQ=255)
                                                                                                                                            aaaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGcc           >  1:770497/1‑150 (MQ=255)
                                                                                                                                             aaaGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCg          <  1:1545773/150‑1 (MQ=255)
                                                                                                                                               aGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTa        >  1:595827/1‑150 (MQ=255)
                                                                                                                                                gcgcCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACAGTTTCCTCAAGCGGAATATTCTTGCCGTaa       <  1:471817/150‑1 (MQ=255)
                                                                                                                                                 cgccgcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc      <  1:1700649/150‑1 (MQ=255)
                                                                                                                                                 cgccgcACGGAGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGAGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAAc      <  2:1603537/150‑1 (MQ=255)
                                                                                                                                                  gccgcACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACg     >  2:795485/1‑150 (MQ=255)
                                                                                                                                                     gcACGGCGCTTTTTTTGAGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCAATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGTCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTc  <  1:261243/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TGACCTGAACCTCGACGTGTGCAGCAAGTGCCACCCGTTCTTCACTGGCAAACAGCGTGATGTTGCTACCGGTGGCCGTGTTGACCGCTTCAACAAGCGTTTCAACATCCCGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTTCCTCAAGCGGAATATTCTTGCCGTAACGTTC  >  NC_000913/4127102‑4127399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: