Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,132,434 T→A 6.6% intergenic (+167/‑182) metL → / → metF Bifunctional aspartokinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,132,4340TA 6.6% 544.8 / 13.3 185intergenic (+167/‑182)metL/metFBifunctional aspartokinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (100/72);  new base A (4/9);  total (104/81)
Fisher's exact test for biased strand distribution p-value = 8.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

TGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAA  >  NC_000913/4132285‑4132583
                                                                                                                                                     |                                                                                                                                                     
 gCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATa                                                                                                                                                      >  1:965407/1‑150 (MQ=255)
  cccGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATAc                                                                                                                                                     >  1:657305/1‑150 (MQ=255)
  cccGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATAc                                                                                                                                                     >  1:1261107/1‑150 (MQ=255)
   ccGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATAcc                                                                                                                                                    >  1:444727/1‑150 (MQ=255)
   ccGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATAcc                                                                                                                                                    <  2:819215/150‑1 (MQ=255)
     ggTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCgg                                                                                                                                                  <  2:690656/150‑1 (MQ=255)
     ggTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCgg                                                                                                                                                  <  2:936939/150‑1 (MQ=255)
      gTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGa                                                                                                                                                 <  2:741269/150‑1 (MQ=255)
      gTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGa                                                                                                                                                 <  1:1504498/150‑1 (MQ=255)
      gTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGa                                                                                                                                                 <  2:704991/150‑1 (MQ=255)
      gTACTCATGTTTTCGGGGTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGa                                                                                                                                                 <  2:1357396/150‑1 (MQ=255)
       tACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTATGCAAAGCAAACCGGac                                                                                                                                                <  2:333114/150‑2 (MQ=255)
          tCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAtttt                                                                                                                                             >  1:1580982/1‑150 (MQ=255)
           cATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttc                                                                                                                                            >  2:834502/1‑150 (MQ=255)
           cATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttc                                                                                                                                            >  1:1082348/1‑150 (MQ=255)
             tGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGAAGTCCGGTTTGATTTGCATACCGGAttttctt                                                                                                                                          >  1:1222941/1‑150 (MQ=255)
                tttCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGAttttcttttt                                                                                                                                       <  2:1052127/150‑1 (MQ=255)
                   cGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATtttctttttctt                                                                                                                                    >  1:1393626/1‑150 (MQ=255)
                     ggTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTAc                                                                                                                                  >  2:1670995/1‑150 (MQ=255)
                         tATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGGTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTAACAta                                                                                                                              >  2:1512541/1‑149 (MQ=255)
                             gTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGa                                                                                                                          >  2:46023/1‑150 (MQ=255)
                             gTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGa                                                                                                                          >  2:257304/1‑150 (MQ=255)
                             gTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGGTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGa                                                                                                                          <  1:715790/150‑1 (MQ=255)
                                 cTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGtt                                                                                                                      >  2:1479018/1‑150 (MQ=255)
                                   aaTGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGtttt                                                                                                                    <  1:1208967/150‑1 (MQ=255)
                                      gAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTc                                                                                                                 >  2:119721/1‑150 (MQ=255)
                                          tatatTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCt                                                                                                             >  2:54137/1‑150 (MQ=255)
                                             atTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTcc                                                                                                          >  1:821639/1‑150 (MQ=255)
                                                gAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTTGTTTATATTTGCAGTCCGGTTTGATTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTg                                                                                                       >  1:82978/1‑150 (MQ=255)
                                                   ttATCCTATGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGACCATGGGTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGAtt                                                                                                    <  2:1113338/150‑1 (MQ=255)
                                                       cATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTc                                                                                                >  2:913300/1‑150 (MQ=255)
                                                       cATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTc                                                                                                >  1:1369992/1‑150 (MQ=255)
                                                           ggATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCa                                                                                            <  1:466887/150‑1 (MQ=255)
                                                           ggATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCa                                                                                            >  2:717261/1‑150 (MQ=255)
                                                            gATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCAc                                                                                           <  2:881555/150‑1 (MQ=255)
                                                            gATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCAc                                                                                           >  2:1665677/1‑150 (MQ=255)
                                                                 ggCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACcatca                                                                                      >  2:1312684/1‑150 (MQ=255)
                                                                  gCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACcatcat                                                                                     >  2:1000653/1‑150 (MQ=255)
                                                                    cGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTg                                                                                   <  2:475202/150‑1 (MQ=255)
                                                                     ggATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTgg                                                                                  >  1:1036037/1‑150 (MQ=255)
                                                                          aaGAGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCAtt                                                                             <  2:472485/150‑1 (MQ=255)
                                                                             cGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCAttttt                                                                          >  1:1593682/1‑150 (MQ=255)
                                                                               tttACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCg                                                                        <  2:529470/150‑1 (MQ=255)
                                                                                ttACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTctg                                                                       >  1:778749/1‑148 (MQ=255)
                                                                                  acgacgAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGtt                                                                     >  2:216876/1‑150 (MQ=255)
                                                                                   cgacgaATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTg                                                                    >  1:746658/1‑150 (MQ=255)
                                                                                      cgaATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGGTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACg                                                                 <  1:257304/150‑1 (MQ=255)
                                                                                       gaATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGc                                                                >  2:1269441/1‑150 (MQ=255)
                                                                                            ctgCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTTCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGAATTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                           <  2:560180/148‑1 (MQ=255)
                                                                                            cGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCtta                                                           >  1:477009/1‑149 (MQ=255)
                                                                                             ggCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                           <  1:1528976/149‑1 (MQ=255)
                                                                                             ggCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTc                                                           >  2:1528976/1‑149 (MQ=255)
                                                                                             ggCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCg                                                          <  2:1213101/150‑1 (MQ=255)
                                                                                             ggCAAGAAGCAATAACTACATGGTTAGTTTATATTTGCAGTCAGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCg                                                          <  1:945269/150‑1 (MQ=255)
                                                                                              gCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCgg                                                         <  2:1448079/150‑1 (MQ=255)
                                                                                              gCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCgg                                                         >  2:787254/1‑150 (MQ=255)
                                                                                               cAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGc                                                        <  1:1479018/150‑1 (MQ=255)
                                                                                               cAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCCGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGc                                                        <  1:906348/150‑1 (MQ=255)
                                                                                                aagaagCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCt                                                       <  1:216876/150‑1 (MQ=255)
                                                                                                     gCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTcc                                                  >  2:381302/1‑150 (MQ=255)
                                                                                                     gCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTcc                                                  >  1:863500/1‑150 (MQ=255)
                                                                                                      cAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACAATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCt                                                 >  1:1254026/1‑150 (MQ=255)
                                                                                                      cAATAAGTACATGGTTAGTTTATATATGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACAATCCTGAAGTTTTTTCATCTTCCCCGATTTTTACTCACCATCATTGGTCATTTTTCGGTTTACTCCATTCGGCTTTTCCt                                                 >  1:245765/1‑150 (MQ=255)
                                                                                                       aaTAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCCTTTTTCGGTTGACGCCCTTCGGCTTTTCCtt                                                <  1:1135261/150‑1 (MQ=255)
                                                                                                        aTAAGTACATGGGTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTc                                               <  2:1603985/150‑1 (MQ=255)
                                                                                                           aGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATc                                            <  1:1003950/150‑1 (MQ=255)
                                                                                                             tACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCtt                                          >  2:1213028/1‑150 (MQ=255)
                                                                                                              aCATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCttt                                         >  1:1696177/1‑150 (MQ=255)
                                                                                                               cATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTa                                        <  2:1580982/150‑1 (MQ=255)
                                                                                                                 tGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACa                                      >  2:338726/1‑150 (MQ=255)
                                                                                                                    ttAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCt                                   >  2:1413650/1‑150 (MQ=255)
                                                                                                                      aGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTgg                                 >  1:1104757/1‑150 (MQ=255)
                                                                                                                      aGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATATTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTgg                                 >  1:583208/1‑150 (MQ=255)
                                                                                                                        tttATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGAc                               <  2:412294/150‑1 (MQ=255)
                                                                                                                        tttATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACg                              <  1:717261/150‑1 (MQ=255)
                                                                                                                         ttATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACg                              <  1:913300/150‑1 (MQ=255)
                                                                                                                              tttGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCGTTTCCTTCATCTTTACATCTGGACGTCTaa                         >  2:343597/1‑150 (MQ=255)
                                                                                                                              aagaaaatCCGGTATGCAAAGCAAACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTATGTTGACGCCCTTCGGCTTTTCCTTAATCTTTAAATCTGGACGTCTaa                         >  1:1709310/8‑150 (MQ=255)
                                                                                                                              aagaaaatCCGGTATGCAAAGCAAACCGGATTTTCTTTTTCTTACCATACTGAAGTTTTTTCATCTTCCCTGATTTTTGCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCGTTTCCTTCATCTTTACATCTGGACGTCTaa                         >  1:1248880/8‑150 (MQ=255)
                                                                                                                                tGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAAc                       <  1:389978/150‑1 (MQ=255)
                                                                                                                                 gCAGTCCGGATTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACg                      <  1:838819/150‑1 (MQ=255)
                                                                                                                                    atCCGGTATGCAAAGCAAACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGAt                   >  1:504055/2‑150 (MQ=255)
                                                                                                                                     tctggTTTGCTTTGCATAACGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATa                  <  1:989005/147‑1 (MQ=255)
                                                                                                                                     tCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATa                  <  1:1269441/150‑1 (MQ=255)
                                                                                                                                     tCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATa                  <  2:863500/150‑1 (MQ=255)
                                                                                                                                      ccGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCACTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAg                 >  1:38782/1‑150 (MQ=255)
                                                                                                                                        ggTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGAt               <  1:419255/150‑1 (MQ=255)
                                                                                                                                          tttgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGAtgt             <  2:746658/150‑1 (MQ=255)
                                                                                                                                            tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGc           <  1:1664235/150‑1 (MQ=255)
                                                                                                                                            tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGc           >  2:279057/1‑150 (MQ=255)
                                                                                                                                            tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGc           >  2:155037/1‑150 (MQ=255)
                                                                                                                                            tgctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGc           >  2:1256535/1‑150 (MQ=255)
                                                                                                                                              ctttgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcac         >  2:388867/1‑150 (MQ=255)
                                                                                                                                                 tgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaac      >  2:943318/1‑150 (MQ=255)
                                                                                                                                                 tgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaac      >  2:486511/1‑150 (MQ=255)
                                                                                                                                                 tgcATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaac      <  1:381723/150‑1 (MQ=255)
                                                                                                                                                   cATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaacac    <  1:31517/150‑1 (MQ=255)
                                                                                                                                                    aTACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaacaca   >  1:1657606/1‑150 (MQ=255)
                                                                                                                                                     tACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaacacaa  >  2:28342/1‑150 (MQ=255)
                                                                                                                                                     tACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCAACATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGcacaacacaa  >  1:757488/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTTGCTTTGCATACCGGATTTTCTTTTTCTTACCATCCTGAAGTTTTTTCATCTTCCCTGATTTTTCCTCACCATCATTGGTCATTTTTCGGTTGACGCCCTTCGGCTTTTCCTTCATCTTTACATCTGGACGTCTAAACGGATAGATGTGCACAACACAA  >  NC_000913/4132285‑4132583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: