Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,136,048 T→C 5.4% intergenic (+33/‑60) katG → / → yijE catalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,136,0480TC 5.4% 373.4 / 9.2 129intergenic (+33/‑60)katG/yijEcatalase‑peroxidase HPI, heme b‑containing/EamA‑like transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (63/58);  new base C (4/3);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

Only 100 of 130 total aligned reads displayed.

GTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGA  >  NC_000913/4135899‑4136195
                                                                                                                                                     |                                                                                                                                                   
  ccTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCg                                                                                                                                                   <  1:1231573/150‑1 (MQ=255)
   cTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGc                                                                                                                                                  >  1:99643/1‑150 (MQ=255)
     gcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACTAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTg                                                                                                                                                <  2:636391/150‑1 (MQ=255)
     gcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTg                                                                                                                                                <  2:1149772/150‑1 (MQ=255)
     gcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTg                                                                                                                                                <  1:668400/150‑1 (MQ=255)
     gcgtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGACTGCCAGCAAGCAGCCGCTg                                                                                                                                                <  1:1119555/150‑1 (MQ=255)
       gtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGaa                                                                                                                                              >  1:721166/1‑150 (MQ=255)
       gtgcgGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGaa                                                                                                                                              <  1:1039646/150‑1 (MQ=255)
             tGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTAGTTAAAGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCt                                                                                                                                        >  2:37542/1‑150 (MQ=255)
                   aaGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCa                                                                                                                                  <  1:1163712/150‑1 (MQ=255)
                    aGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGGCGCTGAACGTTCTTTACCAg                                                                                                                                 <  1:1104732/150‑1 (MQ=255)
                      tttACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCg                                                                                                                               <  2:940013/150‑1 (MQ=255)
                        tACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGta                                                                                                                             >  1:1068069/1‑150 (MQ=255)
                          cGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGtata                                                                                                                           >  1:1191900/1‑150 (MQ=255)
                            ccAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGGGGCGGCATGGGTGAAAGTGATGAACCGCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGt                                                                                                                         <  2:796319/150‑1 (MQ=255)
                                tAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGc                                                                                                                     >  2:1531155/1‑150 (MQ=255)
                                     aTGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacg                                                                                                                <  1:1231164/150‑1 (MQ=255)
                                       gCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGcgaacgaa                                                                                                              >  1:1489753/1‑150 (MQ=255)
                                         ccACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCAACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGaaaa                                                                                                            >  2:1705131/1‑150 (MQ=255)
                                          cACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAAc                                                                                                           >  2:966529/1‑150 (MQ=255)
                                           aCGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACt                                                                                                          >  1:163681/1‑150 (MQ=255)
                                            cGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTa                                                                                                         <  2:555504/150‑1 (MQ=255)
                                             gagaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTac                                                                                                        <  1:981569/150‑1 (MQ=255)
                                              agaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTaca                                                                                                       <  1:1584997/150‑1 (MQ=255)
                                               gaAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGGTCTTTACCAGCTTATAGTGGGCGAACGAAAACTacac                                                                                                      >  2:306255/1‑150 (MQ=255)
                                                 aGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTacacac                                                                                                    >  1:685889/1‑150 (MQ=255)
                                                  gTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACt                                                                                                   >  2:282867/1‑150 (MQ=255)
                                                    ttGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGAGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCCAACGAAAACTACACACTgc                                                                                                 >  1:722637/1‑149 (MQ=255)
                                                     tGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGa                                                                                                <  2:721166/150‑1 (MQ=255)
                                                      gTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTTCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAt                                                                                               >  1:709452/1‑150 (MQ=255)
                                                       ttAACGACTTCGTGGCGGCATGGGTGAAAGTGAAGAACCTCGACCGTTTCGACCTGCTGTACTCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCTCTGAACTTTCTTTACCATCGTATAGTGGTCGAACGAAAACTACACACTGGAtc                                                                                              <  2:571680/150‑1 (MQ=255)
                                                         aaaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctc                                                                                            >  2:1644580/1‑150 (MQ=255)
                                                          aaGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGAtctct                                                                                           <  2:112542/150‑1 (MQ=255)
                                                            gACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                         >  2:28552/1‑150 (MQ=255)
                                                            gACTTCGTGGCGGCATGGGTGAAAGTGATGAACATCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCa                                                                                         >  2:1234154/1‑150 (MQ=255)
                                                             aCTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCAt                                                                                        >  1:999809/1‑150 (MQ=255)
                                                             aCTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCAt                                                                                        >  1:551885/1‑150 (MQ=255)
                                                              cTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGGTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATg                                                                                       <  2:221470/150‑1 (MQ=255)
                                                               ttCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGt                                                                                      <  1:921282/150‑1 (MQ=255)
                                                                    ggcggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGcc                                                                                 <  1:1204042/150‑1 (MQ=255)
                                                                    ggcggcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGcc                                                                                 >  1:1670454/1‑150 (MQ=255)
                                                                       ggcTTGGGTGAAAGTGATGACCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGGTGGCAGTCGGTGAACGTTCTTTACCAGCGTATAGTGGTCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCa                                                                              <  2:1605281/150‑1 (MQ=255)
                                                                        gcATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAg                                                                             <  1:890405/150‑1 (MQ=255)
                                                                          aTGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGa                                                                           <  2:1392341/150‑1 (MQ=255)
                                                                          aTGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGa                                                                           <  2:1435673/150‑1 (MQ=255)
                                                                          aTGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGa                                                                           <  1:1448132/150‑1 (MQ=255)
                                                                           tGGGTGAAAGTGATGCACCTCGCCCGTTTCGACCTGCTGTATTCTGCCCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACGGGATCTCTCATGTCTGCCGCCGGaa                                                                          >  2:684684/1‑150 (MQ=255)
                                                                           tGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCATCTGCTGCTTGCTGGCAGTCGCTGAACGTTATTTACCAGCGTAAAGTGGGCGAACGAAAACAACACACTCGATCTCTCATGTCAGCCGCAGGaa                                                                          >  2:607802/1‑150 (MQ=255)
                                                                            gggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGaaa                                                                         >  2:50785/1‑150 (MQ=255)
                                                                             ggTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACTCTGGATCTCTCATGTCTGCCGCAGGAAag                                                                        >  1:333992/1‑150 (MQ=255)
                                                                               tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                                      <  1:1028469/150‑1 (MQ=255)
                                                                               tGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAagag                                                                      <  1:1331531/150‑1 (MQ=255)
                                                                                gAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGc                                                                     <  2:163681/150‑1 (MQ=255)
                                                                                  aaGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAATAGCaa                                                                   >  1:1318151/1‑150 (MQ=255)
                                                                                    gTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAAcc                                                                 <  1:12373/150‑1 (MQ=255)
                                                                                     tgatgaACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGGATAGTGGGCGAACGAAAACTACACACTTGATCTCTCATGTCTGACGCAGGAAAGAGCCAccc                                                                >  2:203697/1‑150 (MQ=255)
                                                                                         gaACCTCGACCGTTTCGACCTTCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTCTAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTg                                                            <  1:250932/150‑1 (MQ=255)
                                                                                           aCCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGc                                                          >  1:666178/1‑150 (MQ=255)
                                                                                            ccTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCa                                                         >  2:899797/1‑150 (MQ=255)
                                                                                            ccTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCa                                                         >  1:638289/1‑150 (MQ=255)
                                                                                            ccTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCa                                                         >  1:868547/1‑150 (MQ=255)
                                                                                               cGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGCACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCGGCCGCAGGAAAGAGCAACCCACGGGCCATc                                                      >  1:1445185/1‑150 (MQ=255)
                                                                                               cGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATc                                                      <  1:966529/150‑1 (MQ=255)
                                                                                               cGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATc                                                      >  1:407020/1‑150 (MQ=255)
                                                                                                        cGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTg                                             >  2:1478451/1‑150 (MQ=255)
                                                                                                        cGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTg                                             >  1:1379528/1‑150 (MQ=255)
                                                                                                         gACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTgg                                            <  1:1005457/150‑1 (MQ=255)
                                                                                                             tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATTGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgt                                        >  2:1670694/1‑150 (MQ=255)
                                                                                                             tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATGGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgt                                        >  2:673550/1‑150 (MQ=255)
                                                                                                             tgctgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTtgt                                        <  2:868547/150‑1 (MQ=255)
                                                                                                                tgTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCt                                     <  1:8578/150‑1 (MQ=255)
                                                                                                                       tGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTAGCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCTTGTCTGCCGCGGGAAAGAGCAACCCACTGGCAATCCGTGGCCTGGTTGTGCTCACACtt                              >  1:296049/1‑150 (MQ=255)
                                                                                                                        gACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTGACACttt                             >  1:509655/1‑149 (MQ=255)
                                                                                                                          ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATc                           >  2:676969/1‑150 (MQ=255)
                                                                                                                          ccccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATc                           <  1:455368/150‑1 (MQ=255)
                                                                                                                           cccGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCt                          >  2:1404381/1‑150 (MQ=255)
                                                                                                                            acGTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTg                         >  2:453953/2‑150 (MQ=255)
                                                                                                                              gTTCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGa                       >  2:1668101/1‑150 (MQ=255)
                                                                                                                                tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt                     >  1:449845/1‑150 (MQ=255)
                                                                                                                                tCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt                     >  1:908547/1‑150 (MQ=255)
                                                                                                                                tCAGCGACTGCCAGCAAGCAGCCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGt                     >  2:1356230/1‑150 (MQ=255)
                                                                                                                                 cAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGtt                    <  1:1629718/150‑1 (MQ=255)
                                                                                                                                 cAGCGGCTGCTTGCTGGCAGTCGCTGAACGGTCAGTACCAGCGTATGGTGGGCGGGCGAAAACTAGCCACTGGCTCTCTCATGTCTGCCGCAGGAAAGACCAACCCACTGGCAAACAGTGGCCTGGTTGTGCTCACACTTATCTGGAggt                    >  1:253362/1‑148 (MQ=255)
                                                                                                                                  aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTTGTTGTGCTCACACTTATCTGGAGTta                   >  1:1114707/1‑150 (MQ=255)
                                                                                                                                  aGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTta                   >  1:43023/1‑150 (MQ=255)
                                                                                                                                   gCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTtat                  >  1:535794/1‑150 (MQ=255)
                                                                                                                                        tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAATACTACACACTGGATCTCTCATGTCTGCCGCAGGTAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg             <  1:953666/150‑1 (MQ=255)
                                                                                                                                        tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGTTCTCTCATGTCTGCCGCAGGAAAGAGCAACCCCCTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg             <  2:722637/150‑1 (MQ=255)
                                                                                                                                        tgcttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTg             >  2:791779/1‑150 (MQ=255)
                                                                                                                                         gctttctGGCAGTCGCTGAAGGTTGTTTACAAGTGTTTAGTGGTCGAACGAAATCTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTgg            <  2:1318151/150‑1 (MQ=255)
                                                                                                                                          cttgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGa           <  1:278079/150‑1 (MQ=255)
                                                                                                                                            tgctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTgg            <  2:599901/147‑1 (MQ=255)
                                                                                                                                              ctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTAGACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGAtttt       >  2:888508/1‑150 (MQ=255)
                                                                                                                                              ctGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGCCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGAtttt       >  2:503731/1‑150 (MQ=255)
                                                                                                                                              ctGGCAGTCGCTGAACGTTATTTACCAGCGTATAGTGGGCGAACGAAAACTACACGCTGGCTCTCGCTTGTCTGCCGCAGGAAAGCGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATATGGAGTTATAGATGGAtttt       >  2:97546/1‑150 (MQ=255)
                                                                                                                                                ggCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCa     >  1:1144566/1‑150 (MQ=255)
                                                                                                                                                ggCAGTCGCTGAACGTTATTTACCAGCGTATAGGGTGCGAAAGAAAACTACACACTGGATCTCTCATTTATGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTAACACTTATCTGGAGTTATAGCTGGAttttga     >  2:1184273/1‑148 (MQ=255)
                                                                                                                                                 gCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCATGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCAt    <  2:1261901/150‑1 (MQ=255)
                                                                                                                                                 gCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCAt    <  2:443984/150‑1 (MQ=255)
                                                                                                                                                   aGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAATACTACACACTGGATCTCTCATGTCTGCCGCACGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGa  <  1:34430/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTCCTGCGTGCGGTGGCGGAAGTTTACGCCAGTAGCGATGCCCACGAGAAGTTTGTTAAAGACTTCGTGGCGGCATGGGTGAAAGTGATGAACCTCGACCGTTTCGACCTGCTGTAATCTGACCCCGTTCAGCGGCTGCTTGCTGGCAGTCGCTGAACGTTCTTTACCAGCGTATAGTGGGCGAACGAAAACTACACACTGGATCTCTCATGTCTGCCGCAGGAAAGAGCAACCCACTGGCAATCAGTGGCCTGGTTGTGCTCACACTTATCTGGAGTTATAGCTGGATTTTCATGA  >  NC_000913/4135899‑4136195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: