Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,168,350 A→T 8.7% intergenic (+150/‑22) rrsB → / → gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,168,3500AT 8.7% 196.1 / 6.6 77intergenic (+150/‑22)rrsB/gltT16S ribosomal RNA of rrnB operon/tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base A (34/36);  new base T (4/3);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

CCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCT  >  NC_000913/4168201‑4168499
                                                                                                                                                     |                                                                                                                                                     
ccttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCaa                                                                                                                                                       >  1:645679/1‑150 (MQ=39)
ccttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCaa                                                                                                                                                       >  2:807280/1‑150 (MQ=39)
 cttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAc                                                                                                                                                      >  1:1125939/1‑150 (MQ=39)
 cttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCATTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAc                                                                                                                                                      <  2:183275/150‑1 (MQ=255)
  ttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGNAAGTGAAAAGCAAGGCGTTTACGCGTTGNGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcg                                                                                                                                                     >  1:889286/1‑150 (MQ=39)
  ttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcg                                                                                                                                                     <  2:575451/150‑1 (MQ=39)
  ttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcg                                                                                                                                                     <  2:1446361/150‑1 (MQ=39)
   taAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcgc                                                                                                                                                    >  1:44428/1‑150 (MQ=39)
     aagaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGt                                                                                                                                                  >  1:594492/1‑150 (MQ=39)
     aagaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGt                                                                                                                                                  >  2:1241834/1‑150 (MQ=39)
     aagaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGt                                                                                                                                                  >  2:1518095/1‑150 (MQ=39)
       gaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGATAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGa                                                                                                                                                <  2:1087960/150‑1 (MQ=255)
       gaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGa                                                                                                                                                <  1:912808/150‑1 (MQ=39)
       gaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGa                                                                                                                                                <  1:551729/150‑1 (MQ=39)
        aagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGAt                                                                                                                                               >  2:164461/1‑150 (MQ=39)
        aagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGAt                                                                                                                                               >  1:1134168/1‑150 (MQ=39)
         cgCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              <  1:920255/149‑1 (MQ=39)
         agCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCGTTGCGTGATa                                                                                                                                              <  1:1352165/150‑1 (MQ=255)
         agCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              >  1:1613584/1‑150 (MQ=39)
         agCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              <  1:1689991/150‑1 (MQ=39)
         agCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              >  2:168009/1‑150 (MQ=39)
         agCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              <  1:506169/150‑1 (MQ=39)
         agCGTACGTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATa                                                                                                                                              <  2:1291631/150‑1 (MQ=255)
          gCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATaa                                                                                                                                             <  1:695227/150‑1 (MQ=39)
           cGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAg                                                                                                                                            >  1:802127/1‑150 (MQ=39)
           cGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAg                                                                                                                                            >  2:560535/1‑150 (MQ=39)
           cGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAg                                                                                                                                            <  2:802127/150‑1 (MQ=39)
            gTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCGTTGCGTGATattt                                                                                                                                           <  1:653425/150‑4 (MQ=255)
            gTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGc                                                                                                                                           >  1:1115100/1‑150 (MQ=39)
             tACTTTGTAGTGCTCACACAGATAGTCTGATAGAAAGAGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACTCGAAAATATCACGCGTTGCGTGATatttt                                                                                                                                          <  2:124857/150‑5 (MQ=14)
               cTTTGTAGTGATAACACAGATTGTCTGATAGAAAGTGAAAAGAAAGTCGTTTACGAGTTTGGAGTGAGGCTGAAGAGAATAATGCCTTTCGCTTTCTATTAATGAAAGCTCAACCTAAACGAAAATATCACGCAAATCGTGATAAGCAAt                                                                                                                                        >  1:1177196/1‑150 (MQ=14)
                 ttGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAAttt                                                                                                                                      <  2:26897/150‑1 (MQ=39)
                   gTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTc                                                                                                                                    <  1:1530768/150‑1 (MQ=38)
                   gTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCATTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTc                                                                                                                                    >  1:1062235/1‑150 (MQ=38)
                      gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgtg                                                                                                                                 <  2:1099523/150‑1 (MQ=37)
                      gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgtg                                                                                                                                 <  2:828254/150‑1 (MQ=37)
                      gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCgtg                                                                                                                                 <  2:1566706/150‑1 (MQ=37)
                        gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTc                                                                                                                               >  2:1141652/1‑150 (MQ=37)
                         cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTcc                                                                                                                              >  2:1714144/1‑150 (MQ=37)
                         cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTcc                                                                                                                              <  2:31901/150‑1 (MQ=37)
                           cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTcccc                                                                                                                            >  1:643431/1‑150 (MQ=37)
                           cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTcccc                                                                                                                            <  1:631654/150‑1 (MQ=37)
                             cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcg                                                                                                                                                     <  1:375233/123‑1 (MQ=38)
                             cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAAcg                                                                                                                                                     >  2:375233/1‑123 (MQ=38)
                             cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCtt                                                                                                                          >  1:1132676/1‑150 (MQ=37)
                               caGATTGTCTGATAGAAAGTGAAAAGCAAGGTGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCg                                                                                                                        <  2:1483641/150‑1 (MQ=37)
                               caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCg                                                                                                                        <  2:1218002/150‑1 (MQ=37)
                                       cTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAgg                                                                                                                >  1:799552/1‑150 (MQ=37)
                                        tGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGc                                                                                                               >  1:267404/1‑150 (MQ=37)
                                         gATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGcc                                                                                                              <  1:976484/150‑1 (MQ=35)
                                            aGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAg                                                                                                           <  2:1678440/150‑1 (MQ=33)
                                            aGAAAGAGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGATCACCCTACACGAAAATATCACGCAACGCGTGATAACCAATTTTCGTGTCCCCTTCGTCTAGAGGccccg                                                                                                           >  2:1200566/1‑148 (MQ=255)
                                             gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAgg                                                                                                          <  2:1188377/150‑1 (MQ=32)
                                             gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAgg                                                                                                          <  2:575766/150‑1 (MQ=32)
                                               aaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGac                                                                                                        <  2:365793/150‑1 (MQ=32)
                                                aGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGaca                                                                                                       >  1:287836/1‑150 (MQ=32)
                                                 gTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGacac                                                                                                      >  1:1232009/1‑150 (MQ=32)
                                                  tGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACAcc                                                                                                     <  1:59573/150‑1 (MQ=32)
                                                   gacaaGCAAGGCGTTTACGCGCTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATAACTCAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCATTGGTCTAGAGGCCCAGGACACCg                                                                                                    <  2:261414/147‑1 (MQ=11)
                                                   gAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCg                                                                                                    >  1:388853/1‑150 (MQ=32)
                                                   gAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCg                                                                                                    >  2:213472/1‑150 (MQ=32)
                                                    aaaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGc                                                                                                   <  2:972275/150‑1 (MQ=32)
                                                      aaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGccc                                                                                                 <  1:1518095/150‑1 (MQ=11)
                                                                                                                                  tACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCtt                     >  1:1100518/1‑150 (MQ=11)
                                                                                                                                   acacGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGTCGCCACTTGCTGGTTTGTGAGTGAAATTCGCCGACCTTa                    <  2:987437/150‑1 (MQ=9)
                                                                                                                                   acacGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTa                    <  1:1018337/150‑1 (MQ=31)
                                                                                                                                    cacGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa                   >  2:1224961/1‑150 (MQ=32)
                                                                                                                                      cGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata                 <  2:1527562/150‑1 (MQ=32)
                                                                                                                                      cGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata                 >  1:1118163/1‑150 (MQ=32)
                                                                                                                                       gAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAatat                >  2:653108/1‑150 (MQ=32)
                                                                                                                                       gAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAatat                >  1:1025783/1‑150 (MQ=32)
                                                                                                                                         gcttatCACGCGTTGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCCTCGACTTGCTGGTTTGTGCGTGAAAGTCGCCGACCTTAATAtct              >  2:284671/4‑150 (MQ=11)
                                                                                                                                         gcttatCACGCGTTGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCCGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct              >  2:1044896/4‑150 (MQ=11)
                                                                                                                                         gcttatCACGCGTTGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct              >  2:1639531/4‑150 (MQ=255)
                                                                                                                                         gcttatCACGCGTTGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtct              >  2:796439/4‑150 (MQ=255)
                                                                                                                                            tatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaa           <  2:643431/150‑1 (MQ=32)
                                                                                                                                            tatCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaa           >  2:971966/1‑150 (MQ=32)
                                                                                                                                            tatCACGCAACGAGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaa           <  1:394051/150‑1 (MQ=17)
                                                                                                                                                   cAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCAt    >  1:495640/1‑150 (MQ=35)
                                                                                                                                                     aCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCt  >  1:488895/1‑150 (MQ=37)
                                                                                                                                                     |                                                                                                                                                     
CCTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAATATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCT  >  NC_000913/4168201‑4168499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: