Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 430,515 T→C 12.5% intergenic (+39/‑90) secF → / → yajD SecYEG protein translocase auxillary subunit/HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913430,5150TC12.5% 215.1 / 20.6 88intergenic (+39/‑90)secF/yajDSecYEG protein translocase auxillary subunit/HNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base T (44/33);  new base C (6/5);  total (50/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTA  >  NC_000913/430368‑430664
                                                                                                                                                   |                                                                                                                                                      
cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGCAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCCGCCGTCAATTCTGCCGTAATCAAGTTACCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                                      >  1:95552/1‑150 (MQ=255)
cATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCg                                                                                                                                                      >  2:1280649/1‑150 (MQ=255)
 aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                                     <  1:1548820/150‑1 (MQ=255)
 aTCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCgg                                                                                                                                                     >  2:1702642/1‑150 (MQ=255)
  tCTATGTGGCATCTGCGCTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCggg                                                                                                                                                    <  2:164162/150‑1 (MQ=255)
    tATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAt                                                                                                                                                 >  2:984674/1‑150 (MQ=255)
       gtgGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttt                                                                                                                                              >  1:503699/1‑150 (MQ=255)
         ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttt                                                                                                                                            >  1:593712/1‑150 (MQ=255)
         ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                                            <  2:1648435/150‑3 (MQ=255)
         ggCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttca                                                                                                                                            <  2:1710169/150‑3 (MQ=255)
          gCATGTGCGTTGGCTCTGAACCTGGGAATGAAGCGCGAACACATTTTGCAGCAGAAAGTGGAAAAAGAAGTGGCGGATCAGCCGTCATTTCTGCCGTATTCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                                           <  2:1673434/150‑4 (MQ=255)
          gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                                           <  2:1188811/150‑1 (MQ=255)
          gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaa                                                                                                                                           <  2:657523/150‑4 (MQ=255)
          gCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGCTGATGTTGAAAATCCCGGTCAGAAGATCGGGAttttttt                                                                                                                                           <  1:1596409/150‑1 (MQ=255)
           cATCTTCGTTTGCTCTGATACTGGGTATGAAGCGCTAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTTATGTTGAAAATCCCGGTCAGAAGATCGTGAtttttttt                                                                                                                                          <  1:590666/150‑1 (MQ=255)
           cATCTGCGTTGGCTCTGAAACTGGGTATGATGCGCTAACACATGTTGCAGCAGAACGCGGAAAAAGAAGTGTCGTATCAGCCGTCAACTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGAtttttttt                                                                                                                                          <  1:266173/150‑1 (MQ=255)
           cATCTGCGTTGGCTCTGAAACTGGGTATGAACCGCGAAGAAATGTTGCAGGAGAAACTGGAAAAAGAACTGGCGGATCAGCCGGCAATTCTTCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGATCTTCTGACCGGGAttttcaac                                                                                                                                          <  2:776071/150‑5 (MQ=255)
            aTCTGCGTTGGCTCTGAAACTGTGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTg                                                                                                                                         <  1:1553515/150‑1 (MQ=255)
                         ctGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATCTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGAtt                                                                                                                            >  2:450089/1‑150 (MQ=255)
                           taaaCTGGGTATTAAGCGCGAACACATGTTTCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCAGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                                          <  2:321371/149‑1 (MQ=255)
                           gAAACTGGGTATGAAGCGCGAACACCTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGGCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGc                                                                                                                          <  1:1203356/150‑1 (MQ=255)
                            aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATATTTTTTGATTTATGGAGATTGCg                                                                                                                         >  2:687231/1‑150 (MQ=255)
                            aaaCTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGGTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCg                                                                                                                         <  2:1636866/150‑1 (MQ=255)
                               cTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGaaa                                                                                                                      >  1:1083918/1‑150 (MQ=255)
                                     aTGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATcccc                                                                                                                <  2:577605/150‑1 (MQ=255)
                                      tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGTGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTATTATTGATGTATGGAGATTGCGAAAATCCCCg                                                                                                               >  1:1167675/1‑150 (MQ=255)
                                      tGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTGATGTATGGAGATTGCGAAAATCCCCGc                                                                                                              <  1:1596794/150‑1 (MQ=255)
                                       gAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCACGc                                                                                                              >  1:95204/1‑150 (MQ=255)
                                        aaGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCACCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCa                                                                                                             <  2:1690147/150‑1 (MQ=255)
                                          gcgcGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATc                                                                                                           >  1:835007/1‑150 (MQ=255)
                                              gcacacCTGTGGCCGCAGAAAGGGCCAAAAGAAGGGGCGGAGCAGCCGTCAAGGCTGCCGTAATCAAGTGCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                                       <  1:1333216/148‑1 (MQ=255)
                                               aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTgg                                                                                                       <  2:439891/150‑1 (MQ=255)
                                               aaCACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTggg                                                                                                      >  1:1139812/1‑150 (MQ=255)
                                                acacATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGa                                                                                                     <  1:1175635/150‑1 (MQ=255)
                                                   caTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAAc                                                                                                  <  1:519590/150‑1 (MQ=255)
                                                    aTGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACt                                                                                                 >  2:852320/1‑150 (MQ=255)
                                                      gTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgc                                                                                               <  1:669501/150‑1 (MQ=255)
                                                       ttGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTgcg                                                                                              <  2:593712/150‑1 (MQ=255)
                                                         gcagcaGAAAGTGGAAAAAGAAGGGGCGGATCAGCCATCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGcgcg                                                                                            >  2:188755/1‑150 (MQ=255)
                                                           agcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTa                                                                                          >  1:675321/1‑150 (MQ=255)
                                                            gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTaa                                                                                         >  2:1417053/1‑150 (MQ=255)
                                                            gcagAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTATGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGGCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTTCGAAAATCCCCGCATCTTGTGAAACTGCGAGTaa                                                                                         >  1:528527/1‑150 (MQ=255)
                                                                     ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                                >  1:936780/1‑150 (MQ=255)
                                                                     ggAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCAGTAATCAAGTTCACGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACAtt                                                                                >  1:1253463/1‑150 (MQ=255)
                                                                       aaaaaGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTc                                                                              >  2:508392/1‑150 (MQ=255)
                                                                          aagaagGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATc                                                                           <  1:1702642/150‑1 (MQ=255)
                                                                             aagGGGCGGATCAGCCGTAAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAg                                                                        <  1:322699/150‑1 (MQ=255)
                                                                              agGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAgg                                                                       >  1:427005/1‑150 (MQ=255)
                                                                                 ggCGGATCAGCCGTCAATTCAGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTaa                                                                    >  2:787942/1‑150 (MQ=255)
                                                                                    ggATCAGCCGTCAATTCTGCCGTAATCATGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGTTTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACtt                                                                 <  1:1476920/150‑1 (MQ=255)
                                                                                       tCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTc                                                              <  2:388632/150‑1 (MQ=255)
                                                                                          gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATg                                                           >  1:1579882/1‑150 (MQ=255)
                                                                                          gCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATg                                                           >  2:1524638/1‑150 (MQ=255)
                                                                                           ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                                          >  1:1286392/1‑150 (MQ=255)
                                                                                           ccGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATgg                                                          >  2:654788/1‑150 (MQ=255)
                                                                                               cAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTat                                                      >  1:470401/1‑150 (MQ=255)
                                                                                                aaTTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatc                                                     >  1:531799/1‑150 (MQ=255)
                                                                                                  ttCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                                   <  2:675321/150‑1 (MQ=255)
                                                                                                     tGCCGTAATCTAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTACCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATccc                                                >  2:1373432/1‑150 (MQ=255)
                                                                                                        cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                                   >  2:1254560/1‑144 (MQ=255)
                                                                                                        cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTatcat                                                   <  1:1254560/144‑1 (MQ=255)
                                                                                                        cGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaa                                             >  1:1211704/1‑150 (MQ=255)
                                                                                                           aaTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCaaaaaa                                          >  2:568612/1‑150 (MQ=255)
                                                                                                            ttCAAGTTCCCGTTGATGTTGAAAATCCCGGTCTGAAGATCGTGATTTTTTTTGATGTATGGAGATTGCGAAAATCGCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                         <  1:450089/149‑1 (MQ=255)
                                                                                                            aTCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAAc                                         >  1:1590166/1‑150 (MQ=255)
                                                                                                             tCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACt                                        >  2:1622576/1‑150 (MQ=255)
                                                                                                               aaGTTCCCGTTGATGTTGAAAATCCCTGTAAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAAGCCTACATTTCATCCTGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                                      <  2:322852/150‑1 (MQ=255)
                                                                                                               aaGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCAGCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTAt                                      <  1:1524638/150‑1 (MQ=255)
                                                                                                                aGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATg                                     <  1:984674/150‑1 (MQ=255)
                                                                                                                  ttCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATgcg                                   >  2:514000/1‑150 (MQ=255)
                                                                                                                      cGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGt                               >  2:1691629/1‑150 (MQ=255)
                                                                                                                         tGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAg                            <  2:471635/150‑1 (MQ=255)
                                                                                                                           tcaaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaa                          >  1:1244313/7‑150 (MQ=255)
                                                                                                                            caaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGaaa                         >  1:1171360/6‑150 (MQ=255)
                                                                                                                             gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                        >  2:451728/1‑150 (MQ=255)
                                                                                                                             gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                        >  1:87970/1‑150 (MQ=255)
                                                                                                                             gTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                        >  1:721853/1‑150 (MQ=255)
                                                                                                                             aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                        >  2:1235901/5‑150 (MQ=255)
                                                                                                                             aaaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAg                        >  2:1353396/5‑150 (MQ=255)
                                                                                                                              aaaaaaaTCCCGATCTTCTGACCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGCAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCGATCATCCCAAAAAACTATGCGCGGTTAGAAAGc                       >  1:987442/4‑150 (MQ=255)
                                                                                                                              aaaaaaaTCCCGATCTTCGGACCGGGATTTTTTGTGATGTATGGAGATTGCGAAAATCCCCGCATCGTGGGAAACTGCGCGTAACCCAACATTTCATCCAGGTAACTTTTCATGGCTATCCTCCCAACAAACTATGCGCGGGTAGAAAGc                       >  1:329641/4‑150 (MQ=255)
                                                                                                                                  aaaTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCt                   <  1:852320/150‑1 (MQ=255)
                                                                                                                                    aTCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGTGGTTAGAAAGCGGCTAt                 <  2:95552/150‑1 (MQ=255)
                                                                                                                                      cccGGTCAGAAGATCGGTATTTTTTTTTATGTATGGAGATTGCGAAAATCCCAGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAATAACTATGCGCGGTTAGAAAGCGGCTATcg               <  1:687231/150‑1 (MQ=255)
                                                                                                                                      cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg               >  1:993227/1‑150 (MQ=255)
                                                                                                                                      cccGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg               >  1:751071/1‑150 (MQ=255)
                                                                                                                                             agaagaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTGAGAAAGCGGCTATCGCGaaaaa        >  2:1483652/1‑150 (MQ=255)
                                                                                                                                              gaagaTCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAg       >  1:769508/1‑150 (MQ=255)
                                                                                                                                                   tCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa  <  1:6329/150‑1 (MQ=255)
                                                                                                                                                   tCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTa  <  1:508392/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CATCTATGTGGCATCTGCGTTGGCTCTGAAACTGGGTATGAAGCGCGAACACATGTTGCAGCAGAAAGTGGAAAAAGAAGGGGCGGATCAGCCGTCAATTCTGCCGTAATCAAGTTCCCGTTGATGTTGAAAATCCCGGTCAGAAGATCGGGATTTTTTTTGATGTATGGAGATTGCGAAAATCCCCGCATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTA  >  NC_000913/430368‑430664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: