Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,196,354 G→C 5.2% A152G (GCC→GGC)  rsd ← stationary phase protein, binds sigma 70 RNA polymerase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,196,3540GC 5.2% 324.5 / 6.8 96A152G (GCC→GGC) rsdstationary phase protein, binds sigma 70 RNA polymerase subunit
Reads supporting (aligned to +/- strand):  ref base G (41/50);  new base C (3/2);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 103 total aligned reads displayed.

CAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATA  >  NC_000913/4196205‑4196500
                                                                                                                                                     |                                                                                                                                                  
cAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcggg                                                                                                                                                    >  2:1001502/1‑150 (MQ=255)
 aGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgggc                                                                                                                                                   <  1:1351083/150‑1 (MQ=255)
   gTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAGTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgggcgg                                                                                                                                                 <  1:935492/150‑1 (MQ=255)
   gTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACgcgggcgg                                                                                                                                                 <  1:1507897/150‑1 (MQ=255)
     aTTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGgcggcg                                                                                                                                               <  2:1065817/150‑1 (MQ=255)
      ttAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGt                                                                                                                                              <  1:969541/150‑1 (MQ=255)
           cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGc                                                                                                                                         <  1:1502637/150‑1 (MQ=255)
           cTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAACTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCCGCCCGCGTCaaac                                                                                                                                         <  2:1655794/150‑3 (MQ=255)
            tGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCACCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCa                                                                                                                                        <  1:913149/150‑1 (MQ=255)
            tGATCACGTTCCGCGGATCCCGAATAAACGGGCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGATTGACGCCGCCCGCGTCAAACa                                                                                                                                        <  2:1210541/150‑1 (MQ=255)
              aTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc                                                                                                                                      <  1:359530/150‑1 (MQ=255)
              aTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc                                                                                                                                      <  2:1642382/150‑1 (MQ=255)
              aTCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc                                                                                                                                      <  2:428853/150‑1 (MQ=255)
               tCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACca                                                                                                                                     <  1:405082/150‑1 (MQ=255)
               tCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGGTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACca                                                                                                                                     <  2:1263515/150‑1 (MQ=255)
                cAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag                                                                                                                                    <  1:1097400/150‑1 (MQ=255)
                 aGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagc                                                                                                                                   <  2:1589761/150‑1 (MQ=255)
                  ggatCCGCGGATCCCGAATAATCGGTCTCAGCCAGTTAACGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGACTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCTCcagca                                                                                                                                  <  1:288353/147‑1 (MQ=255)
                   gTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagcag                                                                                                                                 <  2:1183041/150‑1 (MQ=255)
                   gTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagcag                                                                                                                                 <  1:510427/150‑1 (MQ=255)
                       cgcgGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTAAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATc                                                                                                                             <  2:879142/150‑1 (MQ=255)
                        gcgGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCa                                                                                                                            >  1:1188459/1‑150 (MQ=255)
                              cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTAt                                                                                                                      >  2:91716/1‑150 (MQ=255)
                              cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTAt                                                                                                                      >  1:648678/1‑150 (MQ=255)
                               ccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATc                                                                                                                     >  2:515148/1‑150 (MQ=255)
                               ccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATc                                                                                                                     <  1:109676/150‑1 (MQ=255)
                                   aTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTcc                                                                                                                 <  1:1254298/150‑1 (MQ=255)
                                      aaCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACATCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGc                                                                                                              <  2:1182601/150‑1 (MQ=255)
                                      aaCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGc                                                                                                              >  1:1437595/1‑150 (MQ=255)
                                            ctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAg                                                                                                        >  1:832792/1‑150 (MQ=255)
                                                cccAGTTAAGGCACGCCTACAAGAACTTAGCCCCTCAAATGGGGCATTGAATGTAAAGTACGCGTTAACAGCGCAGAAATCAAGCAGGATGGTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcg                                                                                                    <  1:872787/149‑1 (MQ=255)
                                                 ccAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGACCTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcgc                                                                                                   >  2:1074545/1‑150 (MQ=255)
                                                  cAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt                                                                                                  >  1:655526/1‑150 (MQ=255)
                                                    gTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTc                                                                                                >  2:872511/1‑150 (MQ=255)
                                                     ttAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTcc                                                                                               <  1:1103746/150‑1 (MQ=255)
                                                         ggCACTCCTACAAGAAATTAGCCCCTCATAGGGGGATTTGAATGTAAATTACGCGTTAACAGCGGAGAACTCAAGCAGGATGTTTGACGCGGGCGGCTTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAgcg                                                                                           <  1:1248306/150‑1 (MQ=255)
                                                           cACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGTCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACACAGCGCGCTTCCAGCGcc                                                                                         >  1:1684180/1‑150 (MQ=255)
                                                               ccGACAAGAACTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACCGCGCAGACCTCAAGCAGGATGTTGGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGc                                                                                     <  1:132128/150‑1 (MQ=255)
                                                                cGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACACAGCGCGCTTCCAGCGCCTCGcc                                                                                    >  2:142318/1‑150 (MQ=255)
                                                                 gACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCg                                                                                   <  1:1622194/150‑1 (MQ=255)
                                                                  aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGa                                                                                  >  1:934191/1‑150 (MQ=255)
                                                                  aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGa                                                                                  <  1:840074/150‑1 (MQ=255)
                                                                   cAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGAt                                                                                 <  2:1188459/150‑1 (MQ=255)
                                                                   cAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGAt                                                                                 >  2:1364739/1‑150 (MQ=255)
                                                                   cAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGAt                                                                                 <  2:1374275/150‑1 (MQ=255)
                                                                   cAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGAt                                                                                 >  2:1459268/1‑150 (MQ=255)
                                                                    aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATg                                                                                >  1:338505/1‑150 (MQ=255)
                                                                     aGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCCGCTTATCTTCCAGCACAAAGCGCGCTTCCCGCGCCTCGCCgcggt                                                                               >  1:613053/1‑146 (MQ=255)
                                                                      gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCTGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTc                                                                              >  1:1488630/1‑150 (MQ=255)
                                                                      gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTc                                                                              <  2:688281/150‑1 (MQ=255)
                                                                       aaaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCa                                                                             <  1:918689/150‑1 (MQ=255)
                                                                        aaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAACGCGCGCTTCCAGCGCCTCGCCGATGTCAg                                                                            >  2:729082/1‑150 (MQ=255)
                                                                        aaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAg                                                                            >  2:917336/1‑150 (MQ=255)
                                                                        aaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAg                                                                            <  2:783511/150‑1 (MQ=255)
                                                                        aaTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAg                                                                            >  2:82255/1‑150 (MQ=255)
                                                                         aTTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGa                                                                           <  1:470354/150‑1 (MQ=255)
                                                                           tAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATa                                                                         >  2:189770/1‑150 (MQ=255)
                                                                                ccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAACGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAAcc                                                                    >  1:331783/1‑150 (MQ=255)
                                                                                    aaaTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGtt                                                                <  1:800141/150‑1 (MQ=255)
                                                                                      aTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGa                                                              <  1:917336/150‑1 (MQ=255)
                                                                                      aTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGa                                                              <  1:379506/150‑1 (MQ=255)
                                                                                      aTGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGa                                                              <  1:1593366/150‑1 (MQ=255)
                                                                                       tGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGaa                                                             >  1:703934/1‑150 (MQ=255)
                                                                                        ggggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGaaa                                                            >  1:1267443/1‑150 (MQ=255)
                                                                                          ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACt                                                          >  2:1390726/1‑150 (MQ=255)
                                                                                          ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCGCGCCGATGTCAGATAAAACCTGTTGAAACt                                                          <  1:91716/150‑1 (MQ=255)
                                                                                            cATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCa                                                        >  1:6862/1‑150 (MQ=255)
                                                                                             aTTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCaa                                                       <  2:1401109/150‑1 (MQ=255)
                                                                                              ttGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAg                                                      <  1:1482551/150‑1 (MQ=255)
                                                                                               tGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCTGGCGGCGGCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAgg                                                     <  1:268650/150‑1 (MQ=255)
                                                                                                 aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcg                                                                                                    <  2:760734/101‑1 (MQ=255)
                                                                                                 aaTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcg                                                                                                    >  1:760734/1‑101 (MQ=255)
                                                                                                  aTGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTaa                                                  >  2:441813/1‑150 (MQ=255)
                                                                                                     tAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTa                                               >  1:37858/1‑150 (MQ=255)
                                                                                                        aTTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCa                                            >  1:1620158/1‑150 (MQ=255)
                                                                                                             gcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATc                                       <  1:1364739/150‑1 (MQ=255)
                                                                                                             gcgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATc                                       <  1:1459268/150‑1 (MQ=255)
                                                                                                              cgTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCg                                      >  1:1164869/1‑150 (MQ=255)
                                                                                                               gTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATc                                       >  2:374290/1‑148 (MQ=255)
                                                                                                               gTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATc                                       <  1:374290/148‑1 (MQ=255)
                                                                                                                   aCAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGc                                 >  1:1670671/1‑150 (MQ=255)
                                                                                                                   aCAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGc                                 <  1:515148/150‑1 (MQ=255)
                                                                                                                      gcgcAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGt                              >  2:1682218/1‑150 (MQ=255)
                                                                                                                         cAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTc                           >  2:792209/1‑150 (MQ=255)
                                                                                                                                 atgCAGGTTGTTTGACGCGGGCGGCGTCAAGCACCAGAAGACTCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCTCAGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTgg                   <  2:1418814/148‑1 (MQ=255)
                                                                                                                                     aGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATc               <  2:1437595/150‑1 (MQ=255)
                                                                                                                                      tggtgcttGACGCCGCCCGCGTCAAACACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCg              >  1:1642176/7‑150 (MQ=255)
                                                                                                                                      ggctGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCg              <  1:1670059/147‑1 (MQ=255)
                                                                                                                                        gtgcttGACGCCGCCCGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgta            >  1:1259325/5‑150 (MQ=255)
                                                                                                                                          gcttGACGCCGCCCGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGCTGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCgtagt          >  1:340831/3‑150 (MQ=255)
                                                                                                                                             tGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAAt       >  1:1645208/1‑150 (MQ=255)
                                                                                                                                             tGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAAt       <  1:792209/150‑1 (MQ=255)
                                                                                                                                              gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc      >  2:240771/1‑150 (MQ=255)
                                                                                                                                              gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc      >  2:1682095/1‑150 (MQ=255)
                                                                                                                                              gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc      <  1:1239243/150‑1 (MQ=255)
                                                                                                                                              gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc      <  1:1074545/150‑1 (MQ=255)
                                                                                                                                              gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACGCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATc      >  2:67477/1‑150 (MQ=255)
                                                                                                                                                cgcgGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCa    >  1:1031390/1‑150 (MQ=255)
                                                                                                                                                  cgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATa  >  2:534121/1‑150 (MQ=255)
                                                                                                                                                  cgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGAGGTTTCCAGACTGGAATCGTAGTAATCCATa  >  2:1306101/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CAGGTATTAGCCTGATCAGGTTCCGCGGATCCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATA  >  NC_000913/4196205‑4196500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: