Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,214,432 A→T 6.8% E51D (GAA→GAT metA → homoserine O‑transsuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,4320AT 6.8% 371.8 / 10.8 129E51D (GAA→GATmetAhomoserine O‑transsuccinylase
Reads supporting (aligned to +/- strand):  ref base A (63/57);  new base T (5/4);  total (68/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 135 total aligned reads displayed.

CCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTG  >  NC_000913/4214283‑4214580
                                                                                                                                                     |                                                                                                                                                    
ccGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGaa                                                                                                                                                      >  2:96442/1‑150 (MQ=255)
ccGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAAAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGaa                                                                                                                                                      <  1:1401252/150‑1 (MQ=255)
 cGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGaaa                                                                                                                                                     <  1:1043326/150‑1 (MQ=255)
   aTTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAGCTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATGCTTAACCTGATGCCGATGAAGATTGAAACTGaaatt                                                                                                                                                   >  2:1594165/1‑148 (MQ=255)
    ttCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATc                                                                                                                                                  <  2:1662945/150‑1 (MQ=255)
     tCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCGCGTGCGTCTGGGCAGGAAATTCGTCCCCTTAAGGTTCTGATCCTTATCCTGATGCCGAAGAAGATTGAAACTGAAAGTCa                                                                                                                                                 >  1:1320807/1‑150 (MQ=255)
        tgtgCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGtt                                                                                                                                              >  1:1068132/1‑150 (MQ=255)
         gtgCCGGACGCGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGGCTTTGGGCTGCCACCGTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGCGGCCGAAGAAGATTGAAACTGAAAATCAGttt                                                                                                                                             >  1:132298/1‑150 (MQ=255)
           gCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGATTTTCAGTTTca                                                                                                                                           <  1:1164944/150‑2 (MQ=255)
           gCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCt                                                                                                                                           <  2:1055021/150‑1 (MQ=255)
           gCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCt                                                                                                                                           >  2:636655/1‑150 (MQ=255)
            ccGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTg                                                                                                                                          >  2:671024/1‑150 (MQ=255)
             cGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGATTTTCAGTTTcaat                                                                                                                                         <  1:1364610/150‑4 (MQ=255)
               gACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGATTTTCAGTTTcaatct                                                                                                                                       <  2:1425740/150‑6 (MQ=255)
                     caaCCCGCCGTCAATTTCTTGCGAGAAGAAAACGTCATAGTGATCACAACATATAGTGCGTAATGTCATGAAATTCGTCCACTTAAGGATCTGATGCTTAACCTGATGCCGAAGAAGATTGAAACTGCAAATCAGATTCTGAGCAAGCtt                                                                                                                                 >  2:1531615/3‑150 (MQ=255)
                     cTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCtt                                                                                                                                 >  2:1008438/1‑150 (MQ=255)
                     cTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCAGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCtt                                                                                                                                 >  2:214180/1‑150 (MQ=255)
                      tACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTg                                                                                                                                          <  1:1358579/140‑1 (MQ=255)
                      tACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTg                                                                                                                                          >  2:1358579/1‑140 (MQ=255)
                        cccGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCa                                                                                                                              >  1:500529/1‑150 (MQ=255)
                        cccGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCa                                                                                                                              >  1:234247/1‑150 (MQ=255)
                         ccgccgTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCATGATTTCaa                                                                                                                             >  2:723287/1‑150 (MQ=255)
                                 aaTTTCTTGCGTGAAGAAAACGTCTTTGTTATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCt                                                                                                                     <  1:1103726/150‑1 (MQ=255)
                                 aaTTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCt                                                                                                                     >  1:1208547/1‑150 (MQ=255)
                                  aTTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCtt                                                                                                                    <  1:979687/150‑1 (MQ=255)
                                     tCTTGCGTGAAGAAATCGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGc                                                                                                                 <  1:763808/150‑1 (MQ=255)
                                      cTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCa                                                                                                                <  2:137087/150‑1 (MQ=255)
                                      cTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCa                                                                                                                <  1:901472/150‑1 (MQ=255)
                                      cTTGCGTGAAGAAAACGTCTTTGTGATGACAACTACTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCa                                                                                                                <  1:800272/150‑1 (MQ=255)
                                        tGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAgg                                                                                                              <  1:239326/150‑1 (MQ=255)
                                        tGCGTGAAGAAAACGTCTTTCTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAgg                                                                                                              <  2:1687405/150‑1 (MQ=255)
                                        tGCGTGAAGAAAACGGCTTTGTGATGACAACTTCTCGGGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAgg                                                                                                              <  2:1162398/150‑1 (MQ=255)
                                            tGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGGCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGa                                                                                                          <  2:1291334/150‑1 (MQ=255)
                                             gaagaaAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGat                                                                                                         >  1:218550/1‑150 (MQ=255)
                                             gaagaaAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTGCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGat                                                                                                         <  2:103302/150‑1 (MQ=255)
                                              aagaaAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGata                                                                                                        >  1:521033/1‑150 (MQ=255)
                                              aagaaAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGata                                                                                                        >  2:544750/1‑150 (MQ=255)
                                                  aaaCGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCa                                                                                                    <  1:1202305/150‑1 (MQ=255)
                                                    aCGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGc                                                                                                  >  1:1684502/1‑150 (MQ=255)
                                                    aCGTCTTTGTGATGACAACTTCTCGTGCGTCTGGGCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGc                                                                                                  <  1:294476/150‑1 (MQ=255)
                                                      gTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCACGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCATACTCACCTTTGCAGGTCGATATTCAGCTg                                                                                                <  1:96442/150‑1 (MQ=255)
                                                       tCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCCCTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGt                                                                                               >  2:56320/1‑150 (MQ=255)
                                                       tCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGt                                                                                               <  2:1454894/150‑1 (MQ=255)
                                                        cTTTGTGATGACAACTTCTCGTGCGTCTGTTCAGGAAATTCGTAAACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGAGCCTGCTTTCAAACTCAACTTTGCAGGTCGATATTCAGCTGtt                                                                                              >  2:733617/1‑150 (MQ=255)
                                                         tttGTTATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCTTCCACTTAAGGTTCTGATCCTTAACCTGATGTCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCTAACTCACCTTTGCAGGTCGATATTCATCTGTTg                                                                                             <  1:269960/150‑1 (MQ=255)
                                                         tttGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGCTCCTTAACCTGATGCCGAAGAAGAGTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTg                                                                                             <  2:939435/150‑1 (MQ=255)
                                                              gatgaCAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCAt                                                                                        <  1:1617541/150‑1 (MQ=255)
                                                               atgaCAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATc                                                                                       >  1:1001571/1‑150 (MQ=255)
                                                                     aCTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTcc                                                                                 >  2:176133/1‑150 (MQ=255)
                                                                        tctcGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGt                                                                              <  2:500529/150‑1 (MQ=255)
                                                                        tctcGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTGCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGt                                                                              <  2:234247/150‑1 (MQ=255)
                                                                         ctcGTGCGTCTGGTCAGGATATTCGTCCACTTAAGGTTCTGATCATTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTg                                                                             <  1:1567737/150‑1 (MQ=255)
                                                                         ctcGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTg                                                                             <  2:1587491/150‑1 (MQ=255)
                                                                         ctcGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTg                                                                             <  1:314747/150‑1 (MQ=255)
                                                                           cGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGaa                                                                           >  2:481173/1‑150 (MQ=255)
                                                                                  cTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCa                                                                    <  1:1450357/150‑1 (MQ=255)
                                                                                   tGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCaa                                                                   >  1:852040/1‑150 (MQ=255)
                                                                                     gTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCCGCTGTTGCGCATCGATTCCCGTGAATCGCGCAaca                                                                 >  1:1297968/1‑150 (MQ=255)
                                                                                     gTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAaca                                                                 >  2:393984/1‑150 (MQ=255)
                                                                                        aGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGc                                                              >  1:746371/1‑150 (MQ=255)
                                                                                         ggAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGcc                                                             >  2:172623/1‑150 (MQ=255)
                                                                                         ggAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCACGGGAATcgatg                                                                     >  2:1186665/1‑139 (MQ=255)
                                                                                         ggAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCACGGGAATcgatg                                                                     <  1:1186665/142‑4 (MQ=255)
                                                                                            aaTTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAATAAGATTGAAAATGAAAATCTGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCCGGTCGATATTCAGCTGTTGCGCATCTATTCCCGTGAATCGCGCAACACGCCCGc                                                          <  1:1548821/150‑1 (MQ=255)
                                                                                               tCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCCGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCaga                                                       >  2:665865/1‑150 (MQ=255)
                                                                                                 gTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGc                                                     <  1:176133/150‑1 (MQ=255)
                                                                                                 gTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGc                                                     <  2:9538/150‑1 (MQ=255)
                                                                                                     aCTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCt                                                 <  1:636655/150‑1 (MQ=255)
                                                                                                      cTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTg                                                <  2:748955/150‑1 (MQ=255)
                                                                                                        tAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGaa                                              >  1:1690266/1‑150 (MQ=255)
                                                                                                        tAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGaa                                              >  2:586793/1‑150 (MQ=255)
                                                                                                         aaGGTTCTGTTCCTTAAACTGATGCCGAAGAAGTTTGAAACTGAAATTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTTCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGaac                                             <  2:112149/150‑1 (MQ=255)
                                                                                                            gTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGaacaac                                          >  1:1247919/1‑150 (MQ=255)
                                                                                                             ttCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTATGCGCCTGATTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACt                                         >  2:117160/1‑150 (MQ=255)
                                                                                                              tCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCATTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCAGAACAACtt                                        >  1:757009/1‑150 (MQ=255)
                                                                                                               cTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTc                                       >  1:245581/1‑150 (MQ=255)
                                                                                                                tGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCTATTCACGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCt                                      >  1:282300/1‑150 (MQ=255)
                                                                                                                    ccTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTg                                  <  1:1187924/150‑1 (MQ=255)
                                                                                                                    ccTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTg                                  <  2:1208547/150‑1 (MQ=255)
                                                                                                                    ccTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTg                                  <  2:1101164/150‑1 (MQ=255)
                                                                                                                        aaCCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAAc                              <  2:1128895/150‑1 (MQ=255)
                                                                                                                            tGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTg                          >  2:1437438/1‑150 (MQ=255)
                                                                                                                            tGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTg                          >  2:1417058/1‑150 (MQ=255)
                                                                                                                                   gaagaaGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGatat                   >  2:1597911/1‑150 (MQ=255)
                                                                                                                                      gaagaTTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAACCTCACCTTTGCAGGTCGATATTCAGCTGTTGCGAATCGATTCCGGTGAAACGCGCAACACGCCCGAAGAGCAGCTGAACAAGTTCTACTGTAACTTTGAAGAAATTCa                >  2:975032/1‑150 (MQ=255)
                                                                                                                                       ggcgcagAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAg               >  1:960756/7‑150 (MQ=255)
                                                                                                                                        agaTTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAgg              >  2:409508/1‑150 (MQ=255)
                                                                                                                                        agaTTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAgg              >  2:1225877/1‑150 (MQ=255)
                                                                                                                                          gcagAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGAt            >  2:48477/4‑150 (MQ=255)
                                                                                                                                           ttGAAACTAAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATc           >  1:1089208/1‑150 (MQ=255)
                                                                                                                                           cagAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATc           >  2:469017/3‑150 (MQ=255)
                                                                                                                                           cagAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATc           >  2:1691259/3‑150 (MQ=255)
                                                                                                                                            agAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGTATCa          >  2:904881/2‑150 (MQ=255)
                                                                                                                                             gAAACTGATTTTCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAg         <  1:469017/150‑1 (MQ=255)
                                                                                                                                             gAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCTCCTTTGCTGGTCGATATACAGCTGTTGCGCATCGCTACCCGGGAAGCGCGCAACACGCCAGCAGCGCATCGGTACACCATCGACTGAAACCTGCACTATATTCAGGATCaa         >  2:168762/1‑149 (MQ=255)
                                                                                                                                             gAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAg         >  2:1343564/1‑150 (MQ=255)
                                                                                                                                                aCTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAAc      >  1:632670/1‑150 (MQ=255)
                                                                                                                                                 cTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACt     <  1:214180/150‑1 (MQ=255)
                                                                                                                                                 cTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACt     <  1:1008438/150‑1 (MQ=255)
                                                                                                                                                   gAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACttt   >  1:1387800/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTCGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTG  >  NC_000913/4214283‑4214580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: