Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,242,546 G→A 5.1% intergenic (‑292/+80) xylE ← / ← malG D‑xylose transporter/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,5460GA 5.1% 502.4 / 6.8 154intergenic (‑292/+80)xylE/malGD‑xylose transporter/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (70/75);  new base A (4/4);  total (74/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GATAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGT  >  NC_000913/4242397‑4242694
                                                                                                                                                     |                                                                                                                                                    
  tAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTg                                                                                                                                                    <  2:1258552/150‑1 (MQ=255)
   ataaaCAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATTACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTTCAGACTAAAGAAAAAAAGGCGCTCCGTgg                                                                                                                                                   <  2:179411/148‑1 (MQ=255)
     aaaCAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAg                                                                                                                                                 <  2:960423/150‑1 (MQ=255)
        cAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAgcgc                                                                                                                                              <  1:1572617/150‑1 (MQ=255)
          aTACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCg                                                                                                                                            >  1:1270363/1‑150 (MQ=255)
            aCTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGaa                                                                                                                                          >  2:1372962/1‑150 (MQ=255)
            aCTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCACGGAGCGccttt                                                                                                                                          <  2:1518077/150‑4 (MQ=255)
             atATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCACGGAGCGcctttt                                                                                                                                         <  1:178775/149‑5 (MQ=255)
               aTTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCACGGAGCGcctttttt                                                                                                                                       <  2:880941/150‑7 (MQ=255)
                 tGCCTTGACTAAGAGCACGAAATATAATTATAGTAATTGGTAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACa                                                                                                                                     <  1:1246391/150‑1 (MQ=255)
                  gCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAg                                                                                                                                    <  1:833756/150‑1 (MQ=255)
                  gCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATAAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAg                                                                                                                                    <  2:697687/150‑1 (MQ=255)
                   ccGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGt                                                                                                                                   <  2:646099/150‑1 (MQ=255)
                     gTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTca                                                                                                                                 <  2:1098216/150‑1 (MQ=255)
                     gTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTca                                                                                                                                 >  2:1495710/1‑150 (MQ=255)
                       gACTCAGATCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTcaca                                                                                                                               <  2:804721/150‑1 (MQ=255)
                        aCTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACaa                                                                                                                              >  2:170597/1‑150 (MQ=255)
                         cTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGTTCCGTGGAGCGCCGAATAACAGTCACAAg                                                                                                                             >  2:1718112/1‑150 (MQ=255)
                         cTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAg                                                                                                                             >  1:183360/1‑150 (MQ=255)
                           cAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGtt                                                                                                                           <  2:980115/150‑1 (MQ=255)
                             gagCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTgg                                                                                                                         <  1:788303/150‑1 (MQ=255)
                               gCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGa                                                                                                                       <  2:775092/150‑1 (MQ=255)
                                 aCGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATa                                                                                                                     >  1:1077717/1‑150 (MQ=255)
                                  cGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATaa                                                                                                                    >  1:727333/1‑150 (MQ=255)
                                  cGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATaa                                                                                                                    >  1:487701/1‑150 (MQ=255)
                                   gAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAc                                                                                                                   >  2:797781/1‑150 (MQ=255)
                                   gAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAc                                                                                                                   <  2:870729/150‑1 (MQ=255)
                                    aaaTAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATCATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACg                                                                                                                  <  2:114542/150‑1 (MQ=255)
                                    aaaGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACg                                                                                                                  >  2:541330/1‑150 (MQ=255)
                                     aaGAGAATTATCGTAAGTGGGAAAACAAATAAAGTAAAAATAATAAGCTCTATGATGGAATTATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGt                                                                                                                 <  1:976246/150‑1 (MQ=255)
                                          aaTTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGtt                                                                                                            >  1:1170915/1‑150 (MQ=255)
                                            ttATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCACCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGa                                                                                                          <  2:974913/150‑1 (MQ=255)
                                             tATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAg                                                                                                         >  2:717178/1‑150 (MQ=255)
                                               tCGTAAGTGGGAAAACAAATAACGTAAATTTAATAATCTCTATGATGGAAATATTATCCGGCGAACGATTCAGTTTGCAGACGATAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAggg                                                                                                       <  2:555678/150‑1 (MQ=255)
                                               tCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAggg                                                                                                       <  2:1024533/150‑1 (MQ=255)
                                                    aGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAg                                                                                                  <  1:44091/150‑1 (MQ=255)
                                                    aGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAg                                                                                                  >  1:499332/1‑150 (MQ=255)
                                                        ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAATTATTAACCGGCGAACGATTCAGCTTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCCCAAGTTGGGATAACGTAAGTTGCGGGTGCAGCGGc                                                                                              >  2:1189814/1‑150 (MQ=255)
                                                        ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGc                                                                                              >  2:566645/1‑150 (MQ=255)
                                                        ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGc                                                                                              <  2:1219643/150‑1 (MQ=255)
                                                         gAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGACAAACAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCa                                                                                             <  1:257516/150‑1 (MQ=255)
                                                           aaaCAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCTAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGATGGGATAACGTAAGTTGAGGGTGCAGCGGCATa                                                                                           <  2:90306/150‑1 (MQ=255)
                                                             aCAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGAGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAAc                                                                                         <  1:247113/150‑1 (MQ=255)
                                                              cAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACa                                                                                        >  1:852486/1‑150 (MQ=255)
                                                              cAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACa                                                                                        >  2:628272/1‑150 (MQ=255)
                                                               aaaTAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACAt                                                                                       <  2:1280810/150‑1 (MQ=255)
                                                                    aCGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCa                                                                                  <  1:1476315/150‑1 (MQ=255)
                                                                     cGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAg                                                                                 >  2:1627621/1‑150 (MQ=255)
                                                                      gTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGa                                                                                >  2:651287/1‑150 (MQ=255)
                                                                      gTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGa                                                                                >  2:1681999/1‑150 (MQ=255)
                                                                        aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGGCCCCGATTCCGATTGCAGACGAAAGTAAAATACTCTATACTTGGAGCTCCGAATAACATTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              <  1:112237/150‑1 (MQ=255)
                                                                        aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              <  2:1077717/150‑1 (MQ=255)
                                                                        aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              >  2:731209/1‑150 (MQ=255)
                                                                                  aGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTa                                                                    <  2:487701/150‑1 (MQ=255)
                                                                                     tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 >  1:1128216/1‑150 (MQ=255)
                                                                                     tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 <  1:1495710/150‑1 (MQ=255)
                                                                                     tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 <  1:628272/150‑1 (MQ=255)
                                                                                      ctATGATGGAAATATTAAACGGCGAACGATTCAGAATGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACACTCACAAGTAGGGATAACGTAAGTTGAGGGTGCAGAGGCATAACATTGGCACAACAAAATCATTAACCt                                                                >  2:555126/1‑150 (MQ=255)
                                                                                         tgatgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                             <  1:541330/150‑1 (MQ=255)
                                                                                          gatgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTca                                                            <  2:911102/150‑1 (MQ=255)
                                                                                           atgGAAATATTTACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcac                                                           >  2:229305/1‑150 (MQ=255)
                                                                                            tgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcaca                                                          <  2:1554015/150‑1 (MQ=255)
                                                                                                  tatTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTCGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCAAAACATTTGCAGAACCACAAATTTAACCTTTCACaccacca                                                    >  2:336147/1‑149 (MQ=255)
                                                                                                     tAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                             <  1:978821/138‑1 (MQ=255)
                                                                                                     tAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGcc                                                 <  1:885863/150‑1 (MQ=255)
                                                                                                     tAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGcc                                                 >  2:1440053/1‑150 (MQ=255)
                                                                                                      aaCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGTAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCg                                                <  2:1143145/150‑1 (MQ=255)
                                                                                                      aaCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCg                                                <  2:754556/150‑1 (MQ=255)
                                                                                                        ccGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTc                                              >  1:934411/1‑150 (MQ=255)
                                                                                                           gCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAgg                                           >  1:1369628/1‑150 (MQ=255)
                                                                                                             gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                         >  1:1401746/1‑150 (MQ=255)
                                                                                                             gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                         >  2:1270027/1‑150 (MQ=255)
                                                                                                             gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGc                                          >  2:1468285/1‑149 (MQ=255)
                                                                                                             gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGc                                          <  1:1468285/149‑1 (MQ=255)
                                                                                                               aCGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGt                                       <  1:1613780/150‑1 (MQ=255)
                                                                                                                cGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGtt                                      <  2:715826/150‑1 (MQ=255)
                                                                                                                 gATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTg                                     <  1:1529447/150‑1 (MQ=255)
                                                                                                                    tCAGATTGCAGACGAAAGAAAAAAAGGCGCTCGGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAcc                                  <  1:622994/150‑1 (MQ=255)
                                                                                                                     cAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAcca                                 >  1:1091380/1‑150 (MQ=255)
                                                                                                                      aGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAccag                                >  1:671703/1‑150 (MQ=255)
                                                                                                                           gCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACcagccagc                           >  1:681252/1‑150 (MQ=255)
                                                                                                                           gCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACcagccagc                           >  1:588078/1‑150 (MQ=255)
                                                                                                                             aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGt                         <  1:1440053/150‑1 (MQ=255)
                                                                                                                                cGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGa                      >  2:1352411/1‑150 (MQ=255)
                                                                                                                                   aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGcc                   >  1:851705/1‑150 (MQ=255)
                                                                                                                                     gaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAg                 >  1:1243442/1‑150 (MQ=255)
                                                                                                                                      aaaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGc                >  1:614456/1‑150 (MQ=255)
                                                                                                                                          ttcggCGCTCCACGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCaaga            >  2:660591/4‑150 (MQ=255)
                                                                                                                                          ttcggCGCTCCACGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCaaga            >  1:341777/4‑150 (MQ=255)
                                                                                                                                             ggCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGTGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAagaaga         <  2:394308/150‑1 (MQ=255)
                                                                                                                                              gcgcTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGAc        <  2:83000/150‑1 (MQ=255)
                                                                                                                                                 cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTACCCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     >  2:783418/1‑150 (MQ=255)
                                                                                                                                                 cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     <  1:1681999/150‑1 (MQ=255)
                                                                                                                                                 cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     <  1:519321/150‑1 (MQ=255)
                                                                                                                                                 cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     <  1:717178/150‑1 (MQ=255)
                                                                                                                                                   ccGTGGAGCGCCGAATAACAGTCACAAGTTGGGATGACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATgg   <  1:57349/150‑1 (MQ=255)
                                                                                                                                                   ccGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATgg   >  1:408499/1‑150 (MQ=255)
                                                                                                                                                    cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  <  1:1333970/150‑1 (MQ=255)
                                                                                                                                                    cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  <  1:1219907/150‑1 (MQ=255)
                                                                                                                                                    cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  <  1:797781/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGT  >  NC_000913/4242397‑4242694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: