Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,242,547 T→C 5.2% intergenic (‑293/+79) xylE ← / ← malG D‑xylose transporter/maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,5470TC 5.2% 450.6 / 11.1 154intergenic (‑293/+79)xylE/malGD‑xylose transporter/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (70/76);  new base C (4/4);  total (74/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-02

Only 100 of 154 total aligned reads displayed.

TAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGT  >  NC_000913/4242399‑4242694
                                                                                                                                                    |                                                                                                                                                   
tAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTg                                                                                                                                                    <  2:1258552/150‑1 (MQ=255)
  aaaaCAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGa                                                                                                                                                  >  2:788303/1‑150 (MQ=255)
   aaaCAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAg                                                                                                                                                 <  1:839709/150‑1 (MQ=255)
   aaaCAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAg                                                                                                                                                 <  2:960423/150‑1 (MQ=255)
      cAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAgcgc                                                                                                                                              <  1:1572617/150‑1 (MQ=255)
        aTACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCg                                                                                                                                            >  1:1270363/1‑150 (MQ=255)
        aTACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCg                                                                                                                                            <  2:284149/150‑1 (MQ=255)
          aCTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGaa                                                                                                                                          >  2:1372962/1‑150 (MQ=255)
          aCTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCACGGAGCGccttt                                                                                                                                          <  2:1518077/150‑4 (MQ=255)
             aTTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCACGGAGCGcctttttt                                                                                                                                       <  2:880941/150‑7 (MQ=255)
               tGCCTTGACTAAGAGCACGAAATATAATTATAGTAATTGGTAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACa                                                                                                                                     <  1:1246391/150‑1 (MQ=255)
                gCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATAAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAg                                                                                                                                    <  2:697687/150‑1 (MQ=255)
                   gTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTca                                                                                                                                 >  2:1495710/1‑150 (MQ=255)
                   gTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTca                                                                                                                                 <  2:1098216/150‑1 (MQ=255)
                       cTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGTTCCGTGGAGCGCCGAATAACAGTCACAAg                                                                                                                             >  2:1718112/1‑150 (MQ=255)
                       cTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAg                                                                                                                             >  1:183360/1‑150 (MQ=255)
                       cTCAGAACACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAg                                                                                                                             >  1:1323701/1‑150 (MQ=255)
                         cAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGtt                                                                                                                           <  2:980115/150‑1 (MQ=255)
                          agagCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTg                                                                                                                          >  2:177170/1‑150 (MQ=255)
                             gCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGa                                                                                                                       <  2:775092/150‑1 (MQ=255)
                              cACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAAAAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGAt                                                                                                                      >  1:394308/1‑150 (MQ=255)
                               aCGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATa                                                                                                                     >  1:1077717/1‑150 (MQ=255)
                                cGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATaa                                                                                                                    >  1:727333/1‑150 (MQ=255)
                                cGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATaa                                                                                                                    <  2:1424470/150‑1 (MQ=255)
                                 gAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAc                                                                                                                   >  2:797781/1‑150 (MQ=255)
                                 gAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAc                                                                                                                   <  2:870729/150‑1 (MQ=255)
                                  aaaTAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATCATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACg                                                                                                                  <  2:114542/150‑1 (MQ=255)
                                  aaaGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACg                                                                                                                  >  2:541330/1‑150 (MQ=255)
                                   aaGAGAATTATCGTAAGTGGGAAAACAAATAAAGTAAAAATAATAAGCTCTATGATGGAATTATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGt                                                                                                                 <  1:976246/150‑1 (MQ=255)
                                        aaTTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGtt                                                                                                            >  1:1170915/1‑150 (MQ=255)
                                          ttATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCACCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGa                                                                                                          <  2:974913/150‑1 (MQ=255)
                                           tATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAg                                                                                                         >  2:717178/1‑150 (MQ=255)
                                             tCGTAAGTGGGAAAACAAATAACGTAAATTTAATAATCTCTATGATGGAAATATTATCCGGCGAACGATTCAGTTTGCAGACGATAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAggg                                                                                                       <  2:555678/150‑1 (MQ=255)
                                             tCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAggg                                                                                                       <  1:332848/150‑1 (MQ=255)
                                             tCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAggg                                                                                                       <  2:1024533/150‑1 (MQ=255)
                                                  aGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAg                                                                                                  >  1:499332/1‑150 (MQ=255)
                                                  aGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAg                                                                                                  <  1:44091/150‑1 (MQ=255)
                                                   gTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGc                                                                                                 >  1:83000/1‑150 (MQ=255)
                                                     gggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCgg                                                                                               >  2:1333970/1‑150 (MQ=255)
                                                      ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAATTATTAACCGGCGAACGATTCAGCTTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCCCAAGTTGGGATAACGTAAGTTGCGGGTGCAGCGGc                                                                                              >  2:1189814/1‑150 (MQ=255)
                                                      ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGc                                                                                              >  1:754556/1‑150 (MQ=255)
                                                      ggAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGc                                                                                              <  2:1219643/150‑1 (MQ=255)
                                                       gAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGACAAACAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCa                                                                                             <  1:257516/150‑1 (MQ=255)
                                                         aaaCAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCTAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGATGGGATAACGTAAGTTGAGGGTGCAGCGGCATa                                                                                           <  2:90306/150‑1 (MQ=255)
                                                          aaCAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATaa                                                                                          >  2:622994/1‑150 (MQ=255)
                                                            cAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACa                                                                                        >  1:715826/1‑150 (MQ=255)
                                                            cAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACa                                                                                        >  2:628272/1‑150 (MQ=255)
                                                             aaaTAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACAt                                                                                       <  2:1280810/150‑1 (MQ=255)
                                                                  aCGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCa                                                                                  <  1:1476315/150‑1 (MQ=255)
                                                                   cGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAg                                                                                 >  2:1627621/1‑150 (MQ=255)
                                                                    gTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGa                                                                                >  2:1681999/1‑150 (MQ=255)
                                                                    gTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGa                                                                                >  2:651287/1‑150 (MQ=255)
                                                                      aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGGCCCCGATTCCGATTGCAGACGAAAGTAAAATACTCTATACTTGGAGCTCCGAATAACATTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              <  1:112237/150‑1 (MQ=255)
                                                                      aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              >  2:731209/1‑150 (MQ=255)
                                                                      aaaaaTAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGaac                                                                              <  2:1077717/150‑1 (MQ=255)
                                                                                aGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTa                                                                    <  2:487701/150‑1 (MQ=255)
                                                                                aGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTa                                                                    >  2:829151/1‑150 (MQ=255)
                                                                                 gCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTaa                                                                   >  2:555605/1‑150 (MQ=255)
                                                                                   tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 >  1:1606752/1‑150 (MQ=255)
                                                                                   tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 >  1:1128216/1‑150 (MQ=255)
                                                                                   tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 <  1:1495710/150‑1 (MQ=255)
                                                                                   tctATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAAcc                                                                 >  1:1377586/1‑150 (MQ=255)
                                                                                       tgatgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                             <  1:541330/150‑1 (MQ=255)
                                                                                        gatgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTca                                                            <  2:911102/150‑1 (MQ=255)
                                                                                         atgGAAATATTTACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcac                                                           >  2:229305/1‑150 (MQ=255)
                                                                                         atgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcac                                                           >  2:673949/1‑150 (MQ=255)
                                                                                          tgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcaca                                                          <  2:1691603/150‑1 (MQ=255)
                                                                                          tgGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTcaca                                                          <  2:1554015/150‑1 (MQ=255)
                                                                                                   tAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTc                                                             <  1:978821/138‑1 (MQ=255)
                                                                                                   tAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGcc                                                 <  1:885863/150‑1 (MQ=255)
                                                                                                    aaCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGTAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCg                                                <  2:1143145/150‑1 (MQ=255)
                                                                                                    aaCCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCg                                                <  2:754556/150‑1 (MQ=255)
                                                                                                      ccGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTc                                              >  1:934411/1‑150 (MQ=255)
                                                                                                         gCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAgg                                           >  1:1369628/1‑150 (MQ=255)
                                                                                                           gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                         >  1:598988/1‑150 (MQ=255)
                                                                                                           gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGcc                                         >  2:1270027/1‑150 (MQ=255)
                                                                                                           gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGc                                          <  1:1468285/149‑1 (MQ=255)
                                                                                                           gAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGc                                          >  2:1468285/1‑149 (MQ=255)
                                                                                                              cGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGtt                                      <  2:715826/150‑1 (MQ=255)
                                                                                                               gATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTg                                     <  1:1529447/150‑1 (MQ=255)
                                                                                                                  tCAGATTGCAGACGAAAGAAAAAAAGGCGCTCGGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAcc                                  <  1:622994/150‑1 (MQ=255)
                                                                                                                   cAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAcca                                 >  1:1091380/1‑150 (MQ=255)
                                                                                                                    aGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGAccag                                >  1:671703/1‑150 (MQ=255)
                                                                                                                         gCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACcagccagc                           >  1:588078/1‑150 (MQ=255)
                                                                                                                           aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGt                         <  1:1440053/150‑1 (MQ=255)
                                                                                                                               gaaagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGCCCAGCCAGCGTTGAg                     >  1:619307/1‑150 (MQ=255)
                                                                                                                                 aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGcc                   >  1:851705/1‑150 (MQ=255)
                                                                                                                                 aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGcc                   <  1:170597/150‑1 (MQ=255)
                                                                                                                                   gaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAg                 >  1:1243442/1‑150 (MQ=255)
                                                                                                                                    gttattcggCGCTCCACGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGc                >  1:51057/8‑150 (MQ=255)
                                                                                                                                     ttattcggCGCTCCACGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCa               >  1:476505/7‑150 (MQ=255)
                                                                                                                                     aaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCa               <  1:555605/150‑1 (MQ=255)
                                                                                                                                           ggCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGTGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAagaaga         <  2:394308/150‑1 (MQ=255)
                                                                                                                                            gcgcTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGAc        <  2:83000/150‑1 (MQ=255)
                                                                                                                                               cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     <  1:651287/150‑1 (MQ=255)
                                                                                                                                               cTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGAt     <  1:1681999/150‑1 (MQ=255)
                                                                                                                                                 ccGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATgg   <  1:229305/150‑1 (MQ=255)
                                                                                                                                                  cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  >  2:6506/1‑150 (MQ=255)
                                                                                                                                                  cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  <  1:1219907/150‑1 (MQ=255)
                                                                                                                                                  cGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt  <  1:797781/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAAAAACAATACTATTGCCGTGACTCAGAGCACGAAAGAGAATTATCGTAAGTGGGAAAACAAATAACGTAAAAATAATAAGCTCTATGATGGAAATATTAACCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGT  >  NC_000913/4242399‑4242694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: