Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 446,786 C→T 5.9% intergenic (‑120/+29) yajR ← / ← cyoE putative transporter/protoheme IX farnesyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913446,7860CT 5.9% 430.8 / 7.0 134intergenic (‑120/+29)yajR/cyoEputative transporter/protoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base C (62/64);  new base T (4/4);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

Only 100 of 136 total aligned reads displayed.

CTCACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446637‑446934
                                                                                                                                                     |                                                                                                                                                    
ctcACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGcc                                                                                                                                                      <  2:1001890/150‑1 (MQ=255)
  ctacTGGCGTCATTTTATAATCTTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTATAGCGGTACACGAATTGGGTATACATGCTGATTATTTAGTAAATATTTCGATAGGGATATGTTTCTAATGCTAAGAATAAAGGTGCCGt                                                                                                                                                    <  2:1363326/147‑1 (MQ=255)
   aCCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTa                                                                                                                                                   <  1:48396/150‑1 (MQ=255)
    ccTGGCGTCATTTTATAATGGTTCATTACCACCTCTGTTTTAAATTAGCTAATAGTGTATAGGGGCAAACGAATGGGGTAAACATGCTTATTTTTTAGTAAATATTTCGATAGGGATATGTTTCTAATGCTAAGAAAAAATGTGCCGTAg                                                                                                                                                  <  2:1056225/150‑1 (MQ=255)
     cTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGc                                                                                                                                                 <  2:1409248/150‑1 (MQ=255)
      gggCGTCATTTTATATTCGTTCATTAACACCTCTGTTTTAAATTAGCGAATAGTGTAATGCGGCAATCGATTGGGGTAAACATGCTGATTTTTTAGTAAATATTTCGAGAGGGATTTTTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCa                                                                                                                                                <  1:270472/149‑1 (MQ=255)
         cGTCATTTTATAATCGTTCATTAACACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCt                                                                                                                                             <  1:918645/150‑1 (MQ=255)
          ttCATTTTATAATCGTTCATTACCCCCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtt                                                                                                                                            <  2:544236/149‑1 (MQ=255)
            cATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTTTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtttt                                                                                                                                          <  2:871990/150‑1 (MQ=255)
             aTTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttttt                                                                                                                                         <  2:484768/150‑1 (MQ=255)
             aTTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCttttt                                                                                                                                         <  2:1399013/150‑1 (MQ=255)
               tttATAATCGTACATTACCACCTCTCTTTTAAATTCGCGACTAGGGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGTATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                       <  2:256272/150‑3 (MQ=255)
               tttATAAGCGTTATTTAACACCTCTTTTTTAAAATGGCCATTATTGTAAAGCGGCTAACGTATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                       <  2:848318/150‑3 (MQ=255)
                ttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAAt                                                                                                                                      <  2:1320230/150‑1 (MQ=255)
                  ataATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATag                                                                                                                                    <  1:1441962/150‑1 (MQ=255)
                   taATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttcttag                                                                                                                                   <  2:1084560/150‑7 (MQ=255)
                     aTCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATagaga                                                                                                                                 >  2:1589078/1‑150 (MQ=255)
                         ttCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGATGtt                                                                                                                             >  1:156852/1‑150 (MQ=255)
                         ttCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGtt                                                                                                                             <  2:1411887/150‑1 (MQ=255)
                            aTTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGttttt                                                                                                                          >  1:1098040/1‑149 (MQ=255)
                              tACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGTGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGtt                                                                                                                        <  1:1392322/150‑1 (MQ=255)
                              tACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGtt                                                                                                                        >  2:8318/1‑150 (MQ=255)
                                 caccTCTGTTTTAAATTCGCTACTAGTGTAAAGCGGCAAACGAATGGGGTAAAGATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAcc                                                                                                                     <  1:305601/150‑1 (MQ=255)
                                  accTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACca                                                                                                                    <  2:48260/150‑1 (MQ=255)
                                   ccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTAATAGAGAGGTTTTGTTa                                                                                                                       >  2:45934/1‑145 (MQ=255)
                                         ttttAAATTCGCGACTATTGTAAAGCGGCTAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagca                                                                                                             <  1:1631248/150‑1 (MQ=255)
                                          tttAAATACGAGACTAGTGTAAAGCTGCAAACGAATGGGCTAAACATGCGGATTTTTAAGTAAATATTTAGAGATGGATATGTTTCTAATTCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcac                                                                                                            <  1:497172/150‑2 (MQ=255)
                                            tAAATTCGATACTAGTTTAAATCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcc                                                                                                          <  1:1040172/150‑1 (MQ=255)
                                             aaaTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcca                                                                                                         >  1:660865/1‑150 (MQ=255)
                                               aTTCGCGTCTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccagc                                                                                                       >  1:8986/1‑150 (MQ=255)
                                               aTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagccagc                                                                                                       <  1:584506/150‑1 (MQ=255)
                                                     gACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa                                                                                                 >  1:485958/1‑150 (MQ=255)
                                                      aCTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTAt                                                                                                >  1:69277/1‑150 (MQ=255)
                                                       cTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGTATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATg                                                                                               <  2:1492944/150‑1 (MQ=255)
                                                        tAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                              <  2:1585119/150‑1 (MQ=255)
                                                        tAGTGAAAAGCGGCAAACGAATGGGTTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                              <  2:341127/150‑1 (MQ=255)
                                                         aGTGTATAGCGGAAAACGAATTGGGTTAACATGCGGATTTTTTAGTTAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAACAGGTGCCGTAGCAGCTTTTTAATAGAGAGGTTTTGTTACCAGACAGCAGCCAGCAGCGTATGCg                                                                                             <  1:40692/150‑1 (MQ=255)
                                                         aGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCg                                                                                             >  1:208862/1‑150 (MQ=255)
                                                             tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCGTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTc                                                                                         >  2:1239171/1‑150 (MQ=255)
                                                               aaGCGGGAAACGAATGGGGTAAACATGCGTATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCg                                                                                       <  2:147167/150‑1 (MQ=255)
                                                               aaGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCg                                                                                       >  1:1577876/1‑150 (MQ=255)
                                                                aGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCgg                                                                                      >  1:729374/1‑150 (MQ=255)
                                                                aGCGGCAAACGAATGGGGGAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAACAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCgg                                                                                      <  2:1521911/150‑1 (MQ=255)
                                                                  cGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTa                                                                                    >  2:1384508/1‑150 (MQ=255)
                                                                    gCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTAcc                                                                                  >  2:302673/1‑150 (MQ=255)
                                                                     cAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                 >  2:541145/1‑150 (MQ=255)
                                                                      aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCAt                                                                                >  1:936310/1‑150 (MQ=255)
                                                                      aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGATAAGAAAAAAGGTGACTTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCATCCAGCAGCGTATGCGAGTCCGGTAACAt                                                                                >  1:348682/1‑150 (MQ=255)
                                                                       aaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATa                                                                               >  2:1321490/1‑150 (MQ=255)
                                                                       aaCAAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTATCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTAGTTACCACCCCGCAGCCAGCAGCGTATGCGAGTACGGTACAATa                                                                               >  2:697187/1‑150 (MQ=255)
                                                                        aCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCAAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATaa                                                                              >  1:185556/1‑150 (MQ=255)
                                                                           aaTGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAACAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAAt                                                                           >  1:1623301/1‑150 (MQ=255)
                                                                              ggggTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCaa                                                                        >  2:1304585/1‑150 (MQ=255)
                                                                               gggTAAACATGCGGATTTTTTAGTAAATATTTCTAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       <  1:417688/150‑1 (MQ=255)
                                                                                   aaaCATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGAc                                                                   <  1:747216/150‑1 (MQ=255)
                                                                                         gCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACatcatc                                                             <  1:39312/150‑1 (MQ=255)
                                                                                          cGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACAAAACAGCAGACAGCAGCGTATGATAGTCCTTTAACATAAAATCAACGGACAtcatca                                                            >  1:176332/1‑150 (MQ=255)
                                                                                            gATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCAcc                                                          <  2:1289566/150‑1 (MQ=255)
                                                                                              ttttttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGACAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATAACCga                                                        >  2:1025462/1‑150 (MQ=255)
                                                                                                ttttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTCTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCgaga                                                      >  2:1110297/1‑150 (MQ=255)
                                                                                                 tttAGTAAATATTTCAAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGagag                                                     <  2:185556/150‑1 (MQ=255)
                                                                                                    aGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGc                                                  >  2:745304/1‑150 (MQ=255)
                                                                                                      tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                <  1:1356962/150‑1 (MQ=255)
                                                                                                      tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                <  2:1098040/150‑1 (MQ=255)
                                                                                                      tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                >  2:1049040/1‑150 (MQ=255)
                                                                                                      tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                >  2:1705675/1‑150 (MQ=255)
                                                                                                          tatTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGAt                                            <  2:1714447/150‑1 (MQ=255)
                                                                                                           atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATg                                           >  1:799591/1‑150 (MQ=255)
                                                                                                           atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATg                                           <  1:1384508/150‑1 (MQ=255)
                                                                                                             ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATggg                                         >  1:522096/1‑149 (MQ=255)
                                                                                                             ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  1:8318/150‑1 (MQ=255)
                                                                                                             ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  2:570922/150‑1 (MQ=255)
                                                                                                             ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  2:660865/150‑1 (MQ=255)
                                                                                                               cgcgagGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGCTGGCga                                       <  2:190681/147‑1 (MQ=255)
                                                                                                               cGAGAGGTATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCga                                       <  2:502606/150‑1 (MQ=255)
                                                                                                                gagaGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCgat                                      <  2:729374/150‑1 (MQ=255)
                                                                                                                    gggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACTGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  >  1:815511/1‑150 (MQ=255)
                                                                                                                    gggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTCTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  <  1:1110297/150‑1 (MQ=255)
                                                                                                                      gATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATaga                                >  2:1575401/1‑150 (MQ=255)
                                                                                                                        tatGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAgaga                              <  2:8986/150‑1 (MQ=255)
                                                                                                                          tGTTTCTAATGCTAAGAAAATAGGTTCCGATGCACCTTTTTAATAGAGATGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGTTACCATAAAATCAACGGACATCATCATCGAGAGGGCAGTGATGGCGATTATAGAGAAg                            <  1:1113161/150‑1 (MQ=255)
                                                                                                                            tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGcc                          >  1:14589/1‑150 (MQ=255)
                                                                                                                            tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGcc                          <  1:1258596/150‑1 (MQ=255)
                                                                                                                              tCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGa                        >  1:1038824/1‑150 (MQ=255)
                                                                                                                              tCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGa                        >  1:1295250/1‑150 (MQ=255)
                                                                                                                                 aaTGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACa                     <  1:1548791/150‑1 (MQ=255)
                                                                                                                                   tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGc                   <  1:541145/150‑1 (MQ=255)
                                                                                                                                   tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGc                   <  1:534534/150‑1 (MQ=255)
                                                                                                                                       aaGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgc               <  2:14589/150‑1 (MQ=255)
                                                                                                                                        attaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         >  2:51575/4‑123 (MQ=255)
                                                                                                                                        aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg              >  2:105312/1‑150 (MQ=255)
                                                                                                                                        aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg              <  2:663075/150‑1 (MQ=255)
                                                                                                                                         gAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgc             >  1:25502/1‑150 (MQ=255)
                                                                                                                                          taaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgcg            >  1:1364412/2‑150 (MQ=255)
                                                                                                                                           aaaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgcgc           >  2:929910/1‑150 (MQ=255)
                                                                                                                                            aaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTAACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGATAGGGCAGTGATGGCGATGAGAGAGAAGCCGAACAGCTAGCGCGcc          >  1:1014728/1‑150 (MQ=255)
                                                                                                                                              aaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCa        >  1:1698740/1‑150 (MQ=255)
                                                                                                                                               aGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCTCGCCCAg       >  2:32021/1‑150 (MQ=255)
                                                                                                                                                   gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACAGCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTc   >  2:512087/1‑150 (MQ=255)
                                                                                                                                                    ccGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGAGCCCAGATTCt  >  1:350011/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTCACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446637‑446934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: