Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,263,307 G→C 5.4% R309G (CGT→GGT)  qorA ← quinone oxidoreductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,263,3070GC 5.4% 475.4 / 12.3 144R309G (CGT→GGT) qorAquinone oxidoreductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (63/73);  new base C (3/5);  total (66/78)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 144 total aligned reads displayed.

TGCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCA  >  NC_000913/4263160‑4263450
                                                                                                                                                   |                                                                                                                                               
tGCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCt                                                                                                                                               <  1:177499/150‑1 (MQ=255)
tGCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCt                                                                                                                                               <  2:79143/150‑1 (MQ=255)
 gcgcCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTg                                                                                                                                              >  1:540526/1‑150 (MQ=255)
 gcgcCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTg                                                                                                                                              <  2:540526/150‑1 (MQ=255)
 gcgcCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTg                                                                                                                                              >  1:1458119/1‑150 (MQ=255)
   gcCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTgcg                                                                                                                                            <  2:958380/150‑1 (MQ=255)
     ctctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCa                                                                                                                                          <  1:767942/150‑1 (MQ=255)
       ctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATc                                                                                                                                        >  2:1323097/1‑150 (MQ=255)
        tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCATCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATATCATGCGCACGCTGCGCATcc                                                                                                                                       >  2:1019248/1‑150 (MQ=255)
        tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtga                                                                                                                                       <  1:1560679/150‑3 (MQ=255)
        tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtga                                                                                                                                       <  2:246213/150‑3 (MQ=255)
        tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtga                                                                                                                                       <  1:827252/150‑3 (MQ=255)
        tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtga                                                                                                                                       <  1:884799/150‑3 (MQ=255)
            aGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCa                                                                                                                                   >  2:10448/1‑150 (MQ=255)
             gATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAg                                                                                                                                  >  2:1167881/1‑150 (MQ=255)
             gATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAg                                                                                                                                  >  2:442659/1‑150 (MQ=255)
               ttCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCTGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCg                                                                                                                                >  2:325152/1‑150 (MQ=255)
                    cGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGACCCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGatat                                                                                                                           >  2:408912/1‑150 (MQ=255)
                     gTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATAtt                                                                                                                          >  1:458106/1‑150 (MQ=255)
                       gCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTc                                                                                                                        <  2:1361016/150‑1 (MQ=255)
                              ccTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTgctgct                                                                                                                 >  1:1230247/1‑150 (MQ=255)
                              ccTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCAGAAATATccgc                                                                                                                     <  2:38713/146‑1 (MQ=255)
                               cTACATACAGCCGAACTATAAAAAGCAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCAGAAATATccgctgaag                                                                                                                <  1:1038859/150‑4 (MQ=255)
                                tacatacaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCATGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCg                                                                                                               >  1:1691372/1‑150 (MQ=255)
                                     gcaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGAc                                                                                                          >  2:1163025/2‑150 (MQ=255)
                                     acaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGAc                                                                                                          <  1:805064/150‑1 (MQ=255)
                                     acaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGAc                                                                                                          <  2:458106/150‑1 (MQ=255)
                                      caGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACa                                                                                                         >  2:1371065/1‑150 (MQ=255)
                                      caGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACa                                                                                                         >  1:651768/1‑150 (MQ=255)
                                      caGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAAACAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACa                                                                                                         >  1:841634/1‑150 (MQ=255)
                                        gCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATc                                                                                                       >  1:1467901/1‑150 (MQ=255)
                                         ccGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCGTATTGCTATTAGGGAATCAGCTCGGTGGATCCTTGCGTCGCCCGGATTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCg                                                                                                      <  1:79381/150‑1 (MQ=255)
                                         ccGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCg                                                                                                      >  2:684914/1‑150 (MQ=255)
                                          cGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCgc                                                                                                     >  2:63994/1‑149 (MQ=255)
                                          cGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGa                                                                                                     >  1:435196/1‑150 (MQ=255)
                                          cGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGa                                                                                                     <  2:1438227/150‑1 (MQ=255)
                                           gAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGAc                                                                                                    <  2:1458119/150‑1 (MQ=255)
                                            taCTATAAAAAGAAAGGGATTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTACAGAATCTCTTGCGCACGCTGCGCATGCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGAcc                                                                                                   <  2:1213941/149‑1 (MQ=255)
                                            aaCTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGAcc                                                                                                   <  1:1163025/150‑1 (MQ=255)
                                             aCTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCt                                                                                                  <  1:1280878/150‑1 (MQ=255)
                                                ataAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTaa                                                                                               >  2:64199/1‑150 (MQ=255)
                                                  aaaaaGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATc                                                                                             >  1:66775/1‑150 (MQ=255)
                                                   aaaaGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCACGCTGGAACCTTCCGTCGCCCGGCTTTCCAGAATCTCATGCGCACCCTGCGCATCCTTCAGCGGAGATTTCTGCTGCACGGCGACATCGACCTTAATca                                                                                            >  2:1688263/1‑150 (MQ=255)
                                                   aaaaGAAAGGGCTTCCCAGGTGGAAGCCCTATATCTTTTATGGAAACAGCACGCTGGAACCTTGCGTCGCCCGGCATTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGCCGACATCGACCTTAAtaa                                                                                            >  1:977601/1‑148 (MQ=255)
                                                    aaagaaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCGGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATcac                                                                                           <  1:914160/150‑1 (MQ=255)
                                                     aagaaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATcaca                                                                                          <  1:442659/150‑1 (MQ=255)
                                                       gaaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACAcc                                                                                        >  1:1377230/1‑150 (MQ=255)
                                                       gaaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACAcc                                                                                        >  2:1619140/1‑150 (MQ=255)
                                                         aagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACCCCGc                                                                                      >  2:439747/1‑150 (MQ=255)
                                                           gggCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATca                                                                                            >  1:1110501/1‑142 (MQ=255)
                                                           gggCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATca                                                                                            <  2:1110501/142‑1 (MQ=255)
                                                                 cccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATc                                                                              >  2:393932/1‑150 (MQ=255)
                                                                 cccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATc                                                                              <  1:339075/150‑1 (MQ=255)
                                                                   cAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCaa                                                                            >  1:917929/1‑150 (MQ=255)
                                                                     ggTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCGGGGTTTCCAGATTCTCATGCGCACGCTGCGCATCCTTCCGCGGATATTACTGCTGCTCGGCGACATCGACATTAATCACCCCGCTGGAAAGCAAag                                                                          >  1:1137456/1‑150 (MQ=255)
                                                                     ggTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAag                                                                          <  1:64199/150‑1 (MQ=255)
                                                                        ggAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAgaga                                                                       >  1:1365444/1‑150 (MQ=255)
                                                                          aaGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAAc                                                                     <  2:738170/150‑1 (MQ=255)
                                                                          aaGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAAc                                                                     <  2:66775/150‑1 (MQ=255)
                                                                             cccTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGt                                                                  <  1:19140/150‑1 (MQ=255)
                                                                              ccTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGtt                                                                 >  1:135576/1‑150 (MQ=255)
                                                                               cTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTc                                                                <  1:1348134/150‑1 (MQ=255)
                                                                               cTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGACTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTc                                                                >  2:663770/1‑150 (MQ=255)
                                                                                  tttCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCAtt                                                             >  2:308274/1‑150 (MQ=255)
                                                                                      ttttATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTg                                                         <  2:1230247/150‑1 (MQ=255)
                                                                                           tGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTc                                                    >  1:1257211/1‑150 (MQ=255)
                                                                                            ggAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCg                                                   >  1:966701/1‑150 (MQ=255)
                                                                                             gAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCgg                                                  <  2:1245338/150‑1 (MQ=255)
                                                                                                  agcagGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATACTTCTGCGTATATTTCTGCTGCTCGGCGAGATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTatt                                             <  1:105617/150‑3 (MQ=255)
                                                                                                    cagGCTTGAACCTTGCGTCGCCCGGCTTTCCTGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTGGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGACAACAGTTCATTACTGGCCTCGGTTAATtc                                           <  1:834217/150‑1 (MQ=255)
                                                                                                     agGCTGGAACCTTGCGTAGCCCGGCTTTCCAGAATCTCATGCGCCCGCGGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTACTTCACACCGCTGGCAAGCACCGAGAACAGGTCATTACTGGCCTCGGTTAATtcc                                          <  2:261150/150‑1 (MQ=255)
                                                                                                       gCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATtcctc                                        <  1:366202/150‑1 (MQ=255)
                                                                                                        cTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGCCATCGACCTTCATCCCACCGCTGGCAAGCAAAGAGATCAGGTCATTTCTGGCCTCGGTTAATtcctcc                                       >  1:274555/1‑150 (MQ=255)
                                                                                                        cTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATtcctcc                                       <  2:1672732/150‑1 (MQ=255)
                                                                                                        attgAACCTTTCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACAGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCTGTTAATtcatcc                                       >  1:1065498/4‑150 (MQ=255)
                                                                                                         tGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTccc                                      <  2:1257211/150‑1 (MQ=255)
                                                                                                            aaCCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCTGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCcgcg                                   <  1:628408/150‑1 (MQ=255)
                                                                                                             aCCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgt                                  <  1:186501/150‑1 (MQ=255)
                                                                                                             aCCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgt                                  <  1:1669409/150‑1 (MQ=255)
                                                                                                               cTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgtgg                                >  1:1302287/1‑150 (MQ=255)
                                                                                                               cTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgtgg                                >  2:413329/1‑150 (MQ=255)
                                                                                                                 tGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgtggtg                              <  2:135576/150‑1 (MQ=255)
                                                                                                                   cGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGat                            <  2:841634/150‑1 (MQ=255)
                                                                                                                   cGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGat                            >  2:772575/1‑150 (MQ=255)
                                                                                                                             cTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGc                  >  1:1199288/1‑150 (MQ=255)
                                                                                                                             cTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGc                  >  2:432742/1‑150 (MQ=255)
                                                                                                                              tttCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATATCTGCTGCTCGGAGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAAATCCTCCCGCGTGGGGATATAGCCTTGCa                 <  1:10448/150‑1 (MQ=255)
                                                                                                                               ttCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAg                <  1:393932/150‑1 (MQ=255)
                                                                                                                               ttCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAg                <  1:325152/150‑1 (MQ=255)
                                                                                                                                tCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAgg               <  1:1660770/150‑1 (MQ=255)
                                                                                                                                tCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAgg               <  1:1371065/150‑1 (MQ=255)
                                                                                                                                 ccAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAggg              <  1:684914/150‑1 (MQ=255)
                                                                                                                                   aGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGaa            <  1:1619140/150‑1 (MQ=255)
                                                                                                                                      aTCTCATGCGCACGCTGCGCATGCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACTCCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAggg         <  1:1688263/150‑1 (MQ=255)
                                                                                                                                      aTCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAggg         <  1:703409/150‑1 (MQ=255)
                                                                                                                                      aTCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAggg         <  1:626455/150‑1 (MQ=255)
                                                                                                                                        ctcATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCg       >  2:1310594/1‑150 (MQ=255)
                                                                                                                                        ctcATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCg       <  2:966701/150‑1 (MQ=255)
                                                                                                                                            tGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTc   >  2:1379119/1‑150 (MQ=255)
                                                                                                                                             gcgcACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTca  <  2:265006/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCA  >  NC_000913/4263160‑4263450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: