Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 446,787 G→A 5.9% intergenic (‑121/+28) yajR ← / ← cyoE putative transporter/protoheme IX farnesyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913446,7870GA 5.9% 430.2 / 8.7 135intergenic (‑121/+28)yajR/cyoEputative transporter/protoheme IX farnesyltransferase
Reads supporting (aligned to +/- strand):  ref base G (62/64);  new base A (4/4);  total (67/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

Only 100 of 135 total aligned reads displayed.

CACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446639‑446934
                                                                                                                                                    |                                                                                                                                                   
ctacTGGCGTCATTTTATAATCTTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTATAGCGGTACACGAATTGGGTATACATGCTGATTATTTAGTAAATATTTCGATAGGGATATGTTTCTAATGCTAAGAATAAAGGTGCCGt                                                                                                                                                    <  2:1363326/147‑1 (MQ=255)
   cTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGc                                                                                                                                                 <  2:1409248/150‑1 (MQ=255)
    gggCGTCATTTTATATTCGTTCATTAACACCTCTGTTTTAAATTAGCGAATAGTGTAATGCGGCAATCGATTGGGGTAAACATGCTGATTTTTTAGTAAATATTTCGAGAGGGATTTTTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCa                                                                                                                                                <  1:270472/149‑1 (MQ=255)
       cGTCATTTTATAATCGTTCATTAACACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCt                                                                                                                                             <  1:918645/150‑1 (MQ=255)
        ttCATTTTATAATCGTTCATTACCCCCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtt                                                                                                                                            <  2:544236/149‑1 (MQ=255)
          cATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTTTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtttt                                                                                                                                          <  2:871990/150‑1 (MQ=255)
          cATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtttt                                                                                                                                          <  1:1459992/150‑1 (MQ=255)
            ttttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTa                                                                                                                                        >  1:663075/1‑150 (MQ=255)
             tttATAATCGTACATTACCACCTCTCTTTTAAATTCGCGACTAGGGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGTATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                       <  2:256272/150‑3 (MQ=255)
             tttATAAGCGTTATTTAACACCTCTTTTTTAAAATGGCCATTATTGTAAAGCGGCTAACGTATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttc                                                                                                                                       <  2:848318/150‑3 (MQ=255)
              ttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAAt                                                                                                                                      <  2:1313363/150‑1 (MQ=255)
              ttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAAt                                                                                                                                      >  2:39312/1‑150 (MQ=255)
              ttATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAAt                                                                                                                                      <  2:1320230/150‑1 (MQ=255)
                ataATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATag                                                                                                                                    <  1:1441962/150‑1 (MQ=255)
                 taATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCTACGGCACCttttttcttag                                                                                                                                   <  2:1084560/150‑7 (MQ=255)
                 taATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATaga                                                                                                                                   >  2:1403954/1‑150 (MQ=255)
                       ttCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGATGtt                                                                                                                             >  1:156852/1‑150 (MQ=255)
                       ttCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGtt                                                                                                                             <  2:1411887/150‑1 (MQ=255)
                         cATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGtttt                                                                                                                           >  2:1548791/1‑150 (MQ=255)
                          aTTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGttttt                                                                                                                          >  1:1098040/1‑149 (MQ=255)
                            tACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGTGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGtt                                                                                                                        <  1:1392322/150‑1 (MQ=255)
                            tACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGtt                                                                                                                        >  2:8318/1‑150 (MQ=255)
                              ccaccTCTTTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAAAGCTAAGAAAAAAGGCGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAc                                                                                                                      <  1:354797/150‑1 (MQ=255)
                              ccaccTCTGTTTTAAATTCGCGACTAGTTTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAc                                                                                                                      <  1:29230/150‑1 (MQ=255)
                                accTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACca                                                                                                                    <  2:48260/150‑1 (MQ=255)
                                 ccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTAATAGAGAGGTTTTGTTa                                                                                                                       <  1:45934/145‑1 (MQ=255)
                                 ccTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTAATAGAGAGGTTTTGTTa                                                                                                                       >  2:45934/1‑145 (MQ=255)
                                      gTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagc                                                                                                              >  1:857144/1‑150 (MQ=255)
                                       ttttAAATTCGCGACTATTGTAAAGCGGCTAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagca                                                                                                             <  1:1631248/150‑1 (MQ=255)
                                        tttAAATACGAGACTAGTGTAAAGCTGCAAACGAATGGGCTAAACATGCGGATTTTTAAGTAAATATTTAGAGATGGATATGTTTCTAATTCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcac                                                                                                            <  1:497172/150‑2 (MQ=255)
                                          tAAATTCGATACTAGTTTAAATCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcc                                                                                                          <  1:1040172/150‑1 (MQ=255)
                                           aaaTTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACAcagcagcca                                                                                                         >  1:660865/1‑150 (MQ=255)
                                                   gACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa                                                                                                 >  1:485958/1‑150 (MQ=255)
                                                     cTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGTATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATg                                                                                               <  2:1492944/150‑1 (MQ=255)
                                                     cTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATg                                                                                               <  2:1380272/150‑1 (MQ=255)
                                                      tAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc                                                                                              <  2:1585119/150‑1 (MQ=255)
                                                       aGTGTATAGCGGAAAACGAATTGGGTTAACATGCGGATTTTTTAGTTAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAACAGGTGCCGTAGCAGCTTTTTAATAGAGAGGTTTTGTTACCAGACAGCAGCCAGCAGCGTATGCg                                                                                             <  1:40692/150‑1 (MQ=255)
                                                       aGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCg                                                                                             >  1:208862/1‑150 (MQ=255)
                                                       aGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATCCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCACCAGCGTATGCg                                                                                             >  1:752398/1‑150 (MQ=255)
                                                           tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTc                                                                                         >  2:500856/1‑150 (MQ=255)
                                                           tAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCGTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTc                                                                                         >  2:1239171/1‑150 (MQ=255)
                                                            aaaGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTcc                                                                                        >  2:1066089/1‑150 (MQ=255)
                                                             aaGCGGGAAACGAATGGGGTAAACATGCGTATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCg                                                                                       <  2:147167/150‑1 (MQ=255)
                                                             aaGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCg                                                                                       >  1:1577876/1‑150 (MQ=255)
                                                              aGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCgg                                                                                      >  1:729374/1‑150 (MQ=255)
                                                                cGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTa                                                                                    >  2:1384508/1‑150 (MQ=255)
                                                                  gCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTAcc                                                                                  >  2:302673/1‑150 (MQ=255)
                                                                   cAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                 >  2:541145/1‑150 (MQ=255)
                                                                   cAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCa                                                                                 >  2:534534/1‑150 (MQ=255)
                                                                    aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCAt                                                                                >  1:936310/1‑150 (MQ=255)
                                                                    aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCAt                                                                                >  2:146267/1‑150 (MQ=255)
                                                                    aaaCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGATAAGAAAAAAGGTGACTTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCATCCAGCAGCGTATGCGAGTCCGGTAACAt                                                                                >  1:348682/1‑150 (MQ=255)
                                                                     aaCAAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTATCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTAGTTACCACCCCGCAGCCAGCAGCGTATGCGAGTACGGTACAATa                                                                               >  2:697187/1‑150 (MQ=255)
                                                                      aCGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCAAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATaa                                                                              >  1:185556/1‑150 (MQ=255)
                                                                             gggTAAACATGCGGATTTTTTAGTAAATATTTCTAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAAc                                                                       <  1:417688/150‑1 (MQ=255)
                                                                                 aaaCATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGAc                                                                   <  1:747216/150‑1 (MQ=255)
                                                                                        cGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACAAAACAGCAGACAGCAGCGTATGATAGTCCTTTAACATAAAATCAACGGACAtcatca                                                            >  1:176332/1‑150 (MQ=255)
                                                                                          gATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCAcc                                                          <  2:1289566/150‑1 (MQ=255)
                                                                                               tttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAACAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGagag                                                     <  2:1623301/150‑1 (MQ=255)
                                                                                               tttAGTAAATATTTCAAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGagag                                                     <  2:185556/150‑1 (MQ=255)
                                                                                                ttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAgg                                                    >  1:570922/1‑150 (MQ=255)
                                                                                                  aGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGc                                                  >  2:745304/1‑150 (MQ=255)
                                                                                                    tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                <  1:1356962/150‑1 (MQ=255)
                                                                                                    tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                <  2:1098040/150‑1 (MQ=255)
                                                                                                    tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                >  2:1049040/1‑150 (MQ=255)
                                                                                                    tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAg                                                >  2:1705675/1‑150 (MQ=255)
                                                                                                        tatTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGAt                                            <  2:1714447/150‑1 (MQ=255)
                                                                                                         atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATg                                           <  1:1384508/150‑1 (MQ=255)
                                                                                                           ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  1:8318/150‑1 (MQ=255)
                                                                                                           ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  2:570922/150‑1 (MQ=255)
                                                                                                           ttCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  2:660865/150‑1 (MQ=255)
                                                                                                             cgcgagGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGCTGGCga                                       <  2:190681/147‑1 (MQ=255)
                                                                                                             cGAGAGGTATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCga                                       <  2:502606/150‑1 (MQ=255)
                                                                                                              gagaGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCgat                                      <  2:729374/150‑1 (MQ=255)
                                                                                                                  gggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACTGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  >  1:815511/1‑150 (MQ=255)
                                                                                                                  gggATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTCTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATa                                  <  1:1110297/150‑1 (MQ=255)
                                                                                                                    gATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATaga                                >  2:1575401/1‑150 (MQ=255)
                                                                                                                     atatGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGAGGATagag                               >  1:976729/1‑150 (MQ=255)
                                                                                                                      tatGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAgaga                              <  2:8986/150‑1 (MQ=255)
                                                                                                                        tGTTTCTAATGCTAAGAAAATAGGTTCCGATGCACCTTTTTAATAGAGATGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGTTACCATAAAATCAACGGACATCATCATCGAGAGGGCAGTGATGGCGATTATAGAGAAg                            <  1:1113161/150‑1 (MQ=255)
                                                                                                                          tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGcc                          <  1:1258596/150‑1 (MQ=255)
                                                                                                                          tttCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGcc                          >  1:14589/1‑150 (MQ=255)
                                                                                                                            tCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGa                        >  1:1038824/1‑150 (MQ=255)
                                                                                                                            tCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGa                        >  1:1295250/1‑150 (MQ=255)
                                                                                                                                 tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGc                   <  1:541145/150‑1 (MQ=255)
                                                                                                                                 tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGc                   <  1:534534/150‑1 (MQ=255)
                                                                                                                                      attaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGc                                         <  1:51575/120‑1 (MQ=255)
                                                                                                                                      aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg              <  1:1589078/150‑1 (MQ=255)
                                                                                                                                      aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg              >  2:105312/1‑150 (MQ=255)
                                                                                                                                      aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcg              <  2:663075/150‑1 (MQ=255)
                                                                                                                                       ttaaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgc             >  2:1189242/3‑150 (MQ=255)
                                                                                                                                       gAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgc             >  1:25502/1‑150 (MQ=255)
                                                                                                                                        taaaaaGGTGCTACGGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgcg            >  1:1364412/2‑150 (MQ=255)
                                                                                                                                         aaaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTgcgcgc           >  2:929910/1‑150 (MQ=255)
                                                                                                                                          aaaaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTAACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGATAGGGCAGTGATGGCGATGAGAGAGAAGCCGAACAGCTAGCGCGcc          >  1:1014728/1‑150 (MQ=255)
                                                                                                                                            aaGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCa        >  1:1698740/1‑150 (MQ=255)
                                                                                                                                             aGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCTCGCCCAg       >  2:32021/1‑150 (MQ=255)
                                                                                                                                                 gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGGCATCATCACCGAGAGGGCAGTGATGGCGCTGATAGAGAAGCCGACCAGCTTGCGCGCCCACAtt  >  2:1045357/1‑149 (MQ=255)
                                                                                                                                                 gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACAGCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTc   >  2:512087/1‑150 (MQ=255)
                                                                                                                                                  ccGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGAGCCCAGATTCt  >  1:350011/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACCTGGCGTCATTTTATAATCGTTCATTACCACCTCTGTTTTAAATTCGCGACTAGTGTAAAGCGGCAAACGAATGGGGTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGACATCATCACCGAGAGGGCAGTGATGGCGATGATAGAGAAGCCGAACAGCTTGCGCGCCCAGATTCT  >  NC_000913/446639‑446934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: