BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,530	A→T	5.9%	I614I (ATT→ATA)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,530	0	A	T	5.9%	376.4 / 7.4	129	I614I (ATT→ATA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (57/64); new base T (6/2); total (63/66)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAA  >  NC_000913/4285381‑4285678

aCCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGa                                                                                                                                                      <  2:566138/150‑1 (MQ=255)

  cGTTACCGACTCGCATCGGGCAATTGGGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGGTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAAt                                                                                                                                                    <  2:1043998/150‑1 (MQ=255)

    ttACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATAc                                                                                                                                                  <  1:234569/150‑1 (MQ=255)

    ttACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCAGCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATAc                                                                                                                                                  <  1:421385/150‑1 (MQ=255)

      aCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACgg                                                                                                                                                <  2:1214484/150‑1 (MQ=255)

       ccGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgc                                                                                                                                               <  2:817304/150‑1 (MQ=255)

        cGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgcg                                                                                                                                              <  1:1556039/150‑1 (MQ=255)

        cGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgcg                                                                                                                                              <  2:803388/150‑1 (MQ=255)

         gACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgcgc                                                                                                                                             >  2:262114/1‑150 (MQ=255)

              gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAt                                                                                                                                        <  1:1334172/150‑1 (MQ=255)

               cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtt                                                                                                                                       >  1:854702/1‑150 (MQ=255)

               cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtt                                                                                                                                       >  1:583634/1‑150 (MQ=255)

                 tCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtttt                                                                                                                                     <  1:1717943/150‑1 (MQ=255)

                    ggCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGcc                                                                                                                                  >  1:1300787/1‑150 (MQ=255)

                      cAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCgg                                                                                                                                >  2:878266/1‑150 (MQ=255)

                             gggCTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGGCGAGGTATCGCCCAGGGTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGt                                                                                                                         <  1:356160/150‑1 (MQ=255)

                                ttACGATGGCATCGCGATAACCTGCTTCTCTTCAAGCAGCTTCTCGACTACTCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGtttt                                                                                                                      <  2:610210/150‑1 (MQ=255)

                                 tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                             >  1:101818/1‑126 (MQ=255)

                                 tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                             >  1:101819/1‑126 (MQ=255)

                                 tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                             <  2:101819/126‑1 (MQ=255)

                                   cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAgg                                                                                                                   <  1:776944/150‑1 (MQ=255)

                                    gATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCTAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGc                                                                                                                  <  2:1417798/150‑1 (MQ=255)

                                       ggCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAgg                                                                                                               >  2:1647714/1‑150 (MQ=255)

                                       ggCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAgg                                                                                                               <  1:1289960/150‑1 (MQ=255)

                                        gCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAggg                                                                                                              <  2:388698/150‑1 (MQ=255)

                                         cATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGa                                                                                                             >  1:1430249/1‑150 (MQ=255)

                                         cATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGa                                                                                                             >  1:1182748/1‑150 (MQ=255)

                                          aTCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAg                                                                                                            >  1:967599/1‑150 (MQ=255)

                                                 tAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGAGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTcc                                                                                                     >  1:1442032/1‑150 (MQ=255)

                                                   gCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                                                   >  1:1223886/1‑150 (MQ=255)

                                                   gCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                                                   >  1:700116/1‑150 (MQ=255)

                                                     cTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTg                                                                                                 <  2:942765/150‑1 (MQ=255)

                                                      tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCggg                                                                                                                          >  1:603780/1‑124 (MQ=255)

                                                      tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgc                                                                                                >  1:480398/1‑150 (MQ=255)

                                                      ggCTTCTCTTCAAGATGCTTCTCGTCTAGGCCAGGATCGGCAAGCTTCGAGGTATCGCCCAGGTTGCTGGTATCGCACGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgc                                                                                                <  2:283587/149‑1 (MQ=255)

                                                        cTTCTCTTCCAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcag                                                                                              <  2:1523541/150‑1 (MQ=255)

                                                         ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                             <  2:949859/150‑1 (MQ=255)

                                                         ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                             >  1:1681921/1‑150 (MQ=255)

                                                          tctcTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagca                                                                                            >  2:446008/1‑150 (MQ=255)

                                                            tctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCTGGTTTTAGGCAGGGAGTCGGTCAAGTGCAGCACg                                                                                          >  2:1530577/1‑150 (MQ=255)

                                                            gctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGTGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACg                                                                                          <  1:1687617/149‑1 (MQ=255)

                                                             ctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                                                         >  1:1610181/1‑150 (MQ=255)

                                                             ctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                                                         >  2:1634148/1‑150 (MQ=255)

                                                              ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTc                                                                                        <  1:1115628/150‑1 (MQ=255)

                                                                  agcagcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGc                                                                                    >  2:695256/1‑150 (MQ=255)

                                                                   gcagcTTCTCGCCTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCg                                                                                   <  1:1271809/150‑1 (MQ=255)

                                                                             gACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCgg                                                                         <  2:1249543/150‑1 (MQ=255)

                                                                              aCTACGACAGGATCGGAAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTAGCCAGCggg                                                                        >  1:187210/1‑150 (MQ=255)

                                                                                tACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGcc                                                                      <  2:337106/150‑1 (MQ=255)

                                                                                  cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCaa                                                                    >  2:588309/1‑150 (MQ=255)

                                                                                  cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCaa                                                                    >  1:1432361/1‑150 (MQ=255)

                                                                                    ccAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCATGTCTGGCGTCGCCAGCGGGCCAAtc                                                                  <  2:700116/150‑1 (MQ=255)

                                                                                    ccAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtc                                                                  <  2:19171/150‑1 (MQ=255)

                                                                                      aGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtctc                                                                <  1:1634148/150‑1 (MQ=255)

                                                                                        gATCGGCAAGCGTCGCGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCtt                                                              <  1:692634/150‑1 (MQ=255)

                                                                                          tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                            <  2:636489/150‑1 (MQ=255)

                                                                                          tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                            <  1:872223/150‑1 (MQ=255)

                                                                                           cGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTAc                                                           <  2:1223886/150‑1 (MQ=255)

                                                                                             gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGc                                                         >  1:1339434/1‑150 (MQ=255)

                                                                                              cAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGcc                                                        >  1:87063/1‑149 (MQ=255)

                                                                                                cgCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                                                      <  2:1309314/149‑1 (MQ=255)

                                                                                                aGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                                                      >  1:1476075/1‑150 (MQ=255)

                                                                                                aGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGCTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGACCAATCTCTTTACGCAcc                                                      >  2:1055181/1‑150 (MQ=255)

                                                                                                aGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCAGCAATTATGCGCAGTATACGGCGCATTATTTTGCCGGAGCGCGTTGTAGGCAGGGAGTCGGTGCAGTGCAGCACGACTCGCGTCGCCAGCGGGCCAAGCACTTTACGccac                                                      >  1:699570/1‑147 (MQ=255)

                                                                                                     cGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGtt                                                 <  2:583634/150‑1 (MQ=255)

                                                                                                     agAGGTATCGCCCAGGTTGCTTGTATCGCCCGCCGCAATTTTGAGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGtt                                                 <  2:854702/149‑1 (MQ=255)

                                                                                                      gAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTg                                                >  1:1665269/1‑150 (MQ=255)

                                                                                                      gAGGTATCGCCCAGGTTGCTGGTATCGACCGCCGCAATTATGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCCGCACGTCAGGCGTCGCCCGCGCGCCAATCACTTTACGCACCCAGTTg                                                >  1:116661/1‑150 (MQ=255)

                                                                                                       aGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGc                                               >  2:542375/1‑150 (MQ=255)

                                                                                                        ggTAGCGCCCAGGTTGCTGGTATCGCCCGCCTCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCGGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCg                                              <  2:1191004/150‑1 (MQ=255)

                                                                                                          tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGACTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                            <  1:507667/150‑1 (MQ=255)

                                                                                                          tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCTGTGCAGCACGTCTGGCGTCGCCCGCGGTCCAATCTCTTTACGCGCACATTTGCggc                                            >  2:301996/1‑149 (MQ=255)

                                                                                                          tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                            >  1:1708398/1‑150 (MQ=255)

                                                                                                          aaTCGCCCAGGTTGCTGGAATCGCCCGCCGCAATTATGCGCAGAATAAGGCGCATAATATTTCCGGAGAGGGTTTTAGGCAGTGAGGAGGTCCAGTGCAGCACGTCTGGCGTCGCCCGCGGGCGATGCTCTTTACGCCCCCAGTTGCGGa                                            >  1:859447/2‑150 (MQ=255)

                                                                                                              gCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTc                                        <  1:1626535/150‑1 (MQ=255)

                                                                                                              gCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTc                                        >  2:349999/1‑150 (MQ=255)

                                                                                                                ccAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGACCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTg                                      >  1:173634/1‑150 (MQ=255)

                                                                                                                 cAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACACAGTTGCGGACTTCTGc                                     >  2:499976/1‑150 (MQ=255)

                                                                                                                   ggTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                             >  2:663141/1‑92 (MQ=255)

                                                                                                                        cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                              <  2:1681921/150‑1 (MQ=255)

                                                                                                                        cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                              <  2:1585751/150‑1 (MQ=255)

                                                                                                                        cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGGGTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                              >  1:995566/1‑150 (MQ=255)

                                                                                                                            tATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTg                          >  1:1381279/1‑150 (MQ=255)

                                                                                                                             aTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTgg                         <  2:1267281/150‑1 (MQ=255)

                                                                                                                             aTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTgg                         <  2:187210/150‑1 (MQ=255)

                                                                                                                             aTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTAGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGGCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCATTTGCGGACTTCTGCGTACAGTTCTgg                         <  1:1564210/150‑1 (MQ=255)

                                                                                                                               cgcccgccGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTg                       <  2:1707417/150‑1 (MQ=255)

                                                                                                                                gcccgccGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGa                      >  1:1576957/1‑150 (MQ=255)

                                                                                                                                  ccgccgCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACg                    >  2:1501376/1‑150 (MQ=255)

                                                                                                                                   cggcaaaaTTATGCGCCGTATTCTGCGCAAAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACgg                   >  2:476819/7‑150 (MQ=255)

                                                                                                                                    ggcaaaaTTATGCGCCGTATTCTGCGCAAAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGt                  >  1:8853/6‑150 (MQ=255)

                                                                                                                                      cgcAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtc                <  2:1566302/150‑1 (MQ=255)

                                                                                                                                       gcAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcc               <  2:20816/150‑1 (MQ=255)

                                                                                                                                        cAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcct              >  2:622774/1‑150 (MQ=255)

                                                                                                                                         aaTTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcctc             <  1:816985/150‑1 (MQ=255)

                                                                                                                                           ttttGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTccc           <  2:1576957/150‑1 (MQ=255)

                                                                                                                                           ttttGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGGTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTccc           <  1:1032876/150‑1 (MQ=255)

                                                                                                                                             atGCGCCGTATTCTGCGCAAAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCg         >  1:524415/2‑150 (MQ=255)

                                                                                                                                                   aGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTa   >  1:591026/1‑150 (MQ=255)

                                                                                                                                                    gAATACGGCGCATATTTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGCCTGCTGCGGACAGTTCTGGTGACGGTTCCGCCCCGGGATTaa  >  1:754921/1‑150 (MQ=255)

ACCGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAA  >  NC_000913/4285381‑4285678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`