BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,285,533	A→T	5.2%	R613R (CGT→CGA) ‡	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,533	0	A	T	5.2%	380.4 / 6.3	131	R613R (CGT→CGA) ‡	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (59/65); new base T (5/2); total (64/67)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 132 total aligned reads displayed.

GTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCG  >  NC_000913/4285384‑4285681

gTTACCGCCTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATa                                                                                                                                                      >  2:872223/1‑150 (MQ=255)

 ttACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATAc                                                                                                                                                     <  1:234569/150‑1 (MQ=255)

   aCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACgg                                                                                                                                                   <  2:1214484/150‑1 (MQ=255)

     cGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgcg                                                                                                                                                 <  2:803388/150‑1 (MQ=255)

     cGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGgcg                                                                                                                                                 <  1:1556039/150‑1 (MQ=255)

       aCTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCa                                                                                                                                               <  2:516787/150‑1 (MQ=255)

           gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAt                                                                                                                                           <  1:1334172/150‑1 (MQ=255)

            cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtt                                                                                                                                          >  1:583634/1‑150 (MQ=255)

            cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtt                                                                                                                                          >  1:854702/1‑150 (MQ=255)

              tCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtttt                                                                                                                                        <  1:1717943/150‑1 (MQ=255)

              tCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAAtttt                                                                                                                                        >  1:1707417/1‑150 (MQ=255)

                 ggCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGcc                                                                                                                                     >  1:1300787/1‑150 (MQ=255)

                 ggCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGcc                                                                                                                                     <  2:400890/150‑1 (MQ=255)

                          gggCTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGGCGAGGTATCGCCCAGGGTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGt                                                                                                                            <  1:356160/150‑1 (MQ=255)

                             ttACGATGGCATCGCGATAACCTGCTTCTCTTCAAGCAGCTTCTCGACTACTCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGtttt                                                                                                                         <  2:610210/150‑1 (MQ=255)

                              tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                                <  2:101818/126‑1 (MQ=255)

                              tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                                >  1:101819/1‑126 (MQ=255)

                              tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                                <  2:101819/126‑1 (MQ=255)

                              tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCCGTATTCTgcgc                                                                                                                                                >  1:101818/1‑126 (MQ=255)

                               aCGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAg                                                                                                                       <  1:878266/150‑1 (MQ=255)

                                cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAgg                                                                                                                      <  1:776944/150‑1 (MQ=255)

                                 gATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCTAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGc                                                                                                                     <  2:1417798/150‑1 (MQ=255)

                                    ggCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAgg                                                                                                                  <  1:1289960/150‑1 (MQ=255)

                                     gCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAggg                                                                                                                 <  2:388698/150‑1 (MQ=255)

                                      cATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGa                                                                                                                >  1:1182748/1‑150 (MQ=255)

                                      cATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGa                                                                                                                >  1:1430249/1‑150 (MQ=255)

                                              tAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGAGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTcc                                                                                                        >  1:1442032/1‑150 (MQ=255)

                                                gCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                                                      >  1:1223886/1‑150 (MQ=255)

                                                  cTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTg                                                                                                    <  2:942765/150‑1 (MQ=255)

                                                   tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCggg                                                                                                                             <  2:603780/124‑1 (MQ=255)

                                                   tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgc                                                                                                   >  1:480398/1‑150 (MQ=255)

                                                   ggCTTCTCTTCAAGATGCTTCTCGTCTAGGCCAGGATCGGCAAGCTTCGAGGTATCGCCCAGGTTGCTGGTATCGCACGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgc                                                                                                   <  2:283587/149‑1 (MQ=255)

                                                     cTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcag                                                                                                 <  2:216195/150‑1 (MQ=255)

                                                      ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                                >  1:1681921/1‑150 (MQ=255)

                                                      ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                                >  1:1585751/1‑150 (MQ=255)

                                                      ttCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                                <  2:949859/150‑1 (MQ=255)

                                                       tctcTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagca                                                                                               >  2:446008/1‑150 (MQ=255)

                                                         tctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCTGGTTTTAGGCAGGGAGTCGGTCAAGTGCAGCACg                                                                                             >  2:1530577/1‑150 (MQ=255)

                                                          ctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                                                            >  2:1634148/1‑150 (MQ=255)

                                                          ctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                                                            >  1:1610181/1‑150 (MQ=255)

                                                           ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTc                                                                                           <  1:1115628/150‑1 (MQ=255)

                                                              aaGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTgg                                                                                        <  1:1647714/150‑1 (MQ=255)

                                                                 cagcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGt                                                                                     >  2:1626535/1‑150 (MQ=255)

                                                                  agcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTc                                                                                    >  2:1701745/1‑150 (MQ=255)

                                                                       ctcGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAg                                                                               <  2:1539072/150‑1 (MQ=255)

                                                                          gACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCgg                                                                            <  2:1249543/150‑1 (MQ=255)

                                                                             tACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGcc                                                                         <  2:337106/150‑1 (MQ=255)

                                                                               cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCaa                                                                       >  1:1432361/1‑150 (MQ=255)

                                                                               cGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCaa                                                                       >  2:588309/1‑150 (MQ=255)

                                                                                 ccAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCATGTCTGGCGTCGCCAGCGGGCCAAtc                                                                     <  2:700116/150‑1 (MQ=255)

                                                                                 ccAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtc                                                                     <  2:19171/150‑1 (MQ=255)

                                                                                  cAGGATCGGCAAGCGCCGAGGTATCGCCCAGGATGCTGGTATCGCCCGCCGCAATTATGCGCAGAATACCGCGCATAAATTTGCCGGAGCGGGCTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAAtct                                                                    <  1:219694/150‑1 (MQ=255)

                                                                                    ggATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                                                  >  1:1518363/1‑150 (MQ=255)

                                                                                     gATCGGCAAGCGTCGCGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCtt                                                                 <  1:692634/150‑1 (MQ=255)

                                                                                       tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                               <  1:7517/150‑1 (MQ=255)

                                                                                       tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                               <  2:636489/150‑1 (MQ=255)

                                                                                       tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                               <  1:872223/150‑1 (MQ=255)

                                                                                       tCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTAGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                                               <  1:446008/150‑1 (MQ=255)

                                                                                          gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGc                                                            >  1:1339434/1‑150 (MQ=255)

                                                                                          gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGc                                                            >  2:1418466/1‑150 (MQ=255)

                                                                                             aGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGCTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGACCAATCTCTTTACGCAcc                                                         >  2:1055181/1‑150 (MQ=255)

                                                                                             aGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCAGCAATTATGCGCAGTATACGGCGCATTATTTTGCCGGAGCGCGTTGTAGGCAGGGAGTCGGTGCAGTGCAGCACGACTCGCGTCGCCAGCGGGCCAAGCACTTTACGccac                                                         >  1:699570/1‑147 (MQ=255)

                                                                                                  agAGGTATCGCCCAGGTTGCTTGTATCGCCCGCCGCAATTTTGAGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGtt                                                    <  2:854702/149‑1 (MQ=255)

                                                                                                   gAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTg                                                   >  1:1665269/1‑150 (MQ=255)

                                                                                                   gAGGTATCGCCCAGGTTGCTGGTATCGACCGCCGCAATTATGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCCGCACGTCAGGCGTCGCCCGCGCGCCAATCACTTTACGCACCCAGTTg                                                   >  1:116661/1‑150 (MQ=255)

                                                                                                    aGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGc                                                  >  2:542375/1‑150 (MQ=255)

                                                                                                     ggTAGCGCCCAGGTTGCTGGTATCGCCCGCCTCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCGGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCg                                                 <  2:1191004/150‑1 (MQ=255)

                                                                                                       tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGACTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                               <  1:507667/150‑1 (MQ=255)

                                                                                                       tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCTGTGCAGCACGTCTGGCGTCGCCCGCGGTCCAATCTCTTTACGCGCACATTTGCggc                                               >  2:301996/1‑149 (MQ=255)

                                                                                                       tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                               >  1:1708398/1‑150 (MQ=255)

                                                                                                       tATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATATTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                                               >  1:859445/1‑150 (MQ=255)

                                                                                                       aaTCGCCCAGGTTGCTGGAATCGCCCGCCGCAATTATGCGCAGAATAAGGCGCATAATATTTCCGGAGAGGGTTTTAGGCAGTGAGGAGGTCCAGTGCAGCACGTCTGGCGTCGCCCGCGGGCGATGCTCTTTACGCCCCCAGTTGCGGa                                               >  1:859447/2‑150 (MQ=255)

                                                                                                          cGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTAGCCTGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACtt                                            >  2:328662/1‑150 (MQ=255)

                                                                                                           gCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTc                                           <  1:1626535/150‑1 (MQ=255)

                                                                                                             ccAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGACCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTg                                         >  1:173634/1‑150 (MQ=255)

                                                                                                              cAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACACAGTTGCGGACTTCTGc                                        >  2:499976/1‑150 (MQ=255)

                                                                                                               aGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCg                                       >  2:640984/1‑150 (MQ=255)

                                                                                                                ggTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                                <  1:663141/92‑1 (MQ=255)

                                                                                                                ggTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgcagc                                                                                                >  2:663141/1‑92 (MQ=255)

                                                                                                                     cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                                 <  2:1681921/150‑1 (MQ=255)

                                                                                                                     cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                                 <  2:1585751/150‑1 (MQ=255)

                                                                                                                     cTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGGGTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGt                                 >  1:995566/1‑150 (MQ=255)

                                                                                                                         tATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTg                             >  1:1381279/1‑150 (MQ=255)

                                                                                                                         tATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTg                             <  2:72528/150‑1 (MQ=255)

                                                                                                                          ttCGCCGGCCGCAATTTTGCGCAGACTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTgg                            <  1:542875/149‑1 (MQ=255)

                                                                                                                          aTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTgg                            <  2:1267281/150‑1 (MQ=255)

                                                                                                                          aTCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTAGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGGCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCATTTGCGGACTTCTGCGTACAGTTCTgg                            <  1:1564210/150‑1 (MQ=255)

                                                                                                                            cgcccgccGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTg                          <  2:1707417/150‑1 (MQ=255)

                                                                                                                               ccgccgCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACg                       >  2:1501376/1‑150 (MQ=255)

                                                                                                                                 ggcaaaaTTATGCGCCGTATTCTGCGCAAAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGt                     >  1:8853/6‑150 (MQ=255)

                                                                                                                                   cgcAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtc                   <  2:1566302/150‑1 (MQ=255)

                                                                                                                                    gcAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcc                  <  2:20816/150‑1 (MQ=255)

                                                                                                                                    gcAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCAGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTtcc                  <  2:1257707/150‑1 (MQ=255)

                                                                                                                                        ttttGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGGTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTccc              <  1:1032876/150‑1 (MQ=255)

                                                                                                                                                aGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTa      >  1:591026/1‑150 (MQ=255)

                                                                                                                                                 gAATACGGCGCATATTTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGCCTGCTGCGGACAGTTCTGGTGACGGTTCCGCCCCGGGATTaa     >  1:754921/1‑150 (MQ=255)

                                                                                                                                                   aTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGc   <  1:1280025/150‑1 (MQ=255)

                                                                                                                                                   aTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGc   >  2:1129163/1‑150 (MQ=255)

                                                                                                                                                   aTACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGc   >  2:957330/1‑150 (MQ=255)

                                                                                                                                                    tACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCg  <  2:87063/150‑1 (MQ=255)

GTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCG  >  NC_000913/4285384‑4285681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`