Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,297,195 A→T 5.3% intergenic (‑70/+24) yjcO ← / ← fdhF Sel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,297,1950AT 5.3% 376.0 / 8.4 132intergenic (‑70/+24)yjcO/fdhFSel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit
Reads supporting (aligned to +/- strand):  ref base A (69/56);  new base T (4/3);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-02

Only 100 of 135 total aligned reads displayed.

AAATACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCG  >  NC_000913/4297046‑4297343
                                                                                                                                                     |                                                                                                                                                    
aaaTACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGaa                                                                                                                                                      >  1:185084/1‑150 (MQ=255)
    aCTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAagga                                                                                                                                                  <  1:206097/150‑1 (MQ=255)
     cTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAaggag                                                                                                                                                 <  2:785682/150‑1 (MQ=255)
     cTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAaggag                                                                                                                                                 <  2:1371467/150‑1 (MQ=255)
         cTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTg                                                                                                                                             <  1:902892/150‑1 (MQ=255)
           gCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTa                                                                                                                                           >  2:689964/1‑150 (MQ=255)
           gCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTa                                                                                                                                           >  2:365972/1‑150 (MQ=255)
            ccAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGttt                                                                                                                                          <  2:1635612/150‑3 (MQ=255)
            ccAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCTTTCGGAGGCTGttt                                                                                                                                          <  2:9413/150‑3 (MQ=255)
             cAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGa                                                                                                                                         >  2:653359/1‑150 (MQ=255)
              aGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGaa                                                                                                                                        <  1:494883/150‑1 (MQ=255)
               ggCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGTCTGTAGaaa                                                                                                                                       >  1:1504357/1‑150 (MQ=255)
                gCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAg                                                                                                                                      <  1:282559/150‑1 (MQ=255)
                gCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAg                                                                                                                                      >  1:196943/1‑150 (MQ=255)
                  tCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGa                                                                                                                                    >  2:932522/1‑150 (MQ=255)
                   cGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGAc                                                                                                                                   <  1:966318/150‑1 (MQ=255)
                    ggAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACg                                                                                                                                  <  2:205884/150‑1 (MQ=255)
                         cGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAtt                                                                                                                             <  1:1400353/150‑1 (MQ=255)
                         cGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAtt                                                                                                                             >  1:603858/1‑150 (MQ=255)
                         cGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTAtt                                                                                                                             >  1:1570406/1‑150 (MQ=255)
                              gCGTGGGCAAAGATTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGcc                                                                                                                        <  1:612875/150‑1 (MQ=255)
                               cGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCa                                                                                                                       >  2:464755/1‑150 (MQ=255)
                                 tGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAGTGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGt                                                                                                                     >  1:1409264/1‑150 (MQ=255)
                                  gggCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTg                                                                                                                    >  2:240954/1‑150 (MQ=255)
                                     cAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCg                                                                                                                 <  2:649540/150‑1 (MQ=255)
                                     cAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCg                                                                                                                 >  1:1332952/1‑150 (MQ=255)
                                           aTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTcgc                                                                                                           >  1:1306815/1‑150 (MQ=255)
                                           aTGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTAGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGACGCTTcgc                                                                                                           >  1:1313718/1‑150 (MQ=255)
                                                aaaaaaaaCATCAATGCGATAATTTTTATCATTTTGATAATACGATTGGGTAGGGACTAACCAGTATAAAGAGTTCGAATGGAAAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAgg                                                                                                      <  1:1451844/150‑1 (MQ=255)
                                                aaaaaCAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAgg                                                                                                      <  1:1224538/150‑1 (MQ=255)
                                                     caacaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGt                                                                                                 >  2:145448/1‑150 (MQ=255)
                                                     caacaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGt                                                                                                 >  2:44415/1‑150 (MQ=255)
                                                     caacaTCAATGCGAAAATTTTTTTCATTTTTATAATACGATTGGGTAGGGACTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGt                                                                                                 <  2:738787/150‑1 (MQ=255)
                                                       acaTCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGttt                                                                                               >  2:1005750/1‑150 (MQ=255)
                                                           cAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCaa                                                                                           >  1:21610/1‑150 (MQ=255)
                                                           cAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCaa                                                                                           >  2:494415/1‑150 (MQ=255)
                                                            aaTGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAAc                                                                                          <  1:150611/150‑1 (MQ=255)
                                                            aaTGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAAc                                                                                          >  2:548581/1‑150 (MQ=255)
                                                             aTGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACTGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCaaat                                                                                         >  2:1694159/1‑148 (MQ=255)
                                                                cGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgt                                                                                      <  1:1211242/150‑1 (MQ=255)
                                                                cGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACttgt                                                                                      <  1:462205/150‑1 (MQ=255)
                                                                   tAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTtgttgt                                                                                   >  2:1657118/1‑150 (MQ=255)
                                                                     aTTTTTTTCATTTTGATAATACGGTTGGGGAGGGAGTAACCAGTATAAAGAGATCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGGATTACGCCAGTGCCGCTTCGCGCCGGCGAGTTTTCCCCTTGTTGTAc                                                                                 >  1:1021547/1‑150 (MQ=255)
                                                                     aTTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGACTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTAc                                                                                 >  1:1543334/1‑150 (MQ=255)
                                                                      tttttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACt                                                                                <  2:65657/150‑1 (MQ=255)
                                                                      tttttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACt                                                                                <  2:21610/150‑1 (MQ=255)
                                                                       ttttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTc                                                                               <  2:1687874/150‑1 (MQ=255)
                                                                        tttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCg                                                                              >  2:1388298/1‑150 (MQ=255)
                                                                         ttttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTAAGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGt                                                                             >  1:1071626/1‑150 (MQ=255)
                                                                          tttCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTc                                                                            <  1:1657118/150‑1 (MQ=255)
                                                                            tCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCaa                                                                          >  2:543230/1‑150 (MQ=255)
                                                                             cATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAAt                                                                         <  2:87691/150‑1 (MQ=255)
                                                                              aTTTTGATAATAAGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATc                                                                        <  2:1304373/150‑1 (MQ=255)
                                                                               ttttGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCa                                                                       >  1:1594122/1‑150 (MQ=255)
                                                                               ttttGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCa                                                                       >  1:1080980/1‑150 (MQ=255)
                                                                                tttGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCAc                                                                      >  1:269947/1‑150 (MQ=255)
                                                                                 ttGATAATACGATTGGGTAGTGAGTAACCAGAATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGGCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACg                                                                     <  2:516591/150‑1 (MQ=255)
                                                                                   gATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTa                                                                   >  2:1538167/1‑150 (MQ=255)
                                                                                    ataataCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTAc                                                                  >  2:797996/1‑150 (MQ=255)
                                                                                     taataCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACt                                                                 <  2:1504357/150‑1 (MQ=255)
                                                                                        taCGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCt                                                              >  2:338301/1‑150 (MQ=255)
                                                                                           gATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcgg                                                           >  2:1662565/1‑150 (MQ=255)
                                                                                            aTTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcggc                                                          <  2:177610/150‑1 (MQ=255)
                                                                                              tGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcggcgg                                                        <  2:980854/150‑1 (MQ=255)
                                                                                              tGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTcggcgg                                                        <  2:1438690/150‑1 (MQ=255)
                                                                                                ggTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGgcggcg                                                      <  1:145448/150‑1 (MQ=255)
                                                                                                   aGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGgcgcgc                                                   >  1:1683536/1‑150 (MQ=255)
                                                                                                    gggAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCt                                                  <  1:148239/150‑1 (MQ=255)
                                                                                                     ggAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTg                                                 >  1:1211828/1‑150 (MQ=255)
                                                                                                          aaCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcgg                                            <  1:1662565/150‑1 (MQ=255)
                                                                                                          aaCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTAATGCTCGGCGGCGCGCTgatcgg                                            >  1:863456/1‑150 (MQ=255)
                                                                                                           aCCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggc                                           <  2:1217581/150‑1 (MQ=255)
                                                                                                            ccAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcg                                          >  1:24511/1‑150 (MQ=255)
                                                                                                              aGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgat                                        >  2:1107811/1‑150 (MQ=255)
                                                                                                               ttataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                       <  1:932522/149‑1 (MQ=255)
                                                                                                                tataAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatcg                                      >  2:1446337/1‑150 (MQ=255)
                                                                                                                      gagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                       >  1:793827/1‑143 (MQ=255)
                                                                                                                      gagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTgatcggcgatc                                       <  2:793827/143‑1 (MQ=255)
                                                                                                                      gagTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCTGCTCGa                                <  1:1388298/150‑1 (MQ=255)
                                                                                                                        gTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGAcg                              <  2:603858/150‑1 (MQ=255)
                                                                                                                           cGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGTGCGGCTCGAcgcgc                           >  2:499668/1‑149 (MQ=255)
                                                                                                                             aaTGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAAc                         >  1:730927/1‑150 (MQ=255)
                                                                                                                               tGGATAAAAAAACAGCCTCCGAAAGGAGGCAGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGTCGCTCGGCTCGACGCGAACgg                       >  2:949204/1‑150 (MQ=255)
                                                                                                                                 gATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcg                     <  2:1543334/150‑1 (MQ=255)
                                                                                                                                  aTAAAAACACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCATCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcgc                    <  1:93926/150‑1 (MQ=255)
                                                                                                                                  aTAAAAAAACCGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGGCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGgcgc                    <  1:177220/150‑1 (MQ=255)
                                                                                                                                    ctttctaCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAg                  >  1:788066/7‑150 (MQ=255)
                                                                                                                                    aaaaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAg                  >  2:256660/1‑150 (MQ=255)
                                                                                                                                      aaaaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTa                >  2:115964/1‑150 (MQ=255)
                                                                                                                                       aaaaCAGCCTCCGAAAGGAGGCTGTAGATAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTTCTCGGCGGCGCGCTGATCGGCGATCGGCTCGAAGCGAACGGCGCAGTAt               >  1:293183/1‑150 (MQ=255)
                                                                                                                                        ctaCAGCCTCCTTTCGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAtt              >  2:712171/3‑150 (MQ=255)
                                                                                                                                        aaaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAtt              >  1:437215/1‑150 (MQ=255)
                                                                                                                                         aaCAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTAttt             <  1:494415/150‑1 (MQ=255)
                                                                                                                                          ccAGCCTCCGAACGGAGGCTGTAGACAGGACGGACTTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTg            <  1:674508/149‑1 (MQ=255)
                                                                                                                                           cAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGt           >  1:1094584/1‑150 (MQ=255)
                                                                                                                                            aGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGTCGATCGGCTCGCCGCGAACGGCGCAGTATATGTa          >  1:628093/1‑150 (MQ=255)
                                                                                                                                            aGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCACGACGCGAACGGCGCAGTATTTGTa          >  1:1174977/1‑150 (MQ=255)
                                                                                                                                                 ccGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCg     >  1:447519/1‑150 (MQ=255)
                                                                                                                                                  cGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCgg    <  1:653359/150‑1 (MQ=255)
                                                                                                                                                    aaaGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCg  <  1:1107811/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATACTGGCTGCCAGGCTCGGAGTCGTTGGCGTGGGCAAAGAATGTCAAAAACAACATCAATGCGATAATTTTTTTCATTTTGATAATACGATTGGGTAGGGAGTAACCAGTATAAAGAGTTCGAATGGATAAAAAAACAGCCTCCGAAAGGAGGCTGTAGAAAGGACGGTATTACGCCAGTGCCGCTTCGCGCAGGCGAGTTTTCAACTTGTTGTACTCGTCAATCACGTACTGCTCGGCGGCGCGCTGATCGGCGATCGGCTCGACGCGAACGGCGCAGTATTTGTACTCCGGCG  >  NC_000913/4297046‑4297343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑