Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,310,961 G→T 5.8% S7* (TCG→TAG)  alsA ← D‑allose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,310,9610GT 5.8% 355.6 / 6.3 112S7* (TCG→TAG) alsAD‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (48/57);  new base T (3/4);  total (51/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

GCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTAT  >  NC_000913/4310812‑4311110
                                                                                                                                                     |                                                                                                                                                     
gCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCg                                                                                                                                                       >  1:911963/1‑150 (MQ=255)
 cTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGa                                                                                                                                                      >  1:845697/1‑150 (MQ=255)
  tCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGat                                                                                                                                                     <  2:360064/150‑1 (MQ=255)
   cATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGata                                                                                                                                                    >  2:84864/1‑150 (MQ=255)
   cATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGata                                                                                                                                                    >  2:371662/1‑150 (MQ=255)
       tATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGatatata                                                                                                                                                <  1:404989/150‑1 (MQ=255)
        aTTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTGATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGatatatat                                                                                                                                               >  1:1476659/1‑150 (MQ=255)
          tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAGTGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATgg                                                                                                                                             >  2:1365013/1‑150 (MQ=255)
          tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATgg                                                                                                                                             >  2:1641555/1‑150 (MQ=255)
          tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACAAGGATAAACCGTTAAATTAACCGACTTTAATGCTTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATgg                                                                                                                                             >  2:1293129/1‑150 (MQ=255)
           ccGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGc                                                                                                                                            <  1:447213/150‑1 (MQ=255)
            cGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCg                                                                                                                                           >  2:1469814/1‑150 (MQ=255)
            cGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCg                                                                                                                                           >  2:346673/1‑150 (MQ=255)
             ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGt                                                                                                                                          >  1:1437044/1‑150 (MQ=255)
             ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCACGCCATCGATATATATGGCGt                                                                                                                                          <  2:665884/150‑1 (MQ=255)
              gATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCAAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTg                                                                                                                                         <  2:1090535/150‑1 (MQ=255)
               aTAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTgg                                                                                                                                        <  1:266135/150‑1 (MQ=255)
                    aCTTTCATTAGCGTTGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCATGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCACGCCATATATATCGATGGCggggatcgg                                                                                                                                   <  2:311998/150‑6 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATTCTTGTATTTCACCAGGATAAACCGTTATATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  2:1016330/150‑1 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCATGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  1:1255303/150‑1 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  1:571447/150‑1 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  1:564610/150‑1 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  1:1631978/150‑1 (MQ=255)
                           ttAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  2:1016328/150‑1 (MQ=255)
                             aGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACctc                                                                                                                          >  1:1111940/1‑150 (MQ=255)
                              gCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            <  2:393492/147‑1 (MQ=255)
                              gCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                                                                                            >  1:393492/1‑147 (MQ=255)
                                 tGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTg                                                                                                                      <  2:1361202/150‑1 (MQ=255)
                                  ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGc                                                                                                                     <  1:1484879/150‑1 (MQ=255)
                                    aTTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCAc                                                                                                                   >  1:1244194/1‑150 (MQ=255)
                                         cccGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                                                                                              >  2:1172516/1‑150 (MQ=255)
                                         cccGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCACCGCCTTCGATATATATGGCGTTGCCATAAATAACCTCTCTGCACGAAat                                                                                                              >  1:1107456/1‑150 (MQ=255)
                                         cccGCGCCATTTTCTCCTAGTAATGCATGAATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                                                                                              >  1:1095200/1‑150 (MQ=255)
                                                  ttttCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtatta                                                                                                     <  2:845697/150‑1 (MQ=255)
                                                     tctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTg                                                                                                  <  1:266464/150‑1 (MQ=255)
                                                     tctcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTg                                                                                                  <  1:1641555/150‑1 (MQ=255)
                                                       tcCTAGTAATGCATGTATTTCTCCTGGATAAACCGTTATATTAACCGACTTTAATGCGTGTACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGcc                                                                                                <  1:1563123/150‑1 (MQ=255)
                                                       tcCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATATCCTCTCTGCACGAAATATCGTATTATTGcc                                                                                                <  1:1604651/150‑1 (MQ=255)
                                                        ccTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCt                                                                                               <  1:84864/150‑1 (MQ=255)
                                                        ccTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGGGGCCATAAATAACCTCTCTGCACGAAATATGGTATTATTGGCt                                                                                               >  2:1675023/1‑150 (MQ=255)
                                                         cTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTg                                                                                              >  2:175619/1‑150 (MQ=255)
                                                          tAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGa                                                                                             >  2:748316/1‑150 (MQ=255)
                                                           aGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGAt                                                                                            >  2:1531895/1‑150 (MQ=255)
                                                           aGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGAGCACAGGACCAAAGGACTTGCCGAACACCGCCATCGATATATATCGCGTGGCAATAAATAACCTCTCTGCACGAAATATCGTATTATTGCGTGAt                                                                                            >  1:174118/1‑150 (MQ=255)
                                                            gTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATg                                                                                           <  1:175619/150‑1 (MQ=255)
                                                             tAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATgg                                                                                          <  1:647801/150‑1 (MQ=255)
                                                             tAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATgg                                                                                          <  1:801278/150‑1 (MQ=255)
                                                             tAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATgg                                                                                          <  1:346673/150‑1 (MQ=255)
                                                             tAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATgg                                                                                          <  1:1469814/150‑1 (MQ=255)
                                                              aaTGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGc                                                                                         <  2:1201701/150‑1 (MQ=255)
                                                                 gCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTAc                                                                                      <  1:1351922/150‑1 (MQ=255)
                                                                 gCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTAc                                                                                      <  2:1152238/150‑1 (MQ=255)
                                                                  cATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACa                                                                                     >  1:241808/1‑150 (MQ=255)
                                                                  cATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACa                                                                                     >  1:1274464/1‑150 (MQ=255)
                                                                    tGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACAtt                                                                                   >  1:1287675/1‑150 (MQ=255)
                                                                            aCCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGt                                                                           >  2:1627/1‑150 (MQ=255)
                                                                             ccAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTc                                                                          <  2:1423925/150‑1 (MQ=255)
                                                                                ggATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTAc                                                                       >  2:1539390/1‑150 (MQ=255)
                                                                                 gATAAACCGTTAAATTAACCGACTTTAATGAGTTAACCGGAACAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACg                                                                      <  2:1107456/150‑1 (MQ=255)
                                                                                      aCCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAg                                                                 <  1:1365013/150‑1 (MQ=255)
                                                                                       ccGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAgg                                                                >  2:1508989/1‑150 (MQ=255)
                                                                                       ccGATAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGTACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAgg                                                                <  1:656573/150‑1 (MQ=255)
                                                                                         gTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGtt                                                              >  1:546188/1‑150 (MQ=255)
                                                                                         gTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGtt                                                              <  1:1293129/150‑1 (MQ=255)
                                                                                            aaaTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTAtt                                                           <  2:1244194/150‑1 (MQ=255)
                                                                                             aaTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTAttt                                                          <  2:1476659/150‑1 (MQ=255)
                                                                                              aTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTAtttt                                                         >  1:677425/1‑150 (MQ=255)
                                                                                              aTTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTAtttt                                                         <  2:1698088/150‑1 (MQ=255)
                                                                                               ttAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTttg                                                        >  1:622971/1‑150 (MQ=255)
                                                                                                 aaCCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTttgtt                                                      <  1:1508989/150‑1 (MQ=255)
                                                                                                    cGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGc                                                   >  2:1441611/1‑150 (MQ=255)
                                                                                                    cGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGc                                                   >  2:420367/1‑150 (MQ=255)
                                                                                                      aCTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCg                                                 <  1:787953/150‑1 (MQ=255)
                                                                                                          tAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATa                                             <  2:1111940/150‑1 (MQ=255)
                                                                                                          tAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATa                                             <  1:702547/150‑1 (MQ=255)
                                                                                                             tGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGa                                          >  1:1647338/1‑150 (MQ=255)
                                                                                                              gCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                                         <  1:1288452/150‑1 (MQ=255)
                                                                                                                 tGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACt                                      >  2:482410/1‑150 (MQ=255)
                                                                                                                   aaCCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTc                                    <  1:1490713/150‑1 (MQ=255)
                                                                                                                      cGGACCAAATGACTTGCAGATCCCATCCATCTATATATATGGCGTGGCCATAAATAACCTCTCAGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACTTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCAtt                                 <  1:105760/150‑1 (MQ=255)
                                                                                                                      cGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCAtt                                 >  2:721370/1‑150 (MQ=255)
                                                                                                                       ggACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTa                                >  2:342597/1‑150 (MQ=255)
                                                                                                                        gaacAAAGGACTTTCCGATCACCGCCATCGATATAAATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTTGCCGGATAAGACACTTCATTAg                               <  2:466932/147‑1 (MQ=255)
                                                                                                                          ccAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGca                             >  2:1376599/1‑150 (MQ=255)
                                                                                                                            aaaGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatc                           <  2:911963/150‑1 (MQ=255)
                                                                                                                            aaaGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatc                           >  2:1198436/1‑150 (MQ=255)
                                                                                                                            aaaGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatc                           >  2:728793/1‑150 (MQ=255)
                                                                                                                             aaGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatca                          <  1:490120/150‑1 (MQ=255)
                                                                                                                              aGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcat                         <  2:992891/150‑1 (MQ=255)
                                                                                                                               tgACTTGCAGATCACCGACATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAATTATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatc                        <  2:1095200/149‑1 (MQ=255)
                                                                                                                                gACTTGCCGATCCCCTCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatca                       <  1:1000699/150‑1 (MQ=255)
                                                                                                                                   ttGCCGATCCCCGCCATCTATATATATGTCTTGGCAATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATcc                    <  2:677425/150‑1 (MQ=255)
                                                                                                                                     gCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCgg                  >  1:118532/1‑150 (MQ=255)
                                                                                                                                       cGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGca                <  1:1273548/150‑1 (MQ=255)
                                                                                                                                       atggccacGCCATATATATCGATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGca                >  2:174963/8‑150 (MQ=255)
                                                                                                                                         ggccacGCCATATATATCGATGGCGGGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcatc              >  2:1596689/6‑150 (MQ=255)
                                                                                                                                          tCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcatca             <  2:546188/150‑1 (MQ=255)
                                                                                                                                            cccGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCAtt           >  2:731573/1‑150 (MQ=255)
                                                                                                                                            cccGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCAtt           >  2:978770/1‑150 (MQ=255)
                                                                                                                                                 cATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGtt      >  1:1330069/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTAT  >  NC_000913/4310812‑4311110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: