Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,089 A→G 6.3% intergenic (‑47/+12) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0890AG 6.3% 412.9 / 13.8 142intergenic (‑47/+12)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base A (61/72);  new base G (7/2);  total (68/74)
Fisher's exact test for biased strand distribution p-value = 8.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 146 total aligned reads displayed.

AGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGA  >  NC_000913/4311940‑4312238
                                                                                                                                                     |                                                                                                                                                     
agagGGTTTTCAATACAACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGaa                                                                                                                                                       <  1:1642413/150‑1 (MQ=255)
 gagGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCATGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                                                                                      >  1:1352632/1‑150 (MQ=255)
   gggTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                                                                    >  1:711310/1‑150 (MQ=255)
    tgTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                                                                                   <  2:96663/149‑1 (MQ=255)
     gTTTTCAATACGACAGCATATTCGGTGGCAGCAACATCGCGGGGTGAAAACATTAAGCCCAGGAGGGTGCCGCGGAAATATCTCAGATAGTTATGCATAATAATGTTCTCACGGTAAGTGGACAGGTATCTCCGCGATGGCGGAATTAcc                                                                                                                                                  <  1:288069/150‑1 (MQ=255)
     gTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                                                                  <  1:1702624/150‑1 (MQ=255)
     gTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                                                                  <  1:1377151/150‑1 (MQ=255)
     gTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGGGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                                                                  <  2:1055406/150‑1 (MQ=255)
       tttCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                                                                                <  2:1539545/150‑2 (MQ=255)
          cAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCttttt                                                                                                                                             >  2:324188/1‑150 (MQ=255)
           aaTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCAGAATTACCTTTTTc                                                                                                                                            <  2:1598023/150‑1 (MQ=255)
            aTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                                                                           <  1:1441286/150‑1 (MQ=255)
            aTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                                                                           <  2:647538/150‑1 (MQ=255)
             tACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                                                                          <  2:207774/150‑1 (MQ=255)
               cGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAt                                                                                                                                        <  2:965806/150‑1 (MQ=255)
                 aCAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAttt                                                                                                                                      <  2:630060/150‑1 (MQ=255)
                 aCAGCATATTCGGCGGCAACAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAttt                                                                                                                                      >  2:843761/1‑150 (MQ=255)
                   aGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCa                                                                                                                                    >  2:653293/1‑150 (MQ=255)
                    gCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg                                                                                                                                   <  1:1405867/150‑1 (MQ=255)
                    gCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg                                                                                                                                   >  1:1025954/1‑150 (MQ=255)
                     cctatTCGGCTGCAGCAAAAGCGCTGGTTGACAACATTCAGCCCACGCGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                                                                                  <  1:208972/148‑1 (MQ=255)
                      atatTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                                                                                 <  2:1619751/150‑1 (MQ=255)
                      atatTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                                                                                 >  1:1257760/1‑150 (MQ=255)
                         ttCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                                                                              >  2:1490662/1‑150 (MQ=255)
                         ttCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                                                                              <  2:15560/150‑1 (MQ=255)
                         ttCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTAATCATAATAATGATCTCACGGTAAGGGGACAGGTAACTCCGCGAAGGCGGAATAACCTTTTTATTATTTCAgctcgc                                                                                                                              >  2:339046/1‑150 (MQ=255)
                          tCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCAGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGcc                                                                                                                             >  1:129262/1‑150 (MQ=255)
                           cggcggCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCt                                                                                                                            <  2:711310/150‑1 (MQ=255)
                             gcggcAGAAAAAGCGCTGGTTGACAACATTAAGACCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTgg                                                                                                                          <  2:1662994/150‑1 (MQ=255)
                              cggcAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGt                                                                                                                         >  1:1457687/1‑150 (MQ=255)
                               ggcATCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                                                                        <  2:1326595/150‑1 (MQ=255)
                               ggcAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTGCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                                                                        <  1:1621826/150‑1 (MQ=255)
                                 cagcaAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgag                                                                                                                      >  2:555152/1‑150 (MQ=255)
                                    caAAAGCGCTGGTTGACAACCTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                                                                                   <  1:817917/150‑1 (MQ=255)
                                    caAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                                                                                   <  2:1218196/150‑1 (MQ=255)
                                     aaaaGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaaga                                                                                                                  >  2:498435/1‑150 (MQ=255)
                                      aaaGAGCTGGTTGACAACATTAAGCCCACGAGTGTGCAGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                                                                                 <  2:693785/150‑1 (MQ=255)
                                        aGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAAt                                                                                                               >  1:1560178/1‑150 (MQ=255)
                                          cgcTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                                                                             <  1:324188/150‑1 (MQ=255)
                                          cgcTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                                                                             <  1:862875/150‑1 (MQ=255)
                                            cTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTGAGATATTTATTCATAATAATGTTCTCATGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaac                                                                                                           <  2:1352632/150‑1 (MQ=255)
                                              ggTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                                                                                >  2:277650/1‑110 (MQ=39)
                                              ggTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                                                                                <  1:277650/111‑2 (MQ=39)
                                                ttGACAACATTAATCCCACGACTGTGCCGCAGAAATATATCAGAAATTTATTCATAATAATGTTCTCAAGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAAtt                                                                                                       <  2:261025/150‑1 (MQ=255)
                                                 tGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATATTAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                                                                      >  2:1649323/1‑150 (MQ=255)
                                                    caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                                                                   >  1:581446/1‑150 (MQ=255)
                                                    caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                                                                   >  2:66835/1‑150 (MQ=255)
                                                                cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                                                                                       <  1:1025822/150‑1 (MQ=255)
                                                                cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                                                                                       >  1:53477/1‑150 (MQ=255)
                                                                     gtgtGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatta                                                                                  <  1:459725/150‑1 (MQ=255)
                                                                        tgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGATTTTTTGCatattaata                                                                               <  1:555152/150‑1 (MQ=255)
                                                                          ccGCTGAAATATTTCAGATAATTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTATTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatttt                                                                             >  1:833286/1‑150 (MQ=255)
                                                                              tGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCt                                                                         <  2:1025954/150‑1 (MQ=255)
                                                                               gAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                                                                        >  1:233149/1‑150 (MQ=255)
                                                                               gAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGTATATTAATATTAGCTTg                                                                       >  1:262489/1‑150 (MQ=255)
                                                                                aaaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTg                                                                       <  1:498435/150‑1 (MQ=255)
                                                                                   tatTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                                                                    <  2:1457687/150‑1 (MQ=255)
                                                                                          tatatTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg                                                             <  1:1670636/149‑1 (MQ=255)
                                                                                          gATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTCCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAttgttg                                                             >  1:893979/1‑150 (MQ=255)
                                                                                          gATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg                                                             <  1:668996/150‑1 (MQ=255)
                                                                                           atatTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGa                                                            <  2:744762/150‑1 (MQ=255)
                                                                                              tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCt                                                         <  1:910275/150‑1 (MQ=255)
                                                                                               ttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTg                                                        >  2:1180857/1‑150 (MQ=255)
                                                                                                  ttCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc                                                     <  1:1216622/150‑1 (MQ=255)
                                                                                                     ataataATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGAc                                                  <  1:959670/150‑1 (MQ=255)
                                                                                                      taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACa                                                 >  2:1570858/1‑150 (MQ=255)
                                                                                                      taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACa                                                 >  2:1632357/1‑150 (MQ=255)
                                                                                                      taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACa                                                 <  2:301373/150‑1 (MQ=255)
                                                                                                       aataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACag                                                <  2:233149/150‑1 (MQ=255)
                                                                                                         taatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACagag                                              <  2:1624423/150‑1 (MQ=255)
                                                                                                           atGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGAc                                            >  1:403792/1‑150 (MQ=255)
                                                                                                              ttCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACaaa                                         >  1:638083/1‑150 (MQ=255)
                                                                                                               tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCata                                                                                     >  1:602206/1‑105 (MQ=255)
                                                                                                               tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCata                                                                                     <  2:602206/105‑1 (MQ=255)
                                                                                                               tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACaaaa                                        <  1:635856/150‑1 (MQ=255)
                                                                                                                 tcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACaaaaac                                      <  2:874043/150‑2 (MQ=255)
                                                                                                                       tAAGGGGACAGGTAACTCCGCGTTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCATCAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTc                                <  2:17564/150‑1 (MQ=255)
                                                                                                                       tAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAAAAATCAACAATTGAGCCGGTTTTTAGCAAATTAATATAAGAATGTTAAATATTAACATGGGCGACAGAGACAAAAAAAGCCTc                                >  2:190880/1‑150 (MQ=255)
                                                                                                                          ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAg                             >  1:1693224/1‑150 (MQ=255)
                                                                                                                           gggACCGGTAACTCCGCGATGGCGTAATTACCTTTATCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTATTGCATATAAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGc                            <  1:1150345/150‑1 (MQ=255)
                                                                                                                              aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCaaa                         >  1:752722/3‑150 (MQ=255)
                                                                                                                              aaaGGTAATTCCGCCATCGCGGAGTCACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCaaa                         >  1:1002394/3‑150 (MQ=255)
                                                                                                                               cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCTCGACTTGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTTCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                        <  1:731533/150‑1 (MQ=255)
                                                                                                                               cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCaaat                        >  2:1613603/1‑149 (MQ=255)
                                                                                                                               cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                        <  1:448954/150‑1 (MQ=255)
                                                                                                                               aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                        >  2:402327/2‑150 (MQ=255)
                                                                                                                                aGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagt                       >  1:1436190/1‑150 (MQ=255)
                                                                                                                                 ggTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagtt                      >  1:1079275/1‑150 (MQ=255)
                                                                                                                                 ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagtt                      >  2:402002/1‑150 (MQ=255)
                                                                                                                                    aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagttagt                   <  1:1623172/150‑1 (MQ=255)
                                                                                                                                      cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTcc                               <  2:844076/136‑1 (MQ=255)
                                                                                                                                      cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTcc                               >  1:844076/1‑136 (MQ=255)
                                                                                                                                      cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTg                 >  1:389014/1‑150 (MQ=255)
                                                                                                                                      cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTAAAGGTTGCGCTGGGCGACAGCGACAAAAAAAGCGTCCAGCAAAGTTAGTTg                 >  2:1270807/1‑150 (MQ=255)
                                                                                                                                        ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGACGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTa               >  2:897206/1‑150 (MQ=255)
                                                                                                                                           tgATGGCGGAATTACCTTTTTCTTATTTCAGCTCGCATGGTGATAATAAATCAACTATTGGTCCGGTTTTTTTCATATTAATATTAGCTTGTTTAATGTTGAGCTGGGCTACAGAGAGAAAAAAAGCGTCCAGCAAAGTTAGTTGTAatt            <  1:339046/149‑2 (MQ=255)
                                                                                                                                           cgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTTAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAata            <  1:1490662/150‑1 (MQ=255)
                                                                                                                                                gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCAGGGCGACAGAGACAAAAAAAGCTTCAAGCAAAGTTAGTTGTAATATTCtt       >  1:922497/1‑150 (MQ=255)
                                                                                                                                                 cgtaaTTAACTTTTTCTTATTTCAGCGTGCCTGGTGAGAAGATATCAACAATTTCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGATCTGTGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTg      <  1:94960/147‑1 (MQ=255)
                                                                                                                                                    aaTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCATCAATTTCGCAGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGGAAAGTTAGTTGTAATATTCTTGCCg   <  2:778041/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGA  >  NC_000913/4311940‑4312238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: