Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,529 C→G 7.7% Q248H (CAG→CAC) ‡ basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,5290CG 7.7% 415.6 / 21.2 130Q248H (CAG→CAC) ‡basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base C (61/59);  new base G (7/3);  total (68/62)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

Only 100 of 133 total aligned reads displayed.

TCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGG  >  NC_000913/4332380‑4332677
                                                                                                                                                     |                                                                                                                                                    
tcatcaATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCCTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAccc                                                                                                                                                      <  2:1404704/150‑1 (MQ=255)
 cttcaATACCTGGTCCTTCATCTTCAACTGCCATGACCGATCCGTCTACTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCTGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCt                                                                                                                                                     <  2:74268/148‑1 (MQ=255)
        aCCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGt                                                                                                                                              >  1:841030/1‑150 (MQ=255)
         ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                             <  1:1087825/150‑1 (MQ=255)
          cTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACTATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGa                                                                                                                                            >  1:825636/1‑150 (MQ=255)
            ggTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATg                                                                                                                                          <  2:649967/150‑1 (MQ=255)
              tCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTc                                                                                                                                        >  1:454301/1‑150 (MQ=255)
                 ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaacc                                                                                                                                     <  2:494590/150‑6 (MQ=255)
                 ttCATCTTCAACTGCCATGACCGCTCCGTCATCGTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                                                                                                       >  1:139569/1‑148 (MQ=255)
                  tcatcatcaACTGCCATGCCCGCTCCGTCAACTTCTTGAAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTATCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaaccc                                                                                                                                    <  1:1044842/150‑7 (MQ=255)
                   cATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggcgg                                                                                                                                   >  1:68982/1‑150 (MQ=255)
                    aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCggcggc                                                                                                                                  <  2:974063/150‑1 (MQ=255)
                    aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACAGTGATGTCggcggc                                                                                                                                  >  2:739023/1‑150 (MQ=255)
                     tCTTGAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGCTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGgcggcg                                                                                                                                 <  2:770317/150‑1 (MQ=255)
                     tCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGgcggcg                                                                                                                                 <  2:755430/150‑1 (MQ=255)
                       ttCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGct                                                                                                                               >  1:1012066/1‑150 (MQ=255)
                         cAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTCCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTCAACGGTGATGTCGGCGGCGctct                                                                                                                             >  2:1116387/1‑150 (MQ=255)
                          aaCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctc                                                                                                                            <  1:1501582/150‑1 (MQ=255)
                          aaCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctc                                                                                                                            >  2:134179/1‑150 (MQ=255)
                           aCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctct                                                                                                                           <  1:716828/150‑1 (MQ=255)
                            cTGCCTTGACCGCTCCGTCATCTTCTTCCAGCTTAATCATAATGTCGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                          <  1:1538467/150‑1 (MQ=255)
                              gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                        <  1:1415739/150‑1 (MQ=255)
                              gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                        >  1:428323/1‑150 (MQ=255)
                                    aCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCGCTCTCTGGCAATAGc                                                                                                                  >  2:390597/1‑150 (MQ=255)
                                     ccGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                 <  1:839751/150‑1 (MQ=255)
                                     ccGCTCCGTAATCTTCTTGCATCTAAATCATATTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACTGAACTTTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                 <  2:872610/150‑1 (MQ=255)
                                       gCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAgg                                                                                                               >  2:1628248/1‑150 (MQ=255)
                                          ccGTCATCTTCTTGCAGCTTAATCATAATGTTTCTGCCTTGCGGGCTGCAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                            <  2:127319/150‑1 (MQ=255)
                                          ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                            <  2:9807/150‑1 (MQ=255)
                                                tcttctTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGt                                                                                                      <  1:1628248/150‑1 (MQ=255)
                                                      tGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTGTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                >  2:1350440/1‑150 (MQ=255)
                                                       gCAGCTTAATCATAATGTTTCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGt                                                                                               <  2:1150085/150‑1 (MQ=255)
                                                        cAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTc                                                                                              >  1:350509/1‑150 (MQ=255)
                                                         aGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCg                                                                                             >  1:745546/1‑150 (MQ=255)
                                                         aGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGAAGGGTTTGCTGTCGCTGGtag                                                                                             >  2:1094986/1‑148 (MQ=255)
                                                          gCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCAGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                            >  2:1010452/1‑150 (MQ=255)
                                                             tAATCATAATGTTGCTGCCTTGCGGGCTGTATCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                         <  2:356317/150‑1 (MQ=255)
                                                             tAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                         <  2:500902/150‑1 (MQ=255)
                                                              aaTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAt                                                                                        >  2:762925/1‑150 (MQ=255)
                                                               aTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTAGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATg                                                                                       <  2:825636/150‑1 (MQ=255)
                                                               aTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGAGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTTAAGTCGGCGTCTCTCTCTGGCAATAGCAGGGTTTTCTTTCGCTGGTAGAGCATg                                                                                       >  1:503117/1‑150 (MQ=255)
                                                                tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                      <  2:1613690/150‑1 (MQ=255)
                                                                tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                      >  1:411672/1‑150 (MQ=255)
                                                                tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCAGAACGGTGATGTCGGCGGCGCTCTCTGGAAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAtcg                                                                                      >  2:1407735/1‑148 (MQ=255)
                                                                   tAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGCGCATGGTAc                                                                                   >  2:1496668/1‑150 (MQ=255)
                                                                   tAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTAc                                                                                   >  2:216126/1‑150 (MQ=255)
                                                                     aTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTg                                                                                 >  1:1559157/1‑150 (MQ=255)
                                                                     aTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTg                                                                                 <  1:211989/150‑1 (MQ=255)
                                                                        ttGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCTGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGt                                                                              <  1:1365636/150‑1 (MQ=255)
                                                                         tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCTGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGtt                                                                             >  2:1255840/1‑150 (MQ=255)
                                                                          gctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTc                                                                            >  1:332330/1‑150 (MQ=255)
                                                                              ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCa                                                                        <  2:411672/150‑1 (MQ=255)
                                                                              ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCa                                                                        <  1:1064/150‑1 (MQ=255)
                                                                               cTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCAt                                                                       >  2:962255/1‑150 (MQ=255)
                                                                                 tGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATAc                                                                     <  1:1320850/150‑1 (MQ=255)
                                                                                    gggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                  >  1:1022211/1‑150 (MQ=255)
                                                                                     ggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                 >  2:1186811/1‑150 (MQ=255)
                                                                                     ggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACAACCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                 >  1:188784/1‑150 (MQ=255)
                                                                                      gCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAggg                                                                <  1:428769/150‑1 (MQ=255)
                                                                                      acTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAggg                                                                >  2:525557/2‑150 (MQ=255)
                                                                                       cTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTTTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgggg                                                               >  1:1248070/1‑150 (MQ=255)
                                                                                          tAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGAAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCTCTCTCTGGCAATAGCAGGGTTTGCAGTCTCTGGTCGAGCATGGTAATGATTTCGTCATAAGAGGGaaga                                                            >  1:1043389/1‑150 (MQ=255)
                                                                                           aaCGATGGGCGTTTTCTA‑CAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAAt                                                          >  2:540868/1‑150 (MQ=255)
                                                                                               aTGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGAc                                                       >  2:676245/1‑150 (MQ=255)
                                                                                               aTGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGAc                                                       <  1:573345/150‑1 (MQ=255)
                                                                                                tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                      <  1:628868/150‑1 (MQ=255)
                                                                                                tGGGCGTTTTCTACCAGGTATCGCAATAACATCCGCAGCAGGGTTGCAGCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                      <  1:109160/150‑1 (MQ=255)
                                                                                                tGGGAGTTTTCTACCAGGTTTCTCAATAACATCCGCATAAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCTATGTTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                      <  1:1702278/150‑1 (MQ=255)
                                                                                                 gggCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACAt                                                     <  2:230738/150‑1 (MQ=255)
                                                                                                      ttttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTcc                                                <  1:990095/150‑1 (MQ=255)
                                                                                                        ttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                              <  2:1338892/150‑1 (MQ=255)
                                                                                                             ccAGGTTTCGTAATATCATCCGCAGGAGGTTTGCATCACCCTGTTCGGTGTTGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTTTCGCTGTTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGTATGACATCTTCCAGCAGtt                                         <  1:1407735/150‑1 (MQ=255)
                                                                                                               aGGTTTCGCCATAACATCCGCAGCAGGGTTGCAGCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                       <  1:62144/150‑1 (MQ=255)
                                                                                                                  tttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACa                                    <  1:762925/150‑1 (MQ=255)
                                                                                                                   ttCGCAATACCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACAt                                   <  1:200074/150‑1 (MQ=255)
                                                                                                                      gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                >  2:1334000/1‑150 (MQ=255)
                                                                                                                      gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                >  2:564541/1‑150 (MQ=255)
                                                                                                                       cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGGAATAGAAGGGTTTGCTGTCGATGGTCGAGCATGGTACTGAGTTCGTCATACGAGGCGAGAATGACATCTTCCCGCAGTATTACATGTTg                               >  2:837055/1‑150 (MQ=255)
                                                                                                                             cATCCGCAGCAGGGTTGCATCACCCTGAAAGCTGATGTCGGCGGCGCTCTCTGGCAATTGAAGGGTTTGATGTCGCTGGTCGAGCATGGTACTGAGTTCGAAATACGAGGGGAGAATGACATATTCCAGCAGTATTACATGTTGATAAtt                         >  1:1342311/1‑150 (MQ=255)
                                                                                                                              aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTa                        >  1:668328/1‑150 (MQ=255)
                                                                                                                              aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAGGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTa                        >  2:733681/1‑150 (MQ=255)
                                                                                                                              aTCCGCAGCAGGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAGTGACATCTTCCAGCAGTTTTACATGTTGATAATTa                        <  2:68982/150‑1 (MQ=255)
                                                                                                                                 cGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCg                     <  2:1612181/150‑1 (MQ=255)
                                                                                                                                  gcagcaGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCgg                    <  1:161763/150‑1 (MQ=255)
                                                                                                                                    agcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTTTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaa                  <  2:1248070/150‑1 (MQ=255)
                                                                                                                                     gcagGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGCATGACCTCTTCCAGCAGGTTTACATGGTGAGAGTTACCGgaag                 >  1:276473/1‑150 (MQ=255)
                                                                                                                                       ccgccgacATCACCGTTGAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa               >  1:1530416/8‑150 (MQ=255)
                                                                                                                                        gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa              >  1:1191102/1‑150 (MQ=255)
                                                                                                                                        gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTCGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa              >  2:1425623/1‑150 (MQ=255)
                                                                                                                                         ggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaaa             <  2:459111/150‑1 (MQ=255)
                                                                                                                                          gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt            <  1:1481288/150‑1 (MQ=255)
                                                                                                                                           cgacATCACCGTTCAGGGTGAAGTCGGCGGCGCTCTCTGGCAATAGCAGGGATAGCTGTCGCTCGTCGAGCATGGTACTGAGTTCGTCAAACGAGGAGAGAAAGACATCATCCAGCAGTTATAAATGTAGATAAATACCGGAAGAAAATg           >  1:78401/4‑150 (MQ=255)
                                                                                                                                            gacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          >  1:1416348/3‑150 (MQ=255)
                                                                                                                                            gacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa          >  1:1509194/3‑150 (MQ=255)
                                                                                                                                            agCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGACGAGCATCGTACTGAGTTCGTCATACCAGGGCAGAATGACATCTTCCAGAAGTTTTACATGTTGATAATTACCGGAACAAAATGa          >  2:235338/2‑150 (MQ=255)
                                                                                                                                               aTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTg       >  2:1577653/1‑150 (MQ=255)
                                                                                                                                                 cACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTc     >  2:1533433/1‑150 (MQ=255)
                                                                                                                                                  aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc    >  2:1121364/1‑150 (MQ=255)
                                                                                                                                                  aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAG‑TTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg   <  2:1012066/150‑1 (MQ=255)
                                                                                                                                                    ccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCgg  <  1:525557/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGG  >  NC_000913/4332380‑4332677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑