Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,534 C→G 7.4% V247L (GTT→CTT) ‡ basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,5340CG 7.4% 421.8 / 18.4 134V247L (GTT→CTT) ‡basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base C (61/62);  new base G (7/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

Only 100 of 136 total aligned reads displayed.

AATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCG  >  NC_000913/4332385‑4332683
                                                                                                                                                     |                                                                                                                                                     
aaTACCCGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGAGGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAAc                                                                                                                                                       <  2:1336976/150‑1 (MQ=255)
   aCCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGt                                                                                                                                                    >  1:841030/1‑150 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                   <  1:1087825/150‑1 (MQ=255)
         tCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTc                                                                                                                                              >  1:454301/1‑150 (MQ=255)
            ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                                                                                                             <  2:139569/148‑1 (MQ=255)
            ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaacc                                                                                                                                           <  2:494590/150‑6 (MQ=255)
            ttCATCTTCAACTGCCATGACCGCTCCGTCATCGTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                                                                                                             >  1:139569/1‑148 (MQ=255)
              cATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggcgg                                                                                                                                         >  1:68982/1‑150 (MQ=255)
               aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCggcggc                                                                                                                                        <  2:974063/150‑1 (MQ=255)
               aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACAGTGATGTCggcggc                                                                                                                                        >  2:739023/1‑150 (MQ=255)
                tCTTGAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGCTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGgcggcg                                                                                                                                       <  2:770317/150‑1 (MQ=255)
                  ttCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGct                                                                                                                                     >  1:1012066/1‑150 (MQ=255)
                    cAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTCCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTCAACGGTGATGTCGGCGGCGctct                                                                                                                                   >  2:1116387/1‑150 (MQ=255)
                     aaCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctc                                                                                                                                  <  1:1501582/150‑1 (MQ=255)
                      aCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctct                                                                                                                                 <  1:716828/150‑1 (MQ=255)
                       cTGCCTTGACCGCTCCGTCATCTTCTTCCAGCTTAATCATAATGTCGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                                <  1:1538467/150‑1 (MQ=255)
                         gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                              <  1:1415739/150‑1 (MQ=255)
                           cATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCaa                                                                                                                            <  2:778016/150‑1 (MQ=255)
                               aCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCGCTCTCTGGCAATAGc                                                                                                                        >  2:390597/1‑150 (MQ=255)
                                ccGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                       <  1:839751/150‑1 (MQ=255)
                                ccGCTCCGTAATCTTCTTGCATCTAAATCATATTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACTGAACTTTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                       <  2:872610/150‑1 (MQ=255)
                                  gCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAgg                                                                                                                     >  2:1578512/1‑150 (MQ=255)
                                     ccGTCATCTTCTTGCAGCTTAATCATAATGTTTCTGCCTTGCGGGCTGCAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                                  <  2:127319/150‑1 (MQ=255)
                                     ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                                  <  2:9807/150‑1 (MQ=255)
                                     ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                                  >  2:428769/1‑150 (MQ=255)
                                           tcttctTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGt                                                                                                            <  1:1628248/150‑1 (MQ=255)
                                                 tGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTGTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTgg                                                                                                      >  2:1350440/1‑150 (MQ=255)
                                                  gCAGCTTAATCATAATGTTTCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGt                                                                                                     <  2:1150085/150‑1 (MQ=255)
                                                   cAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTc                                                                                                    >  1:350509/1‑150 (MQ=255)
                                                    aGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGAAGGGTTTGCTGTCGCTGGtag                                                                                                   >  2:1094986/1‑148 (MQ=255)
                                                     gCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCAGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                                  >  2:1010452/1‑150 (MQ=255)
                                                      cTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAg                                                                                                 <  1:134179/150‑1 (MQ=255)
                                                       ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                                <  1:1052913/150‑1 (MQ=255)
                                                        tAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCa                                                                                               <  2:500902/150‑1 (MQ=255)
                                                         aaTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAt                                                                                              >  2:762925/1‑150 (MQ=255)
                                                           tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                            >  1:411672/1‑150 (MQ=255)
                                                           tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATgg                                                                                            <  2:1613690/150‑1 (MQ=255)
                                                           tCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCAGAACGGTGATGTCGGCGGCGCTCTCTGGAAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAtcg                                                                                            >  2:1407735/1‑148 (MQ=255)
                                                              tAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGCGCATGGTAc                                                                                         >  2:1496668/1‑150 (MQ=255)
                                                              tAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTAc                                                                                         >  2:216126/1‑150 (MQ=255)
                                                                aTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTg                                                                                       <  1:211989/150‑1 (MQ=255)
                                                                aTGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTg                                                                                       >  1:1559157/1‑150 (MQ=255)
                                                                   ttGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCTGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGt                                                                                    <  1:1365636/150‑1 (MQ=255)
                                                                    tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCTGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGtt                                                                                   >  2:1255840/1‑150 (MQ=255)
                                                                         ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCa                                                                              <  2:411672/150‑1 (MQ=255)
                                                                         ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCa                                                                              <  1:1064/150‑1 (MQ=255)
                                                                            tGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATAc                                                                           <  1:1320850/150‑1 (MQ=255)
                                                                               gggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                        >  1:1022211/1‑150 (MQ=255)
                                                                               gggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAg                                                                        >  1:235204/1‑150 (MQ=255)
                                                                                ggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                       >  2:1186811/1‑150 (MQ=255)
                                                                                ggCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACAACCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                       >  1:188784/1‑150 (MQ=255)
                                                                                 gCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAggg                                                                      <  1:428769/150‑1 (MQ=255)
                                                                                   tGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGga                                                                    >  2:573345/1‑150 (MQ=255)
                                                                                     tAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGAAGGGTTGCATCACCCTGAACGGTGATGTCGGAGGCTCTCTCTGGCAATAGCAGGGTTTGCAGTCTCTGGTCGAGCATGGTAATGATTTCGTCATAAGAGGGaaga                                                                  >  1:1043389/1‑150 (MQ=255)
                                                                                      aaCGATGGGCGTTTTCTA‑CAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAAt                                                                >  2:540868/1‑150 (MQ=255)
                                                                                        cGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCTATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                               <  1:1116387/150‑1 (MQ=255)
                                                                                         gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTATGGCAATAGCAGGGTTTGCTGTCGATGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGa                                                              >  1:787269/1‑150 (MQ=255)
                                                                                          aTGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGAc                                                             >  2:676245/1‑150 (MQ=255)
                                                                                          aTGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACAGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGGCATACGAGGGGAGAATGAc                                                             <  1:739023/150‑1 (MQ=255)
                                                                                           tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                            <  1:628868/150‑1 (MQ=255)
                                                                                           tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                            <  2:332330/150‑1 (MQ=255)
                                                                                             ggCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATc                                                          <  1:428854/150‑1 (MQ=255)
                                                                                                 ttttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTcc                                                      <  1:990095/150‑1 (MQ=255)
                                                                                                  tttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCca                                                     <  1:170825/150‑1 (MQ=255)
                                                                                                   ttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                                    <  2:745546/150‑1 (MQ=255)
                                                                                                   ttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                                    <  2:1338892/150‑1 (MQ=255)
                                                                                                     cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                                  >  2:189444/1‑150 (MQ=255)
                                                                                                        ccAGGTTTCGTAATATCATCCGCAGGAGGTTTGCATCACCCTGTTCGGTGTTGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTTTCGCTGTTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGTATGACATCTTCCAGCAGtt                                               <  1:1407735/150‑1 (MQ=255)
                                                                                                          aGGTTTCGCCATAACATCCGCAGCAGGGTTGCAGCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                             <  1:62144/150‑1 (MQ=255)
                                                                                                             tttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACa                                          <  1:762925/150‑1 (MQ=255)
                                                                                                                cGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGt                                       >  1:374242/1‑150 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                      >  2:564541/1‑150 (MQ=255)
                                                                                                                  cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGGAATAGAAGGGTTTGCTGTCGATGGTCGAGCATGGTACTGAGTTCGTCATACGAGGCGAGAATGACATCTTCCCGCAGTATTACATGTTg                                     >  2:837055/1‑150 (MQ=255)
                                                                                                                  cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                     >  2:957139/1‑150 (MQ=255)
                                                                                                                         aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAGGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTa                              >  2:733681/1‑150 (MQ=255)
                                                                                                                         aTCCGCAGCAGGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAGTGACATCTTCCAGCAGTTTTACATGTTGATAATTa                              <  2:68982/150‑1 (MQ=255)
                                                                                                                            cGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCg                           <  2:1612181/150‑1 (MQ=255)
                                                                                                                            cGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCg                           <  2:503117/150‑1 (MQ=255)
                                                                                                                             gcagcaGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCgg                          <  1:161763/150‑1 (MQ=255)
                                                                                                                               agcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTTTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaa                        <  2:1248070/150‑1 (MQ=255)
                                                                                                                                gcagGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGCATGACCTCTTCCAGCAGGTTTACATGGTGAGAGTTACCGgaag                       >  1:276473/1‑150 (MQ=255)
                                                                                                                                  ccgccgacATCACCGTTGAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa                     >  1:1530416/8‑150 (MQ=255)
                                                                                                                                  agGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa                     >  2:1460183/1‑150 (MQ=255)
                                                                                                                                   gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa                    >  1:1191102/1‑150 (MQ=255)
                                                                                                                                     gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt                  <  1:1481288/150‑1 (MQ=255)
                                                                                                                                      cgacATCACCGTTCAGGGTGAAGTCGGCGGCGCTCTCTGGCAATAGCAGGGATAGCTGTCGCTCGTCGAGCATGGTACTGAGTTCGTCAAACGAGGAGAGAAAGACATCATCCAGCAGTTATAAATGTAGATAAATACCGGAAGAAAATg                 >  1:78401/4‑150 (MQ=255)
                                                                                                                                       gacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa                >  1:1509194/3‑150 (MQ=255)
                                                                                                                                       gacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa                >  1:1416348/3‑150 (MQ=255)
                                                                                                                                       agCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGACGAGCATCGTACTGAGTTCGTCATACCAGGGCAGAATGACATCTTCCAGAAGTTTTACATGTTGATAATTACCGGAACAAAATGa                >  2:235338/2‑150 (MQ=255)
                                                                                                                                        gCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGAc               >  1:64591/1‑150 (MQ=255)
                                                                                                                                            cACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTc           >  2:1533433/1‑150 (MQ=255)
                                                                                                                                             aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc          >  2:1121364/1‑150 (MQ=255)
                                                                                                                                             aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAG‑TTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg         <  2:1012066/150‑1 (MQ=255)
                                                                                                                                               ccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCgg        <  1:525557/150‑1 (MQ=255)
                                                                                                                                               ccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCgg        <  1:540868/150‑1 (MQ=255)
                                                                                                                                                 tGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCa      <  2:350509/150‑1 (MQ=255)
                                                                                                                                                 tGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCa      <  1:189444/150‑1 (MQ=255)
                                                                                                                                                 tGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCa      >  2:1264193/1‑150 (MQ=255)
                                                                                                                                                   aaCGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAcg    <  1:1334000/150‑1 (MQ=255)
                                                                                                                                                     cGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAcgcg  >  1:1130531/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCG  >  NC_000913/4332385‑4332683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑