Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,132 T→G 7.0% A75A (GCA→GCC) ‡ cutA ← divalent‑cation tolerance protein, copper sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,1320TG 7.0% 362.3 / 13.6 124A75A (GCA→GCC) ‡cutAdivalent‑cation tolerance protein, copper sensitivity
Reads supporting (aligned to +/- strand):  ref base T (60/54);  new base G (4/5);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 124 total aligned reads displayed.

CCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGC  >  NC_000913/4364984‑4365275
                                                                                                                                                    |                                                                                                                                                
ccGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTg                                                                                                                                                 <  2:377665/150‑1 (MQ=255)
    cAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCctg                                                                                                                                             >  1:862049/1‑150 (MQ=255)
       aaaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCActgctg                                                                                                                                          <  1:604200/150‑1 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGTTGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         <  2:306415/150‑1 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         <  2:536645/150‑1 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         >  2:626173/1‑150 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         >  1:1611801/1‑150 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTGGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         <  2:448641/150‑1 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGAGGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                         <  2:1197436/150‑1 (MQ=255)
          cGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTg                                                                                                                                       >  2:823899/1‑150 (MQ=255)
           ggAAGTGCTGCATAGTAGCAGGATCAGCGTAAAGATGCGTTTAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaat                                                                                                                                      <  2:829078/150‑4 (MQ=255)
           ggAAGTGCTGCAAAGTAGCAGGAGCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaat                                                                                                                                      <  1:938245/150‑4 (MQ=255)
            gAAGTGCTGCAAAGAAGCAGGATCAGCGTAAAGATGCGTTGAGCCAAGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTgag                                                                                                                                     <  1:750303/150‑1 (MQ=255)
             aaGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTgaga                                                                                                                                    <  1:1144489/150‑1 (MQ=255)
              aGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaatgcc                                                                                                                                   <  2:1555785/150‑7 (MQ=255)
               gTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATa                                                                                                                                  <  1:938821/150‑1 (MQ=255)
                 gctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACg                                                                                                                                >  1:1495225/1‑150 (MQ=255)
                  ctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACgg                                                                                                                               >  1:856499/1‑150 (MQ=255)
                   tgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGt                                                                                                                              >  1:382127/1‑150 (MQ=255)
                    gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGGTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                             >  2:1001054/1‑150 (MQ=255)
                    gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGAGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                             >  1:215483/1‑150 (MQ=255)
                       aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtt                                                                                                                         <  1:88603/150‑1 (MQ=255)
                       aaGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGtt                                                                                                                         <  1:667080/150‑1 (MQ=255)
                            gCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaa                                                                                                                    >  2:1058714/1‑150 (MQ=255)
                               ggATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAat                                                                                                                 <  2:542779/150‑1 (MQ=255)
                                   cAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAatcatc                                                                                                             <  2:1088336/150‑1 (MQ=255)
                                   cAGCCTAAACTTGCTTTGAGCCAGGAGAGGTAATCTGTGGCTCCGTGTGAAACAGGTAAAACCAGAAGGACCGGGGTTTGATATGGATGATGAGACTTCAGGCAATCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAatcatc                                                                                                             <  1:1525041/150‑1 (MQ=255)
                                    aGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCt                                                                                                            >  1:1318678/1‑150 (MQ=255)
                                     gCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTg                                                                                                           >  1:4229/1‑150 (MQ=255)
                                      cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTTGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                          <  2:923484/150‑1 (MQ=255)
                                      cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAATTCATCTGc                                                                                                          >  2:235200/1‑150 (MQ=255)
                                       gTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCa                                                                                                         >  1:916298/1‑150 (MQ=255)
                                         aaaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAAACAGAAGTTCAGGGGTTTGATATGGATGATGAGACTACAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTATAAAATCATCTGCACt                                                                                                       >  1:605696/1‑150 (MQ=255)
                                          aaGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACtt                                                                                                      <  1:1586801/150‑1 (MQ=255)
                                             aTGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGt                                                                                                   <  1:570848/150‑1 (MQ=255)
                                              tGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATATGCACTTCGtt                                                                                                  >  2:1631459/1‑149 (MQ=255)
                                               gCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTAt                                                                                                 <  1:1691598/150‑1 (MQ=255)
                                                cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCg                                                                                                    >  1:421997/1‑146 (MQ=255)
                                                cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCg                                                                                                    <  2:421997/146‑1 (MQ=255)
                                                cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTAtt                                                                                                >  1:342926/1‑150 (MQ=255)
                                                  ttGAGCCATGAGATGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGACACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCt                                                                                              <  2:165782/150‑1 (MQ=255)
                                                  ttGAGCCATGAGAGGTATTCTGTGTCTCAGTGTGTAACAGGTAAAACCAGAAGTTCGGGGGTTTGATATGGTTGATGAGACTTAAGGCATTCCAGCAGTCCCTGCTGGTGAGTTACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCt                                                                                              <  1:1292766/150‑1 (MQ=255)
                                                  ttGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCt                                                                                              <  2:1469899/150‑1 (MQ=255)
                                                    gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTg                                                                                            <  1:190973/150‑1 (MQ=255)
                                                    gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTg                                                                                            <  1:786331/150‑1 (MQ=255)
                                                     aGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGc                                                                                           >  1:612962/1‑150 (MQ=255)
                                                      gCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCt                                                                                          <  1:195438/150‑1 (MQ=255)
                                                         aTGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCa                                                                                       >  2:1045496/1‑150 (MQ=255)
                                                               ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                 >  1:1228999/1‑150 (MQ=255)
                                                                     cTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTccc                                                                           <  2:475683/150‑1 (MQ=255)
                                                                       gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTAAGGCATTCCAGCAGTGCCTGCTGGTGGGATACGGTAGTTTTTAAAATCATCTGAACTTCGTATTCTTGCTCCAGCTTACCTTCCCat                                                                         >  1:1270228/1‑149 (MQ=255)
                                                                         gtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTGTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTa                                                                       <  2:475276/150‑1 (MQ=255)
                                                                          tctcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTaa                                                                      >  2:3147/1‑150 (MQ=255)
                                                                           ctcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAAt                                                                     >  2:1274764/1‑150 (MQ=255)
                                                                           ctcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAAt                                                                     >  2:1278833/1‑150 (MQ=255)
                                                                            tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGt                                                                        >  2:1498519/1‑146 (MQ=255)
                                                                            tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTGTGATATGGAAGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCTTATTCTTGCTCGATATTACCTTCCCAGTAATa                                                                    >  2:106663/1‑150 (MQ=255)
                                                                               gtgtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagag                                                                 <  1:934889/150‑1 (MQ=255)
                                                                                  tgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagaga                                                              <  2:862049/150‑1 (MQ=255)
                                                                                  tgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagaga                                                              >  2:781221/1‑150 (MQ=255)
                                                                                  tgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagaga                                                              >  1:1508033/1‑150 (MQ=255)
                                                                                   gtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagagag                                                             >  1:1192921/1‑150 (MQ=255)
                                                                                    tAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAgg                                                            >  1:761550/1‑150 (MQ=255)
                                                                                    tAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAgg                                                            <  1:113465/150‑1 (MQ=255)
                                                                                            aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCg                                                    >  2:1398991/1‑150 (MQ=255)
                                                                                              aaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATACTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCggg                                                  >  2:1410374/1‑150 (MQ=255)
                                                                                               agcAGAAGTTCAGGGTTTTGATATGGATGATGAGACTTCAGTCCTTCCATCAGTTCCTGCTGGTGAGATACGTTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                 <  1:75682/148‑1 (MQ=255)
                                                                                               aCCAGACGTTCCGGGGTTTGAAATGGATGCTGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                 <  2:240264/150‑1 (MQ=255)
                                                                                               aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                 >  2:1229632/1‑150 (MQ=255)
                                                                                               aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                 >  2:472810/1‑150 (MQ=255)
                                                                                                ccAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGa                                                >  1:1568106/1‑150 (MQ=255)
                                                                                                ccAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAAGCATCTGCACTTAGTATTCTTGCTCCAGCTGACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGa                                                >  1:1166166/1‑150 (MQ=255)
                                                                                                 cAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGAt                                               <  2:1647977/150‑1 (MQ=255)
                                                                                                   gAAGTTCCGGTGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTTCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                             <  1:893314/150‑1 (MQ=255)
                                                                                                   gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACATTCCCAGTAATAGAGAGAGGTAGCGCCTGGGATCa                                             >  1:321917/1‑150 (MQ=255)
                                                                                                      gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                          >  1:310183/1‑150 (MQ=255)
                                                                                                      gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                          <  1:1275634/150‑1 (MQ=255)
                                                                                                       ttCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGt                                         <  1:565368/150‑1 (MQ=255)
                                                                                                         ccGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTcg                                       <  1:1161927/150‑1 (MQ=255)
                                                                                                          cGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTcgc                                      >  1:654185/1‑150 (MQ=255)
                                                                                                           ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTcgcg                                     >  2:181707/1‑150 (MQ=255)
                                                                                                           ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTcgcg                                     >  2:946720/1‑150 (MQ=255)
                                                                                                                ttGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgc                                >  2:1447758/1‑150 (MQ=255)
                                                                                                                 tGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgcc                               <  1:1062966/150‑1 (MQ=255)
                                                                                                                  gATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccg                              <  1:1058714/150‑1 (MQ=255)
                                                                                                                      tGGATGATGAGACTTCAGTCATTCGAGCAGAGCATGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCAAGCTTACCTTCCCTGTAATAGTGATATGTACATCCGTGTATCAATGTCGCGCAGGCCGCCAg                          <  2:255000/150‑1 (MQ=255)
                                                                                                                      tGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAg                          >  2:587372/1‑150 (MQ=255)
                                                                                                                          tgatgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGtttt                      >  2:1646714/1‑150 (MQ=255)
                                                                                                                           gatgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGttttt                     <  1:626173/150‑1 (MQ=255)
                                                                                                                                         cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg       >  1:928242/1‑150 (MQ=255)
                                                                                                                                         cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTACAGCTTACCTTCCCAGTAATAGAGAGATGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCAAGATTTTCCGCCAGAACTTTg       >  2:1043285/1‑150 (MQ=255)
                                                                                                                                          tcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg      >  1:1163159/4‑150 (MQ=255)
                                                                                                                                          aTTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg      <  2:382127/150‑1 (MQ=255)
                                                                                                                                          aTTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg      <  1:472810/150‑1 (MQ=255)
                                                                                                                                          aTTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg      >  2:951121/1‑150 (MQ=255)
                                                                                                                                          aTTCCAGCAGTGCCTGCTGTGAGATACGGTAGTTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCATGCCGCCAGTTTTTCCGCCAGCACTTTgt      >  2:826125/1‑149 (MQ=255)
                                                                                                                                             ccAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTagcgg   >  1:305698/1‑150 (MQ=255)
                                                                                                                                              caggagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGTATCTAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcggc  <  1:1029988/150‑1 (MQ=255)
                                                                                                                                              cagcagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcggc  >  1:1484788/1‑150 (MQ=255)
                                                                                                                                              cagcagTGCATGCTGGTGAGATACGGTAGTTTTTAAAATAATCTGCACTTCGTATTCTTGATCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCACATTTTCCGCCAGCACTTTggcggc  >  1:1358190/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGC  >  NC_000913/4364984‑4365275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: