Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 448,368 A→T 6.4% V98D (GTT→GAT) ‡ cyoC ← cytochrome o ubiquinol oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913448,3680AT 6.4% 363.0 / 10.8 134V98D (GTT→GAT) ‡cyoCcytochrome o ubiquinol oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base A (61/63);  new base T (5/4);  total (66/68)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01

Only 100 of 136 total aligned reads displayed.

CCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAACG  >  NC_000913/448221‑448517
                                                                                                                                                   |                                                                                                                                                     
ccGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAAcc                                                                                                                                                     >  1:13538/1‑150 (MQ=255)
 cgtgcgtgCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCATGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                                                    <  1:699785/150‑1 (MQ=255)
 cgtgcgtgCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCt                                                                                                                                                    >  1:1213185/1‑150 (MQ=255)
    gcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTAAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGTTATCTCCTGGc                                                                                                                                                 <  1:116803/150‑1 (MQ=255)
    gcgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCAGGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGc                                                                                                                                                 <  1:818465/150‑1 (MQ=255)
     cgtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCt                                                                                                                                                >  2:1233655/1‑150 (MQ=255)
      gtgcCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCAGATAAATCCGGCACCAAACAACCAGGTCAACTCCAGCCACGAGATAACCTCGCtt                                                                                                                                               >  1:767564/1‑150 (MQ=255)
         ccGACCAGCGCAAAGAACGCTGACATGACGCCGCTGCGATCCTGACCCCTGCCGTTAACAATAAGGTTATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCATCGCCAGCCAGGAGATAACCTGGCTTttg                                                                                                                                            <  1:1087238/150‑1 (MQ=255)
         ccGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGTTATCTCCTGGCtggcg                                                                                                                                            <  1:709905/150‑5 (MQ=255)
            aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgttg                                                                                                                                         <  2:928133/150‑1 (MQ=255)
            aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTttgttg                                                                                                                                         <  1:1378198/150‑1 (MQ=255)
            aCCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTCATGGAATTCATAGATATCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCATGAGATAACCTGGCTTttgttg                                                                                                                                         <  2:1040990/150‑1 (MQ=255)
                 cgcAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGttttt                                                                                                                                    <  1:796673/150‑1 (MQ=255)
                   cAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTa                                                                                                                                 >  2:1462503/1‑150 (MQ=255)
                   cAAAGAACGCGGACAGGAAGCCGCGGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGt                                                                                                                                  <  2:875980/150‑1 (MQ=255)
                    aaaGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTa                                                                                                                                 <  1:308092/150‑1 (MQ=255)
                    aaaGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTa                                                                                                                                 <  1:747975/150‑1 (MQ=255)
                     aaGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTAc                                                                                                                                <  1:1320117/150‑1 (MQ=255)
                      aGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACa                                                                                                                               <  2:1442446/150‑1 (MQ=255)
                        aaCGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCACCGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATg                                                                                                                             >  2:199806/1‑150 (MQ=255)
                         aCGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATgg                                                                                                                            <  2:981634/150‑1 (MQ=255)
                         aCGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATgg                                                                                                                            <  2:1535338/150‑1 (MQ=255)
                          cGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGc                                                                                                                           <  1:12155/150‑1 (MQ=255)
                           gCTGACAGGAAGCCGCTGCGTTCCGGACCCTTGCCGTTTACATTCAGGTGATGGAATTCATTGATTTCGATCCCGATAAATCCGGCACCAAAGAACGAGGTCAACGCCAGCCAGGAGATATCCTGGCTTTTGTTGTTTTTGTAGATGGCg                                                                                                                          <  2:767564/150‑1 (MQ=255)
                             tGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGAt                                                                                                                        >  1:724120/1‑150 (MQ=255)
                             tGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGAt                                                                                                                        >  1:450279/1‑150 (MQ=255)
                                cAGGAAGCCGCTGCGATCCGGACCCAAGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCTATAgc                                                                                                                     >  2:150165/1‑150 (MQ=255)
                                 aGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAgcc                                                                                                                    <  2:177702/150‑1 (MQ=255)
                                 aGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAgcc                                                                                                                    <  1:380243/150‑1 (MQ=255)
                                     aGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCa                                                                                                                >  2:590360/1‑150 (MQ=255)
                                          cTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCg                                                                                                           >  1:1024518/1‑150 (MQ=255)
                                             cGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAg                                                                                                        >  1:763083/1‑150 (MQ=255)
                                             cGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAg                                                                                                        >  2:1421694/1‑150 (MQ=255)
                                             cGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCAACCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAg                                                                                                        >  2:27033/1‑150 (MQ=255)
                                               aTCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGt                                                                                                      >  2:1377418/1‑150 (MQ=255)
                                                 ccGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGa                                                                                                    <  1:1629329/150‑1 (MQ=255)
                                                  cGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGAt                                                                                                   >  1:1019595/1‑150 (MQ=255)
                                                    gACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATgg                                                                                                 <  1:585265/150‑1 (MQ=255)
                                                    gACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATgg                                                                                                 <  2:1292699/150‑1 (MQ=255)
                                                                     aTCAGGTGATGGAGTTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAg                                                                                <  2:1663156/150‑1 (MQ=255)
                                                                     aTCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGATTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGATCAACAGCAAg                                                                                >  1:1440369/1‑150 (MQ=255)
                                                                        aGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGaaa                                                                             >  1:987120/1‑150 (MQ=255)
                                                                         ggTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAg                                                                            <  2:1000140/150‑1 (MQ=255)
                                                                          gTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGt                                                                           >  1:927991/1‑150 (MQ=255)
                                                                           tGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGtt                                                                          >  2:705957/1‑150 (MQ=255)
                                                                             aTGGAATTCTTAGAGTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTc                                                                        <  1:277706/150‑1 (MQ=255)
                                                                              tGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCt                                                                                             >  2:1022225/1‑128 (MQ=255)
                                                                              tGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCt                                                                                             <  1:1022225/128‑1 (MQ=255)
                                                                              tGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCa                                                                       >  2:1551415/1‑150 (MQ=255)
                                                                               ggAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGGCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCaa                                                                      <  2:289420/150‑1 (MQ=255)
                                                                                  aTTCATAGAATTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACATGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGACCAACAGCAAGAAAGTTTCAACCa                                                                   >  1:307781/1‑150 (MQ=255)
                                                                                     cATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaa                                                                >  1:1154381/1‑150 (MQ=255)
                                                                                      aTAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaac                                                               <  2:525938/150‑1 (MQ=255)
                                                                                       tAGATTTCCATCCCGATAAATCCGGCAACAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacg                                                              <  1:1439791/150‑1 (MQ=255)
                                                                                         gATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacgaa                                                            >  1:957165/1‑150 (MQ=255)
                                                                                         gATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTCGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacgaa                                                            >  2:278188/1‑150 (MQ=255)
                                                                                          aTTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAgaacgaac                                                           >  1:452354/1‑150 (MQ=255)
                                                                                              ccATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCACCCAGACCGAACGGCa                                                       >  2:1573094/1‑150 (MQ=255)
                                                                                                  cccGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTc                                                   <  1:1233655/150‑1 (MQ=255)
                                                                                                   ccGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCg                                                  >  1:181795/1‑150 (MQ=255)
                                                                                                   ccGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCg                                                  >  2:1438495/1‑150 (MQ=255)
                                                                                                    ctaaaaaaCCGCCACCAAACACCCAGGTCAACGCCAGCCAGGACATACCCTGGCTTTTGTTTTTTTTGTCCATGGCGAACGCCGCCATGCCTTAGGTGATGGCGCTCAACAACACCAAGAAAGTTCCACCCACAACTAACGGCAGTTCGa                                                 <  2:1382019/146‑1 (MQ=255)
                                                                                                     gATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaa                                                >  1:1656117/1‑150 (MQ=255)
                                                                                                      aTAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGaaa                                               <  2:1593593/150‑1 (MQ=255)
                                                                                                        aaaTCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACTGCCGTTCgttctg                                             <  1:606360/150‑6 (MQ=255)
                                                                                                         aaTCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATg                                            <  2:763083/150‑1 (MQ=255)
                                                                                                           tCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTTCATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTc                                          >  2:415938/1‑150 (MQ=255)
                                                                                                            ccGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTcc                                         <  1:590360/150‑1 (MQ=255)
                                                                                                                cACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                     <  2:987120/150‑1 (MQ=255)
                                                                                                                cACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                     <  2:724120/150‑1 (MQ=255)
                                                                                                                cACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                     >  2:536076/1‑150 (MQ=255)
                                                                                                                cACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAc                                     <  2:450279/150‑1 (MQ=255)
                                                                                                                 aCCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTAcc                                    <  2:35947/150‑1 (MQ=255)
                                                                                                                   cAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTg                                  >  2:1406885/1‑150 (MQ=255)
                                                                                                                    aaaCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGt                                 <  2:1464435/150‑1 (MQ=255)
                                                                                                                    aaaCAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGCAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGt                                 >  2:1108964/1‑150 (MQ=255)
                                                                                                                           cAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgccg                          >  2:413319/1‑150 (MQ=255)
                                                                                                                           cAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGATCGAAAATGTCCTTACCTCTCGGgccg                          >  2:1044389/1‑150 (MQ=255)
                                                                                                                             ggTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACACCAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgcagcc                        >  2:1088559/1‑150 (MQ=255)
                                                                                                                             ggTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgccgcc                        >  2:957426/1‑150 (MQ=255)
                                                                                                                             ggTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGgccgcc                        >  2:718124/1‑150 (MQ=255)
                                                                                                                               tCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTg                      >  1:1537674/1‑150 (MQ=255)
                                                                                                                                 aaCGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCg                    >  1:700681/1‑150 (MQ=255)
                                                                                                                                   caaaagccagGTTATCTCCTGGCTGGCGTTGTTTTTGTACATGGCGATAGACGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGt                  >  2:498658/5‑150 (MQ=255)
                                                                                                                                     aaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGc                >  1:539877/3‑150 (MQ=255)
                                                                                                                                     aaagccagGTTATCTCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGcc               >  1:1569874/3‑150 (MQ=255)
                                                                                                                                      cagccagGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGcc               <  2:1213185/150‑1 (MQ=255)
                                                                                                                                       agccagGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCg              >  1:1586110/1‑150 (MQ=255)
                                                                                                                                       agccagGAGATAACCTGGCTTTTGTGGTGTTTGTACATGGCTATAGCCGCCATGCCGTAGGTGAGGGAGCTGAACAACAGCAAGAAAGTATCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCg              <  2:445115/150‑1 (MQ=255)
                                                                                                                                        gccagGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGt             >  2:1185733/1‑150 (MQ=255)
                                                                                                                                          cagGAGATAACCTGGCTTTTGTGGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTc           <  1:173555/150‑1 (MQ=255)
                                                                                                                                             gctaTAACCTGGCTTTTGTTGTTTTTGTACATGGAGAGAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGACCGGCAGATCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCAcc        <  1:257194/147‑1 (MQ=255)
                                                                                                                                             gagaTAACCTGGCTTTTGTTGTTATTGTACAGGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTGCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCAcc        <  1:840952/150‑1 (MQ=255)
                                                                                                                                              agaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCa       >  1:1476513/1‑150 (MQ=255)
                                                                                                                                              agaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCa       >  1:1670234/1‑150 (MQ=255)
                                                                                                                                               gaTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAg      <  2:1154381/150‑1 (MQ=255)
                                                                                                                                                aTAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGa     <  1:27033/150‑1 (MQ=255)
                                                                                                                                                 tAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGaa    >  1:1488889/1‑150 (MQ=255)
                                                                                                                                                  aaCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAAc   >  1:725428/1‑150 (MQ=255)
                                                                                                                                                   aCCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAACg  >  1:1488390/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCGTGCGTGCCGACCAGCGCAAAGAACGCTGACAGGAAGCCGCTGCGATCCGGACCCATGCCGTTAACAATCAGGTGATGGAATTCATAGATTTCCATCCCGATAAATCCGGCACCAAACAACCAGGTCAACGCCAGCCAGGAGATAACCTGGCTTTTGTTGTTTTTGTACATGGCGATAGCCGCCATGCCGTAGGTGATGGAGCTGAACAACAGCAAGAAAGTTTCAACCAGAACGAACGGCAGTTCGAAAATGTCCTTACCTGTCGGGCCGCCTGCGGTGCCGTTCACCAGAACG  >  NC_000913/448221‑448517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: