Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,385,359 T→A 5.8% intergenic (+18/‑34) yjeM → / → yjeN putative transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,3590TA 5.8% 397.5 / 8.7 137intergenic (+18/‑34)yjeM/yjeNputative transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (63/66);  new base A (3/5);  total (66/71)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 139 total aligned reads displayed.

TTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTG  >  NC_000913/4385211‑4385508
                                                                                                                                                    |                                                                                                                                                     
ttGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTa                                                                                                                                                      <  1:1502312/150‑1 (MQ=255)
    aGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTATCGATGGCGATTTACAAGAACTATTGCAGCAGAGTGGCAAAATATCCGCAGTGGGCGGTGGAATAACCAGATGCCCTATCGTATACCta                                                                                                                                                  >  2:1656138/1‑149 (MQ=255)
      ccGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGAc                                                                                                                                                <  1:1604945/150‑1 (MQ=255)
        ggAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACAAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACGTGACGa                                                                                                                                              >  1:1253847/1‑150 (MQ=255)
          agaCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATa                                                                                                                                            <  2:907950/150‑1 (MQ=255)
          agaCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATa                                                                                                                                            <  2:1049901/150‑1 (MQ=255)
            aCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                                          <  1:650689/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAggg                                                                                                                                         <  2:1574081/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAggg                                                                                                                                         <  1:1339860/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  2:2404/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  1:721413/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  1:1670456/150‑1 (MQ=255)
             cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAAGAACCCGCTGCCCTATCGTCTCCCTGACGATAgcg                                                                                                                                         >  2:1112757/1‑148 (MQ=255)
             cTGGGACAGCACATCGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCCGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  2:1070958/150‑1 (MQ=255)
             atGGGAAATCACATTGTTTATGCTTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTAACAGAAATTTTTATGCAGCGTGGCATAAAATCCGCAGTTTGCGGTGGAATAACCCGATGCCCTATCGTCATGTAGACGATAggg                                                                                                                                         <  2:784822/149‑1 (MQ=255)
                ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGt                                                                                                                                      >  1:628308/1‑150 (MQ=255)
                ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTAACAGAACTATTGCTGCAGAGTGGCATAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGTAAGGGCGt                                                                                                                                      >  1:859895/1‑150 (MQ=255)
                  aCAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTaa                                                                                                                                    <  2:1494895/150‑1 (MQ=255)
                     gCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGa                                                                                                                                 >  1:1720578/1‑150 (MQ=255)
                      cgcaTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTCTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTTAAGAc                                                                                                                                >  1:1044670/3‑150 (MQ=255)
                        caTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAg                                                                                                                              >  1:1095358/1‑150 (MQ=255)
                         aTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATTGCGATTTAACAGAACTATTGCAGCAGAGTGGCAATAAATCCGGAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAgg                                                                                                                             >  1:225293/1‑150 (MQ=255)
                          ttGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGa                                                                                                                            <  1:1571120/150‑1 (MQ=255)
                            gtgGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGaaa                                                                                                                          >  2:969191/1‑150 (MQ=255)
                              ggATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAgg                                                                                                                             <  2:1368807/145‑1 (MQ=255)
                              ggATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAgg                                                                                                                             >  1:1368807/1‑145 (MQ=255)
                                 tgatTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCCATGCCCTATCGACTACCTCACGATAGGGCGTAAAGACAGGAAATGTTa                                                                                                                     >  2:493622/1‑150 (MQ=255)
                                  gatTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGCTAGGGCGTAAAGACAGGAAATGTTAt                                                                                                                    <  2:1147600/150‑1 (MQ=255)
                                   atTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCTGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGTAATGTTATg                                                                                                                   <  1:266818/150‑1 (MQ=255)
                                    ttGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATgg                                                                                                                  <  1:460718/150‑1 (MQ=255)
                                      ggcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCAGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGAt                                                                                                                >  1:894517/1‑150 (MQ=255)
                                       gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               >  1:1112212/1‑150 (MQ=255)
                                       gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATCGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               <  2:127785/150‑1 (MQ=255)
                                        cggcCCTGTCTTCTTCTCGCGGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGa                                                                                                              <  1:1373783/150‑1 (MQ=255)
                                         ggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGCCCGGAAATGTTATGGATGac                                                                                                             >  2:1014998/1‑150 (MQ=255)
                                           cccTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                                           <  2:830965/150‑1 (MQ=255)
                                            ccTCTCTTCTTCTCGCTGTTAGCGATGGCGAATTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGCAATAACCCGCTGCCCTATCGTCTACCTGACGATACGGCGTAAAGACACGAAAAGATAAGGATGACAcc                                                                                                          >  1:1099606/1‑150 (MQ=255)
                                              gggcttcttctCGCTGTGAGCGTTGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAg                                                                                                        <  1:1251185/147‑1 (MQ=255)
                                               gTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGc                                                                                                       >  1:967492/1‑150 (MQ=255)
                                                 attcttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCg                                                                                                     <  2:621522/149‑1 (MQ=255)
                                                      tctcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGAcc                                                                                                <  1:821027/150‑1 (MQ=255)
                                                      tctcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGAcc                                                                                                <  2:1720578/150‑1 (MQ=255)
                                                        tcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCg                                                                                              >  2:439493/1‑150 (MQ=255)
                                                           cTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCa                                                                                           >  1:216501/1‑150 (MQ=255)
                                                            tGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCaa                                                                                          <  1:1001169/150‑1 (MQ=255)
                                                              ttAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATACCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATa                                                                                        <  2:302616/150‑1 (MQ=255)
                                                                aGCGATGGCGATTTACCAGAACAATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAc                                                                                      <  1:695366/150‑1 (MQ=255)
                                                                 gCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                                     <  1:49838/150‑1 (MQ=255)
                                                                 gCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGGGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                                     <  1:1546819/150‑1 (MQ=255)
                                                                 ccGATGGCGATTTACCAGAACTATAGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                                     <  2:32961/149‑1 (MQ=255)
                                                                    aTGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGaa                                                                                  >  1:510062/1‑150 (MQ=255)
                                                                     tGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAg                                                                                 <  1:971447/150‑1 (MQ=255)
                                                                        cGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                                              <  2:1601246/150‑1 (MQ=255)
                                                                        cGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                                              >  2:544494/1‑150 (MQ=255)
                                                                             tACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATc                                                                         >  2:160718/1‑150 (MQ=255)
                                                                              aCCAGAACTATTGCAGCATAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCa                                                                        <  1:477696/150‑1 (MQ=255)
                                                                              aCCAGAACTATTGCAGCAGAGTGGAAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGAAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATACCCGAAGATGAAATCa                                                                        >  2:1374284/1‑150 (MQ=255)
                                                                                cAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACTCCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAgg                                                                      >  1:382636/1‑150 (MQ=255)
                                                                                    aCTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACCCCCGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGgcgc                                                                  >  2:1090611/1‑150 (MQ=255)
                                                                                       aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:573451/1‑150 (MQ=255)
                                                                                       aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:1426199/1‑150 (MQ=255)
                                                                                          gcagcaGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                                            >  1:1500545/1‑150 (MQ=255)
                                                                                          gcagcaGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                                            >  1:288146/1‑150 (MQ=255)
                                                                                          gcagcaGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                                            <  2:1287920/150‑1 (MQ=255)
                                                                                                  gtgtCAATAAATCCGCAGTGTGCGGTGTAATAACCCGATGCACTATCGTCTTCCTGACGATAGGGCGTATAGACAGGAAATGTTATGTATGACACCAGCCGGGACCCGGCAATAACCTAAGATGAAATCAGGGCGCTGCAATTTTCTGcc                                                    <  2:1099606/150‑1 (MQ=255)
                                                                                                  gTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGcc                                                    <  2:1099604/150‑1 (MQ=255)
                                                                                                    ggCAAAAAATCCGCAGTGGGCTGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  >  2:1683087/1‑150 (MQ=255)
                                                                                                    ggCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGCTTCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  >  2:134951/1‑150 (MQ=255)
                                                                                                    ggCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  <  1:1715811/150‑1 (MQ=255)
                                                                                                      cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGCCAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCATTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgggg                                                >  1:1153957/1‑150 (MQ=255)
                                                                                                      cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGAGACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGGAAATTTCTGCCgggg                                                >  2:1202964/1‑150 (MQ=255)
                                                                                                      cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgggg                                                >  1:281145/1‑150 (MQ=255)
                                                                                                      cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgggg                                                >  1:58691/1‑150 (MQ=255)
                                                                                                       aaaaaaTCCGGAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGTTAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATTACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGa                                               <  1:357007/150‑1 (MQ=255)
                                                                                                          aaaTCCGCAGTGGGCGGGGGAATAACCCGATGCCCTATCGGCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGt                                            <  1:789317/150‑1 (MQ=255)
                                                                                                              ccGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCg                                        <  2:704546/150‑1 (MQ=255)
                                                                                                                gCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGa                                      <  1:890750/150‑1 (MQ=255)
                                                                                                                 cAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGaa                                     <  2:1514590/150‑1 (MQ=255)
                                                                                                                       gCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATagag                               >  2:1593821/1‑150 (MQ=255)
                                                                                                                        cGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGc                              <  1:1488064/150‑1 (MQ=255)
                                                                                                                         ggTGGAATAACCCGATGCCATATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCa                             <  2:281145/150‑1 (MQ=255)
                                                                                                                          gTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAg                            <  2:894517/150‑1 (MQ=255)
                                                                                                                           tGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGa                           >  1:688828/1‑150 (MQ=255)
                                                                                                                            ggAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGAc                          >  1:92227/1‑150 (MQ=255)
                                                                                                                            ggAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGAc                          <  2:383216/150‑1 (MQ=255)
                                                                                                                                  aCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtct                    >  2:563708/1‑150 (MQ=255)
                                                                                                                                   cccGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtctc                   <  2:1426199/150‑1 (MQ=255)
                                                                                                                                     cGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtctctc                 <  1:160718/150‑1 (MQ=255)
                                                                                                                                       aTGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCtt               <  2:1112212/150‑1 (MQ=255)
                                                                                                                                        tGCCCTATGGTCTACCTGACTATAGGGCGTAAAGCCAGTAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGTTGAAATCAGGGGGGTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCttt              <  1:493622/150‑1 (MQ=255)
                                                                                                                                        cgCCCTATCGTCAGGTAGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCttt              >  1:750874/2‑150 (MQ=255)
                                                                                                                                        cgCCCTATCGTCAGGTAGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCttt              >  2:335225/2‑150 (MQ=255)
                                                                                                                                        cgCCCTATCGTCAGGTAGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCACGGCGGTGCAAATTTCTGCCGGGGATGAAGCGTAAATAGACCAGACGATTctctctat              >  1:160543/2‑148 (MQ=255)
                                                                                                                                         gCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCtttt             >  2:497323/1‑150 (MQ=255)
                                                                                                                                          cccTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGATATAGAGCAGACGATTCTCTCTTTTg            >  2:92069/1‑150 (MQ=255)
                                                                                                                                          cccTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTg            >  1:788231/1‑150 (MQ=255)
                                                                                                                                             tATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACAGGGCAATAACCGAAGATGAAATAAGGGCGCTGCAAATTTCTGCAGGGGATGTAGCGGAAATAGAGCAGAAGATTATCTCCTTAGTCg         >  1:1465436/1‑150 (MQ=255)
                                                                                                                                                   cTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAGTCTCTCTTTTGTCGAtgcct   >  2:343090/1‑150 (MQ=255)
                                                                                                                                                    tACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGAtgcctg  <  2:705077/150‑1 (MQ=255)
                                                                                                                                                    tACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGAtgcctg  <  2:943187/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTG  >  NC_000913/4385211‑4385508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: