Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,385,361 C→G 5.8% intergenic (+20/‑32) yjeM → / → yjeN putative transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,3610CG 5.8% 443.7 / 14.0 138intergenic (+20/‑32)yjeM/yjeNputative transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (62/67);  new base G (3/5);  total (66/72)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 138 total aligned reads displayed.

AAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTG  >  NC_000913/4385214‑4385508
                                                                                                                                                   |                                                                                                                                                   
 aGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTATCGATGGCGATTTACAAGAACTATTGCAGCAGAGTGGCAAAATATCCGCAGTGGGCGGTGGAATAACCAGATGCCCTATCGTATACCta                                                                                                                                                  >  2:1656138/1‑149 (MQ=255)
   ccGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGAc                                                                                                                                                <  1:1604945/150‑1 (MQ=255)
   ccGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGAc                                                                                                                                                <  2:1027708/150‑1 (MQ=255)
     ggAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACAAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACGTGACGa                                                                                                                                              >  1:1253847/1‑150 (MQ=255)
       agaCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATa                                                                                                                                            <  2:907950/150‑1 (MQ=255)
       agaCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATa                                                                                                                                            <  2:1049901/150‑1 (MQ=255)
         aCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                                          <  1:644418/150‑1 (MQ=255)
         aCTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                                          <  1:650689/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAggg                                                                                                                                         >  1:1601246/1‑150 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAggg                                                                                                                                         <  2:1574081/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAggg                                                                                                                                         <  1:1339860/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  2:2404/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  1:721413/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  1:1670456/150‑1 (MQ=255)
          cTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAAGAACCCGCTGCCCTATCGTCTCCCTGACGATAgcg                                                                                                                                         >  2:1112757/1‑148 (MQ=255)
          cTGGGACAGCACATCGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCCGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCAGGTAGACGATAggg                                                                                                                                         <  2:1070958/150‑1 (MQ=255)
          atGGGAAATCACATTGTTTATGCTTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTAACAGAAATTTTTATGCAGCGTGGCATAAAATCCGCAGTTTGCGGTGGAATAACCCGATGCCCTATCGTCATGTAGACGATAggg                                                                                                                                         <  2:784822/149‑1 (MQ=255)
             ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGt                                                                                                                                      >  1:628308/1‑150 (MQ=255)
             ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTAACGTCTACCTGTCGATAGGGCGt                                                                                                                                      >  2:110904/1‑150 (MQ=255)
             ggACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTAACAGAACTATTGCTGCAGAGTGGCATAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGTAAGGGCGt                                                                                                                                      >  1:859895/1‑150 (MQ=255)
                  gCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGa                                                                                                                                 >  1:1720578/1‑150 (MQ=255)
                   cgcaTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTCTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTTAAGAc                                                                                                                                >  1:1044670/3‑150 (MQ=255)
                     caTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAg                                                                                                                              >  1:1095358/1‑150 (MQ=255)
                      aTTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATTGCGATTTAACAGAACTATTGCAGCAGAGTGGCAATAAATCCGGAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAgg                                                                                                                             >  1:225293/1‑150 (MQ=255)
                       ttGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGa                                                                                                                            <  1:1571120/150‑1 (MQ=255)
                         gtgGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGaaa                                                                                                                          >  2:969191/1‑150 (MQ=255)
                           ggATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAgg                                                                                                                             >  1:1368807/1‑145 (MQ=255)
                            gatgatTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGt                                                                                                                       <  2:1095358/150‑1 (MQ=255)
                              tgatTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCCATGCCCTATCGACTACCTCACGATAGGGCGTAAAGACAGGAAATGTTa                                                                                                                     >  2:493622/1‑150 (MQ=255)
                                atTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCTGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGTAATGTTATg                                                                                                                   <  1:266818/150‑1 (MQ=255)
                                atTGGCGGCCCTGTCTTCATCTCGCTGGTAGAGATGGCGATTTACCAGAACTATTGCTGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATg                                                                                                                   <  2:281926/150‑1 (MQ=255)
                                 ttGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATgg                                                                                                                  <  1:460718/150‑1 (MQ=255)
                                  tGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTAACTGACGATAGGGCGTAAAGACAGGAAATGTTATGGa                                                                                                                 >  2:1309562/1‑150 (MQ=255)
                                   ggcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCAGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGAt                                                                                                                >  1:894517/1‑150 (MQ=255)
                                    gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               >  1:1112212/1‑150 (MQ=255)
                                    gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               <  1:1521474/150‑1 (MQ=255)
                                    gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               <  1:232755/150‑1 (MQ=255)
                                    gcggcCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATCGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATg                                                                                                               <  2:127785/150‑1 (MQ=255)
                                     cggcCCTGTCTTCTTCTCGCGGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGa                                                                                                              <  1:1373783/150‑1 (MQ=255)
                                        cccTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGacac                                                                                                           <  2:830965/150‑1 (MQ=255)
                                         ccTCTCTTCTTCTCGCTGTTAGCGATGGCGAATTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGCAATAACCCGCTGCCCTATCGTCTACCTGACGATACGGCGTAAAGACACGAAAAGATAAGGATGACAcc                                                                                                          >  1:1099606/1‑150 (MQ=255)
                                           gggcttcttctCGCTGTGAGCGTTGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAg                                                                                                        <  1:1251185/147‑1 (MQ=255)
                                            gTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGc                                                                                                       >  1:967492/1‑150 (MQ=255)
                                              cttcttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCg                                                                                                     <  2:522110/150‑1 (MQ=255)
                                              attcttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCg                                                                                                     <  2:621522/149‑1 (MQ=255)
                                               ttcttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCgg                                                                                                    <  1:824734/150‑1 (MQ=255)
                                                 cttctCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAATTGTTATGGATGACACCAGCCGGGa                                                                                                  >  1:764726/1‑150 (MQ=255)
                                                   tctcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGAcc                                                                                                <  2:1720578/150‑1 (MQ=255)
                                                   tctcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGAcc                                                                                                <  1:821027/150‑1 (MQ=255)
                                                     tcGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCg                                                                                              >  2:439493/1‑150 (MQ=255)
                                                         tGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCaa                                                                                          <  1:1001169/150‑1 (MQ=255)
                                                           ttAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATACCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATa                                                                                        <  2:302616/150‑1 (MQ=255)
                                                             aGCGATGGCGATTTACCAGAACAATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAc                                                                                      <  1:695366/150‑1 (MQ=255)
                                                              gCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                                     <  1:49838/150‑1 (MQ=255)
                                                              ccGATGGCGATTTACCAGAACTATAGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                                     <  2:32961/149‑1 (MQ=255)
                                                                gATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGa                                                                                   >  1:1614872/1‑150 (MQ=255)
                                                                  tGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAg                                                                                 <  1:971447/150‑1 (MQ=255)
                                                                     cGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                                              >  2:544494/1‑150 (MQ=255)
                                                                     cGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                                              <  2:1601246/150‑1 (MQ=255)
                                                                      gATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGa                                                                             <  1:969191/150‑1 (MQ=255)
                                                                      gATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGa                                                                             >  2:1616035/1‑150 (MQ=255)
                                                                       aTTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGaa                                                                            >  1:704546/1‑150 (MQ=255)
                                                                           aCCAGAACTATTGCAGCAGAGTGGAAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGAAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATACCCGAAGATGAAATCa                                                                        >  2:1374284/1‑150 (MQ=255)
                                                                             cAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACTCCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAgg                                                                      >  1:382636/1‑150 (MQ=255)
                                                                                   tATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTg                                                                >  1:42270/1‑150 (MQ=255)
                                                                                   tATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTg                                                                >  1:191136/1‑150 (MQ=255)
                                                                                    aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:1426199/1‑150 (MQ=255)
                                                                                    aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:566661/1‑150 (MQ=255)
                                                                                    aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:573451/1‑150 (MQ=255)
                                                                                    aTTGCAGCAGAGTGGCAAAAAATCCGCAGTGGCCGGTGGAAAAACCCGATGACCTATCTTCTACCAGACGATACTCCGTAAAGACAGGAAATGTTATGGATGACACCAGCCCGGACCCGGCAAAAACCGAAGATGAAATCAGGGCGCTGc                                                               >  1:890958/1‑150 (MQ=255)
                                                                                       gcagcaGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                                            >  1:288146/1‑150 (MQ=255)
                                                                                       gcagcaGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                                            >  1:1500545/1‑150 (MQ=255)
                                                                                               gTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGcc                                                    <  2:1099604/150‑1 (MQ=255)
                                                                                                 ggCAAAAAATCCGCAGTGGGCTGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  >  2:1683087/1‑150 (MQ=255)
                                                                                                 ggCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGCTTCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  >  2:134951/1‑150 (MQ=255)
                                                                                                 ggCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgg                                                  >  1:147338/1‑150 (MQ=255)
                                                                                                   cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGCCAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCATTAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCgggg                                                >  1:1153957/1‑150 (MQ=255)
                                                                                                   cAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGAGACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGGAAATTTCTGCCgggg                                                >  2:1202964/1‑150 (MQ=255)
                                                                                                           ccGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCg                                        <  2:704546/150‑1 (MQ=255)
                                                                                                            cGCAGTGGGCGGTGGAATAACCCGATGCCCTAACGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCgg                                       <  1:495818/150‑1 (MQ=255)
                                                                                                              cAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCTATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGaa                                     <  1:300457/150‑1 (MQ=255)
                                                                                                              cAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGaa                                     <  2:1514590/150‑1 (MQ=255)
                                                                                                                  gggCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGAGGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATag                                 <  2:1148910/150‑1 (MQ=255)
                                                                                                                    gCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATagag                               >  2:1593821/1‑150 (MQ=255)
                                                                                                                      ggTGGAATAACCCGATGCCCTATCGTCTACCTTACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCa                             <  2:1514046/150‑1 (MQ=255)
                                                                                                                       gTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAg                            <  2:894517/150‑1 (MQ=255)
                                                                                                                         ggAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGAc                          <  2:383216/150‑1 (MQ=255)
                                                                                                                               aCCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtct                    >  2:563708/1‑150 (MQ=255)
                                                                                                                                cccGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtctc                   <  2:1426199/150‑1 (MQ=255)
                                                                                                                                 ccGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtctct                  >  2:336028/1‑150 (MQ=255)
                                                                                                                                  cGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATtctctc                 <  1:160718/150‑1 (MQ=255)
                                                                                                                                    aTGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCtt               <  2:1112212/150‑1 (MQ=255)
                                                                                                                                     tGCCCTATGGTCTACCTGACTATAGGGCGTAAAGCCAGTAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGTTGAAATCAGGGGGGTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCttt              <  1:493622/150‑1 (MQ=255)
                                                                                                                                       cccTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGATATAGAGCAGACGATTCTCTCTTTTg            >  2:92069/1‑150 (MQ=255)
                                                                                                                                       cccTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTg            >  1:788231/1‑150 (MQ=255)
                                                                                                                                         cTATCGTCTACCTGACGATACGGCGTAAAGACAGGAAATGTTATGGAAGACACCAGCCGCGACCCGTCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGATAAGACGATTctctctatagta          >  2:1371301/1‑144 (MQ=255)
                                                                                                                                          tATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACAGGGCAATAACCGAAGATGAAATAAGGGCGCTGCAAATTTCTGCAGGGGATGTAGCGGAAATAGAGCAGAAGATTATCTCCTTAGTCg         >  1:1465436/1‑150 (MQ=255)
                                                                                                                                                cTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAGTCTCTCTTTTGTCGAtgcct   >  2:343090/1‑150 (MQ=255)
                                                                                                                                                 tACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGAtgcctg  <  2:705077/150‑1 (MQ=255)
                                                                                                                                                 tACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGAtgcctg  <  2:943187/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGATTCTCTCTTTTGTCGATGCCTG  >  NC_000913/4385214‑4385508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: