Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,392,231 T→A 5.8% intergenic (+82/‑129) orn → / → glyV oligoribonuclease/tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,2310TA 5.8% 348.2 / 4.7 116intergenic (+82/‑129)orn/glyVoligoribonuclease/tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base T (53/56);  new base A (3/4);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CGATGGATGATATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGT  >  NC_000913/4392082‑4392379
                                                                                                                                                     |                                                                                                                                                    
cGATGGATGATTTCCTTGACTCGGTGGCTTAGCTGGCTTAGTACCGCGATCATTTTATGAATCTGTAAAATTTTTCCTGGTCTCGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAAtt                                                                                                                                                      <  1:337289/150‑1 (MQ=255)
 gatggatgATATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAAttt                                                                                                                                                     >  1:732413/1‑150 (MQ=255)
 gatggatgATATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTAACGTAAGCGCCGGAATCCACTTTCGGCGCCGTGATTTTGACGCTAAATTGAACGTCTTGCTGAAAAttt                                                                                                                                                     >  2:1344084/1‑150 (MQ=255)
   tggatgATATCCGTGAATCGGGGGCGGAGCTGGCTTACTACCGCGAGGTTTTTATCAAGCTGTAAATTTTTTCCTTGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCa                                                                                                                                                   <  1:1649933/150‑1 (MQ=255)
     gatgatATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAACTGAACGTCTTGCTGATAATTTCAGc                                                                                                                                                 >  1:998742/1‑150 (MQ=255)
        gatATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACt                                                                                                                                              >  1:603577/1‑150 (MQ=255)
          tatCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTg                                                                                                                                            >  1:147080/1‑150 (MQ=255)
          tatCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTg                                                                                                                                            >  2:1511438/1‑150 (MQ=255)
            tCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGAAATTATCAGCaagacgt                                                                                                                                          <  1:786254/150‑7 (MQ=255)
             ccGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATGATTTCAGCACTTgaga                                                                                                                                         <  1:439081/150‑1 (MQ=255)
             ccGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGAAATTATCAGCaagacgtt                                                                                                                                         <  2:144282/150‑8 (MQ=255)
              cGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTTGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGAt                                                                                                                                        <  2:837756/150‑1 (MQ=255)
              cGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGAt                                                                                                                                        <  1:364611/150‑1 (MQ=255)
                   aTCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATaaaaa                                                                                                                                   <  1:1511438/150‑1 (MQ=255)
                    tCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAAc                                                                                                                                  <  1:1022755/150‑1 (MQ=255)
                     cGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACg                                                                                                                                 >  1:181864/1‑150 (MQ=255)
                     cGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACg                                                                                                                                 <  1:995408/150‑1 (MQ=255)
                          gCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCaaaa                                                                                                                            <  2:1466679/150‑1 (MQ=255)
                            ggAGCTTGATTTCTACCTCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAATCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTTATAATTTCAGCACTTGAGATAAAAACGCaaaaaa                                                                                                                          <  2:11770/150‑1 (MQ=255)
                                cTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACt                                                                                                                      <  2:1197377/150‑1 (MQ=255)
                                cTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGAAAAAAACGCAAAAAAAACt                                                                                                                      <  2:955547/150‑1 (MQ=255)
                                  ggtttACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGCCGCTAAATTGAACGTCTTGCTGATAAATTCAGCACTTGAGATAAAAACGCAAAAAAAACttt                                                                                                                    >  2:1256064/4‑150 (MQ=255)
                                      tactacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTg                                                                                                                <  1:409230/150‑1 (MQ=255)
                                       actacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTgg                                                                                                               <  2:1328458/150‑1 (MQ=255)
                                        ctacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAGTTTTTTTTgcg                                                                                                              <  2:741038/150‑3 (MQ=255)
                                        ctacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTggg                                                                                                              >  2:1647999/1‑150 (MQ=255)
                                        ctacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTggg                                                                                                              >  2:570926/1‑150 (MQ=255)
                                        ctacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTggg                                                                                                              >  2:961658/1‑150 (MQ=255)
                                        ctacCGCGAGCATTTAATCAAGCTGTTAAATTTTTCCTTGTCACGTAAGCGCCGCAATCCACTAGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCATCACTTGAGATAAAAACGCAAAAAAAACTATTTTggg                                                                                                              >  1:489829/1‑150 (MQ=255)
                                         tacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTgggg                                                                                                             <  2:811024/150‑1 (MQ=255)
                                         tacCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTgggg                                                                                                             <  2:1103411/150‑1 (MQ=255)
                                          acCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTggggg                                                                                                            >  1:874678/1‑150 (MQ=255)
                                             gcgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGtt                                                                                                         <  2:1215640/150‑1 (MQ=255)
                                             gcgAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGtt                                                                                                         >  1:318763/1‑150 (MQ=255)
                                                  cATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAAGCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCaga                                                                                                    <  2:824745/150‑1 (MQ=255)
                                                    ttttATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAgg                                                                                                  <  2:437563/150‑1 (MQ=255)
                                                    ttttATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAgg                                                                                                  <  2:118634/150‑1 (MQ=255)
                                                     tttATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAggg                                                                                                 >  1:1116338/1‑150 (MQ=255)
                                                     tttATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAggg                                                                                                 >  1:826012/1‑150 (MQ=255)
                                                       tATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGaa                                                                                               >  2:714679/1‑150 (MQ=255)
                                                         tCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAg                                                                                             <  2:603577/150‑1 (MQ=255)
                                                          cAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGa                                                                                            <  1:933717/150‑1 (MQ=255)
                                                               tGTATAATTTTTGCTTGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTtct                                                                                       <  2:1050411/150‑1 (MQ=255)
                                                               tGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTtct                                                                                       <  1:1025742/150‑1 (MQ=255)
                                                                 tAAAATTTTTCCTGGTCACGTAAGCGCCGGACTCCACATGCTGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCg                                                                                     <  1:1130896/150‑1 (MQ=255)
                                                                 tAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCg                                                                                     <  2:1227940/150‑1 (MQ=255)
                                                                   aaaTTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGAACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGta                                                                                   >  2:64537/1‑150 (MQ=255)
                                                                    aaTTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCAGGtat                                                                                  >  2:708525/1‑150 (MQ=255)
                                                                        tttCCTTGTCACGTAAGCGCCGTAATCGACTTGCTGCTGCGTGATTTTGACTCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGTTGTTGCAGAGGGTAAGATTTCTCGTATAATg                                                                              <  1:1344084/150‑1 (MQ=255)
                                                                        tttCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATg                                                                              <  2:732413/150‑1 (MQ=255)
                                                                         ttCCTGGTCCCGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATgc                                                                             >  1:275114/1‑150 (MQ=255)
                                                                         ttCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGTGGTTGCAGAGGGAAAGATTTCTCGTATAATgc                                                                             <  1:1407642/150‑1 (MQ=255)
                                                                           ccTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATgcgc                                                                           >  2:480652/1‑150 (MQ=255)
                                                                               gTCACGTAAGCGCCGGAATCCACTTGCGGTGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTcc                                                                       <  1:726054/150‑1 (MQ=255)
                                                                                 cACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCg                                                                     >  2:876245/1‑150 (MQ=255)
                                                                                     tAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAac                                                                 >  1:1640919/1‑150 (MQ=255)
                                                                                         cgcCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAacgacg                                                             >  1:1394890/1‑150 (MQ=255)
                                                                                          gcCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGc                                                            >  2:74989/1‑150 (MQ=255)
                                                                                           ccGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCa                                                           <  1:1272589/150‑1 (MQ=255)
                                                                                              gAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGaa                                                        >  1:1629216/1‑150 (MQ=255)
                                                                                               aaTCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGaaa                                                       >  1:600696/1‑150 (MQ=255)
                                                                                                    aCTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGa                                                  <  2:318763/150‑1 (MQ=255)
                                                                                                              gcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa                                        <  2:147080/150‑1 (MQ=255)
                                                                                                              gcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa                                        >  2:1040071/1‑150 (MQ=255)
                                                                                                              gcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa                                        >  2:1132926/1‑150 (MQ=255)
                                                                                                              gcgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaa                                        >  1:174770/1‑150 (MQ=255)
                                                                                                               cgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaaa                                       <  2:1640919/150‑1 (MQ=255)
                                                                                                               cgTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGaaa                                       >  2:360476/1‑150 (MQ=255)
                                                                                                                 tGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGc                                     >  2:1615315/1‑150 (MQ=255)
                                                                                                                 tGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGc                                     <  2:181864/150‑1 (MQ=255)
                                                                                                                 tGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGc                                     >  2:432201/1‑150 (MQ=255)
                                                                                                                   aTTTTGTCGCTAAATTGAACGTCTTTCTTATAATTTGAGCACTTGAGATAAAAAAGCAAATAAAAGTTTTTTGGTGTTTTGCAGAGGGATAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCaa                                   <  2:489829/150‑1 (MQ=255)
                                                                                                                      ttGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAAt                                >  1:566088/1‑150 (MQ=255)
                                                                                                                          cGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTaat                            >  1:763386/1‑149 (MQ=255)
                                                                                                                          cGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAag                            >  1:20503/1‑150 (MQ=255)
                                                                                                                           gCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagt                           <  2:1405001/150‑1 (MQ=255)
                                                                                                                            cTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagta                          >  1:113480/1‑150 (MQ=255)
                                                                                                                              aaaTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagtagt                        >  2:825352/1‑150 (MQ=255)
                                                                                                                              aaaTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAagtagt                        >  1:345998/1‑150 (MQ=255)
                                                                                                                                aTTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAc                      >  1:1628162/1‑150 (MQ=255)
                                                                                                                                  tGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGAGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTAcgc                    >  2:847475/1‑150 (MQ=255)
                                                                                                                                     aCGTCTTGCTGATAATATCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCggg                 <  1:211460/150‑1 (MQ=255)
                                                                                                                                      cGTCTTGCTTATAATATCAGCACTTGAGATAAAAACGCAAAAAAAACTTATTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCCCATCACTAAACTACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGa                >  2:328139/1‑150 (MQ=255)
                                                                                                                                      cGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGa                >  1:1373741/1‑150 (MQ=255)
                                                                                                                                      cGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGa                <  2:566088/150‑1 (MQ=255)
                                                                                                                                       gTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGaa               >  2:1284797/1‑150 (MQ=255)
                                                                                                                                       caagtGCTGAAATTATCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGaa               >  1:383807/5‑150 (MQ=255)
                                                                                                                                          ttGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAg            <  1:74989/150‑1 (MQ=255)
                                                                                                                                           tGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGc           >  2:888729/1‑150 (MQ=255)
                                                                                                                                            gCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCt          >  1:157843/1‑150 (MQ=255)
                                                                                                                                            gCTGAAATTATCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCt          >  2:799015/1‑150 (MQ=255)
                                                                                                                                             cTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTc         <  2:1116338/150‑1 (MQ=255)
                                                                                                                                             cTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTc         <  2:1629216/150‑1 (MQ=255)
                                                                                                                                             cTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATAAAGTAGTACGCGGGAATAGCTc         <  2:826012/150‑1 (MQ=255)
                                                                                                                                              tGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCa        <  2:600696/150‑1 (MQ=255)
                                                                                                                                                 tAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGtt     <  1:818260/150‑1 (MQ=255)
                                                                                                                                                  aaTTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTg    <  1:1309626/150‑1 (MQ=255)
                                                                                                                                                  aaTTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTg    <  1:1422506/150‑1 (MQ=255)
                                                                                                                                                    tttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGt  >  1:888949/1‑150 (MQ=255)
                                                                                                                                                    tttCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGt  >  1:1344475/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGATGGATGATATCCGTGAATCGGTGGCGGAGCTGGCTTACTACCGCGAGCATTTTATCAAGCTGTAAAATTTTTCCTGGTCACGTAAGCGCCGGAATCCACTTGCGGCGGCGTGATTTTGACGCTAAATTGAACGTCTTGCTGATAATTTCAGCACTTGAGATAAAAACGCAAAAAAAACTTTTTTGGGGGGTTGCAGAGGGAAAGATTTCTCGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGT  >  NC_000913/4392082‑4392379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: