Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,455,418 T→G 5.1% K64N (AAA→AAC fbp ← fructose‑1,6‑bisphosphatase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,455,4180TG 5.1% 359.9 / 5.9 124K64N (AAA→AACfbpfructose‑1,6‑bisphosphatase I
Reads supporting (aligned to +/- strand):  ref base T (66/49);  new base G (5/2);  total (73/51)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAA  >  NC_000913/4455269‑4455565
                                                                                                                                                     |                                                                                                                                                   
ccATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGt                                                                                                                                                     >  1:984676/1‑150 (MQ=255)
   tCCAGGGTGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGATGACAATCTCATCTTCTTCTTCAGATGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTAAGTTTTTCATTAGCGAACAAGTCGAGTTTc                                                                                                                                                  <  2:151344/150‑1 (MQ=255)
   tCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAAAGCCCGCAACGATATCGCGTGCTTTCAATGCGGCTTTCAGTTTTTCATTAGCGAAACAGTCGAGttta                                                                                                                                                  >  2:1071916/1‑149 (MQ=255)
    ccAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTct                                                                                                                                                 >  1:1165004/1‑150 (MQ=255)
    ccAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTct                                                                                                                                                 >  1:897550/1‑150 (MQ=255)
     cAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTctg                                                                                                                                                <  1:1432664/150‑1 (MQ=255)
      aGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTctgc                                                                                                                                               <  1:780440/150‑1 (MQ=255)
      aGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTctgc                                                                                                                                               <  2:1165004/150‑1 (MQ=255)
        ggggTCCATCAGCACCACGTAATTTGCGTGTTCACAGCCTTCAAACACGACAATCTCATCTTCTACATCACAGGCAATGCCCCCAACGATATCGCGTGGTTTCACTGCGGCATTCAGATTTTCATTAGCCATCAACTCGAGATTcagctg                                                                                                                                             >  2:606761/1‑150 (MQ=255)
         gggTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGa                                                                                                                                            <  1:726232/150‑1 (MQ=255)
          ggTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGaa                                                                                                                                           >  1:1008326/1‑150 (MQ=255)
                 cAGCACCACGTTTTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGAGCTTTCAGTGCGGCTATCAGTTTTTAATTAGCGAACAAGTCGAGTTTCTACTCAACCTCGcc                                                                                                                                    >  1:350751/1‑150 (MQ=255)
                 cAGCACCACGTATTTTGCGAGTTCACAGCCTTCACAGACGACAAACTCCTCTTCTTCTTCAGCCGCAATGCCCGCCACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGcc                                                                                                                                    <  2:1584793/150‑1 (MQ=255)
                  aGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGCTATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGccc                                                                                                                                   <  2:604810/150‑1 (MQ=255)
                     accacGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGc                                                                                                                                <  2:461779/150‑1 (MQ=255)
                      ccacGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCa                                                                                                                               >  2:542591/1‑150 (MQ=255)
                        acGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACg                                                                                                                             >  2:1274558/1‑150 (MQ=255)
                         cGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGCACCTCGCCCTGCACGt                                                                                                                            >  1:1269671/1‑150 (MQ=255)
                         cGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGt                                                                                                                            <  2:643713/150‑1 (MQ=255)
                            aTTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTctgc                                                                                                                                               <  1:1353422/128‑1 (MQ=255)
                            aTTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTctgc                                                                                                                                               >  2:1353422/1‑128 (MQ=255)
                             ttttGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCTGAGGCTATGCCCGCATCGTTATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCTTTTGGGAACATGTCGTGTTTCTGCTGAACCTCGCCCTGCACGTtctc                                                                                                                        <  1:606761/150‑1 (MQ=255)
                              tttGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCa                                                                                                                       >  2:1618160/1‑150 (MQ=255)
                                tGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGc                                                                                                                     >  1:195158/1‑150 (MQ=255)
                                tGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGc                                                                                                                     >  1:639471/1‑150 (MQ=255)
                                tGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGc                                                                                                                     <  1:450882/150‑1 (MQ=255)
                                     gtTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCACTGCACGTTCTCAGCACCGc                                                                                                                >  1:124214/1‑150 (MQ=255)
                                      ttCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGCTATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGca                                                                                                               >  1:284979/1‑149 (MQ=255)
                                       tCACAGCCTTCAAAGACGACAATCTCATCATCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTg                                                                                                              >  2:115742/1‑150 (MQ=255)
                                        cacaGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTgg                                                                                                             >  1:476264/1‑150 (MQ=255)
                                         acaGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGc                                                                                                            <  2:476264/150‑1 (MQ=255)
                                         acaGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGc                                                                                                            >  2:1560229/1‑150 (MQ=255)
                                         acaGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGc                                                                                                            <  1:467497/150‑1 (MQ=255)
                                          caGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCa                                                                                                           >  1:194113/1‑150 (MQ=255)
                                            gCCTTCAAAGACGACATTCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCAcc                                                                                                         >  1:305817/1‑150 (MQ=255)
                                            gCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCAcc                                                                                                         >  1:924771/1‑150 (MQ=255)
                                             ccTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTATCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCAccc                                                                                                        >  1:961685/1‑150 (MQ=255)
                                              cTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCa                                                                                                       <  1:678523/150‑1 (MQ=255)
                                                 cAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACCAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGa                                                                                                    >  1:733984/1‑150 (MQ=255)
                                                 cAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGa                                                                                                    >  1:915702/1‑150 (MQ=255)
                                                 cAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGa                                                                                                    <  1:904124/150‑1 (MQ=255)
                                                      acgacAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCa                                                                                               >  1:949095/1‑150 (MQ=255)
                                                      acgacAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCa                                                                                               >  2:776588/1‑150 (MQ=255)
                                                       cgacAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCaa                                                                                              >  2:1252038/1‑150 (MQ=255)
                                                        gacCATCTCATCTTCTTCTTCAGAGCCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAc                                                                                             <  2:350751/150‑1 (MQ=255)
                                                        gacAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAc                                                                                             >  1:506065/1‑150 (MQ=255)
                                                        gacAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAc                                                                                             >  2:787374/1‑150 (MQ=255)
                                                         acAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAcc                                                                                            <  2:1554521/150‑1 (MQ=255)
                                                           aaTCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAg                                                                                          >  1:755546/1‑150 (MQ=255)
                                                            aTCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGt                                                                                         >  1:1635867/1‑150 (MQ=255)
                                                             tctcATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTc                                                                                        >  1:1659413/1‑150 (MQ=255)
                                                             tctcATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTc                                                                                        >  2:351422/1‑150 (MQ=255)
                                                             tctcATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTc                                                                                        >  2:331692/1‑150 (MQ=255)
                                                             tctcATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTc                                                                                        >  1:96023/1‑150 (MQ=255)
                                                                  tcttcttctTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAc                                                                                             >  2:931240/1‑140 (MQ=255)
                                                                   cttcttcttCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCtt                                                                                  <  1:1389495/150‑1 (MQ=255)
                                                                      cttcttcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTttgt                                                                               <  1:514360/150‑1 (MQ=255)
                                                                        tcttcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTttgttg                                                                             <  1:592259/150‑1 (MQ=255)
                                                                        tcttcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTttgttg                                                                             >  2:804297/1‑150 (MQ=255)
                                                                          ttcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGat                                                                           >  1:996660/1‑150 (MQ=255)
                                                                           tcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAcc                                                                                            >  1:1042898/1‑132 (MQ=255)
                                                                           tcAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAAcc                                                                                            <  2:1042898/132‑1 (MQ=255)
                                                                            cAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGatat                                                                         >  2:258880/1‑150 (MQ=255)
                                                                            cAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGatat                                                                         >  1:1622770/1‑150 (MQ=255)
                                                                                 gCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCGTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGCACCTCGCCCTGCACGTTCTCAGCACCGCTGGCCCCCAGGATATCACCCCGTCCTGCGTTGTAGAGATCGCGa                                                                    >  1:625672/1‑150 (MQ=255)
                                                                                  cAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATg                                                                  >  1:793520/1‑150 (MQ=255)
                                                                                         cGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAAt                                                            >  1:462002/1‑150 (MQ=255)
                                                                                             aCGATATCGCGTGCTTTCAGTGCGGCTATCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTg                                                        <  1:286561/150‑1 (MQ=255)
                                                                                               gATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGgc                                                      >  2:222091/1‑150 (MQ=255)
                                                                                                atatCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACACGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGgcg                                                     <  1:331692/150‑1 (MQ=255)
                                                                                                 tatCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGgcgc                                                    <  1:787374/150‑1 (MQ=255)
                                                                                                   tCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGccc                                                  <  1:1560229/150‑1 (MQ=255)
                                                                                                     gcgTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAg                                                <  2:897550/150‑1 (MQ=255)
                                                                                                        tGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGttt                                             <  1:1274558/150‑1 (MQ=255)
                                                                                                          cTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTAGGCGCCCAGTTTTa                                           >  1:1158019/1‑150 (MQ=255)
                                                                                                           tttGAGTGCGGCTTTCAGTTTTTCATTAGCGAAGAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTAt                                          <  2:244456/150‑1 (MQ=255)
                                                                                                               aGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGcc                                      <  2:501085/150‑1 (MQ=255)
                                                                                                                   cGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACa                                  <  1:1618160/150‑1 (MQ=255)
                                                                                                                     gCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGc                                <  1:1019351/150‑1 (MQ=255)
                                                                                                                       tttCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACACAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCaa                              >  1:382958/1‑150 (MQ=255)
                                                                                                                        ttCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGACGACAGCaaa                             >  2:213536/1‑150 (MQ=255)
                                                                                                                            gTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAg                         <  1:1188368/150‑1 (MQ=255)
                                                                                                                              ttttCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATTTCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTg                       <  2:1008326/150‑1 (MQ=255)
                                                                                                                              ttttCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTg                       <  2:1547396/150‑1 (MQ=255)
                                                                                                                                   aTTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTc                  >  2:1014402/1‑150 (MQ=255)
                                                                                                                                      aGCTAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCAcc               <  2:1489247/150‑1 (MQ=255)
                                                                                                                                      aGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTATGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTcccc               >  2:1153734/1‑150 (MQ=255)
                                                                                                                                         gAACAAGTCGAGGTTCTGCTGAACCTCGCCCTGCACGTTCTCATCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGt            <  1:115742/150‑1 (MQ=255)
                                                                                                                                          aaCAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGtt           >  1:1040757/1‑149 (MQ=255)
                                                                                                                                          aaCAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAAACTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTa           >  1:123178/1‑150 (MQ=255)
                                                                                                                                           aCAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAg          <  1:648857/150‑1 (MQ=255)
                                                                                                                                             agggcGAGGTTCAGCAGAAACTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACCGCAAAGCAGTCAGCTCCCCGGTAGCa        >  2:122608/5‑150 (MQ=255)
                                                                                                                                             aaGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTCTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCa        <  1:141318/150‑1 (MQ=255)
                                                                                                                                               ggcGAGGTTCAGCAGAAACTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGc         >  2:661552/3‑147 (MQ=255)
                                                                                                                                               gTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATg      <  2:984676/150‑1 (MQ=255)
                                                                                                                                                 cGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATgag    >  1:207835/1‑150 (MQ=255)
                                                                                                                                                 cGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATgag    >  2:938693/1‑150 (MQ=255)
                                                                                                                                                  gAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATTTAAACCAGTCCTGGTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATgaga   >  2:382980/1‑150 (MQ=255)
                                                                                                                                                  gAGGTTCAGCAGAAACTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATgaga   >  1:516346/1‑150 (MQ=255)
                                                                                                                                                   aGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGaa  <  1:258880/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAATGCCCGCAACGATATCGCGTGCTTTCAGTGCGGCTTTCAGTTTTTCATTAGCGAACAAGTCGAGTTTCTGCTGAACCTCGCCCTGCACGTTCTCAGCACCGCTGGCACCCAGGATATCAACCAGTCCTGCTTTGTTGATATCGCGATGGATAATCTTGGCGCCCAGTTTTATTGCCGACAGCAAAGCAGTGAGCTCACCGGTAGCATGAGAA  >  NC_000913/4455269‑4455565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: