Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,491,163 T→G 7.5% intergenic (‑24/+43) idnR ← / ← idnT transcriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,1630TG 7.5% 303.9 / 12.2 104intergenic (‑24/+43)idnR/idnTtranscriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (42/54);  new base G (4/4);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 114 total aligned reads displayed.

TTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAG  >  NC_000913/4491014‑4491307
                                                                                                                                                     |                                                                                                                                                
tttGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaat                                                                                                                                                  <  1:1115224/150‑1 (MQ=255)
     cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                             >  2:651247/1‑150 (MQ=255)
     cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                             <  1:941102/150‑1 (MQ=255)
     cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                             <  1:1312295/150‑1 (MQ=255)
      gATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaa                                                                                                                                            >  2:576662/1‑150 (MQ=255)
       aTGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaat                                                                                                                                           <  2:777507/150‑1 (MQ=255)
        tGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatc                                                                                                                                          >  1:395670/1‑150 (MQ=255)
            cTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTcc                                                                                                                                      <  1:1633531/150‑1 (MQ=255)
             tCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                     <  1:1074916/150‑1 (MQ=255)
             gcGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGAGGATATAACGACTCACGGTCATTTTTGATACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                     <  1:83760/149‑1 (MQ=255)
                ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGCCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                         >  2:814592/1‑143 (MQ=255)
                ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                         <  1:814592/143‑1 (MQ=255)
                 cTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                 <  1:1030544/150‑1 (MQ=255)
                 cTGTTTCCATTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACCTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                 <  2:109105/150‑1 (MQ=255)
                  tGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                <  2:369744/150‑2 (MQ=255)
                  ggTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                <  2:862642/149‑2 (MQ=255)
                   gTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGaaa                                                                                                                               >  2:1539903/1‑150 (MQ=255)
                      tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                            >  2:1418636/1‑150 (MQ=255)
                      tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                            >  2:1476854/1‑150 (MQ=255)
                      tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                            >  2:1483436/1‑150 (MQ=255)
                        cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                          <  2:942629/150‑1 (MQ=255)
                        cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                          <  2:920054/150‑1 (MQ=255)
                        cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGCTTCAATCTTCCCCCGAACCTAcc                                                                                                                          >  2:842868/1‑149 (MQ=255)
                        cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                          >  2:1679774/1‑150 (MQ=255)
                         ttttCCACCTTTTTCGTCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTATAGATATACTGTAACGAAATTCTGTGTTTGCGCATAACTTCTCCCAACGTCGGGGGTATCTTGAATCTTCCCCCGAAACTACAt                                                                                                                         <  2:1043929/150‑1 (MQ=255)
                          ttGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACAta                                                                                                                        >  2:462026/1‑149 (MQ=255)
                           tGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATca                                                                                                                       >  1:1621053/1‑150 (MQ=255)
                            gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                      >  1:64945/1‑150 (MQ=255)
                            gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGAGATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGGCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                      <  1:126153/150‑1 (MQ=255)
                            gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGGCATTTTTGTTACGCCAGCCAGCGTAGCGATAACCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCATCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                      <  2:431083/150‑1 (MQ=255)
                             ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                     >  2:720265/1‑150 (MQ=255)
                             ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                     <  1:630525/150‑1 (MQ=255)
                             ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCACCGAAACTACATcaca                                                                                                                     >  2:717126/1‑150 (MQ=255)
                              cACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACaa                                                                                                                    >  1:953156/1‑150 (MQ=255)
                                 cTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                 <  2:1662616/150‑1 (MQ=255)
                                 cTTTTTAGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                 >  1:898967/1‑150 (MQ=255)
                                     ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                             <  1:790315/150‑1 (MQ=255)
                                     ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                             <  1:1514924/150‑1 (MQ=255)
                                       cGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAtt                                                                                                           >  2:1445363/1‑150 (MQ=255)
                                        ggCGAGCGGATATAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAACCTACATCACAATAGTTTAtta                                                                                                          >  2:78743/1‑149 (MQ=255)
                                            aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                      <  1:1549497/150‑1 (MQ=255)
                                            aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                      <  1:428956/150‑1 (MQ=255)
                                             gCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCa                                                                                                     <  2:898967/150‑1 (MQ=255)
                                                gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                  >  2:1210167/1‑150 (MQ=255)
                                                gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                  <  1:909364/150‑1 (MQ=255)
                                                 atatAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                 <  2:457101/150‑1 (MQ=255)
                                                       cGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTC‑GGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                          >  1:1345694/1‑150 (MQ=255)
                                                        gACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                          <  1:1539903/150‑1 (MQ=255)
                                                        gACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                          >  2:784022/1‑150 (MQ=255)
                                                         aCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                         <  1:828087/150‑1 (MQ=255)
                                                           tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                       <  1:462026/150‑1 (MQ=255)
                                                           tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCACATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                       <  1:559558/150‑1 (MQ=255)
                                                               ggTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAAt                                                                                   >  2:1662601/1‑150 (MQ=255)
                                                                 tCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATgg                                                                                 <  1:1237550/150‑1 (MQ=255)
                                                                   aTTTTTGTTACGCCAGCCAGCGTATCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                               <  2:1497720/150‑1 (MQ=255)
                                                                   aTTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                               <  2:828855/150‑1 (MQ=255)
                                                                     ttttGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTaa                                                                             <  1:842868/150‑1 (MQ=255)
                                                                       ttGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAaca                                                                           >  2:1359940/1‑150 (MQ=255)
                                                                         gTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACg                                                                         <  2:22899/150‑1 (MQ=255)
                                                                            aCGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGccc                                                                      >  1:318935/1‑150 (MQ=255)
                                                                              gccagccaGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAg                                                                    >  1:450386/1‑150 (MQ=255)
                                                                                  gccagcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCaaa                                                                >  2:682547/1‑150 (MQ=255)
                                                                                     agcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTATCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAAccc                                                             <  1:1033659/150‑1 (MQ=255)
                                                                                        gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                          <  1:1300677/150‑1 (MQ=255)
                                                                                        gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                          <  2:1190027/150‑1 (MQ=255)
                                                                                         tAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataa                                                         <  1:720265/150‑1 (MQ=255)
                                                                                          aGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataat                                                        <  1:1362378/150‑1 (MQ=255)
                                                                                             gATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGa                                                     <  1:651247/150‑1 (MQ=255)
                                                                                                   cTGTAAAGATATTCTGTGATTTCTCATAACTTCTCCCAAAGTCTGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAg                                               <  2:563848/150‑1 (MQ=255)
                                                                                                    tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                              >  2:469180/1‑150 (MQ=255)
                                                                                                    tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATAACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                              >  1:1485379/1‑150 (MQ=255)
                                                                                                     gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCATTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAggg                                             >  2:282424/1‑150 (MQ=255)
                                                                                                       aaaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCACACCCATAATAGAAATAAGGGtt                                           >  1:1062348/1‑150 (MQ=255)
                                                                                                        aaGAAATTCTGTGATTGCGCATAACTTCTCCCTACGTCGGGGGAATCTTCAATCTTCCCCAGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATTGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                          <  1:281853/150‑1 (MQ=255)
                                                                                                        aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                          >  2:1707357/1‑150 (MQ=255)
                                                                                                        aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                          <  1:30116/150‑1 (MQ=255)
                                                                                                        aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                          >  1:743991/1‑150 (MQ=255)
                                                                                                          gAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTcc                                        <  2:969591/150‑1 (MQ=255)
                                                                                                             aTTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATAGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATc                                     <  2:395670/150‑1 (MQ=255)
                                                                                                               tCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCAc                                   >  1:674756/1‑150 (MQ=255)
                                                                                                                 tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                 <  1:1483436/150‑1 (MQ=255)
                                                                                                                 tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                 <  1:1210167/150‑1 (MQ=255)
                                                                                                                 tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                 <  2:64945/150‑1 (MQ=255)
                                                                                                                 tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                 <  1:1476854/150‑1 (MQ=255)
                                                                                                                     aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCa                             <  1:144374/150‑1 (MQ=255)
                                                                                                                      ttGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTACTGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAg                            <  2:293631/150‑1 (MQ=255)
                                                                                                                            aTAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGc                      >  2:574967/1‑150 (MQ=255)
                                                                                                                                    tcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTc              >  2:1605459/1‑150 (MQ=255)
                                                                                                                                     cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTcc             >  1:161364/1‑149 (MQ=255)
                                                                                                                                     cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACG‑ACAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc            >  1:1474017/1‑150 (MQ=255)
                                                                                                                                     cccAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCa             <  1:784022/150‑1 (MQ=255)
                                                                                                                                      ccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc            <  2:931991/150‑1 (MQ=255)
                                                                                                                                       cAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAcc           >  1:1682871/1‑150 (MQ=255)
                                                                                                                                        aaCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACTGTCCAGGTACGCAACGTTTCACCa          <  2:450386/150‑1 (MQ=255)
                                                                                                                                            tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACCCGCCCCGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGACCAGGTACGCAACGTTTCACCAACCg      >  1:100141/1‑150 (MQ=255)
                                                                                                                                            tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCg      <  2:1062348/150‑1 (MQ=255)
                                                                                                                                             cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt     >  2:670473/1‑150 (MQ=255)
                                                                                                                                             cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt     >  1:1072021/1‑150 (MQ=255)
                                                                                                                                               ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCCCCGTCCAGGTACGCAACGTTTCACCAACCGTCa   >  1:217338/1‑150 (MQ=255)
                                                                                                                                                gggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAg  >  1:1131138/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAG  >  NC_000913/4491014‑4491307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑