Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,491,167 C→G 7.5% intergenic (‑28/+39) idnR ← / ← idnT transcriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,1670CG 7.5% 337.9 / 13.3 104intergenic (‑28/+39)idnR/idnTtranscriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base C (43/53);  new base G (3/5);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

Only 100 of 119 total aligned reads displayed.

GCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAT  >  NC_000913/4491018‑4491315
                                                                                                                                                     |                                                                                                                                                    
gCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttc                                                                                                                                                      >  2:1362378/1‑150 (MQ=255)
 cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                                     >  2:651247/1‑150 (MQ=255)
 cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                                     <  1:1312295/150‑1 (MQ=255)
 cGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttca                                                                                                                                                     <  1:941102/150‑1 (MQ=255)
  gATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaa                                                                                                                                                    >  2:576662/1‑150 (MQ=255)
   aTGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaat                                                                                                                                                   <  2:777507/150‑1 (MQ=255)
    tGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatc                                                                                                                                                  >  1:395670/1‑150 (MQ=255)
         tCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                             <  1:1074916/150‑1 (MQ=255)
         gcGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGAGGATATAACGACTCACGGTCATTTTTGATACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                             <  1:83760/149‑1 (MQ=255)
            ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGCCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                                 >  2:814592/1‑143 (MQ=255)
            ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                                 <  1:814592/143‑1 (MQ=255)
             cTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                         <  1:1030544/150‑1 (MQ=255)
             cTGTTTCCATTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACCTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                         <  2:109105/150‑1 (MQ=255)
               gTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGaaa                                                                                                                                       >  2:1539903/1‑150 (MQ=255)
                  tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                    >  2:1476854/1‑150 (MQ=255)
                  tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                    >  2:1418636/1‑150 (MQ=255)
                  tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                    >  2:1483436/1‑150 (MQ=255)
                   ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCTCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                   >  1:293631/1‑150 (MQ=255)
                   ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                   >  1:920054/1‑150 (MQ=255)
                    cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                  <  2:942629/150‑1 (MQ=255)
                    cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                  <  2:920054/150‑1 (MQ=255)
                    cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGCTTCAATCTTCCCCCGAACCTAcc                                                                                                                                  >  2:842868/1‑149 (MQ=255)
                     ttttCCACCTTTTTCGTCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTATAGATATACTGTAACGAAATTCTGTGTTTGCGCATAACTTCTCCCAACGTCGGGGGTATCTTGAATCTTCCCCCGAAACTACAt                                                                                                                                 <  2:1043929/150‑1 (MQ=255)
                      ttGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACAta                                                                                                                                >  2:462026/1‑149 (MQ=255)
                       tGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATca                                                                                                                               >  1:1621053/1‑150 (MQ=255)
                        gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                              >  1:64945/1‑150 (MQ=255)
                        gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGAGATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGGCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                              <  1:126153/150‑1 (MQ=255)
                        gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGGCATTTTTGTTACGCCAGCCAGCGTAGCGATAACCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCATCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                              <  2:431083/150‑1 (MQ=255)
                         ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                             >  2:720265/1‑150 (MQ=255)
                         ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                             <  1:630525/150‑1 (MQ=255)
                         ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCACCGAAACTACATcaca                                                                                                                             >  2:717126/1‑150 (MQ=255)
                             cTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                         <  2:1662616/150‑1 (MQ=255)
                             cTTTTTAGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                         >  1:898967/1‑150 (MQ=255)
                                 ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                                     <  1:1514924/150‑1 (MQ=255)
                                 ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                                     <  1:790315/150‑1 (MQ=255)
                                  tCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAt                                                                                                                    >  1:1190027/1‑150 (MQ=255)
                                   cGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAtt                                                                                                                   >  2:1445363/1‑150 (MQ=255)
                                    ggCGAGCGGATATAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAACCTACATCACAATAGTTTAtta                                                                                                                  >  2:78743/1‑149 (MQ=255)
                                        aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                              <  1:428956/150‑1 (MQ=255)
                                        aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                              <  1:1549497/150‑1 (MQ=255)
                                         gCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCa                                                                                                             <  2:898967/150‑1 (MQ=255)
                                            gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                          <  2:702616/150‑1 (MQ=255)
                                            gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                          <  1:909364/150‑1 (MQ=255)
                                            gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                          >  2:1210167/1‑150 (MQ=255)
                                             atatAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                         <  2:457101/150‑1 (MQ=255)
                                             atatAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                         <  2:1621053/150‑1 (MQ=255)
                                             atatAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                         <  1:78743/150‑1 (MQ=255)
                                                   cGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                  >  1:1345694/1‑150 (MQ=255)
                                                     aCTCACGGTCATTTTTGTTACTCCAGCAAGCGTAGAGATATCATGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTAGGGTGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                                 <  1:1393823/150‑1 (MQ=255)
                                                     aCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                                 <  1:828087/150‑1 (MQ=255)
                                                       tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                               <  1:462026/150‑1 (MQ=255)
                                                       tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCACATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                               <  1:559558/150‑1 (MQ=255)
                                                           ggTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAAt                                                                                           >  2:1662601/1‑150 (MQ=255)
                                                             tCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATgg                                                                                         <  1:1237550/150‑1 (MQ=255)
                                                               aTTTTTGTTACGCCAGCCAGCGTATCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                       <  2:1497720/150‑1 (MQ=255)
                                                               aTTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                       <  2:828855/150‑1 (MQ=255)
                                                                     gTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACg                                                                                 <  2:22899/150‑1 (MQ=255)
                                                                       tACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGcc                                                                               >  1:1542396/1‑150 (MQ=255)
                                                                          gccagccaGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAg                                                                            >  1:450386/1‑150 (MQ=255)
                                                                           ccagccagCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGc                                                                           <  2:838384/150‑1 (MQ=255)
                                                                              gccagcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCaaa                                                                        >  2:682547/1‑150 (MQ=255)
                                                                                 agcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTATCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAAccc                                                                     <  1:1033659/150‑1 (MQ=255)
                                                                                    gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                                  <  2:1190027/150‑1 (MQ=255)
                                                                                     tAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataa                                                                 <  1:720265/150‑1 (MQ=255)
                                                                                      aGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataat                                                                <  1:1362378/150‑1 (MQ=255)
                                                                                         gATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGa                                                             <  1:651247/150‑1 (MQ=255)
                                                                                             tCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATa                                                         <  2:318935/150‑1 (MQ=255)
                                                                                               cTGTAAAGATATTCTGTGATTTCTCATAACTTCTCCCAAAGTCTGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAg                                                       <  2:563848/150‑1 (MQ=255)
                                                                                                tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATAACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                                      >  1:1485379/1‑150 (MQ=255)
                                                                                                 gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCATTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAggg                                                     >  2:282424/1‑150 (MQ=255)
                                                                                                   aaaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCACACCCATAATAGAAATAAGGGtt                                                   >  1:1062348/1‑150 (MQ=255)
                                                                                                    aaGAAATTCTGTGATTGCGCATAACTTCTCCCTACGTCGGGGGAATCTTCAATCTTCCCCAGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATTGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                  <  1:281853/150‑1 (MQ=255)
                                                                                                    aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                  >  2:1707357/1‑150 (MQ=255)
                                                                                                    aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                  >  1:743991/1‑150 (MQ=255)
                                                                                                    aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                  <  1:30116/150‑1 (MQ=255)
                                                                                                      gAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTcc                                                <  2:969591/150‑1 (MQ=255)
                                                                                                         aTTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATAGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATc                                             <  2:395670/150‑1 (MQ=255)
                                                                                                           tCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCAc                                           >  1:674756/1‑150 (MQ=255)
                                                                                                             tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                         <  1:1483436/150‑1 (MQ=255)
                                                                                                             tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                         <  1:1210167/150‑1 (MQ=255)
                                                                                                             tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                         <  2:64945/150‑1 (MQ=255)
                                                                                                             tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                         <  1:1476854/150‑1 (MQ=255)
                                                                                                                  ttGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTACTGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAg                                    <  2:293631/150‑1 (MQ=255)
                                                                                                                        aTAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGc                              >  2:574967/1‑150 (MQ=255)
                                                                                                                                tcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTc                      >  2:1605459/1‑150 (MQ=255)
                                                                                                                                 cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTcc                     >  1:161364/1‑149 (MQ=255)
                                                                                                                                 cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGACAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                    >  1:1474017/1‑150 (MQ=255)
                                                                                                                                  ccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                    <  2:931991/150‑1 (MQ=255)
                                                                                                                                   cAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAcc                   >  1:1682871/1‑150 (MQ=255)
                                                                                                                                    aaCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACTGTCCAGGTACGCAACGTTTCACCa                  <  2:450386/150‑1 (MQ=255)
                                                                                                                                        tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACCCGCCCCGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGACCAGGTACGCAACGTTTCACCAACCg              >  1:100141/1‑150 (MQ=255)
                                                                                                                                        tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCg              <  2:1062348/150‑1 (MQ=255)
                                                                                                                                         cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt             >  1:1072021/1‑150 (MQ=255)
                                                                                                                                          gggggAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTc            >  2:506945/1‑150 (MQ=255)
                                                                                                                                           ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCCCCGTCCAGGTACGCAACGTTTCACCAACCGTCa           >  1:217338/1‑150 (MQ=255)
                                                                                                                                                  cttcaatcttcCCCCGAAACTACATCACAATTTTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa    >  1:1525466/1‑150 (MQ=255)
                                                                                                                                                  cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa    >  1:1560350/1‑150 (MQ=255)
                                                                                                                                                  cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa    >  1:1274193/1‑150 (MQ=255)
                                                                                                                                                   ttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa   >  1:925804/1‑150 (MQ=255)
                                                                                                                                                    tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt  >  1:1346860/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAT  >  NC_000913/4491018‑4491315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: