Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,491,170 T→G 6.7% intergenic (‑31/+36) idnR ← / ← idnT transcriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,1700TG 6.7% 317.3 / 11.7 104intergenic (‑31/+36)idnR/idnTtranscriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (43/54);  new base G (3/4);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 115 total aligned reads displayed.

ATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCC  >  NC_000913/4491021‑4491319
                                                                                                                                                     |                                                                                                                                                     
aTGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaat                                                                                                                                                       <  2:777507/150‑1 (MQ=255)
 tGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatc                                                                                                                                                      >  1:395670/1‑150 (MQ=255)
      tCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                                 <  1:1074916/150‑1 (MQ=255)
      gcGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGAGGATATAACGACTCACGGTCATTTTTGATACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                                 <  1:83760/149‑1 (MQ=255)
         ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGCCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                                     >  2:814592/1‑143 (MQ=255)
          cTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                             <  1:1030544/150‑1 (MQ=255)
          cTGTTTCCATTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACCTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                             <  2:109105/150‑1 (MQ=255)
           tGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                            <  2:369744/150‑2 (MQ=255)
           ggTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                            <  2:862642/149‑2 (MQ=255)
            gTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGaaa                                                                                                                                           >  2:1539903/1‑150 (MQ=255)
               tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                        >  2:1483436/1‑150 (MQ=255)
               tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                        >  2:1476854/1‑150 (MQ=255)
                ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCTCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                       >  1:293631/1‑150 (MQ=255)
                ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                       >  1:920054/1‑150 (MQ=255)
                 cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      <  2:920054/150‑1 (MQ=255)
                 cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      <  2:942629/150‑1 (MQ=255)
                 cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      >  2:1679774/1‑150 (MQ=255)
                  ttttCCACCTTTTTCGTCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTATAGATATACTGTAACGAAATTCTGTGTTTGCGCATAACTTCTCCCAACGTCGGGGGTATCTTGAATCTTCCCCCGAAACTACAt                                                                                                                                     <  2:1043929/150‑1 (MQ=255)
                   ttGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACAta                                                                                                                                    >  2:462026/1‑149 (MQ=255)
                    tGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATca                                                                                                                                   >  1:1621053/1‑150 (MQ=255)
                     gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  >  1:64945/1‑150 (MQ=255)
                     gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGAGATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGGCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  <  1:126153/150‑1 (MQ=255)
                     gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGGCATTTTTGTTACGCCAGCCAGCGTAGCGATAACCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCATCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  <  2:431083/150‑1 (MQ=255)
                      ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                                 <  1:630525/150‑1 (MQ=255)
                      ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                                 >  2:720265/1‑150 (MQ=255)
                          cTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                             <  2:1662616/150‑1 (MQ=255)
                          cTTTTTAGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                             >  1:898967/1‑150 (MQ=255)
                              ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                                         <  1:790315/150‑1 (MQ=255)
                                cGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAtt                                                                                                                       >  2:1445363/1‑150 (MQ=255)
                                 ggCGAGCGGATATAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAACCTACATCACAATAGTTTAtta                                                                                                                      >  2:78743/1‑149 (MQ=255)
                                     aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                                  <  1:1549497/150‑1 (MQ=255)
                                      gCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCa                                                                                                                 <  2:898967/150‑1 (MQ=255)
                                         gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              >  2:1210167/1‑150 (MQ=255)
                                         gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              <  2:702616/150‑1 (MQ=255)
                                         gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              <  1:909364/150‑1 (MQ=255)
                                          atatAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                             <  2:457101/150‑1 (MQ=255)
                                          atatAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                             <  1:78743/150‑1 (MQ=255)
                                                cGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTC‑GGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                      >  1:1345694/1‑150 (MQ=255)
                                                 gACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                      <  1:1539903/150‑1 (MQ=255)
                                                 gACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                      >  2:784022/1‑150 (MQ=255)
                                                  aCTCACGGTCATTTTTGTTACTCCAGCAAGCGTAGAGATATCATGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTAGGGTGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                                     <  1:1393823/150‑1 (MQ=255)
                                                  aCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                                     <  1:828087/150‑1 (MQ=255)
                                                    tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                                   <  1:462026/150‑1 (MQ=255)
                                                    tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCACATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                                   <  1:559558/150‑1 (MQ=255)
                                                        ggTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAAt                                                                                               >  2:1662601/1‑150 (MQ=255)
                                                          tCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATgg                                                                                             <  1:1237550/150‑1 (MQ=255)
                                                            aTTTTTGTTACGCCAGCCAGCGTATCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                           <  2:1497720/150‑1 (MQ=255)
                                                            aTTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                           <  2:828855/150‑1 (MQ=255)
                                                              ttttGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTaa                                                                                         <  1:842868/150‑1 (MQ=255)
                                                                ttGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAaca                                                                                       >  2:1359940/1‑150 (MQ=255)
                                                                  gTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACg                                                                                     <  2:22899/150‑1 (MQ=255)
                                                                    tACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGcc                                                                                   >  1:1542396/1‑150 (MQ=255)
                                                                     aCGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGccc                                                                                  >  1:318935/1‑150 (MQ=255)
                                                                       gccagccaGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAg                                                                                >  1:450386/1‑150 (MQ=255)
                                                                        ccagccagCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGc                                                                               <  2:838384/150‑1 (MQ=255)
                                                                           gccagcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCaaa                                                                            >  2:682547/1‑150 (MQ=255)
                                                                              agcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTATCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAAccc                                                                         <  1:1033659/150‑1 (MQ=255)
                                                                                 gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                                      <  1:1300677/150‑1 (MQ=255)
                                                                                 gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                                      <  2:1190027/150‑1 (MQ=255)
                                                                                  tAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataa                                                                     <  1:720265/150‑1 (MQ=255)
                                                                                            cTGTAAAGATATTCTGTGATTTCTCATAACTTCTCCCAAAGTCTGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAg                                                           <  2:563848/150‑1 (MQ=255)
                                                                                             tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATAACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                                          >  1:1485379/1‑150 (MQ=255)
                                                                                              gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCATTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAggg                                                         >  2:282424/1‑150 (MQ=255)
                                                                                                aaaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCACACCCATAATAGAAATAAGGGtt                                                       >  1:1062348/1‑150 (MQ=255)
                                                                                                 aaGAAATTCTGTGATTGCGCATAACTTCTCCCTACGTCGGGGGAATCTTCAATCTTCCCCAGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATTGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      <  1:281853/150‑1 (MQ=255)
                                                                                                 aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      <  1:30116/150‑1 (MQ=255)
                                                                                                 aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      >  2:1707357/1‑150 (MQ=255)
                                                                                                 aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      >  1:743991/1‑150 (MQ=255)
                                                                                                   gAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTcc                                                    <  2:969591/150‑1 (MQ=255)
                                                                                                      aTTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATAGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATc                                                 <  2:395670/150‑1 (MQ=255)
                                                                                                        tCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCAc                                               >  1:674756/1‑150 (MQ=255)
                                                                                                          tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  1:1476854/150‑1 (MQ=255)
                                                                                                          tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  1:1483436/150‑1 (MQ=255)
                                                                                                          tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  2:64945/150‑1 (MQ=255)
                                                                                                          tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  1:1210167/150‑1 (MQ=255)
                                                                                                              aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCa                                         <  1:144374/150‑1 (MQ=255)
                                                                                                              aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCa                                         <  1:584113/150‑1 (MQ=255)
                                                                                                               ttGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTACTGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAg                                        <  2:293631/150‑1 (MQ=255)
                                                                                                                     aTAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGc                                  >  2:574967/1‑150 (MQ=255)
                                                                                                                             tcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTc                          >  2:1605459/1‑150 (MQ=255)
                                                                                                                              cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTcc                         >  1:161364/1‑149 (MQ=255)
                                                                                                                              cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACG‑ACAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                        >  1:1474017/1‑150 (MQ=255)
                                                                                                                              cccAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCa                         <  1:784022/150‑1 (MQ=255)
                                                                                                                               ccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                        <  2:931991/150‑1 (MQ=255)
                                                                                                                                cAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAcc                       >  1:1682871/1‑150 (MQ=255)
                                                                                                                                 aaCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACTGTCCAGGTACGCAACGTTTCACCa                      <  2:450386/150‑1 (MQ=255)
                                                                                                                                     tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACCCGCCCCGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGACCAGGTACGCAACGTTTCACCAACCg                  >  1:100141/1‑150 (MQ=255)
                                                                                                                                     tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCg                  <  2:1062348/150‑1 (MQ=255)
                                                                                                                                      cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                 >  2:670473/1‑150 (MQ=255)
                                                                                                                                      cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                 >  1:1072021/1‑150 (MQ=255)
                                                                                                                                       gggggAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTc                >  2:506945/1‑150 (MQ=255)
                                                                                                                                        ggggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCCCCGTCCAGGTACGCAACGTTTCACCAACCGTCa               >  1:217338/1‑150 (MQ=255)
                                                                                                                                         gggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAg              >  1:1131138/1‑150 (MQ=255)
                                                                                                                                               cttcaatcttcCCCCGAAACTACATCACAATTTTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1525466/1‑150 (MQ=255)
                                                                                                                                               cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1274193/1‑150 (MQ=255)
                                                                                                                                               cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1560350/1‑150 (MQ=255)
                                                                                                                                                ttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa       >  1:925804/1‑150 (MQ=255)
                                                                                                                                                 tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt      <  2:1513290/150‑1 (MQ=255)
                                                                                                                                                 tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt      >  1:1346860/1‑150 (MQ=255)
                                                                                                                                                     tcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAccc  <  1:682547/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCC  >  NC_000913/4491021‑4491319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑