Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,491,171 C→A 6.3% intergenic (‑32/+35) idnR ← / ← idnT transcriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,491,1710CA 6.3% 328.3 / 7.5 104intergenic (‑32/+35)idnR/idnTtranscriptional repressor, 5‑gluconate‑binding/L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base C (43/54);  new base A (3/4);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCC  >  NC_000913/4491022‑4491319
                                                                                                                                                     |                                                                                                                                                    
tGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatc                                                                                                                                                      >  1:395670/1‑150 (MQ=255)
    cTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTcc                                                                                                                                                  <  1:1633531/150‑1 (MQ=255)
     tCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                                 <  1:1074916/150‑1 (MQ=255)
     gcGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGAGGATATAACGACTCACGGTCATTTTTGATACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTccc                                                                                                                                                 <  1:83760/149‑1 (MQ=255)
        ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGCCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                                     >  2:814592/1‑143 (MQ=255)
        ccTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGaatcttcaatct                                                                                                                                                     <  1:814592/143‑1 (MQ=255)
         cTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                             <  1:1030544/150‑1 (MQ=255)
         cTGTTTCCATTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACCTCGGGGGAAGATTGAAGATTCCCCCGa                                                                                                                                             <  2:109105/150‑1 (MQ=255)
          tGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                            <  2:369744/150‑2 (MQ=255)
          ggTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGATTGAAGATTCCCCCGac                                                                                                                                            <  2:862642/149‑2 (MQ=255)
           gTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGaaa                                                                                                                                           >  2:1539903/1‑150 (MQ=255)
              tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                        >  2:1418636/1‑150 (MQ=255)
              tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                        >  2:1476854/1‑150 (MQ=255)
              tCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTa                                                                                                                                        >  2:1483436/1‑150 (MQ=255)
               ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCTCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                       >  1:293631/1‑150 (MQ=255)
               ccTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTAc                                                                                                                                       >  1:920054/1‑150 (MQ=255)
                cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      <  2:942629/150‑1 (MQ=255)
                cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      <  2:920054/150‑1 (MQ=255)
                cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAAGCTTCAATCTTCCCCCGAACCTAcc                                                                                                                                      >  2:842868/1‑149 (MQ=255)
                cTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACa                                                                                                                                      >  2:1679774/1‑150 (MQ=255)
                 ttttCCACCTTTTTCGTCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTATAGATATACTGTAACGAAATTCTGTGTTTGCGCATAACTTCTCCCAACGTCGGGGGTATCTTGAATCTTCCCCCGAAACTACAt                                                                                                                                     <  2:1043929/150‑1 (MQ=255)
                  ttGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACAta                                                                                                                                    >  2:462026/1‑149 (MQ=255)
                   tGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATca                                                                                                                                   >  1:1621053/1‑150 (MQ=255)
                    gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  >  1:64945/1‑150 (MQ=255)
                    gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGAGATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGGCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  <  1:126153/150‑1 (MQ=255)
                    gCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGGCATTTTTGTTACGCCAGCCAGCGTAGCGATAACCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCATCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcac                                                                                                                                  <  2:431083/150‑1 (MQ=255)
                     ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                                 <  1:630525/150‑1 (MQ=255)
                     ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATcaca                                                                                                                                 >  2:720265/1‑150 (MQ=255)
                     ccACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCACCGAAACTACATcaca                                                                                                                                 >  2:717126/1‑150 (MQ=255)
                      cACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACaa                                                                                                                                >  1:953156/1‑150 (MQ=255)
                         cTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                             <  2:1662616/150‑1 (MQ=255)
                         cTTTTTAGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAatat                                                                                                                             >  1:898967/1‑150 (MQ=255)
                             ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                                         <  1:790315/150‑1 (MQ=255)
                             ttCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTa                                                                                                                         <  1:1514924/150‑1 (MQ=255)
                              tCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAt                                                                                                                        >  1:1190027/1‑150 (MQ=255)
                               cGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTAtt                                                                                                                       >  2:1445363/1‑150 (MQ=255)
                                ggCGAGCGGATATAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAACCTACATCACAATAGTTTAtta                                                                                                                      >  2:78743/1‑149 (MQ=255)
                                    aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                                  <  1:1549497/150‑1 (MQ=255)
                                    aGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTc                                                                                                                  <  1:428956/150‑1 (MQ=255)
                                     gCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCa                                                                                                                 <  2:898967/150‑1 (MQ=255)
                                        gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              >  2:1210167/1‑150 (MQ=255)
                                        gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              <  2:702616/150‑1 (MQ=255)
                                        gATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTg                                                                                                              <  1:909364/150‑1 (MQ=255)
                                         atatAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                             <  2:1621053/150‑1 (MQ=255)
                                         atatAACGACTCACGGTCATTCTTGTTACGCCAGCCAACGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGc                                                                                                             <  1:78743/150‑1 (MQ=255)
                                               cGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTC‑GGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                      >  1:1345694/1‑150 (MQ=255)
                                                gACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACgg                                                                                                      >  2:784022/1‑150 (MQ=255)
                                                 aCTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGc                                                                                                     <  1:828087/150‑1 (MQ=255)
                                                   tCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCACATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGt                                                                                                   <  1:559558/150‑1 (MQ=255)
                                                       ggTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAAt                                                                                               >  2:1662601/1‑150 (MQ=255)
                                                         tCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATgg                                                                                             <  1:1237550/150‑1 (MQ=255)
                                                           aTTTTTGTTACGCCAGCCAGCGTATCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                           <  2:1497720/150‑1 (MQ=255)
                                                           aTTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCt                                                                                           <  2:828855/150‑1 (MQ=255)
                                                               ttGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAaca                                                                                       >  2:1359940/1‑150 (MQ=255)
                                                                   tACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGcc                                                                                   >  1:1542396/1‑150 (MQ=255)
                                                                    aCGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGccc                                                                                  >  1:318935/1‑150 (MQ=255)
                                                                      gccagccaGCGTAGCGATATCCTGTAAAGAAATTCTGTGATAGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAg                                                                                >  1:450386/1‑150 (MQ=255)
                                                                             agcGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTATCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAAccc                                                                         <  1:1033659/150‑1 (MQ=255)
                                                                                gTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCata                                                                      <  1:1300677/150‑1 (MQ=255)
                                                                                  aGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCataat                                                                    <  1:1362378/150‑1 (MQ=255)
                                                                                     gATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGa                                                                 <  1:651247/150‑1 (MQ=255)
                                                                                         tCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATa                                                             <  2:318935/150‑1 (MQ=255)
                                                                                           cTGTAAAGATATTCTGTGATTTCTCATAACTTCTCCCAAAGTCTGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAg                                                           <  2:563848/150‑1 (MQ=255)
                                                                                            tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                                          >  2:469180/1‑150 (MQ=255)
                                                                                            tGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATAACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAgg                                                          >  1:1485379/1‑150 (MQ=255)
                                                                                             gTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCATTACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAggg                                                         >  2:282424/1‑150 (MQ=255)
                                                                                               aaaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCACACCCATAATAGAAATAAGGGtt                                                       >  1:1062348/1‑150 (MQ=255)
                                                                                                aaGAAATTCTGTGATTGCGCATAACTTCTCCCTACGTCGGGGGAATCTTCAATCTTCCCCAGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATTGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      <  1:281853/150‑1 (MQ=255)
                                                                                                aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      >  2:1707357/1‑150 (MQ=255)
                                                                                                aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      >  1:743991/1‑150 (MQ=255)
                                                                                                aaGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGttt                                                      <  1:30116/150‑1 (MQ=255)
                                                                                                  gAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTcc                                                    <  2:969591/150‑1 (MQ=255)
                                                                                                     aTTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATAGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATc                                                 <  2:395670/150‑1 (MQ=255)
                                                                                                       tCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCAc                                               >  1:674756/1‑150 (MQ=255)
                                                                                                         tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  2:64945/150‑1 (MQ=255)
                                                                                                         tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  1:1483436/150‑1 (MQ=255)
                                                                                                         tgtgATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCg                                             <  1:1476854/150‑1 (MQ=255)
                                                                                                             aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCa                                         <  1:144374/150‑1 (MQ=255)
                                                                                                             aTTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCa                                         <  1:584113/150‑1 (MQ=255)
                                                                                                              ttGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTACTGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAg                                        <  2:293631/150‑1 (MQ=255)
                                                                                                                    aTAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGc                                  >  2:574967/1‑150 (MQ=255)
                                                                                                                            tcCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTc                          >  2:1605459/1‑150 (MQ=255)
                                                                                                                             cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCTTAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTcc                         >  1:161364/1‑149 (MQ=255)
                                                                                                                             cccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACG‑ACAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                        >  1:1474017/1‑150 (MQ=255)
                                                                                                                             cccAACGTCGGGGGAATCTCCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCa                         <  1:784022/150‑1 (MQ=255)
                                                                                                                              ccAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAc                        <  2:931991/150‑1 (MQ=255)
                                                                                                                               cAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCAcc                       >  1:1682871/1‑150 (MQ=255)
                                                                                                                                aaCGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACTGTCCAGGTACGCAACGTTTCACCa                      <  2:450386/150‑1 (MQ=255)
                                                                                                                                    tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACCCGCCCCGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGACCAGGTACGCAACGTTTCACCAACCg                  >  1:100141/1‑150 (MQ=255)
                                                                                                                                    tCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCg                  <  2:1062348/150‑1 (MQ=255)
                                                                                                                                     cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                 >  2:670473/1‑150 (MQ=255)
                                                                                                                                     cGGGGGAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGt                 >  1:1072021/1‑150 (MQ=255)
                                                                                                                                      gggggAAGATTGAAGATTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTc                >  2:506945/1‑150 (MQ=255)
                                                                                                                                        gggAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAg              >  1:1131138/1‑150 (MQ=255)
                                                                                                                                              cttcaatcttcCCCCGAAACTACATCACAATTTTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1525466/1‑150 (MQ=255)
                                                                                                                                              cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1560350/1‑150 (MQ=255)
                                                                                                                                              cttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaa        >  1:1274193/1‑150 (MQ=255)
                                                                                                                                               ttcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTaaaa       >  1:925804/1‑150 (MQ=255)
                                                                                                                                                tcaatcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAAt      >  1:1346860/1‑150 (MQ=255)
                                                                                                                                                    tcttcCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATAccc  <  1:682547/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCGGATATAACGACTCACGGTCATTTTTGTTACGCCAGCCAGCGTAGCGATATCCTGTAAAGAAATTCTGTGATTGCGCATAACTTCTCCCAACGTCGGGGGAATCTTCAATCTTCCCCCGAAACTACATCACAATATTTTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCC  >  NC_000913/4491022‑4491319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑